##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295328_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3389789
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.878779475654678	31.0	30.0	33.0	25.0	34.0
2	29.64169008749512	31.0	30.0	34.0	24.0	34.0
3	30.231591700840376	31.0	30.0	34.0	25.0	34.0
4	33.29669486802866	35.0	35.0	37.0	26.0	37.0
5	34.0604842956302	35.0	35.0	37.0	30.0	37.0
6	34.083161223309176	37.0	35.0	37.0	29.0	37.0
7	34.06905149553556	37.0	35.0	37.0	29.0	37.0
8	34.004584946142664	37.0	35.0	37.0	29.0	37.0
9	34.913793454400846	39.0	35.0	39.0	26.0	39.0
10	34.85486736785092	39.0	35.0	39.0	27.0	39.0
11	35.32244868338413	39.0	35.0	39.0	27.0	39.0
12	35.3185782359905	39.0	35.0	39.0	27.0	39.0
13	35.08108823292541	39.0	35.0	39.0	27.0	39.0
14	35.32134595988128	39.0	34.0	41.0	24.0	41.0
15	35.86928920944637	39.0	35.0	41.0	27.0	41.0
16	35.867957858143974	39.0	35.0	41.0	27.0	41.0
17	35.915011229312505	39.0	35.0	41.0	27.0	41.0
18	35.85369856353891	39.0	35.0	41.0	27.0	41.0
19	35.92991333678881	39.0	35.0	41.0	27.0	41.0
20	35.979208735410964	39.0	35.0	41.0	27.0	41.0
21	35.98439342389747	39.0	35.0	41.0	27.0	41.0
22	35.79146932154184	39.0	34.0	41.0	26.0	41.0
23	35.43196139936734	39.0	34.0	41.0	25.0	41.0
24	35.77759707167614	39.0	34.0	41.0	26.0	41.0
25	34.73489972384712	38.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5535667672768483
1101	2	0.7241096684589756
1101	3	0.605577833529285
1101	4	0.6130204869676277
1101	5	0.46521069811431204
1101	6	0.5325311560273605
1101	7	0.49999359217153483
1101	8	0.40526617121378194
1101	9	0.4868397740518091
1101	10	0.8256064230189111
1101	11	0.7151590263544634
1101	12	0.7927486184690267
1101	13	0.8100393701783517
1101	14	1.4408741880259868
1101	15	1.0258306214881259
1101	16	0.8553895539552556
1101	17	1.100184223415873
1101	18	1.0831062326867027
1101	19	1.1232136986066976
1101	20	1.1481563868488678
1101	21	1.1281539854845732
1101	22	0.9346957980613695
1101	23	-1.4103154747732773
1101	24	0.3620345648399095
1101	25	0.9881442146080914
1102	1	0.457867922041423
1102	2	0.49753881925439103
1102	3	0.4612640903054199
1102	4	0.6225541993917787
1102	5	0.3785844593219565
1102	6	0.43025717065084734
1102	7	0.4264899783095899
1102	8	0.4335874069741408
1102	9	0.6712376335734405
1102	10	0.9835299014402636
1102	11	0.7185116530029063
1102	12	0.7714304240635528
1102	13	0.6755535959903938
1102	14	1.4374108482446317
1102	15	0.9861156101173236
1102	16	0.8457120153567743
1102	17	1.090476461141094
1102	18	1.065662997933309
1102	19	1.0696759645532126
1102	20	1.1639442905722888
1102	21	1.008995054901888
1102	22	1.0217512222298026
1102	23	1.1069446751306558
1102	24	1.0562130628451953
1102	25	1.1735715136415124
1103	1	-0.22415377094209177
1103	2	-1.3445195424680136
1103	3	-0.3105781428242764
1103	4	0.013835652163777468
1103	5	-0.03710832548501486
1103	6	0.09646044401272036
1103	7	0.07994543588264236
1103	8	0.26370152623533727
1103	9	0.760804544067085
1103	10	0.9194172492782542
1103	11	0.5582429153171518
1103	12	0.4470857359755769
1103	13	0.2157804340381304
1103	14	-0.7721346880143045
1103	15	0.015602206011138264
1103	16	0.06920825082843152
1103	17	0.5966401035950568
1103	18	0.6004478458851708
1103	19	0.6837058265190521
1103	20	0.548365019967072
1103	21	0.7504212509067685
1103	22	0.33012161262134043
1103	23	1.131893373762395
1103	24	0.919823923574782
1103	25	0.6962694008574601
1104	1	0.10245080633474402
1104	2	0.11117774684116455
1104	3	-0.19629670399027788
1104	4	-0.10446228654890888
1104	5	0.12606359651510957
1104	6	0.09058481541863728
1104	7	0.014353672540373452
1104	8	0.18090497899909508
1104	9	-2.0251994975213776
1104	10	-0.28368531341464376
1104	11	-0.23611394367276262
1104	12	0.23591093240017358
1104	13	0.2764883350283611
1104	14	-1.6678028785199217
1104	15	-0.3416484528859485
1104	16	0.040454402983741033
1104	17	0.49478855094051966
1104	18	0.44425292665582816
1104	19	0.4691596511209255
1104	20	0.6394522322048033
1104	21	0.6231683686758203
1104	22	0.7188782929527946
1104	23	1.1839675344414928
1104	24	0.8442540278814548
1104	25	0.7802098788499947
1105	1	-0.2576117488119962
1105	2	-0.11684707890064772
1105	3	-1.4237677829929645
1105	4	-0.44953983523355134
1105	5	-0.2306098204585254
1105	6	0.004331389541363251
1105	7	-0.03647725929402412
1105	8	-0.038908147765880585
1105	9	0.3098612962579068
1105	10	0.4797973274711964
1105	11	-0.10871413032380417
1105	12	-0.08270625984932423
1105	13	-0.19529024577776255
1105	14	0.6083232399835623
1105	15	0.34774959632986935
1105	16	-0.05583813767314183
1105	17	0.3443401135840034
1105	18	0.3794095256992662
1105	19	0.36281177657228625
1105	20	-0.3256461603827461
1105	21	0.2260793617978365
1105	22	0.3397233115079743
1105	23	0.5302158123913259
1105	24	0.378177215010119
1105	25	0.5459747269432427
1106	1	-1.468499642784483
1106	2	-1.0096000526857303
1106	3	-1.038568079212407
1106	4	-1.2801027148273647
1106	5	-0.8504469498866243
1106	6	-0.5346363283033497
1106	7	-0.611017975057436
1106	8	-0.6192682974794934
1106	9	-0.2859784996463972
1106	10	-1.8052334410077435
1106	11	-1.1387508319629092
1106	12	-0.8591085970257382
1106	13	-0.8438381063942302
1106	14	-0.14133469171805757
1106	15	-0.8783846271654454
1106	16	-1.3673682163517356
1106	17	-0.5967068542390948
1106	18	-0.5640103553367979
1106	19	-0.43633655493786705
1106	20	-0.15426657220796614
1106	21	-0.07991286682743493
1106	22	-0.21556414289691617
1106	23	-0.42637956864035687
1106	24	-0.7704111189058764
1106	25	-1.4573633804851696
1107	1	0.07163446041768395
1107	2	0.19388302614264674
1107	3	0.06255747271561418
1107	4	0.19646009348407745
1107	5	0.04719764890126754
1107	6	0.07797149838835793
1107	7	0.031478969265705814
1107	8	-0.11969080112435648
1107	9	0.4035893617956958
1107	10	0.11223070533203128
1107	11	0.006194535538554646
1107	12	-0.10794060064865363
1107	13	0.0622651142545152
1107	14	-0.9438647690515154
1107	15	-0.4320039579895436
1107	16	-0.14111716794842977
1107	17	0.022360939042343375
1107	18	0.2955027234222598
1107	19	0.22752295555309843
1107	20	0.2832399492649529
1107	21	0.4511240762480213
1107	22	-0.14008206221780028
1107	23	0.6954293458492131
1107	24	0.485536256446764
1107	25	0.1356932436908309
1108	1	-0.04671118896595061
1108	2	0.27046938497769446
1108	3	0.04863108753563239
1108	4	-0.2086726244499033
1108	5	-0.18565596810316265
1108	6	-0.0460032738056384
1108	7	-0.08420982915154696
1108	8	-0.0972949658820994
1108	9	0.41504830980146323
1108	10	0.3796052791693185
1108	11	0.152296049309534
1108	12	0.09811932125480638
1108	13	0.04236354492019956
1108	14	0.36821754384921945
1108	15	0.21963314453653737
1108	16	0.15564190635763708
1108	17	0.2819870667104638
1108	18	0.3201608953006172
1108	19	0.37170562844266186
1108	20	0.48879606273436593
1108	21	0.4441416417383479
1108	22	0.34259727797127226
1108	23	0.3062125176359416
1108	24	0.2766851933032157
1108	25	-0.4417399062150835
1109	1	-0.8725336486039623
1109	2	-0.6043046655648929
1109	3	-0.789469871180156
1109	4	-0.6805678178640875
1109	5	-0.9354981693016029
1109	6	-1.1834074609385752
1109	7	-0.7831320642401494
1109	8	-1.159255428565892
1109	9	-0.9470288535415179
1109	10	-1.0557604401023681
1109	11	-1.087160154522799
1109	12	-1.1221553407920055
1109	13	-1.1342020233271057
1109	14	-0.761547085734577
1109	15	-1.1656802762199234
1109	16	-1.3886272060850757
1109	17	-1.4072005991498955
1109	18	-1.9969431579698025
1109	19	-2.0503948750321683
1109	20	-1.7526453168625267
1109	21	-1.5839902625237556
1109	22	-1.656201211062296
1109	23	-1.1048296047985886
1109	24	-1.9811472093754787
1109	25	-1.096865251033016
1110	1	0.04699068438193876
1110	2	0.2142131254344548
1110	3	0.0589993539089555
1110	4	0.1308548518664452
1110	5	0.15312282579573377
1110	6	0.10528475548492366
1110	7	0.013645918135303248
1110	8	-0.0777044426253326
1110	9	0.27058708202797277
1110	10	0.15103713429270726
1110	11	0.10505189018168437
1110	12	0.1491331620284484
1110	13	0.15903650533525848
1110	14	-2.554287258554446
1110	15	-0.7439112756450541
1110	16	-0.2393898633896825
1110	17	-0.34194614293703296
1110	18	-2.5847055404128483
1110	19	-0.4725552939472166
1110	20	-1.8485871608688313
1110	21	-0.30303848534063604
1110	22	-1.7732104288604518
1110	23	-0.4296831922167854
1110	24	0.09075418373124222
1110	25	-0.0706823923004265
1111	1	-0.11570454404415997
1111	2	-1.2919644956441942
1111	3	-0.39111217104601437
1111	4	-0.019369321435640074
1111	5	-0.0678145969909778
1111	6	-0.0034189076562682885
1111	7	0.035569625052772835
1111	8	-0.01952289550623476
1111	9	-1.6057118899423202
1111	10	-0.3234952150829571
1111	11	-0.07737673322568384
1111	12	-0.0487214680952448
1111	13	0.04058578198385021
1111	14	0.8525818271244887
1111	15	0.27473639667525873
1111	16	0.20364438662208784
1111	17	-0.05518750088836555
1111	18	0.12241143409354294
1111	19	-0.11910865193525666
1111	20	0.2597444921566705
1111	21	-0.5648995703959585
1111	22	-0.18252063771610239
1111	23	0.017990205273868298
1111	24	-0.48110516476695864
1111	25	-1.9069561288993526
1112	1	0.13723554130334037
1112	2	0.3344329400656818
1112	3	0.022330123305145833
1112	4	0.263437421764543
1112	5	0.20120693747593066
1112	6	0.06424771223128545
1112	7	0.08642041792262489
1112	8	0.05361371991472197
1112	9	-0.36758516044714895
1112	10	0.365735691136841
1112	11	0.02631468067573195
1112	12	0.07473782387126704
1112	13	-0.09602160163865392
1112	14	0.5065823714961013
1112	15	0.14745530531673268
1112	16	0.15325560522882853
1112	17	-3.772916279200338
1112	18	-1.0431532141898359
1112	19	-1.243545519006176
1112	20	-0.1231029073310026
1112	21	-0.5341740913660971
1112	22	-1.5332586008090061
1112	23	-1.5839969317342053
1112	24	-0.4144176552056891
1112	25	-0.05778716256062921
1113	1	-0.3937187013598802
1113	2	-0.21107354516008314
1113	3	-0.19815105304041936
1113	4	-0.4835012384588424
1113	5	-0.2985410923466034
1113	6	-0.3589829873005712
1113	7	-0.5202203525859588
1113	8	-0.34736339862032395
1113	9	0.08891460743825519
1113	10	0.24780078389878213
1113	11	-0.24053493703433304
1113	12	-0.20975378924125465
1113	13	-0.6341381735018956
1113	14	-1.649299412262586
1113	15	-1.913475222506257
1113	16	-0.7077277216724127
1113	17	-0.46706156615550043
1113	18	-0.7927199709057788
1113	19	-0.32275588194391247
1113	20	0.007647913459756239
1113	21	-1.80870972235423
1113	22	-2.0804888864497855
1113	23	-0.8537470310121336
1113	24	-2.5585071787275666
1113	25	-0.8541450888544588
1114	1	-0.07449761113607778
1114	2	0.02072187258897884
1114	3	-0.1830983951578773
1114	4	0.08919105521752613
1114	5	-0.2309549734121461
1114	6	-0.09719100545881787
1114	7	-0.06408340564990311
1114	8	-0.2226222630009005
1114	9	0.14930614469442105
1114	10	-0.05222524570207554
1114	11	0.012451907605395718
1114	12	-0.13280164726539567
1114	13	-0.1875998501205416
1114	14	0.6279993398783503
1114	15	0.12122259985733308
1114	16	0.06614027701569825
1114	17	-0.678622509260002
1114	18	-0.39567792268854163
1114	19	-0.7912970269339894
1114	20	-1.8193230739471247
1114	21	-1.0000689360520525
1114	22	-1.6181659528096475
1114	23	-3.0810957599867237
1114	24	-2.2193420521456133
1114	25	-1.6209575039328854
1115	1	0.2832064669205252
1115	2	0.29219655247287335
1115	3	0.25407701997205123
1115	4	0.3785989818722584
1115	5	0.2015438058766037
1115	6	0.1434030570486442
1115	7	0.19958185476784251
1115	8	0.1803290878869248
1115	9	0.44464220542495525
1115	10	0.6228048266713202
1115	11	0.2986973404615796
1115	12	0.13604708399761734
1115	13	0.14045249577910113
1115	14	0.7950126089215388
1115	15	0.3522760256997586
1115	16	0.4465605482230828
1115	17	0.3172960372889406
1115	18	0.5782800088803626
1115	19	0.021255761240986715
1115	20	0.4687959597899294
1115	21	-0.4656349917039506
1115	22	0.5235084048926169
1115	23	0.664379042849319
1115	24	0.6470518356444899
1115	25	0.5157692043678352
1116	1	0.2523758529028477
1116	2	0.3780794362741382
1116	3	0.1412165186057983
1116	4	0.3881878003788586
1116	5	0.1731966798561544
1116	6	0.049565248638124615
1116	7	0.050878983698289915
1116	8	-0.013152480231887864
1116	9	0.12947984718815064
1116	10	0.10559067292705038
1116	11	0.03581597203036324
1116	12	-0.06409802750607696
1116	13	-0.461647052682558
1116	14	0.4279245161080212
1116	15	0.17716321341224983
1116	16	0.19000992557300123
1116	17	0.3460470842571013
1116	18	0.33879758800440385
1116	19	0.3785508835351834
1116	20	0.3012463966698107
1116	21	-0.23324990335014206
1116	22	-0.18586950172053207
1116	23	-1.1240653838820691
1116	24	-0.5676689071760279
1116	25	-1.2823953976432847
1201	1	0.6394854715164229
1201	2	0.8291903279003172
1201	3	0.7485001260601329
1201	4	0.6886535787586539
1201	5	0.6575656841941182
1201	6	0.5051109900817394
1201	7	0.5465290118393966
1201	8	0.5478518201050164
1201	9	0.9096396008633434
1201	10	1.163217633201846
1201	11	0.8142866637371284
1201	12	0.8483561432563391
1201	13	0.8747721447237566
1201	14	1.5422478832577085
1201	15	1.1235292484326393
1201	16	1.0107723221603209
1201	17	1.0232224699626684
1201	18	0.9239478646338029
1201	19	1.2269451788082577
1201	20	1.2658428814618574
1201	21	1.118163250381805
1201	22	1.2088834287952395
1201	23	0.9658611043433467
1201	24	1.2836239688134157
1201	25	1.3973171711613261
1202	1	0.4194192346707091
1202	2	0.7507888191849723
1202	3	0.49739331597636394
1202	4	0.5138812174831173
1202	5	0.46323115930458414
1202	6	0.42295475366253754
1202	7	0.43271004154222936
1202	8	0.451206106537299
1202	9	0.9460172546311583
1202	10	0.1567827224878684
1202	11	0.5416198871752371
1202	12	0.5484624034797605
1202	13	0.46414155750288444
1202	14	1.2277795936000402
1202	15	0.9842672601514693
1202	16	0.835536372129603
1202	17	-1.1584002520650571
1202	18	0.33737275682313594
1202	19	0.6562192363510917
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2210	19	0.5400746418073936
2210	20	0.7589644458197782
2210	21	0.6273540666103159
2210	22	0.8862909666606882
2210	23	1.2965383594587863
2210	24	1.0033191239576738
2210	25	0.8831613117571351
2211	1	-0.10286950992573907
2211	2	-0.8250652563736196
2211	3	-0.21780345594090988
2211	4	0.04843364955777929
2211	5	-0.024385534387931784
2211	6	-0.12095288930233039
2211	7	-0.07525050027015112
2211	8	0.04597517609420265
2211	9	0.4138389824912281
2211	10	0.4390706692411328
2211	11	0.1793730270398015
2211	12	0.08294635017169583
2211	13	-0.11278314981338156
2211	14	-1.1690871655513604
2211	15	-0.32876981638202807
2211	16	-0.10588107006741154
2211	17	0.011127088494959025
2211	18	-0.09034524527454835
2211	19	0.17792773835017073
2211	20	0.32926150870627424
2211	21	0.3299667022444055
2211	22	-0.37063543079158023
2211	23	0.2919941811498177
2211	24	-0.4269198001293546
2211	25	0.006271191771823226
2212	1	-0.020158466584064172
2212	2	-0.9647085398560762
2212	3	-0.28441647174915374
2212	4	0.08449017996439778
2212	5	0.10685765988205986
2212	6	0.059268061605457945
2212	7	0.07769462654788128
2212	8	0.11975651114156705
2212	9	-2.1046714616341973
2212	10	0.05675685999660374
2212	11	0.05839718006248518
2212	12	-0.022032833232330518
2212	13	0.025398544506820997
2212	14	0.6693671125906775
2212	15	0.26942770715336195
2212	16	0.3180444103148119
2212	17	0.3571510475867754
2212	18	0.10578211571048968
2212	19	-0.6232700488228318
2212	20	0.28233173021818203
2212	21	0.2400731547971091
2212	22	-1.811953688270222
2212	23	-0.3576604371367935
2212	24	0.2890670284002397
2212	25	-0.414652454288003
2213	1	-0.09837726177677553
2213	2	0.2178022555130248
2213	3	-0.05404617843397119
2213	4	0.142170241293627
2213	5	0.047281836759900386
2213	6	-0.0709670281213306
2213	7	-0.06321405576693451
2213	8	-0.09095258546214069
2213	9	0.3144140103261108
2213	10	0.34062458153204744
2213	11	-0.2797939751409402
2213	12	-0.17589590193259852
2213	13	-0.15075960439973102
2213	14	-2.0859203003312032
2213	15	-0.6965791200200968
2213	16	-0.3632976923596374
2213	17	-2.422359036571308
2213	18	-1.0821575998277169
2213	19	-0.678767715621035
2213	20	-0.03880686886572704
2213	21	-0.2860737436331391
2213	22	0.04821320887102587
2213	23	0.5248372703666533
2213	24	0.3540875449723586
2213	25	-1.2716453602690692
2214	1	0.0424673247926286
2214	2	0.0071186407289509646
2214	3	-0.04737370272913566
2214	4	0.18376453465928222
2214	5	0.09341604791134017
2214	6	0.040244619710350094
2214	7	0.07348719704659601
2214	8	-0.08267087125616968
2214	9	0.3752372763761471
2214	10	0.36137989142257254
2214	11	-0.28772925088028956
2214	12	-0.30972615480684595
2214	13	-0.2433922810916087
2214	14	0.5574837338966745
2214	15	0.16468911799849195
2214	16	0.13010453098225838
2214	17	0.03517321051449329
2214	18	0.05974044303062698
2214	19	-1.1598545827563314
2214	20	-0.16730070705986577
2214	21	0.2129073666956245
2214	22	0.3550341505956496
2214	23	0.7960332646868622
2214	24	0.5172221673014334
2214	25	0.43752584515250703
2215	1	-0.3132051743186999
2215	2	0.07979808855973758
2215	3	-0.024302386652394148
2215	4	0.2083617418762458
2215	5	0.033691346752043216
2215	6	-0.05663424093194891
2215	7	-0.03505819837304358
2215	8	-0.041475278577188135
2215	9	0.06978711836463702
2215	10	0.34669240760813125
2215	11	-0.03595607358442976
2215	12	-0.07459854948005784
2215	13	-0.1908480296916153
2215	14	0.5446789258953828
2215	15	0.06520664563411316
2215	16	0.05181804012988778
2215	17	0.22863449725105056
2215	18	0.033724349697742184
2215	19	-0.6876779395608992
2215	20	-1.8389760796877113
2215	21	-2.096841117577071
2215	22	-0.5736417732333763
2215	23	0.19899955433232464
2215	24	0.2517322470633374
2215	25	-0.4730932149864415
2216	1	-0.1685622624605152
2216	2	-0.3854557686718607
2216	3	-0.2045609374642332
2216	4	-0.009061468644055992
2216	5	0.008381389398167016
2216	6	-0.32604087211124977
2216	7	-0.16799877514024075
2216	8	-0.24448850138158207
2216	9	0.008700380444686573
2216	10	-2.0101783253424728
2216	11	-0.7636796732401407
2216	12	-0.5235716721175976
2216	13	-0.3769908259512391
2216	14	-0.885363051703564
2216	15	-0.7706917223370056
2216	16	-0.36574890258057735
2216	17	-0.002219455801999004
2216	18	-0.2657331727046994
2216	19	-1.6152047431074052
2216	20	-1.2175264179876066
2216	21	-1.0952153560889357
2216	22	-0.9952018803082865
2216	23	-4.775481157420568
2216	24	-2.24068281584735
2216	25	-1.4710646691049547
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	19.0
5	36.0
6	66.0
7	270.0
8	163.0
9	397.0
10	981.0
11	2371.0
12	4881.0
13	8731.0
14	14180.0
15	20928.0
16	28590.0
17	33184.0
18	33463.0
19	32205.0
20	30967.0
21	29378.0
22	27267.0
23	26837.0
24	26797.0
25	27606.0
26	29515.0
27	33373.0
28	38638.0
29	47743.0
30	59423.0
31	75369.0
32	102647.0
33	140873.0
34	196776.0
35	278887.0
36	410702.0
37	633497.0
38	993023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.80547233878475	18.59163861637385	11.95552145201562	20.647367592825777
2	13.184341190119591	23.7001839977434	38.68682380239185	24.428651009745153
3	20.304084400477336	31.358157882622617	29.997055001245283	18.340702715654764
4	12.638788880363572	20.452517263766747	32.87401876881308	34.034675087056605
5	9.819737873521968	45.43129155490915	32.55484775792377	12.194122813645121
6	27.886739744651923	33.80783275149472	23.453495398400122	14.851932105453233
7	23.064800531585302	38.78321375417957	19.723478862284708	18.428506851950424
8	22.111327922108583	37.221057502526186	23.69827818228178	16.969336393083452
9	27.558519031799495	22.02272796356396	17.472501949970383	32.946251054666156
10	14.792061690735473	29.128683866848192	28.637304907534123	27.44194953488221
11	28.12485431106469	29.8032793657257	20.213332861227418	21.858533461982198
12	25.31407289081602	28.818642175978777	23.99558487823747	21.871700054967736
13	28.100234111324152	22.72511600815111	29.8835994011188	19.291050479405936
14	28.005388926434954	25.701342509657866	23.28143122330991	23.011837340597268
15	30.50770778073285	24.979784708622397	23.530333561161143	20.98217394948361
16	23.396590408813385	26.750915011619853	28.98040382080054	20.87209075876623
17	20.014452402084828	33.24400659062582	26.24745651886543	20.494084488423916
18	20.446518440965512	25.074383838014235	35.50803947426274	18.97105824675752
19	22.415580208207015	33.06613344710572	23.320566395306535	21.197719949380726
20	21.3577411912993	30.269398526122426	28.727040552139076	19.6458197304392
21	22.57903483867313	31.908646397926727	24.418846511633397	21.093472251766745
22	20.828660276196487	29.941822467203032	21.991473275129245	27.23804398147124
23	24.676667383659122	25.793493780311742	23.625668565959028	25.90417027007011
24	19.63372343310221	30.42707382684598	21.527275559855575	28.411927180196233
25	23.488688568549602	31.557482106427038	23.00970546924928	21.94412385577408
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15415.0
1	15415.0
2	13553.5
3	11692.0
4	11692.0
5	11692.0
6	17248.0
7	22804.0
8	22804.0
9	22804.0
10	23150.0
11	23496.0
12	23496.0
13	23496.0
14	26620.5
15	29745.0
16	29745.0
17	29745.0
18	38289.5
19	46834.0
20	46834.0
21	46834.0
22	61333.0
23	75832.0
24	75832.0
25	75832.0
26	96081.5
27	116331.0
28	116331.0
29	116331.0
30	131817.0
31	147303.0
32	147303.0
33	147303.0
34	160710.0
35	174117.0
36	174117.0
37	174117.0
38	233433.5
39	292750.0
40	292750.0
41	292750.0
42	578939.5
43	865129.0
44	865129.0
45	865129.0
46	702839.0
47	540549.0
48	540549.0
49	540549.0
50	453444.0
51	366339.0
52	366339.0
53	366339.0
54	302712.5
55	239086.0
56	239086.0
57	239086.0
58	202244.0
59	165402.0
60	165402.0
61	165402.0
62	144901.0
63	124400.0
64	124400.0
65	124400.0
66	96328.5
67	68257.0
68	68257.0
69	68257.0
70	52819.0
71	37381.0
72	37381.0
73	37381.0
74	27693.5
75	18006.0
76	18006.0
77	18006.0
78	12175.5
79	6345.0
80	6345.0
81	6345.0
82	4045.0
83	1745.0
84	1745.0
85	1745.0
86	1194.5
87	644.0
88	644.0
89	644.0
90	404.5
91	165.0
92	165.0
93	165.0
94	90.5
95	16.0
96	16.0
97	16.0
98	11.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0801819818283675
2	0.018201722880096666
3	0.029293858703299824
4	0.035075929504756784
5	0.044280042209116854
6	0.01572369253661511
7	0.020089745998939756
8	0.03767196129316604
9	0.03764246093193411
10	0.02227277273010208
11	0.021210759725752842
12	0.015576190730455495
13	0.023688790069234397
14	0.04009099091418375
15	0.037317956958382956
16	0.02244977489749362
17	0.02162376478299977
18	0.027966342447863272
19	0.016697204457268577
20	0.014691179893497797
21	0.010797132210883922
22	0.020060245637707832
23	0.018644228298575514
24	0.019352236968141676
25	0.10788282102514345
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3389789.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.62350271608306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81245291915222	13.560229147045014
2	8.626721439754629	3.2131954032832457
3	4.065293925457579	2.2713003738730553
4	2.4797549112611015	1.8472688930036574
5	1.758235770504659	1.6372254323753954
6	1.2862697787173967	1.4372909230535393
7	1.0243882040649495	1.3354387550508924
8	0.8278604986962266	1.2334129796805642
9	0.7068568388410661	1.1847735232234633
>10	5.570760488386791	20.823488417184315
>50	0.540950302234413	6.912820958664903
>100	0.24590150309032782	8.323363790929687
>500	0.023357182687492872	2.9977243796930018
>1k	0.024477047610865818	9.574215934415415
>5k	0.003039633363440853	3.963258882229513
>10k+	0.003679556176796822	19.684992206294343
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	140869	4.155686386379801	No Hit
GCTTATGTACTCTGCGTTGATACCA	82753	2.4412433930253474	No Hit
CTTATGTACTCTGCGTTGATACCAC	52370	1.5449339177158226	No Hit
GAGTACATAAGCAGTGGTATCAACG	36210	1.0682080802079421	No Hit
TATGTACTCTGCGTTGATACCACTG	33948	1.0014782631013317	No Hit
CCCATGTACTCTGCGTTGATACCAC	29880	0.8814707936098678	No Hit
GCGTTGATACCACTGCTTATGTACT	27774	0.8193430328554373	No Hit
CATAAGCAGTGGTATCAACGCAGAG	22929	0.676413782686769	No Hit
CATGTACTCTGCGTTGATACCACTG	20746	0.6120144941174804	No Hit
ACTCTGCGTTGATACCACTGCTTAT	20100	0.592957260761658	No Hit
CTGCTTATGTACTCTGCGTTGATAC	19582	0.5776760736435218	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	18647	0.5500932358916735	No Hit
GTATCAACGCAGAGTACATAAGCAG	18008	0.5312425050644746	No Hit
GTACATGATAAGCAGTGGTATCAAC	16569	0.48879148525173693	No Hit
ATCATGTACTCTGCGTTGATACCAC	15549	0.45870111679517517	No Hit
TATCAACGCAGAGTACATAAGCAGT	15233	0.4493790026458874	No Hit
GTATCAACGCAGAGTACTTTTTTTT	14608	0.4309412768759353	No Hit
GTACATGGGAAGCAGTGGTATCAAC	14015	0.41344756266540483	No Hit
GGTATCAACGCAGAGTACATAAGCA	13990	0.4127100536346067	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	13470	0.3973698657940066	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	11845	0.34943177879213133	No Hit
GTGGTATCAACGCAGAGTACATAAG	10847	0.31999041828267183	No Hit
GTGGTATCAACGCAGAGTACATGGG	10761	0.3174533872167265	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9413	0.2776869002760939	No Hit
GCAGTGGTATCAACGCAGAGTACAT	9014	0.2659162561445565	No Hit
ACATAAGCAGTGGTATCAACGCAGA	8444	0.24910105024236023	No Hit
GTACTCTGCGTTGATACCACTGCTT	8411	0.24812753832170678	No Hit
GTACATGGGATAAGCAGTGGTATCA	8396	0.24768503290322788	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8336	0.2459150112293125	No Hit
CCACTGCTTATGTACTCTGCGTTGA	7395	0.21815517131007267	No Hit
GCTTATCCCATGTACTCTGCGTTGA	7263	0.2142611236274588	No Hit
GATACCACTGCTTATGTACTCTGCG	7053	0.20806604776875495	No Hit
GTACTGGTTCACTATCGGTCAGTCA	6878	0.20290348455316837	No Hit
GTTGATACCACTGCTTATGTACTCT	6871	0.2026969820245449	No Hit
ATACCACTGCTTATGTACTCTGCGT	6815	0.20104496179555717	No Hit
GAGTACATGATAAGCAGTGGTATCA	5980	0.17641216016690123	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5955	0.17567465113610317	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5842	0.17234111031689583	No Hit
CAGTGGTATCAACGCAGAGTACATA	5494	0.16207498460818653	No Hit
GGATACCACGTGTCCCGCCCTACTC	5336	0.15741392753354266	No Hit
GCTTATCATGTACTCTGCGTTGATA	5074	0.14968483289077875	No Hit
TACCACTGCTTATGTACTCTGCGTT	5052	0.14903582494367645	No Hit
GGATAAGCAGTGGTATCAACGCAGA	4943	0.1458202855693968	No Hit
TGATACCACTGCTTATGTACTCTGC	4884	0.14407976425671332	No Hit
GTATCAACGCAGAGTACATGGGAAG	4850	0.14307675197482791	No Hit
ATACAGGGTGACAGCCCCGTACACA	4813	0.14198523860924678	No Hit
GCAGAGTACATAAGCAGTGGTATCA	4609	0.13596716491793442	No Hit
GCGTTGATACCACTGCTTCCCATGT	4592	0.13546565877699174	No Hit
GGTATCAACGCAGAGTACATGGGAA	4546	0.13410864216032325	No Hit
GATAAGCAGTGGTATCAACGCAGAG	4525	0.13348913457445286	No Hit
GCGTTGATACCACTGCTTATCATGT	4508	0.13298762843351017	No Hit
GGGATAAGCAGTGGTATCAACGCAG	4338	0.1279725670240832	No Hit
GAGTACATGGGATAAGCAGTGGTAT	4154	0.12254450055740931	No Hit
CCCCATGTACTCTGCGTTGATACCA	4061	0.11980096696284047	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3856	0.1137533929102962	No Hit
TATCAACGCAGAGTACATGGGAAGC	3853	0.11366489182660042	No Hit
CAGTGGTATCAACGCAGAGTACATG	3851	0.11360589110413657	No Hit
ACGCAGAGTACATGATAAGCAGTGG	3657	0.10788282102514345	No Hit
ATCATTAACTGAATCCATAGGTTAA	3604	0.1063193018798515	No Hit
GTACACAAGCAGTGGTATCAACGCA	3486	0.10283825925448456	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3414	0.10071423324578609	No Hit
ATCAACGCAGAGTACATAAGCAGTG	3405	0.1004487299946988	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3399	0.10027172782730726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.900072246384657E-5	0.0
11	0.0	0.0	0.0	5.900072246384657E-5	0.0
12	0.0	0.0	0.0	5.900072246384657E-5	0.0
13	0.0	0.0	0.0	5.900072246384657E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	370	0.0	14.889602	14
TGTATCA	535	0.0	14.558509	14
CTTCGTT	435	0.0	13.538149	12
CAGTTGT	595	0.0	13.4102955	10
GTACAAA	835	0.0	13.319159	1
GCAGTTG	620	0.0	12.869558	9
GTTGTAT	640	0.0	12.615247	12
TTGTATC	625	0.0	12.614059	13
AGCAGTT	615	0.0	12.356735	8
TTCGTTG	470	0.0	12.32789	13
GTTATGG	85	3.9214938E-6	12.301303	1
GGTCGCT	70	1.09244924E-4	12.213308	7
GTAAGAC	125	1.4078978E-9	12.162617	3
ACTCTTC	510	0.0	12.106517	9
CCTACGA	55	0.0030661426	12.092798	2
GTCCTAG	150	1.4551915E-11	12.040367	1
AGTTGTA	665	0.0	11.998155	11
TAGTACC	135	3.7107384E-10	11.965714	4
CCTATAA	120	9.942596E-9	11.881941	1
GGTTATA	120	9.942596E-9	11.881941	1
>>END_MODULE