FastQCFastQC Report
Wed 25 May 2016
SRR1295328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3389789
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1419484.1875172761490465No Hit
GCTTATGTACTCTGCGTTGATACCA937682.7661898719949827No Hit
CTTATGTACTCTGCGTTGATACCAC617281.8209982981241606No Hit
GTATCAACGCAGAGTACTTTTTTTT401761.18520651285375No Hit
GAGTACATAAGCAGTGGTATCAACG375641.1081515693159663No Hit
CCCATGTACTCTGCGTTGATACCAC370301.0923983764181193No Hit
TATGTACTCTGCGTTGATACCACTG325640.9606497631563498No Hit
GCGTTGATACCACTGCTTATGTACT316580.9339224358802273No Hit
CATAAGCAGTGGTATCAACGCAGAG284060.8379872611540128No Hit
GGTATCAACGCAGAGTACTTTTTTT266610.7865091308043066No Hit
TATCAACGCAGAGTACTTTTTTTTT255610.7540587334491912No Hit
CATGTACTCTGCGTTGATACCACTG244460.7211658306755966No Hit
ACTCTGCGTTGATACCACTGCTTAT235820.6956775185712148No Hit
CTGCTTATGTACTCTGCGTTGATAC227630.6715167227222697No Hit
ACGCAGAGTACATAAGCAGTGGTAT224850.663315622299795No Hit
GTACTTTTTTTTTTTTTTTTTTTTT210400.620687600319666No Hit
GTATCAACGCAGAGTACATAAGCAG192270.5672034454061889No Hit
ATCATGTACTCTGCGTTGATACCAC176990.5221268934438101No Hit
TATCAACGCAGAGTACATAAGCAGT169570.5002376254097232No Hit
GTACATGATAAGCAGTGGTATCAAC155810.4596451283545967No Hit
GGTATCAACGCAGAGTACATAAGCA149250.44029289138645505No Hit
ACGCAGAGTACTTTTTTTTTTTTTT139720.4121790471324322No Hit
AAGCAGTGGTATCAACGCAGAGTAC135020.39831387735342816No Hit
GTACATGGGAAGCAGTGGTATCAAC129650.3824721833718854No Hit
GTGGTATCAACGCAGAGTACATAAG111120.3278080140091315No Hit
GTGGTATCAACGCAGAGTACATGGG109420.3227929525997046No Hit
ACATAAGCAGTGGTATCAACGCAGA106680.3147098536221576No Hit
GCAGTGGTATCAACGCAGAGTACAT100410.2962131271297417No Hit
CCACTGCTTATGTACTCTGCGTTGA87370.25774465608331376No Hit
GTACTCTGCGTTGATACCACTGCTT86770.2559746344093983No Hit
GATACCACTGCTTATGTACTCTGCG82590.2436434834144544No Hit
GCTTATCCCATGTACTCTGCGTTGA82100.24219796571409014No Hit
GTACATGGGATAAGCAGTGGTATCA79770.23532438154705207No Hit
GAGTACTTTTTTTTTTTTTTTTTTT78140.23051582266624854No Hit
ATACCACTGCTTATGTACTCTGCGT74660.22024969695753926No Hit
GTACTGGTTCACTATCGGTCAGTCA71340.21045557702854073No Hit
GTTGATACCACTGCTTATGTACTCT70170.20700403476440568No Hit
CAGTGGTATCAACGCAGAGTACATA65000.19175234800750135No Hit
GAGTACATGATAAGCAGTGGTATCA61940.18272523747053282No Hit
GAGTACATGGGAAGCAGTGGTATCA60490.17844768509190395No Hit
TACCACTGCTTATGTACTCTGCGTT58330.17207560706580852No Hit
GCTTATCATGTACTCTGCGTTGATA56790.16753255143609233No Hit
GGATACCACGTGTCCCGCCCTACTC56290.16605753337449616No Hit
TGATACCACTGCTTATGTACTCTGC56020.16526102362123424No Hit
GCAGAGTACTTTTTTTTTTTTTTTT55520.16378600555963807No Hit
GGATAAGCAGTGGTATCAACGCAGA55040.16236998822050577No Hit
ATACAGGGTGACAGCCCCGTACACA54560.16095397088137345No Hit
GCAGAGTACATAAGCAGTGGTATCA51640.15233986540165184No Hit
GCGTTGATACCACTGCTTATCATGT51010.15048134264404067No Hit
GCGTTGATACCACTGCTTCCCATGT50600.14927182783353182No Hit
GTATCAACGCAGAGTACATGGGAAG49670.14652829423896294No Hit
CCCCATGTACTCTGCGTTGATACCA49620.14638079243280333No Hit
GGGATAAGCAGTGGTATCAACGCAG49360.14561378304077333No Hit
GGTATCAACGCAGAGTACATGGGAA48930.14434526750780063No Hit
GATAAGCAGTGGTATCAACGCAGAG46620.13753068406322635No Hit
CAGTGGTATCAACGCAGAGTACATG44130.13018509411647747No Hit
ACGCAGAGTACATGATAAGCAGTGG43610.12865107533241743No Hit
GAGTACATGGGATAAGCAGTGGTAT43120.1272055576320532No Hit
ACGCAGAGTACATGGGAAGCAGTGG42560.12555353740306552No Hit
TATCAACGCAGAGTACATGGGAAGC41830.12340001103313511No Hit
ATCAACGCAGAGTACATAAGCAGTG41330.12192499297153894No Hit
ACTCTGCGTTGATACCACTGCTTCC40040.11811944637262083No Hit
ATCAACGCAGAGTACTTTTTTTTTT39400.11623142325377775No Hit
CATGTAAGCAGTGGTATCAACGCAG38710.11419589832877503No Hit
ATCATTAACTGAATCCATAGGTTAA38590.11384189399399194No Hit
TATCAACGCAGAGTACATGATAAGC37960.11198337123638079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA37170.10965284269905885No Hit
GTACACAAGCAGTGGTATCAACGCA36530.10776481958021575No Hit
GCGTTGATACCACTGCTTATCCCAT36470.10758781741282422No Hit
CCCATATTCAGACAGGATACCACGT35610.10505078634687882No Hit
GGTATCAACGCAGAGTACATGGGGG35510.10475578273455959No Hit
GCTTACATGTACTCTGCGTTGATAC35500.10472628237332766No Hit
GTGGTATCAACGCAGAGTACTTTTT34890.10292676033818035No Hit
ACCCTGTATCGCGCGCCTTTCCAGA34150.10074373360701802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC851.7013008E-814.540843
CCGCGCG500.001504650813.2947549
TAGGGCT905.359925E-712.676634
GTACAAA5600.011.8983981
CTAGGGC752.0600928E-411.4089673
TACGTCG752.0833798E-411.3951687
TCCTTAC855.2211795E-511.1984931
GGACTGT855.3467666E-511.1723926
ACAGGGT11700.011.1326823
TACCGCG1553.1650416E-1011.0275827
GTCTAGT951.3320152E-511.0216741
AATCCGT700.001496739710.8531816
CAGGGTG11800.010.7966214
CCCCGTA12500.010.71177415
GTAACCG803.7869296E-410.6832859
CCCGTAC12600.010.62644616
CCCTTAG909.333617E-510.5763541
AGGGTGA11950.010.5019785
AAGCGAC3900.010.47022914
CTTACAC2100.010.4129473
Produced by FastQC (version 0.11.5)