FastQCFastQC Report
Wed 25 May 2016
SRR1295327_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295327_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388688
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1043754.3695534954753406No Hit
GCTTATGTACTCTGCGTTGATACCA610282.5548753123053327No Hit
CTTATGTACTCTGCGTTGATACCAC385551.6140659642448072No Hit
GAGTACATAAGCAGTGGTATCAACG256821.0751508778040497No Hit
TATGTACTCTGCGTTGATACCACTG239891.0042751502079803No Hit
CCCATGTACTCTGCGTTGATACCAC237410.9938928817828029No Hit
GCGTTGATACCACTGCTTATGTACT201650.8441872693294393No Hit
CATAAGCAGTGGTATCAACGCAGAG175590.7350897228939066No Hit
CATGTACTCTGCGTTGATACCACTG159760.6688190337122303No Hit
ACTCTGCGTTGATACCACTGCTTAT148780.6228523775394693No Hit
GTACATGATAAGCAGTGGTATCAAC145200.6078650706998988No Hit
CTGCTTATGTACTCTGCGTTGATAC142530.596687386548599No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA139700.584839878627933No Hit
ATCATGTACTCTGCGTTGATACCAC136240.5703549396153872No Hit
ACGCAGAGTACATAAGCAGTGGTAT135620.5677593725090928No Hit
GTATCAACGCAGAGTACATAAGCAG129550.5423479332587596No Hit
TATCAACGCAGAGTACATAAGCAGT111250.4657368396374914No Hit
GTACATGGGAAGCAGTGGTATCAAC108670.45493593135646015No Hit
GGTATCAACGCAGAGTACATAAGCA101510.4249613176773191No Hit
GTATCAACGCAGAGTACTTTTTTTT98860.41386736149719006No Hit
AAGCAGTGGTATCAACGCAGAGTAC91030.3810878607838278No Hit
GTGGTATCAACGCAGAGTACATAAG76540.32042694567059404No Hit
GCAGTGGTATCAACGCAGAGTACAT71330.2986158091806046No Hit
GTGGTATCAACGCAGAGTACATGGG70590.29551787424728554No Hit
GGTATCAACGCAGAGTACTTTTTTT65890.27584180102215106No Hit
GTACTCTGCGTTGATACCACTGCTT65260.27320436993027136No Hit
GTACTTTTTTTTTTTTTTTTTTTTT64570.27031575492487925No Hit
ACATAAGCAGTGGTATCAACGCAGA64390.2695622031843422No Hit
TATCAACGCAGAGTACTTTTTTTTT64200.26876678745821975No Hit
GTACATGGGATAAGCAGTGGTATCA58960.2468300590114741No Hit
CCACTGCTTATGTACTCTGCGTTGA56860.23803862203854165No Hit
GTACTGGTTCACTATCGGTCAGTCA55620.232847487825953No Hit
GAGTACATGATAAGCAGTGGTATCA54320.22740516969985197No Hit
GTTGATACCACTGCTTATGTACTCT49720.2081477363305714No Hit
ATACCACTGCTTATGTACTCTGCGT49460.2070592727053512No Hit
GCTTATCCCATGTACTCTGCGTTGA48160.2016169545792502No Hit
GATACCACTGCTTATGTACTCTGCG48090.20132390668015243No Hit
GAGTACATGGGAAGCAGTGGTATCA46180.19332788543334248No Hit
GCTTATCATGTACTCTGCGTTGATA44700.1871320155667044No Hit
GATAAGCAGTGGTATCAACGCAGAG41410.17335876430911026No Hit
GCGTTGATACCACTGCTTATCATGT41040.17180979684245076No Hit
CAGTGGTATCAACGCAGAGTACATA39840.16678611857220366No Hit
TACCACTGCTTATGTACTCTGCGTT37990.15904128123890604No Hit
GGATACCACGTGTCCCGCCCTACTC37900.1586645053686375No Hit
ATACAGGGTGACAGCCCCGTACACA36700.15364082709839041No Hit
GCGTTGATACCACTGCTTCCCATGT36580.1531384592713657No Hit
GTATCAACGCAGAGTACATGGGAAG35720.14953815651102195No Hit
TGATACCACTGCTTATGTACTCTGC35380.1481147810011186No Hit
GAACAAAAAAAAAAAAAAAAAAAAA34280.14350974258672544No Hit
GGTATCAACGCAGAGTACATGGGAA33980.14225382301916367No Hit
GGATAAGCAGTGGTATCAACGCAGA33670.14095603946601648No Hit
ACGCAGAGTACATGATAAGCAGTGG32950.13794183250386824No Hit
CCCCATGTACTCTGCGTTGATACCA32910.13777437656152666No Hit
GCAGAGTACATAAGCAGTGGTATCA32440.13580676923901322No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30830.12906666755976504No Hit
GGGATAAGCAGTGGTATCAACGCAG30510.12772702002103248No Hit
CAGTGGTATCAACGCAGAGTACATG30400.12726651617959314No Hit
TATCAACGCAGAGTACATGATAAGC29710.12437790117420108No Hit
TATCAACGCAGAGTACATGGGAAGC29310.12270334175078536No Hit
GTATCAACGCAGAGTACATGATAAG27990.11717729565351356No Hit
GAGTACATGGGATAAGCAGTGGTAT27980.11713543166792817No Hit
ACGCAGAGTACATGGGAAGCAGTGG27940.11696797572558659No Hit
GTACACAAGCAGTGGTATCAACGCA27820.11646560789856189No Hit
ACCCTGTATCGCGCGCCTTTCCAGA25620.10725553106977555No Hit
GGTATCAACGCAGAGTACATGATAA25600.10717180309860476No Hit
ACTCTGCGTTGATACCACTGCTTCC25330.10604147548779917No Hit
GTTAATGATAGTGTGTCGAAACACA25080.10499487584816436No Hit
ATCATTAACTGAATCCATAGGTTAA24940.10440878004996887No Hit
ATCAACGCAGAGTACATAAGCAGTG24710.10344590838150482No Hit
ATGATAAGCAGTGGTATCAACGCAG24580.10290167656889472No Hit
GGTATCAACGCAGAGTACATGGGAT24370.10202253287160147No Hit
GTATCAACGCAGAGTACATGGGATA24230.10143643707340598No Hit
AAAAAGTACTCTGCGTTGATACCAC24050.10068288533286891No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACC350.002168838216.287763
TTAGGAC901.3460522E-1015.8353223
TAGGACG508.707727E-515.2019094
AGCAGTT4350.014.4113788
GTACAAA6800.014.1250831
TTAGGCA551.9587562E-413.8199184
TAGGACC551.9587562E-413.8199184
GTCGTTT551.9636331E-413.81586713
CGTCGTT551.9636331E-413.81586712
GCAGTTG4700.013.7423789
TTGCGGA500.00149145913.31003319
TAGGGCA655.448453E-513.15549854
GTTGTAT5050.012.78993712
AGTTGTA5150.012.72602411
AACAGTC604.0999678E-412.664287
ATACCGT604.0999678E-412.664286
TTGTATC5100.012.29205813
TGTATCA5200.012.2385914
GTCGCTT550.003073127212.08888412
ACCGTTC550.003073127212.0888848
Produced by FastQC (version 0.11.5)