##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295327_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2388688
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.491427930311534	31.0	30.0	33.0	23.0	34.0
2	29.257519190450992	31.0	29.0	34.0	20.0	34.0
3	29.816142585385784	31.0	30.0	34.0	25.0	34.0
4	32.834031485066276	35.0	33.0	37.0	22.0	37.0
5	33.49826013275907	35.0	33.0	37.0	27.0	37.0
6	33.40455471790372	36.0	35.0	37.0	26.0	37.0
7	33.481193023115615	36.0	35.0	37.0	27.0	37.0
8	33.37771613538478	36.0	33.0	37.0	26.0	37.0
9	34.22909563743779	38.0	34.0	39.0	22.0	39.0
10	34.16524217478381	38.0	33.0	39.0	23.0	39.0
11	34.61360546040337	38.0	34.0	39.0	25.0	39.0
12	34.61456833207183	38.0	34.0	39.0	25.0	39.0
13	34.337404466384896	38.0	34.0	39.0	24.0	39.0
14	34.48557618240641	38.0	32.0	40.0	18.0	41.0
15	35.01518281165225	38.0	33.0	40.0	23.0	41.0
16	34.98349303048368	38.0	33.0	40.0	23.0	41.0
17	35.0453868399724	38.0	33.0	40.0	24.0	41.0
18	34.98942850635998	38.0	33.0	40.0	23.0	41.0
19	35.062412922909985	39.0	33.0	40.0	23.0	41.0
20	35.10926416509816	39.0	33.0	41.0	23.0	41.0
21	35.10735726055475	39.0	33.0	41.0	23.0	41.0
22	34.87031793185213	39.0	33.0	40.0	22.0	41.0
23	34.5073266161173	38.0	32.0	40.0	19.0	41.0
24	34.84347139517593	38.0	32.0	40.0	23.0	41.0
25	33.590174187671224	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5569247630845275
1101	2	0.6813615222811436
1101	3	0.49961631908347925
1101	4	0.3966797368807633
1101	5	0.43880802270339814
1101	6	0.33039210385260276
1101	7	0.32402955386051957
1101	8	0.3006301214366971
1101	9	0.2142440383338453
1101	10	0.5190304290715986
1101	11	0.4129707392518682
1101	12	0.4948187128011696
1101	13	0.5823339376528907
1101	14	1.1515030013521041
1101	15	0.782764172813927
1101	16	0.566835973605663
1101	17	0.8011325944915413
1101	18	0.9202896916153094
1101	19	0.8550468879937583
1101	20	1.0370747193245364
1101	21	1.0510490332571507
1101	22	0.7098319082667501
1101	23	-1.7897368633208188
1101	24	0.06027450455735561
1101	25	0.688036637364668
1102	1	0.4412545742715075
1102	2	0.466587549974399
1102	3	0.4503931625368871
1102	4	0.4910293969720314
1102	5	0.23709957026855477
1102	6	0.22078820608150806
1102	7	0.11526687155691917
1102	8	0.31088689078197973
1102	9	0.33965518843098863
1102	10	0.8088281292424497
1102	11	0.5830501279361258
1102	12	0.41079515109566955
1102	13	0.5106246001172892
1102	14	1.072078497095184
1102	15	0.7612730946525943
1102	16	0.5680989176263864
1102	17	0.7529184065807186
1102	18	0.7928335659208372
1102	19	0.83669849523735
1102	20	0.8104066568331945
1102	21	0.7276274417443176
1102	22	0.5474575180445527
1102	23	0.6370940761962984
1102	24	0.7881827480344725
1102	25	0.5544264038436637
1103	1	-0.32618682160801526
1103	2	-1.4294619783321245
1103	3	-0.4346766242822149
1103	4	-0.11329250084122577
1103	5	-0.20137861846499305
1103	6	-0.25553195324516054
1103	7	-0.15904797210945532
1103	8	0.1116521111715727
1103	9	0.7039532300302369
1103	10	0.6890769662598828
1103	11	0.48879574591407504
1103	12	0.22194134811298483
1103	13	0.11330011085475178
1103	14	-1.2268302218980054
1103	15	-0.3463980875646939
1103	16	-0.29160118714369787
1103	17	0.20751849449604265
1103	18	0.29093018893578915
1103	19	0.2723576993260366
1103	20	0.37758311125647026
1103	21	0.4520545862651417
1103	22	0.03603776736133568
1103	23	0.7869962312700238
1103	24	0.5648742076404218
1103	25	0.4818854079560495
1104	1	0.10695140401860925
1104	2	0.2110502185426455
1104	3	-0.21160303352019483
1104	4	-0.10858879783640418
1104	5	-0.14573040492704337
1104	6	-0.0242814237885014
1104	7	-0.0588011800647692
1104	8	-0.022355125974023338
1104	9	-1.7467589820206157
1104	10	-0.3293560347386091
1104	11	-0.5163226094801203
1104	12	0.14415732949377258
1104	13	0.06827306891857177
1104	14	-1.6825877996021745
1104	15	-0.5619599981614911
1104	16	-0.11314030711546508
1104	17	0.17671746621593343
1104	18	0.3612450846644464
1104	19	0.2887008169515326
1104	20	0.3334158868619994
1104	21	0.4678846879890699
1104	22	0.6247439499296306
1104	23	1.0909600399212778
1104	24	0.7046098948460582
1104	25	0.5054425457143523
1105	1	-0.14189187953361326
1105	2	-0.0751817299008053
1105	3	-1.371310830639949
1105	4	-0.5421761197022334
1105	5	-0.37682002613752985
1105	6	-0.04738309364503124
1105	7	-0.06378065205999661
1105	8	-0.01546709785517919
1105	9	0.4568313576099996
1105	10	0.5056265030494913
1105	11	-0.07628695663033369
1105	12	-0.10633707963668826
1105	13	-0.22392108622335627
1105	14	0.508075140084955
1105	15	0.4160966093425742
1105	16	-0.08431833202443073
1105	17	0.268457515796932
1105	18	0.1839266688548804
1105	19	0.16967665161964618
1105	20	-0.22983630301864366
1105	21	0.04785145255299028
1105	22	0.21373631266335735
1105	23	0.4940711392921884
1105	24	0.2540041406009763
1105	25	0.6282733371611897
1106	1	-1.5064939022522204
1106	2	-1.0085886290199895
1106	3	-0.984747858030417
1106	4	-1.2614715714577969
1106	5	-0.8733428946548827
1106	6	-0.7248499440777394
1106	7	-0.762797318648083
1106	8	-0.6728846960553767
1106	9	-0.4793882585243239
1106	10	-1.9509182588412983
1106	11	-1.2036773863030632
1106	12	-1.0671012662824992
1106	13	-1.0479128045283872
1106	14	-0.33145182824508623
1106	15	-0.9958066595940664
1106	16	-1.3291486898111842
1106	17	-0.6262616900767384
1106	18	-0.6313882854297361
1106	19	-0.3930738461673542
1106	20	-0.253986852177718
1106	21	-0.327811543158262
1106	22	-0.35806724695679293
1106	23	-0.5465531344137489
1106	24	-0.7324700920788914
1106	25	-1.3690711876707127
1107	1	-0.05622327581871289
1107	2	0.075894229076102
1107	3	-0.16712064103007407
1107	4	-0.1139743538136031
1107	5	-0.1975022720092241
1107	6	-0.09327825378508692
1107	7	-0.24861002157609846
1107	8	-0.3109879613829989
1107	9	0.3302924164990273
1107	10	-0.12702127164557453
1107	11	-0.15733333076130407
1107	12	-0.3538582560369079
1107	13	-0.04874797116453067
1107	14	-1.166717466924304
1107	15	-0.7113008904388778
1107	16	-0.2811113899380331
1107	17	-0.07164214326417806
1107	18	0.2633341437445722
1107	19	0.2252501513274794
1107	20	0.2547308058103397
1107	21	0.2528725944452219
1107	22	-0.5406616150171075
1107	23	0.32045410431029353
1107	24	0.4068630121438517
1107	25	0.005165409986844338
1108	1	-0.02650591729221574
1108	2	0.27284726048371155
1108	3	0.01667490327041321
1108	4	-0.14030638116114602
1108	5	-0.12629378750696674
1108	6	-0.04317941666666769
1108	7	0.061463548483146724
1108	8	-0.07151592638997073
1108	9	0.5347762536813931
1108	10	0.457457676971913
1108	11	0.33108347055405574
1108	12	0.28363831557275176
1108	13	0.10664387431134514
1108	14	0.40760904089242445
1108	15	0.22563250144354896
1108	16	0.08663317686264094
1108	17	0.515625561085649
1108	18	0.495874222160559
1108	19	0.43425946375935354
1108	20	0.5719512019262041
1108	21	0.5900371983933042
1108	22	0.44927888375862324
1108	23	0.24358184791074677
1108	24	0.3658488054627185
1108	25	-0.535837143719867
1109	1	-0.6999294974450585
1109	2	-0.5420235492132832
1109	3	-0.563548400976746
1109	4	-0.44508549501940564
1109	5	-0.8908891137066206
1109	6	-1.148110633869024
1109	7	-0.6182044504465409
1109	8	-1.0853572142371064
1109	9	-0.7080897529972958
1109	10	-0.9957900526148933
1109	11	-1.0720588144958754
1109	12	-0.9956080216744851
1109	13	-1.0096981509050877
1109	14	-0.4887481364258903
1109	15	-1.2014068087107148
1109	16	-1.1457359812144858
1109	17	-1.1333950989392108
1109	18	-1.8896988746477987
1109	19	-1.9402542582974078
1109	20	-1.7035838311949973
1109	21	-1.4289422359185195
1109	22	-1.4675474613325363
1109	23	-1.0436429269987215
1109	24	-1.6703125708279671
1109	25	-0.894787642292151
1110	1	0.06658709779586047
1110	2	0.15052485154331663
1110	3	0.0724289742803208
1110	4	0.28111193915459154
1110	5	0.17385576200455688
1110	6	0.19519322380894266
1110	7	0.1361760708668669
1110	8	0.14980138644855856
1110	9	0.33980016482728814
1110	10	0.45716450364871264
1110	11	0.27255122040618573
1110	12	0.19196679199685462
1110	13	0.24175686577238054
1110	14	-2.490318614700488
1110	15	-0.6948674706867237
1110	16	-0.16736869987955316
1110	17	-0.20083240730303942
1110	18	-2.9046581708534944
1110	19	-0.463799828955608
1110	20	-1.898566953173443
1110	21	-0.2997799599440967
1110	22	-1.6165260129799677
1110	23	-0.5147590481308271
1110	24	0.1763051492896821
1110	25	0.17684352924481317
1111	1	-0.16088452243117857
1111	2	-1.1794375306931073
1111	3	-0.43882865746507704
1111	4	0.001135708254757617
1111	5	0.02563611421334855
1111	6	0.10760049435722863
1111	7	0.044459302665529776
1111	8	0.0175470504187345
1111	9	-1.6014708495207302
1111	10	-0.28332199723347173
1111	11	-0.015223006432258046
1111	12	-0.044471771204314337
1111	13	0.07622379210148722
1111	14	1.0000027009225363
1111	15	0.3555374695576319
1111	16	0.198773914721464
1111	17	-0.134553236891243
1111	18	0.24581687824933596
1111	19	-0.0835118033888449
1111	20	0.3639452476321807
1111	21	-0.4381451518612707
1111	22	-0.04820143493143547
1111	23	0.05478647602110698
1111	24	-0.4198158933548797
1111	25	-1.7127679757986272
1112	1	0.3689267983235389
1112	2	0.47400130071285673
1112	3	0.1421670820358365
1112	4	0.5681261728520397
1112	5	0.41329283971548136
1112	6	0.2655733083154388
1112	7	0.2697220334425623
1112	8	0.2602591624089925
1112	9	-0.22646577749800656
1112	10	0.7246195730647642
1112	11	0.3375544332106699
1112	12	0.2505186933929622
1112	13	0.29046765495399285
1112	14	0.9930739121215595
1112	15	0.47680669252374486
1112	16	0.5052198469592426
1112	17	-3.259284419686942
1112	18	-0.6991212430734493
1112	19	-0.910842296389454
1112	20	0.19118322693832823
1112	21	-0.244849960138815
1112	22	-1.481058403890657
1112	23	-1.2536022438003585
1112	24	-0.09188730743481699
1112	25	0.2649475851670857
1113	1	-0.35153128860033434
1113	2	-0.1989753255895934
1113	3	-0.23509378344806464
1113	4	-0.5495505379336549
1113	5	-0.21530755453966322
1113	6	-0.2625821408538087
1113	7	-0.3464217758130488
1113	8	-0.29773107508279395
1113	9	0.29582653452677476
1113	10	0.35504198928554587
1113	11	-0.1973446562476937
1113	12	-0.18795403729322402
1113	13	-0.6195727092626626
1113	14	-1.4433768529211477
1113	15	-1.8918716140565621
1113	16	-0.6767166967373726
1113	17	-0.4813739719257484
1113	18	-0.6457222005502175
1113	19	-0.2602471588695039
1113	20	0.20919223848436985
1113	21	-1.5349100371124393
1113	22	-1.9862062495679282
1113	23	-0.6481925000662017
1113	24	-2.675550125420358
1113	25	-0.8173803975607825
1114	1	-0.01209627273717473
1114	2	0.0350497753439285
1114	3	-0.09800566224258844
1114	4	0.21960643119865608
1114	5	-0.004752009133277113
1114	6	0.09437603034648845
1114	7	0.053463132570371386
1114	8	-0.045429851701534574
1114	9	0.3620431868831062
1114	10	0.2663456301997016
1114	11	0.12179282647333878
1114	12	0.21147263595010202
1114	13	0.08143814832212115
1114	14	0.9043018283359672
1114	15	0.37717635296269236
1114	16	0.2326924996308506
1114	17	-0.5829237505722986
1114	18	-0.26377209794208056
1114	19	-0.5540727067725868
1114	20	-1.654764903589836
1114	21	-0.7853344755079732
1114	22	-1.5244495495916794
1114	23	-3.033163300896806
1114	24	-1.9775432199233691
1114	25	-1.2987702658448228
1115	1	0.2846174460727582
1115	2	0.5352678287176111
1115	3	0.3741476569875495
1115	4	0.5381700618234646
1115	5	0.41106378756869333
1115	6	0.3087029200620819
1115	7	0.5393910400290025
1115	8	0.5101074390395084
1115	9	0.6426874562958815
1115	10	0.8182412488930169
1115	11	0.649648723311202
1115	12	0.5838204563801384
1115	13	0.4405374192287397
1115	14	1.0822506689184692
1115	15	0.48697333442935786
1115	16	0.6522930282596917
1115	17	0.5618704359561022
1115	18	0.7643690753537271
1115	19	0.22128220219916983
1115	20	0.8117828778074383
1115	21	-0.16074395805888742
1115	22	0.7837064898844162
1115	23	0.9030769891021322
1115	24	0.942097365471632
1115	25	0.9644865962105129
1116	1	0.4276343546259369
1116	2	0.6231006449931193
1116	3	0.48544221034867974
1116	4	0.7299069664129547
1116	5	0.6447876615387074
1116	6	0.5381234309965208
1116	7	0.44571964679318654
1116	8	0.4134270741516701
1116	9	0.7112537546700111
1116	10	0.7099740087622166
1116	11	0.5607973539337294
1116	12	0.49038273961603096
1116	13	0.07568936372188517
1116	14	1.150836976287117
1116	15	0.9109648811055777
1116	16	0.8838235353066679
1116	17	1.036598123068785
1116	18	0.9687085498603665
1116	19	1.2030149430121782
1116	20	0.9983102977524041
1116	21	0.41591955163308825
1116	22	0.3885607672357594
1116	23	-0.3676535202315847
1116	24	0.11412564866179054
1116	25	-0.5967254438684506
1201	1	0.4978506336030648
1201	2	0.7194066666859804
1201	3	0.6019933574835648
1201	4	0.53283434791053
1201	5	0.4642342558780115
1201	6	0.36874589172563077
1201	7	0.4171394569625946
1201	8	0.4086205553318294
1201	9	0.535209981830441
1201	10	0.9840289929581516
1201	11	0.6014913212797453
1201	12	0.6902116792771977
1201	13	0.671923001908084
1201	14	1.3080105761307763
1201	15	0.9061768758880859
1201	16	0.7858682657740133
1201	17	0.7536291401031718
1201	18	0.6921818994963616
1201	19	0.862934571253696
1201	20	1.0320788410968262
1201	21	0.9191912460327316
1201	22	0.9175042781579847
1201	23	0.513733371817132
1201	24	0.8023462254681135
1201	25	1.130898093991398
1202	1	0.41544853519220126
1202	2	0.7591673769440561
1202	3	0.618691055796571
1202	4	0.5077273818141776
1202	5	0.24073399124670658
1202	6	0.1938050711424566
1202	7	0.22627347415711796
1202	8	0.31801134597979797
1202	9	0.7944817283673302
1202	10	0.017706952311606017
1202	11	0.3731420335456832
1202	12	0.28083950533276436
1202	13	0.4089130669843115
1202	14	0.9309053753090808
1202	15	0.7254431767536573
1202	16	0.5406145543685525
1202	17	-1.4781650372772361
1202	18	-0.038759906972607894
1202	19	0.5423784640498113
1202	20	0.5837765131408617
1202	21	0.7181096041354209
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2210	19	0.6835952328226185
2210	20	0.8778674413487977
2210	21	0.9057559290152
2210	22	1.0665423931377092
2210	23	1.53883812257029
2210	24	1.1808082392102008
2210	25	1.0553238776732172
2211	1	0.06648116664226933
2211	2	-0.6636192742862868
2211	3	-0.16993708575304822
2211	4	0.17317485663515697
2211	5	0.13724114757654604
2211	6	0.017746555658440855
2211	7	0.1820476083845719
2211	8	0.2068562271808716
2211	9	0.6967222635122639
2211	10	0.752814804420872
2211	11	0.2969794667139283
2211	12	0.22062317785088226
2211	13	0.11844121798663565
2211	14	-1.0486141623511642
2211	15	-0.16506204652954182
2211	16	-0.004518835795515486
2211	17	0.053808408412372444
2211	18	0.13948632727522892
2211	19	0.4147805703793992
2211	20	0.5796058723653132
2211	21	0.5152219269964249
2211	22	-0.175330056951978
2211	23	0.25937664873947597
2211	24	-0.30287676757940574
2211	25	0.14034589441561707
2212	1	-0.08813760885093203
2212	2	-1.2519312251043644
2212	3	-0.4503385472832129
2212	4	0.02131120274566456
2212	5	0.011102064034140824
2212	6	0.10261655701486916
2212	7	0.08289153418019879
2212	8	0.13200988063245944
2212	9	-2.1390830226257336
2212	10	2.0031174862822354E-5
2212	11	0.05765964324250916
2212	12	-0.015751009223698986
2212	13	-0.05446537556079534
2212	14	0.6557870497875271
2212	15	0.3308964898682589
2212	16	0.3424997211730769
2212	17	0.37221763792622653
2212	18	0.09403272850819633
2212	19	-0.6195079536609782
2212	20	0.2246796164095599
2212	21	0.2411103240447403
2212	22	-1.806364716492567
2212	23	-0.26215279120310697
2212	24	0.2428248208360131
2212	25	-0.3427205179465105
2213	1	0.07515215612205495
2213	2	0.42108306812700036
2213	3	0.1348757778133809
2213	4	0.3443811028943031
2213	5	0.37751260345792303
2213	6	0.2639887743105618
2213	7	0.2240833146287855
2213	8	0.19954097019261496
2213	9	0.6141911556392472
2213	10	0.6492835932879473
2213	11	0.07903528814647842
2213	12	0.0684241583307923
2213	13	0.008194212623074293
2213	14	-1.620668818969662
2213	15	-0.3813058742380733
2213	16	-0.23337266802712975
2213	17	-1.9610436317772795
2213	18	-0.840608441784191
2213	19	-0.6336431691390914
2213	20	0.23063862347231634
2213	21	-0.10562409291594577
2213	22	0.4413224340643467
2213	23	0.9667882507898113
2213	24	0.743154872260618
2213	25	-0.8136737651598764
2214	1	0.22661362159257692
2214	2	0.14970776231165672
2214	3	0.04603657072021505
2214	4	0.3575293189068631
2214	5	0.35547845601632133
2214	6	0.2663640709232382
2214	7	0.2755991514733154
2214	8	0.13869425926993983
2214	9	0.5707219165624053
2214	10	0.49986593932008105
2214	11	-0.05715769203387566
2214	12	-0.1507933300647082
2214	13	0.110529193990196
2214	14	0.9615024255196047
2214	15	0.47720412824160263
2214	16	0.4445010822223239
2214	17	0.3868328780116457
2214	18	0.3287037395340704
2214	19	-0.929688556188772
2214	20	0.13663760260659785
2214	21	0.48226522244722503
2214	22	0.7092529798972151
2214	23	1.1256345414153301
2214	24	0.928814835225559
2214	25	1.0064292845409568
2215	1	-0.24446601136478208
2215	2	0.16899143016883755
2215	3	0.07452462351110256
2215	4	0.23226623334075924
2215	5	0.27624849819465425
2215	6	0.19674821941726606
2215	7	0.21043353515725016
2215	8	0.1393776469008472
2215	9	0.2883711054348126
2215	10	0.5453232289624097
2215	11	0.3453663360743988
2215	12	0.17158028305673412
2215	13	0.1006768978326491
2215	14	1.0397342515164212
2215	15	0.48498304624354915
2215	16	0.3169715521589893
2215	17	0.5815673576437064
2215	18	0.2852106602964568
2215	19	-0.40082445348295437
2215	20	-1.4809305880269363
2215	21	-1.7820455867285432
2215	22	-0.2228575003562412
2215	23	0.49180770014001496
2215	24	0.5034942903278576
2215	25	-0.08134102700911683
2216	1	0.14343037542631976
2216	2	-0.152070090925843
2216	3	0.005916412210414279
2216	4	0.434397807038053
2216	5	0.43106967738739854
2216	6	0.2014742587624596
2216	7	0.31717801298585613
2216	8	0.20859714926631057
2216	9	0.4154541066809543
2216	10	-1.439408404485512
2216	11	-0.15513492761135694
2216	12	0.09468768682162931
2216	13	0.2185409080022893
2216	14	-0.3817793843596249
2216	15	-0.24520174962683683
2216	16	0.11420500268777545
2216	17	0.5575666017380669
2216	18	0.3896785237802973
2216	19	-1.0529930705666217
2216	20	-0.6086813978184225
2216	21	-0.40222480854674814
2216	22	-0.35882001184771184
2216	23	-3.944372660549284
2216	24	-1.6857457838992644
2216	25	-0.6954154837144699
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	9.0
4	37.0
5	47.0
6	55.0
7	204.0
8	177.0
9	482.0
10	1256.0
11	2779.0
12	5502.0
13	9199.0
14	13666.0
15	19377.0
16	25321.0
17	29231.0
18	29929.0
19	28839.0
20	27559.0
21	26326.0
22	24976.0
23	24361.0
24	24887.0
25	25158.0
26	26614.0
27	29599.0
28	33782.0
29	40388.0
30	49271.0
31	60896.0
32	79959.0
33	104808.0
34	140943.0
35	193788.0
36	274299.0
37	402794.0
38	632168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.816522060095835	18.751469004169145	11.95372422146445	20.478284714270572
2	12.686011266088803	24.49471972746332	38.14914733888584	24.670121667562032
3	20.353024684507744	32.0495323365167	30.019535707630236	17.577907271345325
4	12.29267589907976	21.403565788167676	31.99137094507919	34.312387367673374
5	9.6105872813234	46.213891764926636	32.2756355484967	11.899885405253265
6	27.824255518708334	33.18833115599772	24.0746742370951	14.91273908819884
7	22.39001274201133	39.343545645753665	19.796806164403353	18.46963544783166
8	21.799898154212613	38.00535527931375	23.97103090383111	16.22371566264253
9	27.567117775845805	22.766440023250635	17.317784891444003	32.348657309459554
10	14.705801067956612	29.5136074019979	28.237327227533715	27.543264302511776
11	27.738583964370477	30.45656605246276	20.039386432575753	21.765463550591004
12	25.35009871223969	29.492956154179517	23.637509394934796	21.519435738646
13	28.210041535789916	22.995811246027635	30.273305413890185	18.520841804292267
14	28.36362631274114	26.65928763442549	22.978356653504182	21.998729399329186
15	31.44423733519494	24.776571183765466	23.366627622548208	20.412563858491385
16	23.282925101189143	26.46113943206781	29.668958863566132	20.586976603176915
17	19.999036893268933	34.13028321619024	26.020380175913537	19.850299714627287
18	20.491680056213063	25.08641962901221	35.87091932897749	18.550980985797242
19	22.245930908347876	34.14578385494304	22.928355849141298	20.679929387567793
20	20.861608599002544	30.960269683960444	28.82730939501574	19.350812322021273
21	22.020634164988742	32.77533747925886	24.54269001146808	20.66133834428432
22	20.29212282507384	30.763172241339028	21.295957444848554	27.64874748873858
23	24.31248458567767	26.1766492853826	23.61178979491257	25.89907633402716
24	19.23764908443191	31.128854505653525	21.183513912313817	28.449982497600747
25	23.17540663515928	32.089005229019484	22.791308185257407	21.94427995056383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15904.0
1	15904.0
2	13740.0
3	11576.0
4	11576.0
5	11576.0
6	17021.0
7	22466.0
8	22466.0
9	22466.0
10	21321.0
11	20176.0
12	20176.0
13	20176.0
14	21876.5
15	23577.0
16	23577.0
17	23577.0
18	29992.5
19	36408.0
20	36408.0
21	36408.0
22	46395.0
23	56382.0
24	56382.0
25	56382.0
26	68496.0
27	80610.0
28	80610.0
29	80610.0
30	90768.0
31	100926.0
32	100926.0
33	100926.0
34	109923.0
35	118920.0
36	118920.0
37	118920.0
38	161673.5
39	204427.0
40	204427.0
41	204427.0
42	413247.0
43	622067.0
44	622067.0
45	622067.0
46	503712.5
47	385358.0
48	385358.0
49	385358.0
50	313262.5
51	241167.0
52	241167.0
53	241167.0
54	195264.5
55	149362.0
56	149362.0
57	149362.0
58	130162.5
59	110963.0
60	110963.0
61	110963.0
62	99395.0
63	87827.0
64	87827.0
65	87827.0
66	67660.5
67	47494.0
68	47494.0
69	47494.0
70	39171.5
71	30849.0
72	30849.0
73	30849.0
74	22755.0
75	14661.0
76	14661.0
77	14661.0
78	9811.0
79	4961.0
80	4961.0
81	4961.0
82	3399.0
83	1837.0
84	1837.0
85	1837.0
86	1244.0
87	651.0
88	651.0
89	651.0
90	372.0
91	93.0
92	93.0
93	93.0
94	52.5
95	12.0
96	12.0
97	12.0
98	13.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07816006108792777
2	0.01921556938369515
3	0.03152358114580054
4	0.039394010435854325
5	0.047892399509689
6	0.018755065542255832
7	0.021978592432331055
8	0.031984084987239855
9	0.03332373252597241
10	0.02411365569718607
11	0.023276375985478222
12	0.018127105758474947
13	0.02624871896204109
14	0.035207611877315075
15	0.033114412598045456
16	0.024532295553039995
17	0.024406703596283816
18	0.02641617490438266
19	0.018420153657572692
20	0.01720609807559631
21	0.013354611401740202
22	0.018671337571085047
23	0.020387760980086142
24	0.018587609599914262
25	0.10805094679589801
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2388688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.061290667142572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29457274864063	16.4898475431886
2	7.3410847013181515	3.0922543741315014
3	3.0865772843427313	1.9502190405642545
4	1.9428261262839568	1.636737030455402
5	1.2748168834419953	1.3424644464776336
6	1.0173924483471524	1.2856558846317125
7	0.7889190444982002	1.1630957316314683
8	0.6333251712488861	1.0670916414792508
9	0.5396237035279605	1.022865450379411
>10	4.376681870400115	18.488846508510598
>50	0.43702754126944393	6.30269501725106
>100	0.21244413117250163	8.518627179673881
>500	0.023959859346708086	3.5329256120063173
>1k	0.024159524841263987	10.691333574524014
>5k	0.00279531692378261	4.030360259792049
>10k+	0.0037936443965621135	19.384980705302894
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	104375	4.3695534954753406	No Hit
GCTTATGTACTCTGCGTTGATACCA	61028	2.5548753123053327	No Hit
CTTATGTACTCTGCGTTGATACCAC	38555	1.6140659642448072	No Hit
GAGTACATAAGCAGTGGTATCAACG	25682	1.0751508778040497	No Hit
TATGTACTCTGCGTTGATACCACTG	23989	1.0042751502079803	No Hit
CCCATGTACTCTGCGTTGATACCAC	23741	0.9938928817828029	No Hit
GCGTTGATACCACTGCTTATGTACT	20165	0.8441872693294393	No Hit
CATAAGCAGTGGTATCAACGCAGAG	17559	0.7350897228939066	No Hit
CATGTACTCTGCGTTGATACCACTG	15976	0.6688190337122303	No Hit
ACTCTGCGTTGATACCACTGCTTAT	14878	0.6228523775394693	No Hit
GTACATGATAAGCAGTGGTATCAAC	14520	0.6078650706998988	No Hit
CTGCTTATGTACTCTGCGTTGATAC	14253	0.596687386548599	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	13970	0.584839878627933	No Hit
ATCATGTACTCTGCGTTGATACCAC	13624	0.5703549396153872	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	13562	0.5677593725090928	No Hit
GTATCAACGCAGAGTACATAAGCAG	12955	0.5423479332587596	No Hit
TATCAACGCAGAGTACATAAGCAGT	11125	0.4657368396374914	No Hit
GTACATGGGAAGCAGTGGTATCAAC	10867	0.45493593135646015	No Hit
GGTATCAACGCAGAGTACATAAGCA	10151	0.4249613176773191	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9886	0.41386736149719006	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	9103	0.3810878607838278	No Hit
GTGGTATCAACGCAGAGTACATAAG	7654	0.32042694567059404	No Hit
GCAGTGGTATCAACGCAGAGTACAT	7133	0.2986158091806046	No Hit
GTGGTATCAACGCAGAGTACATGGG	7059	0.29551787424728554	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6589	0.27584180102215106	No Hit
GTACTCTGCGTTGATACCACTGCTT	6526	0.27320436993027136	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6457	0.27031575492487925	No Hit
ACATAAGCAGTGGTATCAACGCAGA	6439	0.2695622031843422	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6420	0.26876678745821975	No Hit
GTACATGGGATAAGCAGTGGTATCA	5896	0.2468300590114741	No Hit
CCACTGCTTATGTACTCTGCGTTGA	5686	0.23803862203854165	No Hit
GTACTGGTTCACTATCGGTCAGTCA	5562	0.232847487825953	No Hit
GAGTACATGATAAGCAGTGGTATCA	5432	0.22740516969985197	No Hit
GTTGATACCACTGCTTATGTACTCT	4972	0.2081477363305714	No Hit
ATACCACTGCTTATGTACTCTGCGT	4946	0.2070592727053512	No Hit
GCTTATCCCATGTACTCTGCGTTGA	4816	0.2016169545792502	No Hit
GATACCACTGCTTATGTACTCTGCG	4809	0.20132390668015243	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4618	0.19332788543334248	No Hit
GCTTATCATGTACTCTGCGTTGATA	4470	0.1871320155667044	No Hit
GATAAGCAGTGGTATCAACGCAGAG	4141	0.17335876430911026	No Hit
GCGTTGATACCACTGCTTATCATGT	4104	0.17180979684245076	No Hit
CAGTGGTATCAACGCAGAGTACATA	3984	0.16678611857220366	No Hit
TACCACTGCTTATGTACTCTGCGTT	3799	0.15904128123890604	No Hit
GGATACCACGTGTCCCGCCCTACTC	3790	0.1586645053686375	No Hit
ATACAGGGTGACAGCCCCGTACACA	3670	0.15364082709839041	No Hit
GCGTTGATACCACTGCTTCCCATGT	3658	0.1531384592713657	No Hit
GTATCAACGCAGAGTACATGGGAAG	3572	0.14953815651102195	No Hit
TGATACCACTGCTTATGTACTCTGC	3538	0.1481147810011186	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3428	0.14350974258672544	No Hit
GGTATCAACGCAGAGTACATGGGAA	3398	0.14225382301916367	No Hit
GGATAAGCAGTGGTATCAACGCAGA	3367	0.14095603946601648	No Hit
ACGCAGAGTACATGATAAGCAGTGG	3295	0.13794183250386824	No Hit
CCCCATGTACTCTGCGTTGATACCA	3291	0.13777437656152666	No Hit
GCAGAGTACATAAGCAGTGGTATCA	3244	0.13580676923901322	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3083	0.12906666755976504	No Hit
GGGATAAGCAGTGGTATCAACGCAG	3051	0.12772702002103248	No Hit
CAGTGGTATCAACGCAGAGTACATG	3040	0.12726651617959314	No Hit
TATCAACGCAGAGTACATGATAAGC	2971	0.12437790117420108	No Hit
TATCAACGCAGAGTACATGGGAAGC	2931	0.12270334175078536	No Hit
GTATCAACGCAGAGTACATGATAAG	2799	0.11717729565351356	No Hit
GAGTACATGGGATAAGCAGTGGTAT	2798	0.11713543166792817	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2794	0.11696797572558659	No Hit
GTACACAAGCAGTGGTATCAACGCA	2782	0.11646560789856189	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	2562	0.10725553106977555	No Hit
GGTATCAACGCAGAGTACATGATAA	2560	0.10717180309860476	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2533	0.10604147548779917	No Hit
GTTAATGATAGTGTGTCGAAACACA	2508	0.10499487584816436	No Hit
ATCATTAACTGAATCCATAGGTTAA	2494	0.10440878004996887	No Hit
ATCAACGCAGAGTACATAAGCAGTG	2471	0.10344590838150482	No Hit
ATGATAAGCAGTGGTATCAACGCAG	2458	0.10290167656889472	No Hit
GGTATCAACGCAGAGTACATGGGAT	2437	0.10202253287160147	No Hit
GTATCAACGCAGAGTACATGGGATA	2423	0.10143643707340598	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2405	0.10068288533286891	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.372797117078497E-5	0.0
2	0.0	0.0	0.0	8.372797117078497E-5	0.0
3	0.0	0.0	0.0	8.372797117078497E-5	0.0
4	0.0	0.0	0.0	8.372797117078497E-5	0.0
5	0.0	0.0	0.0	8.372797117078497E-5	0.0
6	0.0	0.0	0.0	8.372797117078497E-5	0.0
7	0.0	0.0	0.0	8.372797117078497E-5	4.186398558539248E-5
8	0.0	0.0	0.0	1.2559195675617744E-4	4.186398558539248E-5
9	0.0	0.0	0.0	1.2559195675617744E-4	4.186398558539248E-5
10	0.0	0.0	0.0	2.0931992792696242E-4	4.186398558539248E-5
11	0.0	0.0	0.0	2.0931992792696242E-4	1.2559195675617744E-4
12	0.0	0.0	0.0	2.0931992792696242E-4	1.6745594234156993E-4
13	4.186398558539248E-5	0.0	0.0	2.511839135123549E-4	1.6745594234156993E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGACC	35	0.0021688382	16.28776	3
TTAGGAC	90	1.3460522E-10	15.835322	3
TAGGACG	50	8.707727E-5	15.201909	4
AGCAGTT	435	0.0	14.411378	8
GTACAAA	680	0.0	14.125083	1
TTAGGCA	55	1.9587562E-4	13.819918	4
TAGGACC	55	1.9587562E-4	13.819918	4
GTCGTTT	55	1.9636331E-4	13.815867	13
CGTCGTT	55	1.9636331E-4	13.815867	12
GCAGTTG	470	0.0	13.742378	9
TTGCGGA	50	0.001491459	13.310033	19
TAGGGCA	65	5.448453E-5	13.1554985	4
GTTGTAT	505	0.0	12.789937	12
AGTTGTA	515	0.0	12.726024	11
AACAGTC	60	4.0999678E-4	12.66428	7
ATACCGT	60	4.0999678E-4	12.66428	6
TTGTATC	510	0.0	12.292058	13
TGTATCA	520	0.0	12.23859	14
GTCGCTT	55	0.0030731272	12.088884	12
ACCGTTC	55	0.0030731272	12.088884	8
>>END_MODULE