FastQCFastQC Report
Wed 25 May 2016
SRR1295327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388688
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1076594.50703482413777No Hit
GCTTATGTACTCTGCGTTGATACCA712672.983520660714166No Hit
CTTATGTACTCTGCGTTGATACCAC458421.9191288272055622No Hit
CCCATGTACTCTGCGTTGATACCAC299761.254914831907725No Hit
GAGTACATAAGCAGTGGTATCAACG277541.1618930559369829No Hit
GTATCAACGCAGAGTACTTTTTTTT265411.1111120414219018No Hit
GCGTTGATACCACTGCTTATGTACT235410.9855200846657244No Hit
TATGTACTCTGCGTTGATACCACTG229220.9596062775883665No Hit
CATAAGCAGTGGTATCAACGCAGAG216260.9053505522696977No Hit
CATGTACTCTGCGTTGATACCACTG190300.796671645690019No Hit
ACTCTGCGTTGATACCACTGCTTAT180300.7548076601046265No Hit
GGTATCAACGCAGAGTACTTTTTTT180100.7539703803929186No Hit
TATCAACGCAGAGTACTTTTTTTTT171830.7193488643137991No Hit
CTGCTTATGTACTCTGCGTTGATAC170400.7133623143750879No Hit
ACGCAGAGTACATAAGCAGTGGTAT165110.6912162660004153No Hit
GTACTTTTTTTTTTTTTTTTTTTTT161360.6755172714058931No Hit
ATCATGTACTCTGCGTTGATACCAC158390.6630836676870315No Hit
GTACATGATAAGCAGTGGTATCAAC143670.6014598809053338No Hit
GTATCAACGCAGAGTACATAAGCAG140250.5871423978351296No Hit
TATCAACGCAGAGTACATAAGCAGT124330.5204949327831847No Hit
GGTATCAACGCAGAGTACATAAGCA111800.46803935884468795No Hit
GTACATGGGAAGCAGTGGTATCAAC106980.4478609177925288No Hit
AAGCAGTGGTATCAACGCAGAGTAC106210.4446373909024535No Hit
ACGCAGAGTACTTTTTTTTTTTTTT98550.4125695779440429No Hit
ACATAAGCAGTGGTATCAACGCAGA82170.34399636955517005No Hit
GCAGTGGTATCAACGCAGAGTACAT80520.33708881193358026No Hit
GTGGTATCAACGCAGAGTACATAAG79000.3307254861246006No Hit
GTGGTATCAACGCAGAGTACATGGG71600.29974613679141016No Hit
GTACTCTGCGTTGATACCACTGCTT68340.28609847749057227No Hit
CCACTGCTTATGTACTCTGCGTTGA67180.2812422551626667No Hit
GATACCACTGCTTATGTACTCTGCG59200.24783479466552352No Hit
GTACTGGTTCACTATCGGTCAGTCA58870.24645328314120557No Hit
GCTTATCCCATGTACTCTGCGTTGA56930.2383316699376394No Hit
ATACCACTGCTTATGTACTCTGCGT56400.2361128787016136No Hit
GAGTACATGATAAGCAGTGGTATCA55350.23171716021514738No Hit
GTACATGGGATAAGCAGTGGTATCA55210.2311310644169519No Hit
GAGTACTTTTTTTTTTTTTTTTTTT52590.2201627001935791No Hit
GCTTATCATGTACTCTGCGTTGATA51920.2173578131593578No Hit
CAGTGGTATCAACGCAGAGTACATA49800.20848264821525458No Hit
GTTGATACCACTGCTTATGTACTCT49050.2053428492963501No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA48740.20404506574320294No Hit
GAGTACATGGGAAGCAGTGGTATCA48650.20366828987293442No Hit
GATAAGCAGTGGTATCAACGCAGAG45220.1893089428171448No Hit
GCGTTGATACCACTGCTTATCATGT45020.18847166310543695No Hit
GCGTTGATACCACTGCTTCCCATGT42830.17930345026223599No Hit
GGATACCACGTGTCCCGCCCTACTC42690.1787173544640405No Hit
TACCACTGCTTATGTACTCTGCGTT42680.1786754904784551No Hit
CCCCATGTACTCTGCGTTGATACCA42630.17846617055052816No Hit
CTTATACACATCTCCGAGCCCACGA41490.17369367619379342No Hit
ATACAGGGTGACAGCCCCGTACACA41490.17369367619379342No Hit
TGATACCACTGCTTATGTACTCTGC40350.16892118183705868No Hit
GCAGAGTACATAAGCAGTGGTATCA40160.16812576611093621No Hit
ACGCAGAGTACATGATAAGCAGTGG40110.16791644618300924No Hit
GGATAAGCAGTGGTATCAACGCAGA39970.16733035038481375No Hit
GCAGAGTACTTTTTTTTTTTTTTTT38010.15912500921007683No Hit
GTATCAACGCAGAGTACATGGGAAG37300.15615266623351395No Hit
GGGATAAGCAGTGGTATCAACGCAG35230.14748682121733772No Hit
GGTATCAACGCAGAGTACATGGGAA35140.1471100453470692No Hit
CAGTGGTATCAACGCAGAGTACATG35100.14694258940472762No Hit
TATCAACGCAGAGTACATGGGAAGC34180.14309110273087153No Hit
ACGCAGAGTACATGGGAAGCAGTGG33140.13873724822999067No Hit
TATCAACGCAGAGTACATGATAAGC32790.13727200873450196No Hit
ACTCTGCGTTGATACCACTGCTTCC31890.13350425003181662No Hit
ATCAACGCAGAGTACATAAGCAGTG30670.12839684379039876No Hit
GTATCAACGCAGAGTACATGATAAG30660.12835497980481336No Hit
ACCCTGTATCGCGCGCCTTTCCAGA30120.12609432458320216No Hit
GTACACAAGCAGTGGTATCAACGCA29750.12454535711654265No Hit
ATGATAAGCAGTGGTATCAACGCAG29550.1237080774048348No Hit
GAGTACATGGGATAAGCAGTGGTAT29280.12257774979402918No Hit
CCCATATTCAGACAGGATACCACGT28490.11927049493278317No Hit
ATCAACGCAGAGTACTTTTTTTTTT28080.11755407152378208No Hit
AAAAAGTACTCTGCGTTGATACCAC26730.1119024334697541No Hit
CTGCTTATCATGTACTCTGCGTTGA26680.11169311354182715No Hit
ATCATTAACTGAATCCATAGGTTAA26620.1114419296283148No Hit
GTTAATGATAGTGTGTCGAAACACA25850.10821840273823957No Hit
GGTATCAACGCAGAGTACATGATAA25620.10725553106977555No Hit
GCGTTGATACCACTGCTTATCCCAT25540.10692061918509241No Hit
GTATCAACGCAGAGTACATGGGATA25450.10654384331482387No Hit
GGTATCAACGCAGAGTACATGGGAT25130.10520419577609132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAGT402.7686125E-416.61970715
TATACAC4900.015.9072153
CCGTGAC456.77088E-414.77585719
GGCTACC400.00521250814.27774051
GTTAGGT602.5553354E-514.2582963
TAGCAGC400.005291367414.2454626
AGGGACG400.005291367414.2454628
CTACTAG759.469004E-713.9604581
CCCCCGT500.001478135613.3258911
ACCGTTC655.4711996E-513.1496588
TAGGCTG1251.0186341E-1012.9275225
CTTATAC6850.012.7839621
ACCTAGA751.4500807E-512.6913251
AGTTAGG1051.9792424E-812.6740422
CCTTATA1207.348717E-1012.6740412
GGACAGT604.104466E-412.6626346
GGACCGT1301.9826984E-1012.4191226
CTAATAC701.08545966E-412.2213973
CGAACAC701.0946227E-412.21039618
CACGTCA701.0946227E-412.21039615
Produced by FastQC (version 0.11.5)