FastQCFastQC Report
Wed 25 May 2016
SRR1295326_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295326_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474829
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA925473.7395310948756455No Hit
GCTTATGTACTCTGCGTTGATACCA538562.1761503522061525No Hit
CTTATGTACTCTGCGTTGATACCAC350151.4148452276904788No Hit
GAGTACATAAGCAGTGGTATCAACG240360.9712186175287262No Hit
TATGTACTCTGCGTTGATACCACTG219350.8863238631840827No Hit
CCCATGTACTCTGCGTTGATACCAC179800.7265148420355507No Hit
GCGTTGATACCACTGCTTATGTACT177260.7162515066697538No Hit
CATAAGCAGTGGTATCAACGCAGAG157470.6362863858472646No Hit
ACTCTGCGTTGATACCACTGCTTAT135590.5478762371056748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA135190.5462599638197224No Hit
CTGCTTATGTACTCTGCGTTGATAC131790.5325216408891281No Hit
ACGCAGAGTACATAAGCAGTGGTAT128570.5195106409372122No Hit
CATGTACTCTGCGTTGATACCACTG122960.4968424081017314No Hit
GTACATGATAAGCAGTGGTATCAAC121570.4912258584330473No Hit
GTATCAACGCAGAGTACATAAGCAG116900.4723558678195544No Hit
ATCATGTACTCTGCGTTGATACCAC115410.4663352498293821No Hit
GTATCAACGCAGAGTACTTTTTTTT114480.46257741443954303No Hit
TATCAACGCAGAGTACATAAGCAGT103460.4180490854115577No Hit
GGTATCAACGCAGAGTACATAAGCA97180.3926735948221069No Hit
GTACATGGGAAGCAGTGGTATCAAC87420.3532365266448712No Hit
GGTATCAACGCAGAGTACTTTTTTT78210.3160218342358199No Hit
AAGCAGTGGTATCAACGCAGAGTAC76820.31040528456713573No Hit
GTGGTATCAACGCAGAGTACATGGG75500.3050715827234932No Hit
GTGGTATCAACGCAGAGTACATAAG71960.2907675641428155No Hit
TATCAACGCAGAGTACTTTTTTTTT71560.28915129085686325No Hit
GTACTTTTTTTTTTTTTTTTTTTTT67080.2710490300541977No Hit
GCAGTGGTATCAACGCAGAGTACAT58750.2373901388742414No Hit
ACATAAGCAGTGGTATCAACGCAGA58460.23621834074192602No Hit
GTACTCTGCGTTGATACCACTGCTT55830.22559134388678972No Hit
GTACTGGTTCACTATCGGTCAGTCA55690.22502564823670645No Hit
CCACTGCTTATGTACTCTGCGTTGA50140.20259985639411857No Hit
GTTGATACCACTGCTTATGTACTCT47860.19338709866419054No Hit
GATACCACTGCTTATGTACTCTGCG47610.19237692786047036No Hit
GAGTACATGATAAGCAGTGGTATCA44510.17985080989434019No Hit
ATACCACTGCTTATGTACTCTGCGT44040.17795168878334625No Hit
GTACATGGGATAAGCAGTGGTATCA38970.15746542488390108No Hit
GGATACCACGTGTCCCGCCCTACTC38870.157061356562413No Hit
ATACAGGGTGACAGCCCCGTACACA38050.1537479963262108No Hit
GAGTACATGGGAAGCAGTGGTATCA37500.15152562055802643No Hit
GCTTATCATGTACTCTGCGTTGATA37290.15067707708290148No Hit
CAGTGGTATCAACGCAGAGTACATA36920.1491820242933956No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35390.14299977897462815No Hit
GCTTATCCCATGTACTCTGCGTTGA34890.1409794373671878No Hit
TGATACCACTGCTTATGTACTCTGC33580.13568614235569407No Hit
GCGTTGATACCACTGCTTATCATGT32930.1330596982660216No Hit
TACCACTGCTTATGTACTCTGCGTT32650.131928306965855No Hit
GGTATCAACGCAGAGTACATGGGAA31110.12570565481493873No Hit
GATAAGCAGTGGTATCAACGCAGAG30960.12509955233270664No Hit
CCCCATGTACTCTGCGTTGATACCA30030.12134171694286755No Hit
GAACAAAAAAAAAAAAAAAAAAAAA29590.11956381632832006No Hit
GCAGAGTACATAAGCAGTGGTATCA29010.11722022006368925No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA28750.11616964242782027No Hit
GTATCAACGCAGAGTACATGGGAAG28680.1158867946027786No Hit
GCGTTGATACCACTGCTTCCCATGT28260.11418970765252873No Hit
GTACACAAGCAGTGGTATCAACGCA27620.11160367039500507No Hit
ACGCAGAGTACATGATAAGCAGTGG27610.11156326356285627No Hit
TATCAACGCAGAGTACATGATAAGC25780.10416881327962459No Hit
GTTAATGATAGTGTGTCGAAACACA24940.10077463937912477No Hit
TATCAACGCAGAGTACATGGGAAGC24920.10069382571482716No Hit
ACCCTGTATCGCGCGCCTTTCCAGA24800.10020894372904149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT651.0064468E-817.534311
CGTAATA704.4509943E-714.92503112
TAACCCT456.747486E-414.7826384
TCTAGAC851.7107595E-814.534193
GTACAAA9850.014.2851631
CTAACTG400.005268763314.2546874
CGGCCCA759.703072E-713.9303114
CACTAAT707.28301E-613.56848414
TAGTCTC707.28301E-613.5684847
AGGACCG1550.013.4883065
CGTTGCA852.7096758E-713.40831216
TCTATCC500.001496272913.3043743
CGCGTTT500.001502440713.29711214
ACGCGTA655.4661534E-513.15099118
GGACCGT802.0056468E-613.0596666
TCTTCGT2350.012.93310111
ACCTAAT2000.012.83492851
CAGTTGT3500.012.75411610
GTAATAA604.0669108E-412.6764731
CTAGAAC604.0822075E-412.6708323
Produced by FastQC (version 0.11.5)