FastQCFastQC Report
Wed 25 May 2016
SRR1295326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474829
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA955323.860145488839835No Hit
GCTTATGTACTCTGCGTTGATACCA627142.5340740713802856No Hit
CTTATGTACTCTGCGTTGATACCAC422051.7053703508404012No Hit
GTATCAACGCAGAGTACTTTTTTTT302321.2215793495227347No Hit
GAGTACATAAGCAGTGGTATCAACG254131.0268588253976336No Hit
CCCATGTACTCTGCGTTGATACCAC226130.9137196953809738No Hit
TATGTACTCTGCGTTGATACCACTG214880.8682620092135659No Hit
GGTATCAACGCAGAGTACTTTTTTT211620.8550893819330548No Hit
GCGTTGATACCACTGCTTATGTACT209150.8451088943922994No Hit
CATAAGCAGTGGTATCAACGCAGAG200040.8082982703047362No Hit
TATCAACGCAGAGTACTTTTTTTTT190370.7692248636168398No Hit
ACTCTGCGTTGATACCACTGCTTAT166980.6747132832207802No Hit
ACGCAGAGTACATAAGCAGTGGTAT155930.6300637336963483No Hit
CTGCTTATGTACTCTGCGTTGATAC154950.6261038641457652No Hit
GTACTTTTTTTTTTTTTTTTTTTTT148310.5992737275989574No Hit
CATGTACTCTGCGTTGATACCACTG144860.5853333705076189No Hit
ATCATGTACTCTGCGTTGATACCAC128400.5188237247906825No Hit
GTATCAACGCAGAGTACATAAGCAG124890.5046409267064512No Hit
GTACATGATAAGCAGTGGTATCAAC119340.4822151348638633No Hit
TATCAACGCAGAGTACATAAGCAGT116750.47174976533732227No Hit
ACGCAGAGTACTTTTTTTTTTTTTT110590.44685915673365717No Hit
GGTATCAACGCAGAGTACATAAGCA104810.4235040077516467No Hit
AAGCAGTGGTATCAACGCAGAGTAC87800.3547719862665259No Hit
GTACATGGGAAGCAGTGGTATCAAC80950.32709330624459304No Hit
GTGGTATCAACGCAGAGTACATGGG79090.31957763546491497No Hit
ACATAAGCAGTGGTATCAACGCAGA77140.3116983031958976No Hit
GTGGTATCAACGCAGAGTACATAAG73960.29884893057257694No Hit
GCAGTGGTATCAACGCAGAGTACAT68150.27537256109412No Hit
CCACTGCTTATGTACTCTGCGTTGA60150.24304709537507438No Hit
GAGTACTTTTTTTTTTTTTTTTTTT57010.230359350080349No Hit
GTACTGGTTCACTATCGGTCAGTCA56930.23003609542315853No Hit
GTACTCTGCGTTGATACCACTGCTT56630.22882389045869433No Hit
GATACCACTGCTTATGTACTCTGCG56160.2269247693477004No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA52740.21310563275280836No Hit
ATACCACTGCTTATGTACTCTGCGT49750.20102398994031506No Hit
GAGTACATGATAAGCAGTGGTATCA47870.19342750549633936No Hit
GTTGATACCACTGCTTATGTACTCT47570.19221530053187513No Hit
ATACAGGGTGACAGCCCCGTACACA45130.1823560334875662No Hit
GCTTATCATGTACTCTGCGTTGATA43900.17738599313326295No Hit
CAGTGGTATCAACGCAGAGTACATA43800.17698192481177488No Hit
GGATACCACGTGTCCCGCCCTACTC42400.17132496831094188No Hit
GCAGAGTACTTTTTTTTTTTTTTTT41620.16817323540333493No Hit
TACCACTGCTTATGTACTCTGCGTT39670.16029390313431757No Hit
GCGTTGATACCACTGCTTATCATGT38220.15443491247274055No Hit
GTACATGGGATAAGCAGTGGTATCA38180.15427328514414532No Hit
GCTTATCCCATGTACTCTGCGTTGA38060.15378840315835962No Hit
GAGTACATGGGAAGCAGTGGTATCA37960.15338433483687156No Hit
TGATACCACTGCTTATGTACTCTGC37570.15180846838306808No Hit
CTTATACACATCTCCGAGCCCACGA37550.15172765471877048No Hit
CCCCATGTACTCTGCGTTGATACCA37360.15095992490794313No Hit
GCAGAGTACATAAGCAGTGGTATCA35830.14477767958917565No Hit
GATAAGCAGTGGTATCAACGCAGAG35820.14473727275702686No Hit
ACGCAGAGTACATGATAAGCAGTGG34790.14057536904569973No Hit
GGTATCAACGCAGAGTACATGGGAA33140.13390824174114654No Hit
GCGTTGATACCACTGCTTCCCATGT31970.12918064237973614No Hit
GTATCAACGCAGAGTACATGGGAAG30870.12473589084336736No Hit
ATCAACGCAGAGTACTTTTTTTTTT29630.11972544365691529No Hit
GTACACAAGCAGTGGTATCAACGCA29050.11738184739228448No Hit
CAGTGGTATCAACGCAGAGTACATG28420.11483621696690963No Hit
CCCATATTCAGACAGGATACCACGT28350.11455336914186799No Hit
TATCAACGCAGAGTACATGATAAGC28290.11431092814897514No Hit
GGTATCAACGCAGAGTACATGGGGG27930.11285628219161808No Hit
ACCCTGTATCGCGCGCCTTTCCAGA27790.11229058654153479No Hit
ACGCAGAGTACATGGGAAGCAGTGG27710.11196733188434432No Hit
GTGGTATCAACGCAGAGTACTTTTT27710.11196733188434432No Hit
ATCAACGCAGAGTACATAAGCAGTG27490.11107838157707058No Hit
GGATAAGCAGTGGTATCAACGCAGA27490.11107838157707058No Hit
TATCAACGCAGAGTACATGGGAAGC26770.10816908966235646No Hit
ACTCTGCGTTGATACCACTGCTTCC26390.10663363004070181No Hit
GTTAATGATAGTGTGTCGAAACACA25900.10465369526541025No Hit
GTATCAACGCAGAGTACATGATAAG25300.10222928533648185No Hit
GGGATAAGCAGTGGTATCAACGCAG25300.10222928533648185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT552.8552677E-818.99187911
CCCTAGA555.7807483E-717.3101121
GCGTCCC402.7620126E-416.62494719
CCGCTCT402.7709684E-416.6178939
TATGTCC753.245077E-916.4816064
GATCATA350.00213956416.3209631
CCTAGAA851.0077201E-915.6612542
TATACAC5550.015.0767283
TTATACA5300.014.8908572
GTTATGC456.6543487E-414.8097611
TTGCGAA456.786295E-414.77146111
ACCGGAA801.2894998E-714.24995419
TACGGGA400.005295270614.24390812
GACAGTA602.5813282E-514.2430467
AGCGTAA759.726155E-713.92737710
CTAGACA500.001489769913.3120664
GGTTCGA655.480076E-513.14742717
CGTAATA802.0103926E-613.05691612
CTTATAC5700.013.0281361
TATACCG1352.7284841E-1112.6781595
Produced by FastQC (version 0.11.5)