FastQCFastQC Report
Wed 25 May 2016
SRR1295325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347909
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG48200.14397046036794908No Hit
GTATCAACGCAGAGTACTTTTTTTT36200.10812719222655096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2053.6379788E-1210.1974324
GAACCGT856.60701E-410.0571956
GGTATCA27050.09.9474981
CTACACT4300.09.5021524
TGGTATC12450.09.2347482
GTGGTAT13100.09.2177961
TTGGCGT1356.8329737E-69.150224
ACCGTTC1054.5021792E-49.0461538
AGAACCG950.00182084549.0020395
TACACCG950.00182084549.0020395
AACCGTT950.00182729378.9985427
GTCTTAG3200.08.9138631
TAGTACT3100.08.8891114
GTATAGG3000.08.8742461
TAGGACT3900.08.7712174
TATACTG8300.08.7007665
CTAGGAC3500.08.6876823
CCTGGAC5500.08.638323
TACACTG5350.08.5252955
CTATACT4900.08.3386244
Produced by FastQC (version 0.11.5)