FastQCFastQC Report
Wed 25 May 2016
SRR1295325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347909
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT123980.37032069868087814No Hit
GGTATCAACGCAGAGTACTTTTTTT104270.3114481307586317No Hit
TATCAACGCAGAGTACTTTTTTTTT75140.2244385973453878No Hit
GTGGTATCAACGCAGAGTACATGGG54110.16162326992758763No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49600.14815217498444552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC2850.012.6761362
AGCGTCA2950.012.2464373
TACCGTA701.0954379E-412.2097247
GGTATCA40450.011.5776661
GTACCGT1001.9399267E-611.3959146
GCAGCGT2900.010.8315441
TGGTATC16450.09.7671762
GTGGTAT17900.09.678161
TATAACG800.0044899849.5071022
GCGAAAC900.00111787969.49659411
GGAACGA1002.766915E-49.49659415
GCGTCAG3850.09.3836334
GTACTAG2151.0186341E-109.2972661
CTGATCG1054.5110317E-49.0443769
GTCATAG2950.09.0346311
TCTAGAC2651.8189894E-128.9689643
GAACCGT850.0074604028.9379726
GTATTGG3950.08.9161851
GTCCTAA2356.366463E-118.91105651
TCTGTCG2502.5465852E-118.7367368
Produced by FastQC (version 0.11.5)