FastQCFastQC Report
Wed 25 May 2016
SRR1295324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704939
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1192544.408750067931291No Hit
GCTTATGTACTCTGCGTTGATACCA695152.5699285640082827No Hit
CTTATGTACTCTGCGTTGATACCAC448571.6583368423465372No Hit
GAGTACATAAGCAGTGGTATCAACG308841.141763270816828No Hit
TATGTACTCTGCGTTGATACCACTG289301.0695250428937586No Hit
CCCATGTACTCTGCGTTGATACCAC270591.0003552760339511No Hit
GCGTTGATACCACTGCTTATGTACT235110.8691878079320827No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA208070.7692225222084491No Hit
CATAAGCAGTGGTATCAACGCAGAG198120.7324379588597006No Hit
CATGTACTCTGCGTTGATACCACTG172390.6373156658985656No Hit
CTGCTTATGTACTCTGCGTTGATAC170770.6313266214136437No Hit
ACTCTGCGTTGATACCACTGCTTAT170300.6295890591248083No Hit
ACGCAGAGTACATAAGCAGTGGTAT161100.5955772015561164No Hit
GTATCAACGCAGAGTACATAAGCAG150910.5579053723577501No Hit
GTACATGGGAAGCAGTGGTATCAAC143340.5299195286843807No Hit
GTATCAACGCAGAGTACTTTTTTTT131300.4854083585618752No Hit
TATCAACGCAGAGTACATAAGCAGT130110.4810089987241857No Hit
GTACATGATAAGCAGTGGTATCAAC128950.4767205471176984No Hit
GGTATCAACGCAGAGTACATAAGCA122310.452172858611599No Hit
ATCATGTACTCTGCGTTGATACCAC121360.44866076462352755No Hit
AAGCAGTGGTATCAACGCAGAGTAC103040.3809328047693497No Hit
GTGGTATCAACGCAGAGTACATGGG96430.35649602449445256No Hit
GTGGTATCAACGCAGAGTACATAAG93020.3438894555477961No Hit
GGTATCAACGCAGAGTACTTTTTTT88270.32632898560743884No Hit
TATCAACGCAGAGTACTTTTTTTTT80270.29675345728683716No Hit
GCAGTGGTATCAACGCAGAGTACAT77190.2853668788834055No Hit
GTACTCTGCGTTGATACCACTGCTT74580.2757178627688092No Hit
ACATAAGCAGTGGTATCAACGCAGA73190.2705791147231047No Hit
GTACATGGGATAAGCAGTGGTATCA69520.25701134110602863No Hit
GTACTGGTTCACTATCGGTCAGTCA69190.25579135056280383No Hit
CCACTGCTTATGTACTCTGCGTTGA63410.2344230313511691No Hit
GAGTACATGGGAAGCAGTGGTATCA63310.23405333724716157No Hit
GTACTTTTTTTTTTTTTTTTTTTTT62530.23116972323590293No Hit
GATACCACTGCTTATGTACTCTGCG62210.22998670210307884No Hit
GTTGATACCACTGCTTATGTACTCT61820.22854489509744952No Hit
GCTTATCCCATGTACTCTGCGTTGA60060.22203827886691715No Hit
ATACCACTGCTTATGTACTCTGCGT56580.2091729240474554No Hit
ATACAGGGTGACAGCCCCGTACACA53100.1963075692279937No Hit
GGATACCACGTGTCCCGCCCTACTC52610.19449606811835682No Hit
GCGTTGATACCACTGCTTCCCATGT47850.17689862876759882No Hit
GTATCAACGCAGAGTACATGGGAAG46470.17179685013229504No Hit
GAGTACATGATAAGCAGTGGTATCA46340.17131624779708526No Hit
CAGTGGTATCAACGCAGAGTACATA46160.1706507984098717No Hit
GGTATCAACGCAGAGTACATGGGAA44060.16288722222571378No Hit
TACCACTGCTTATGTACTCTGCGTT43330.1601884552664589No Hit
GGATAAGCAGTGGTATCAACGCAGA42660.15771150476960846No Hit
TGATACCACTGCTTATGTACTCTGC41830.15464304370634607No Hit
GTTAATGATAGTGTGTCGAAACACA40400.1493564180190385No Hit
ATCATTAACTGAATCCATAGGTTAA40260.14883884627342797No Hit
TATCAACGCAGAGTACATGGGAAGC40220.14869096863182496No Hit
GCTTATCATGTACTCTGCGTTGATA39010.14421766997333396No Hit
ACGCAGAGTACATGGGAAGCAGTGG38620.14277586296770464No Hit
CCCCATGTACTCTGCGTTGATACCA38000.14048375952285802No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37760.13959649367323995No Hit
GCAGAGTACATAAGCAGTGGTATCA37560.1388571054652249No Hit
GATAAGCAGTGGTATCAACGCAGAG37280.13782196197400384No Hit
ACCCTGTATCGCGCGCCTTTCCAGA36880.13634318555797378No Hit
GGGATAAGCAGTGGTATCAACGCAG36880.13634318555797378No Hit
GAGTACATGGGATAAGCAGTGGTAT34900.12902324229862486No Hit
ACTCTGCGTTGATACCACTGCTTCC34730.12839476232181207No Hit
GAACAAAAAAAAAAAAAAAAAAAAA34620.12798809880740378No Hit
GCGTTGATACCACTGCTTATCATGT34360.12702689413698423No Hit
CAGTGGTATCAACGCAGAGTACATG34040.12584387300416017No Hit
GTACACAAGCAGTGGTATCAACGCA30610.11316336523670219No Hit
ATCAACGCAGAGTACATAAGCAGTG30010.11094520061265707No Hit
GTATTTAGCCTTGGAGGATGGTCCC29940.1106864147398518No Hit
CCCATATTCAGACAGGATACCACGT28930.10695250428937585No Hit
ACGCAGAGTACATGATAAGCAGTGG27720.10247920563088483No Hit
GGTATCAACGCAGAGTACATGGGAT27410.10133315390846152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATC453.545212E-516.88624213
AGTCGTA350.002173032116.28316111
CTTCGTT2800.014.92623112
CGCGTGT456.724386E-414.7894119
CCTACAC902.2828317E-914.7812013
TCCGCGG400.00528593314.24776710
CGTATCA602.5734478E-514.24776714
GTCGGAT400.00528593314.24776711
CCCGATA400.00528593314.24776713
AGGTCAC1006.6211214E-1014.2475037
GTTGTAT4000.014.010303512
CGTCCTT903.650166E-813.7200729
GTACAAA6850.013.6001771
GTCCTAG1700.013.4205831
ATGTACG852.693905E-713.4148734
TAAGACT852.693905E-713.4148734
TCACGCG852.7066744E-713.40966410
TTGGACG500.001501820613.29791511
CACGTAG500.001501820613.29791512
GTATAAT1650.013.2511311
Produced by FastQC (version 0.11.5)