##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295324_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2704939
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.599004265900266	31.0	30.0	33.0	25.0	34.0
2	29.352802780395418	31.0	30.0	33.0	22.0	34.0
3	29.886978597299237	31.0	30.0	34.0	25.0	34.0
4	32.898117480652985	35.0	33.0	37.0	23.0	37.0
5	33.67473943035314	35.0	35.0	37.0	28.0	37.0
6	33.745918114974124	37.0	35.0	37.0	28.0	37.0
7	33.85876760991653	37.0	35.0	37.0	28.0	37.0
8	33.75652759637094	37.0	35.0	37.0	28.0	37.0
9	34.60872389358873	39.0	35.0	39.0	24.0	39.0
10	34.56917586681252	38.0	34.0	39.0	25.0	39.0
11	35.038934704257656	39.0	35.0	39.0	27.0	39.0
12	35.03252642665879	39.0	35.0	39.0	27.0	39.0
13	34.763714819446946	38.0	35.0	39.0	26.0	39.0
14	34.980799567014266	39.0	33.0	41.0	21.0	41.0
15	35.51792776103269	39.0	34.0	41.0	25.0	41.0
16	35.513506589242866	39.0	34.0	41.0	26.0	41.0
17	35.581379099491706	39.0	34.0	41.0	26.0	41.0
18	35.514986844435306	39.0	34.0	41.0	25.0	41.0
19	35.58393442513861	39.0	34.0	41.0	25.0	41.0
20	35.636109723731295	39.0	34.0	41.0	25.0	41.0
21	35.648312217022266	39.0	34.0	41.0	25.0	41.0
22	35.45488937088785	39.0	34.0	41.0	25.0	41.0
23	35.100914660182724	39.0	33.0	41.0	24.0	41.0
24	35.46223149579343	39.0	34.0	41.0	25.0	41.0
25	34.40473038393842	38.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5859727053468369
1101	2	0.6991558227718286
1101	3	0.5415435745780606
1101	4	0.454923977827697
1101	5	0.4348098644181846
1101	6	0.39948067852463964
1101	7	0.34328251002512644
1101	8	0.4941359471415936
1101	9	0.3530406226058673
1101	10	0.7080748075833725
1101	11	0.5276763986227095
1101	12	0.6206080904165887
1101	13	0.7066441845069278
1101	14	1.1919633079111875
1101	15	0.874220068929823
1101	16	0.718419564154317
1101	17	1.0121931478255703
1101	18	0.9542083093473437
1101	19	1.0100733344952104
1101	20	1.0157014328364795
1101	21	1.0821227362850863
1101	22	0.8108287941106909
1101	23	-1.416575969681091
1101	24	0.3306369189145286
1101	25	0.7506318975436059
1102	1	0.41403427747115273
1102	2	0.5282974555450544
1102	3	0.3407945358823632
1102	4	0.5812869497810738
1102	5	0.3056490237323004
1102	6	0.2700365420964559
1102	7	0.24914241919714186
1102	8	0.28817623790521196
1102	9	0.4589933001245896
1102	10	0.8839454754232037
1102	11	0.6093163182011025
1102	12	0.5951443954684308
1102	13	0.5832620038021261
1102	14	1.2268758559903787
1102	15	0.9349712437019804
1102	16	0.7477314120254093
1102	17	1.1153259388079775
1102	18	1.1084452617366054
1102	19	1.1368141040027737
1102	20	1.0618722781328174
1102	21	1.146063766595283
1102	22	0.9276505779526119
1102	23	1.079065204143248
1102	24	1.0297820130263133
1102	25	1.0404330970456002
1103	1	-0.2191734373306531
1103	2	-1.305924033166928
1103	3	-0.292994789778529
1103	4	0.05298012284045228
1103	5	-0.05443340791297402
1103	6	-0.0614560531765207
1103	7	0.11419075438386272
1103	8	0.22352976434394378
1103	9	0.7168116563163736
1103	10	0.8131919407471884
1103	11	0.45647798660348116
1103	12	0.5205128666879659
1103	13	0.19249305364355962
1103	14	-1.049004797579009
1103	15	-0.13719822069825938
1103	16	0.027056131516580706
1103	17	0.44009014664633384
1103	18	0.6004521575173527
1103	19	0.6284473802635162
1103	20	0.7065851206418898
1103	21	0.6624871392910521
1103	22	0.2775176047061265
1103	23	0.9862737073705006
1103	24	0.8379751654924945
1103	25	0.8108489032106192
1104	1	0.14912522976478115
1104	2	0.2634297321805299
1104	3	-0.13657457572177023
1104	4	0.006332557218762247
1104	5	0.021136310901184174
1104	6	0.08185366810901229
1104	7	0.06413872152946709
1104	8	0.09172548231545363
1104	9	-1.9226471126711502
1104	10	-0.29791003918107606
1104	11	-0.22666091208653683
1104	12	0.09897744340553061
1104	13	0.19657722329895222
1104	14	-1.529787478901227
1104	15	-0.4303167826560639
1104	16	-0.07191646153587783
1104	17	0.3785895459236812
1104	18	0.428879589178635
1104	19	0.5340929821846387
1104	20	0.5487212368024501
1104	21	0.4905867416379621
1104	22	0.7706458437327015
1104	23	1.1935762467822215
1104	24	0.8566097757522755
1104	25	0.8334267701166809
1105	1	-0.23016521417754632
1105	2	-0.06550687439386849
1105	3	-1.4795693164348478
1105	4	-0.507192140200182
1105	5	-0.39041858647939875
1105	6	-0.1248578611790947
1105	7	0.04873949835140934
1105	8	-0.024715609118729276
1105	9	0.44937133204959423
1105	10	0.5034348901711851
1105	11	-0.06297145877233845
1105	12	-0.059527371764311
1105	13	-0.13373758095620047
1105	14	0.615507954427386
1105	15	0.30686428529180176
1105	16	-0.21206112914277497
1105	17	0.22357100131165453
1105	18	0.12446510636630137
1105	19	0.20440427837869635
1105	20	-0.2816187020847991
1105	21	0.1105433374724285
1105	22	0.06457907708845312
1105	23	0.3524012318578613
1105	24	0.29219769637170856
1105	25	0.4363658176629883
1106	1	-1.3154624945242404
1106	2	-0.7828735167193948
1106	3	-0.9578299309046301
1106	4	-1.207123486299139
1106	5	-0.6351935598666572
1106	6	-0.39007753860843053
1106	7	-0.3524714906885933
1106	8	-0.2811780348688018
1106	9	-0.02974133808844215
1106	10	-1.3901578164113957
1106	11	-0.7423575093155961
1106	12	-0.4350834332367626
1106	13	-0.4271399433822509
1106	14	0.3186425573753837
1106	15	-0.4922943743907453
1106	16	-0.876716660336605
1106	17	-0.31247978512793395
1106	18	-0.23051655517544845
1106	19	-0.15181590169941472
1106	20	0.10483984282460312
1106	21	0.16996312284272364
1106	22	0.10301954887577125
1106	23	-0.24585239756192578
1106	24	-0.332343205272565
1106	25	-1.188814762631928
1107	1	0.07507030013229254
1107	2	0.14230725795290056
1107	3	0.03497148597163857
1107	4	0.13728835125618133
1107	5	-0.006091893402512483
1107	6	-0.02816687766117809
1107	7	-0.05291406737601534
1107	8	-0.0861538055246811
1107	9	0.36597197315727925
1107	10	0.018757879665635357
1107	11	-0.05687170908411332
1107	12	-0.0673813873187612
1107	13	0.127628984415054
1107	14	-1.044335707366983
1107	15	-0.41471401824322385
1107	16	-0.14108588550300283
1107	17	-0.013898776245213185
1107	18	0.2708202077956443
1107	19	0.2064369330030118
1107	20	0.29455673640725877
1107	21	0.4146998225117642
1107	22	-0.277108696922582
1107	23	0.5512106883449803
1107	24	0.38358163375706056
1107	25	-0.004017219869886901
1108	1	0.041750512011557106
1108	2	0.23336850814158794
1108	3	0.16827858980430221
1108	4	-0.10654418629591333
1108	5	-0.29409060545941657
1108	6	-0.06290661730611902
1108	7	-0.016508438766230427
1108	8	-0.0029556660399805423
1108	9	0.48977079690479286
1108	10	0.44208755693946244
1108	11	0.2953338360733895
1108	12	0.17962210746087948
1108	13	0.11928082883106583
1108	14	0.5303364669208719
1108	15	0.186487545425301
1108	16	0.100918851877708
1108	17	0.43867604814052896
1108	18	0.4564506886564317
1108	19	0.5431664414208512
1108	20	0.6134423212591216
1108	21	0.5778330253289425
1108	22	0.537605019843582
1108	23	0.39940811326231795
1108	24	0.3365953574680134
1108	25	-0.4754864679326616
1109	1	-0.6126758923673883
1109	2	-0.5244733520966456
1109	3	-0.61770008930948
1109	4	-0.4820676193216755
1109	5	-0.7378283573164381
1109	6	-0.9139972700733239
1109	7	-0.7433334048792659
1109	8	-0.9894758922583051
1109	9	-0.7296292951784693
1109	10	-0.803785550861221
1109	11	-0.9384062328210874
1109	12	-0.9056868626953545
1109	13	-0.9679244535541329
1109	14	-0.20162245133786172
1109	15	-0.9869107247808415
1109	16	-1.1518514114431326
1109	17	-0.9879595743659806
1109	18	-1.8075484627602378
1109	19	-1.9413805064287857
1109	20	-1.613532320411153
1109	21	-1.4076244008911374
1109	22	-1.3754126579786856
1109	23	-0.8888192656529199
1109	24	-1.8567423200229243
1109	25	-0.8971717525819827
1110	1	-0.12956408109716833
1110	2	-0.056571102588019784
1110	3	-0.26444866919241505
1110	4	-0.08599467498162738
1110	5	-0.04396044170270841
1110	6	-0.05180430964220761
1110	7	-0.08010397497797328
1110	8	-0.253847827937264
1110	9	0.14934453524983837
1110	10	-0.04947120281914863
1110	11	-0.08530127915037866
1110	12	-0.21689330073937185
1110	13	-0.10209143070266435
1110	14	-2.874949629030688
1110	15	-1.0156107237009024
1110	16	-0.4499298661613764
1110	17	-0.5159191415272559
1110	18	-3.117762371267979
1110	19	-0.7559458858106254
1110	20	-2.2494687067261907
1110	21	-0.6437225752577547
1110	22	-2.148786542943803
1110	23	-0.8436266049043013
1110	24	-0.13452538499193167
1110	25	-0.28076096347206203
1111	1	-0.26987221321385846
1111	2	-1.3647231556744153
1111	3	-0.39639016009658334
1111	4	-0.06976503829930181
1111	5	-0.13685186768269375
1111	6	-0.057500581254068095
1111	7	-0.18680367162254186
1111	8	-0.1522400682888545
1111	9	-1.7602224582931854
1111	10	-0.3820570219593762
1111	11	-0.3009838933235045
1111	12	-0.19812439136902782
1111	13	-0.12161168044329429
1111	14	0.7338241577086677
1111	15	0.10424266524491088
1111	16	0.07808187899428276
1111	17	-0.3410233746485787
1111	18	0.06957471413551275
1111	19	-0.18745852266694385
1111	20	0.20880280363912362
1111	21	-0.6359992352547863
1111	22	-0.18342955658594207
1111	23	-0.1625002105647937
1111	24	-0.6246446851962091
1111	25	-2.116289616303277
1112	1	-0.11600537612489603
1112	2	0.11617896731272381
1112	3	-0.14468481719558213
1112	4	0.15548762848079178
1112	5	-0.028820669346124816
1112	6	-0.14891260336512602
1112	7	-0.005973511070060056
1112	8	0.02579270750241136
1112	9	-0.4483153918203939
1112	10	0.2610278884712187
1112	11	0.004448650948880584
1112	12	-0.11534514495392045
1112	13	-0.1688619290949518
1112	14	0.351709348554472
1112	15	-0.10400108438872735
1112	16	-0.011195098697839967
1112	17	-3.51104472985849
1112	18	-1.0243869227382234
1112	19	-1.1781481086541916
1112	20	-0.1267384420353821
1112	21	-0.499895538816169
1112	22	-1.6891082028534044
1112	23	-1.5124031089352457
1112	24	-0.5267942174165512
1112	25	-0.13631938301631408
1113	1	-0.5069178149445293
1113	2	-0.16892787063256876
1113	3	-0.14524487823075205
1113	4	-0.6568364862188645
1113	5	-0.3496256894359888
1113	6	-0.4485601727820665
1113	7	-0.5493369923978335
1113	8	-0.5026051457069514
1113	9	-0.001056826569254099
1113	10	0.16244962248249095
1113	11	-0.3172589610237253
1113	12	-0.2956943020244438
1113	13	-0.5238638034448684
1113	14	-1.6843217286951386
1113	15	-1.8476478301476789
1113	16	-0.7263061869295626
1113	17	-0.4816951820784112
1113	18	-0.764557277386352
1113	19	-0.20314639270004164
1113	20	0.06957489647221138
1113	21	-1.8738898576879635
1113	22	-2.2328204188629925
1113	23	-0.8553707328403348
1113	24	-2.614034514355822
1113	25	-0.8001720168739368
1114	1	0.10093443061188978
1114	2	0.13056367432177396
1114	3	-0.08958489493918975
1114	4	0.30838187577808185
1114	5	0.048157561795541426
1114	6	0.1661446928302368
1114	7	0.051172903030391126
1114	8	0.04771822017902849
1114	9	0.29776854144849096
1114	10	0.1261339770470613
1114	11	0.0653210522277945
1114	12	0.08442985895270994
1114	13	0.05859809843627062
1114	14	0.8512629225882407
1114	15	0.3819851603746045
1114	16	0.1384959045059233
1114	17	-0.489923367484451
1114	18	-0.1558263984649102
1114	19	-0.49566648603683205
1114	20	-1.715008688928073
1114	21	-0.8278480289782593
1114	22	-1.351471932705067
1114	23	-2.940644981680826
1114	24	-2.051410281359537
1114	25	-1.5809637951702484
1115	1	0.24230758695164312
1115	2	0.33390766177639364
1115	3	0.20475881299486787
1115	4	0.3912875881783435
1115	5	0.3430932002433451
1115	6	0.12897458066642997
1115	7	0.21013603480984955
1115	8	0.21280639239321175
1115	9	0.44257871747266364
1115	10	0.6499917360111667
1115	11	0.2913391338564324
1115	12	0.31529414108370446
1115	13	0.16724772525944331
1115	14	0.800346262338536
1115	15	0.4049158938291839
1115	16	0.38162580950896796
1115	17	0.32677327248372023
1115	18	0.6136261250735728
1115	19	-0.037937200877109944
1115	20	0.6041907629259029
1115	21	-0.5068847168173747
1115	22	0.5716486486663044
1115	23	0.6428446154995058
1115	24	0.6746870016864719
1115	25	0.609876729675328
1116	1	0.30588058759110837
1116	2	0.4556300099801085
1116	3	0.3285690950169027
1116	4	0.5818263067282743
1116	5	0.23259644508716804
1116	6	0.16015677949983598
1116	7	0.1790867922091195
1116	8	0.030442611079799065
1116	9	0.2556189943327354
1116	10	0.23578607429158893
1116	11	0.16804504780395746
1116	12	0.0676832625930075
1116	13	-0.2769863804356234
1116	14	0.5817459275265691
1116	15	0.2151572658905394
1116	16	0.29092478456463056
1116	17	0.5415382749106143
1116	18	0.3981336422003068
1116	19	0.5135167144161201
1116	20	0.3843359655135572
1116	21	0.05190163931968783
1116	22	0.03214083620356689
1116	23	-0.9686143113338446
1116	24	-0.4147599275345115
1116	25	-1.1670711763557264
1201	1	0.6590600607792609
1201	2	0.8216697440939029
1201	3	0.717307623713765
1201	4	0.5879059733784047
1201	5	0.52059857812948
1201	6	0.3827690122494758
1201	7	0.5074765932905194
1201	8	0.49714854887432836
1201	9	0.8343545466914861
1201	10	1.02613901838604
1201	11	0.6790455934659434
1201	12	0.6656538960569662
1201	13	0.5812528675429505
1201	14	1.3990396920982207
1201	15	0.9013086126835361
1201	16	0.7329968119469399
1201	17	0.7642105304788416
1201	18	0.808323631783125
1201	19	0.9085258914586305
1201	20	1.0574182116567243
1201	21	1.035893652812426
1201	22	1.110570817849478
1201	23	0.5954580632879143
1201	24	1.082736724085109
1201	25	1.2866718168483047
1202	1	0.5151198794435707
1202	2	0.8331005269333538
1202	3	0.6595671327805483
1202	4	0.5894749693978625
1202	5	0.5104692105929871
1202	6	0.4034633952658382
1202	7	0.40951551412201326
1202	8	0.4542914060171839
1202	9	1.0704773905658769
1202	10	0.3265868290892655
1202	11	0.6701601445495342
1202	12	0.5842861384276006
1202	13	0.5820384712581159
1202	14	1.39895123566302
1202	15	1.0825807267923366
1202	16	0.961661672628054
1202	17	-1.1281352236122686
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2210	16	0.07078585075925758
2210	17	0.52240665770303
2210	18	0.3989142119747129
2210	19	0.7189044716019026
2210	20	0.7287552615341113
2210	21	0.8550664902307332
2210	22	0.9751929020938306
2210	23	1.370951884483091
2210	24	1.0816471887940509
2210	25	1.0873690010527852
2211	1	0.019009455757640126
2211	2	-0.6000210763083444
2211	3	-0.07371967589859807
2211	4	0.25376531616115017
2211	5	0.24969681903058927
2211	6	0.1488986412245339
2211	7	0.18038705951653355
2211	8	0.2644588242269137
2211	9	0.710891136655313
2211	10	0.7838961967584339
2211	11	0.34558713199999147
2211	12	0.19943224724050168
2211	13	0.06550916813323937
2211	14	-1.0675394909164027
2211	15	-0.06730655350594361
2211	16	0.07749847914381292
2211	17	0.1572006451207244
2211	18	0.14737916181034194
2211	19	0.43196290912031543
2211	20	0.5402236385887278
2211	21	0.635544130585096
2211	22	0.051309450865872464
2211	23	0.4663458014378534
2211	24	-0.1196367633608375
2211	25	0.18768350489985863
2212	1	-0.21243511314169794
2212	2	-1.126334717752755
2212	3	-0.561067444579809
2212	4	-0.039603132431103916
2212	5	-0.033030542249321115
2212	6	0.03108944568600691
2212	7	-0.027490170504776756
2212	8	-0.12041200475889013
2212	9	-2.438343049374808
2212	10	-0.26887782736154975
2212	11	-0.14536753711136186
2212	12	-0.23819285316903915
2212	13	-0.07075132112942839
2212	14	0.4142441175216405
2212	15	-0.04478271858581451
2212	16	-0.0027630678563710376
2212	17	0.18475096027305682
2212	18	-0.010111990143663263
2212	19	-0.6750854725916327
2212	20	0.12647940269660296
2212	21	0.11527036015170466
2212	22	-1.9206698486957734
2212	23	-0.576908536602815
2212	24	0.01044524712281003
2212	25	-0.7756952383329008
2213	1	0.015551325567436436
2213	2	0.25283330074610433
2213	3	0.01281510317004475
2213	4	0.20823070735650617
2213	5	0.17270108743748835
2213	6	-0.00885619979696628
2213	7	0.00954330255006397
2213	8	-0.0520247453910514
2213	9	0.3117604201025159
2213	10	0.2642341253455669
2213	11	-0.22623386722009542
2213	12	-0.21148496406949135
2213	13	-0.2500111495529538
2213	14	-2.195964273445611
2213	15	-0.9273341799405515
2213	16	-0.47260275624940107
2213	17	-2.52653543237723
2213	18	-1.2254447363955876
2213	19	-0.7973543491176898
2213	20	-0.07835286631027572
2213	21	-0.5288318434313268
2213	22	0.08518627025193837
2213	23	0.5381071567205353
2213	24	0.2638515926713936
2213	25	-1.4381777127941362
2214	1	0.012249115398162758
2214	2	0.05656087351811934
2214	3	0.105371390765292
2214	4	0.2786737917546347
2214	5	0.24378158438305775
2214	6	0.13171519259876874
2214	7	0.0719799252917781
2214	8	-0.02205420097175903
2214	9	0.2828247422912966
2214	10	0.38105250516063904
2214	11	-0.21480321900232724
2214	12	-0.28005090051274806
2214	13	-0.30783075110761615
2214	14	0.6236335875695076
2214	15	0.27102331345146524
2214	16	0.32133977586195783
2214	17	0.10497921290726708
2214	18	0.038275352867081835
2214	19	-1.0741635789853845
2214	20	-0.08552026782744093
2214	21	0.21027628752356264
2214	22	0.48228488137891645
2214	23	0.9334023211210862
2214	24	0.6147892260562315
2214	25	0.6017023826944765
2215	1	-0.4131315832575524
2215	2	0.08160914539780606
2215	3	0.009259356086253945
2215	4	0.17348528000675145
2215	5	0.011431796992738441
2215	6	0.09095443263642267
2215	7	-0.012531120231386694
2215	8	-0.06554468541597203
2215	9	0.07818077055268446
2215	10	0.43618006911565743
2215	11	0.033892326842249076
2215	12	-0.05234914708592697
2215	13	-0.09815250444957258
2215	14	0.648467253674319
2215	15	0.06060525571653841
2215	16	0.1627696328770014
2215	17	0.2981104026245802
2215	18	0.2451137500219076
2215	19	-0.5102886564550175
2215	20	-1.874790127400118
2215	21	-2.0944405117497524
2215	22	-0.4375612418721957
2215	23	0.18183645908668922
2215	24	0.3394550431713199
2215	25	-0.4322939900600531
2216	1	-0.012122977210907493
2216	2	-0.24313775605903487
2216	3	-0.11456048126390783
2216	4	0.25503091475159323
2216	5	0.21030401665800014
2216	6	-0.04469769791997891
2216	7	0.03811526863055548
2216	8	-0.1472819044383371
2216	9	0.2062110282673757
2216	10	-1.7139479532079989
2216	11	-0.6021522286756138
2216	12	-0.3461038885634409
2216	13	-0.16907330142461063
2216	14	-0.5219400383204231
2216	15	-0.47288998435147533
2216	16	-0.17751788044075312
2216	17	0.22726544595428066
2216	18	0.03532418236531498
2216	19	-1.5017432820563243
2216	20	-1.0807221460177843
2216	21	-1.0195559893091541
2216	22	-0.9224064043614106
2216	23	-4.511624768743733
2216	24	-2.0832624806295073
2216	25	-1.3709110253793142
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	18.0
5	28.0
6	41.0
7	173.0
8	120.0
9	326.0
10	859.0
11	2170.0
12	4565.0
13	8164.0
14	13352.0
15	19827.0
16	27363.0
17	31418.0
18	31661.0
19	29831.0
20	28344.0
21	26566.0
22	24178.0
23	23568.0
24	23411.0
25	23236.0
26	24967.0
27	27669.0
28	31764.0
29	38864.0
30	48091.0
31	61019.0
32	82619.0
33	113028.0
34	157645.0
35	223845.0
36	328433.0
37	503687.0
38	744080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.56659830265339	18.679137703228154	11.737347508351867	20.016916485766583
2	13.153732978510597	24.631043144093308	37.79501850469356	24.420205372702537
3	20.438817011287163	32.23704890364161	29.31308137836365	18.01105270670758
4	12.123670895859922	21.870366788548594	31.409147172892727	34.59681514269875
5	9.806696960573785	46.45767667973254	31.56542026693454	12.170206092759125
6	27.922167237059668	33.48553262480367	23.745788606535314	14.846511531601351
7	22.700743961781367	40.14054665660913	18.857249559982844	18.301459821626658
8	21.73311035453481	37.78144478131239	23.733891421356702	16.7515534427961
9	27.303896768619936	23.320995982224744	16.680239232184807	32.69486801697051
10	14.60128724608764	30.260895481326944	27.544411321198442	27.593405951386973
11	27.275604712870464	31.070017127491845	19.4116011394663	22.242777020171392
12	25.51849699099443	30.08073807527173	23.152741234394806	21.24802369933904
13	27.778919973879045	23.41438323998962	29.812945256184314	18.993751529947026
14	27.909712627184586	27.270794069699665	22.67546961031737	22.14402369279837
15	31.061873821885555	25.31557842206536	23.11905719039513	20.50349056565396
16	22.854961211072407	27.495914035749415	28.93250948461385	20.71661526856433
17	19.487572386104087	34.545435048759046	25.686792108181383	20.280200456955484
18	20.04949277669491	25.38146675970599	35.872685526727906	18.69635493687119
19	22.00110777021681	34.17622716240739	22.671870770987365	21.150794296388433
20	20.87861583712164	30.71080882719964	28.66003967322279	19.75053566245593
21	22.293813861245567	32.70213392134853	24.036452209715563	20.96760000769034
22	20.280871975107555	30.884898324418963	21.348525662476636	27.485704037996843
23	24.515050525854175	26.53734187676121	23.677285310877565	25.270322286507053
24	19.4996755341669	30.882158816319063	21.036478833919713	28.581686815594328
25	23.295831163625746	32.31504191190376	22.712887637459534	21.676239287010958
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22801.0
1	22801.0
2	18413.5
3	14026.0
4	14026.0
5	14026.0
6	19078.0
7	24130.0
8	24130.0
9	24130.0
10	23209.0
11	22288.0
12	22288.0
13	22288.0
14	24227.5
15	26167.0
16	26167.0
17	26167.0
18	32494.0
19	38821.0
20	38821.0
21	38821.0
22	50453.5
23	62086.0
24	62086.0
25	62086.0
26	77806.0
27	93526.0
28	93526.0
29	93526.0
30	102239.5
31	110953.0
32	110953.0
33	110953.0
34	121213.5
35	131474.0
36	131474.0
37	131474.0
38	175840.0
39	220206.0
40	220206.0
41	220206.0
42	455939.0
43	691672.0
44	691672.0
45	691672.0
46	567630.0
47	443588.0
48	443588.0
49	443588.0
50	368101.0
51	292614.0
52	292614.0
53	292614.0
54	233123.5
55	173633.0
56	173633.0
57	173633.0
58	148749.5
59	123866.0
60	123866.0
61	123866.0
62	111179.5
63	98493.0
64	98493.0
65	98493.0
66	76770.5
67	55048.0
68	55048.0
69	55048.0
70	44820.0
71	34592.0
72	34592.0
73	34592.0
74	25689.5
75	16787.0
76	16787.0
77	16787.0
78	11464.0
79	6141.0
80	6141.0
81	6141.0
82	3847.5
83	1554.0
84	1554.0
85	1554.0
86	952.0
87	350.0
88	350.0
89	350.0
90	222.5
91	95.0
92	95.0
93	95.0
94	57.5
95	20.0
96	20.0
97	20.0
98	14.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08318117340169223
2	0.01811501109636853
3	0.029760375372605445
4	0.035342756343119015
5	0.04454813953290628
6	0.01371565125867903
7	0.0184477357899753
8	0.03493609282871074
9	0.03530578693271826
10	0.020739839234821932
11	0.019039246356387336
12	0.014455039466694072
13	0.022070738009249007
14	0.03678456334874834
15	0.03460336813510397
16	0.020665900414020427
17	0.01915015458758959
18	0.02610040374293099
19	0.015046550033106107
20	0.012717477177858724
21	0.00913144436898577
22	0.01863258284197906
23	0.017819255813162516
24	0.01826288873797154
25	0.10950339360702774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2704939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.450977328435105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76254374051838	13.221304331603593
2	8.336927758159081	2.909750745928709
3	3.4906056578066105	1.8274344059067142
4	2.0726811320647363	1.4468124577894765
5	1.4204377108404795	1.2394013144165736
6	1.0673869776667748	1.1176167568757853
7	0.8325897439418478	1.0170653321791743
8	0.6692412876752709	0.9343131630779093
9	0.5615463501690177	0.8819579363098509
>10	4.823993689175972	17.181699405921
>50	0.5568481588989601	6.705329158990875
>100	0.33435753483107317	10.896415607157337
>500	0.029232275242455134	3.470459960723451
>1k	0.03328638640746716	11.794805118975985
>5k	0.003840736893169287	4.681631943539825
>10k+	0.004480859708697503	20.67400236060377
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	119254	4.408750067931291	No Hit
GCTTATGTACTCTGCGTTGATACCA	69515	2.5699285640082827	No Hit
CTTATGTACTCTGCGTTGATACCAC	44857	1.6583368423465372	No Hit
GAGTACATAAGCAGTGGTATCAACG	30884	1.141763270816828	No Hit
TATGTACTCTGCGTTGATACCACTG	28930	1.0695250428937586	No Hit
CCCATGTACTCTGCGTTGATACCAC	27059	1.0003552760339511	No Hit
GCGTTGATACCACTGCTTATGTACT	23511	0.8691878079320827	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	20807	0.7692225222084491	No Hit
CATAAGCAGTGGTATCAACGCAGAG	19812	0.7324379588597006	No Hit
CATGTACTCTGCGTTGATACCACTG	17239	0.6373156658985656	No Hit
CTGCTTATGTACTCTGCGTTGATAC	17077	0.6313266214136437	No Hit
ACTCTGCGTTGATACCACTGCTTAT	17030	0.6295890591248083	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	16110	0.5955772015561164	No Hit
GTATCAACGCAGAGTACATAAGCAG	15091	0.5579053723577501	No Hit
GTACATGGGAAGCAGTGGTATCAAC	14334	0.5299195286843807	No Hit
GTATCAACGCAGAGTACTTTTTTTT	13130	0.4854083585618752	No Hit
TATCAACGCAGAGTACATAAGCAGT	13011	0.4810089987241857	No Hit
GTACATGATAAGCAGTGGTATCAAC	12895	0.4767205471176984	No Hit
GGTATCAACGCAGAGTACATAAGCA	12231	0.452172858611599	No Hit
ATCATGTACTCTGCGTTGATACCAC	12136	0.44866076462352755	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	10304	0.3809328047693497	No Hit
GTGGTATCAACGCAGAGTACATGGG	9643	0.35649602449445256	No Hit
GTGGTATCAACGCAGAGTACATAAG	9302	0.3438894555477961	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8827	0.32632898560743884	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8027	0.29675345728683716	No Hit
GCAGTGGTATCAACGCAGAGTACAT	7719	0.2853668788834055	No Hit
GTACTCTGCGTTGATACCACTGCTT	7458	0.2757178627688092	No Hit
ACATAAGCAGTGGTATCAACGCAGA	7319	0.2705791147231047	No Hit
GTACATGGGATAAGCAGTGGTATCA	6952	0.25701134110602863	No Hit
GTACTGGTTCACTATCGGTCAGTCA	6919	0.25579135056280383	No Hit
CCACTGCTTATGTACTCTGCGTTGA	6341	0.2344230313511691	No Hit
GAGTACATGGGAAGCAGTGGTATCA	6331	0.23405333724716157	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6253	0.23116972323590293	No Hit
GATACCACTGCTTATGTACTCTGCG	6221	0.22998670210307884	No Hit
GTTGATACCACTGCTTATGTACTCT	6182	0.22854489509744952	No Hit
GCTTATCCCATGTACTCTGCGTTGA	6006	0.22203827886691715	No Hit
ATACCACTGCTTATGTACTCTGCGT	5658	0.2091729240474554	No Hit
ATACAGGGTGACAGCCCCGTACACA	5310	0.1963075692279937	No Hit
GGATACCACGTGTCCCGCCCTACTC	5261	0.19449606811835682	No Hit
GCGTTGATACCACTGCTTCCCATGT	4785	0.17689862876759882	No Hit
GTATCAACGCAGAGTACATGGGAAG	4647	0.17179685013229504	No Hit
GAGTACATGATAAGCAGTGGTATCA	4634	0.17131624779708526	No Hit
CAGTGGTATCAACGCAGAGTACATA	4616	0.1706507984098717	No Hit
GGTATCAACGCAGAGTACATGGGAA	4406	0.16288722222571378	No Hit
TACCACTGCTTATGTACTCTGCGTT	4333	0.1601884552664589	No Hit
GGATAAGCAGTGGTATCAACGCAGA	4266	0.15771150476960846	No Hit
TGATACCACTGCTTATGTACTCTGC	4183	0.15464304370634607	No Hit
GTTAATGATAGTGTGTCGAAACACA	4040	0.1493564180190385	No Hit
ATCATTAACTGAATCCATAGGTTAA	4026	0.14883884627342797	No Hit
TATCAACGCAGAGTACATGGGAAGC	4022	0.14869096863182496	No Hit
GCTTATCATGTACTCTGCGTTGATA	3901	0.14421766997333396	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3862	0.14277586296770464	No Hit
CCCCATGTACTCTGCGTTGATACCA	3800	0.14048375952285802	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3776	0.13959649367323995	No Hit
GCAGAGTACATAAGCAGTGGTATCA	3756	0.1388571054652249	No Hit
GATAAGCAGTGGTATCAACGCAGAG	3728	0.13782196197400384	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	3688	0.13634318555797378	No Hit
GGGATAAGCAGTGGTATCAACGCAG	3688	0.13634318555797378	No Hit
GAGTACATGGGATAAGCAGTGGTAT	3490	0.12902324229862486	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3473	0.12839476232181207	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3462	0.12798809880740378	No Hit
GCGTTGATACCACTGCTTATCATGT	3436	0.12702689413698423	No Hit
CAGTGGTATCAACGCAGAGTACATG	3404	0.12584387300416017	No Hit
GTACACAAGCAGTGGTATCAACGCA	3061	0.11316336523670219	No Hit
ATCAACGCAGAGTACATAAGCAGTG	3001	0.11094520061265707	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	2994	0.1106864147398518	No Hit
CCCATATTCAGACAGGATACCACGT	2893	0.10695250428937585	No Hit
ACGCAGAGTACATGATAAGCAGTGG	2772	0.10247920563088483	No Hit
GGTATCAACGCAGAGTACATGGGAT	2741	0.10133315390846152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6969410400752104E-5	0.0	0.0	0.0	0.0
2	3.6969410400752104E-5	0.0	0.0	0.0	0.0
3	3.6969410400752104E-5	0.0	0.0	0.0	0.0
4	3.6969410400752104E-5	0.0	0.0	0.0	0.0
5	2.2181646240451263E-4	0.0	0.0	0.0	0.0
6	2.9575528320601683E-4	0.0	0.0	0.0	0.0
7	2.9575528320601683E-4	0.0	0.0	0.0	0.0
8	3.327246936067689E-4	0.0	0.0	7.393882080150421E-5	0.0
9	3.327246936067689E-4	0.0	0.0	7.393882080150421E-5	0.0
10	4.0666351440827317E-4	0.0	0.0	7.393882080150421E-5	0.0
11	4.0666351440827317E-4	0.0	0.0	1.1090823120225632E-4	0.0
12	4.0666351440827317E-4	0.0	0.0	1.4787764160300841E-4	0.0
13	4.0666351440827317E-4	0.0	0.0	1.8484705200376054E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTATC	45	3.545212E-5	16.886242	13
AGTCGTA	35	0.0021730321	16.283161	11
CTTCGTT	280	0.0	14.926231	12
CGCGTGT	45	6.724386E-4	14.78941	19
CCTACAC	90	2.2828317E-9	14.781201	3
TCCGCGG	40	0.005285933	14.247767	10
CGTATCA	60	2.5734478E-5	14.247767	14
GTCGGAT	40	0.005285933	14.247767	11
CCCGATA	40	0.005285933	14.247767	13
AGGTCAC	100	6.6211214E-10	14.247503	7
GTTGTAT	400	0.0	14.0103035	12
CGTCCTT	90	3.650166E-8	13.720072	9
GTACAAA	685	0.0	13.600177	1
GTCCTAG	170	0.0	13.420583	1
ATGTACG	85	2.693905E-7	13.414873	4
TAAGACT	85	2.693905E-7	13.414873	4
TCACGCG	85	2.7066744E-7	13.409664	10
TTGGACG	50	0.0015018206	13.297915	11
CACGTAG	50	0.0015018206	13.297915	12
GTATAAT	165	0.0	13.251131	1
>>END_MODULE