FastQCFastQC Report
Wed 25 May 2016
SRR1295324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704939
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1221954.517477103919903No Hit
GCTTATGTACTCTGCGTTGATACCA788372.9145574077640934No Hit
CTTATGTACTCTGCGTTGATACCAC526751.9473636928596172No Hit
GTATCAACGCAGAGTACTTTTTTTT400151.4793309571860955No Hit
CCCATGTACTCTGCGTTGATACCAC337981.2494921327246196No Hit
GAGTACATAAGCAGTGGTATCAACG331861.2268668535593594No Hit
TATGTACTCTGCGTTGATACCACTG275151.017213327176694No Hit
GGTATCAACGCAGAGTACTTTTTTT274651.0153648566566567No Hit
GCGTTGATACCACTGCTTATGTACT268810.9937747209826174No Hit
TATCAACGCAGAGTACTTTTTTTTT258800.9567683411714646No Hit
CATAAGCAGTGGTATCAACGCAGAG249080.9208340742619334No Hit
GTACTTTTTTTTTTTTTTTTTTTTT204560.7562462591577851No Hit
ACTCTGCGTTGATACCACTGCTTAT201470.7448227113439527No Hit
ACGCAGAGTACATAAGCAGTGGTAT199580.7378354927782106No Hit
CATGTACTCTGCGTTGATACCACTG199540.7376876151366075No Hit
CTGCTTATGTACTCTGCGTTGATAC197130.7287779872300263No Hit
GTATCAACGCAGAGTACATAAGCAG167200.6181285419005752No Hit
ACGCAGAGTACTTTTTTTTTTTTTT147590.5456315281047003No Hit
TATCAACGCAGAGTACATAAGCAGT144680.5348734296780814No Hit
ATCATGTACTCTGCGTTGATACCAC137520.508403331831143No Hit
GTACATGGGAAGCAGTGGTATCAAC135550.5011203579821948No Hit
GGTATCAACGCAGAGTACATAAGCA130550.4826356527818187No Hit
GTACATGATAAGCAGTGGTATCAAC124050.45860553602132986No Hit
AAGCAGTGGTATCAACGCAGAGTAC118200.43697843093688993No Hit
GTGGTATCAACGCAGAGTACATGGG98500.3641486924474083No Hit
GTGGTATCAACGCAGAGTACATAAG95690.35376028812479693No Hit
ACATAAGCAGTGGTATCAACGCAGA93700.34640337545504724No Hit
GCAGTGGTATCAACGCAGAGTACAT88250.3262550467866373No Hit
GAGTACTTTTTTTTTTTTTTTTTTT82390.3045909722917966No Hit
GTACTCTGCGTTGATACCACTGCTT74790.276494220387225No Hit
CCACTGCTTATGTACTCTGCGTTGA73250.2708009311855092No Hit
GCTTATCCCATGTACTCTGCGTTGA70250.25971010806528355No Hit
GATACCACTGCTTATGTACTCTGCG70100.2591555669092723No Hit
GTACTGGTTCACTATCGGTCAGTCA69930.25852708693245946No Hit
GTACATGGGATAAGCAGTGGTATCA65630.24263024046013607No Hit
ATACCACTGCTTATGTACTCTGCGT64130.23708482890002328No Hit
GAGTACATGGGAAGCAGTGGTATCA63560.2349775725071804No Hit
GTTGATACCACTGCTTATGTACTCT61350.22680733280861418No Hit
ATACAGGGTGACAGCCCCGTACACA59910.22148373771090588No Hit
GCAGAGTACTTTTTTTTTTTTTTTT58670.2168995308212126No Hit
GGATACCACGTGTCCCGCCCTACTC57530.21268501803552686No Hit
CAGTGGTATCAACGCAGAGTACATA55810.20632627944659748No Hit
GCGTTGATACCACTGCTTCCCATGT52990.1959009057135854No Hit
GAGTACATGATAAGCAGTGGTATCA51060.18876580950624025No Hit
TACCACTGCTTATGTACTCTGCGTT49590.1833313061773297No Hit
TGATACCACTGCTTATGTACTCTGC49480.1829246426629214No Hit
GGATAAGCAGTGGTATCAACGCAGA49420.18270282620051692No Hit
CCCCATGTACTCTGCGTTGATACCA48650.179856181599659No Hit
GTATCAACGCAGAGTACATGGGAAG48100.17782286402761763No Hit
GCAGAGTACATAAGCAGTGGTATCA47630.17608530173878228No Hit
GGTATCAACGCAGAGTACATGGGAA45320.16754536793620853No Hit
GCTTATCATGTACTCTGCGTTGATA45310.1675083985258078No Hit
ACGCAGAGTACATGGGAAGCAGTGG44920.16606659152017844No Hit
ACCCTGTATCGCGCGCCTTTCCAGA43830.16203692578649648No Hit
TATCAACGCAGAGTACATGGGAAGC43770.16181510932409196No Hit
GATAAGCAGTGGTATCAACGCAGAG43690.16151935404088597No Hit
ATCATTAACTGAATCCATAGGTTAA43400.16044724113926412No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA42790.15819210710481826No Hit
ACTCTGCGTTGATACCACTGCTTCC42720.157933321232013No Hit
GGGATAAGCAGTGGTATCAACGCAG41780.1544581966543423No Hit
GTTAATGATAGTGTGTCGAAACACA40410.14939338742943925No Hit
GCGTTGATACCACTGCTTATCATGT39260.14514190523335277No Hit
CAGTGGTATCAACGCAGAGTACATG38950.14399585351092944No Hit
ATCAACGCAGAGTACTTTTTTTTTT37690.13933770780043467No Hit
CCCATATTCAGACAGGATACCACGT36740.13582561381236322No Hit
GAGTACATGGGATAAGCAGTGGTAT36170.13371835741952035No Hit
GTGGTATCAACGCAGAGTACTTTTT35250.13031717166265117No Hit
ACGCAGAGTACATGATAAGCAGTGG34630.12802506821780454No Hit
GTACACAAGCAGTGGTATCAACGCA33710.12462388246093535No Hit
ATCAACGCAGAGTACATAAGCAGTG33480.12377358602171805No Hit
GCGTTGATACCACTGCTTATCCCAT30300.11201731351427888No Hit
GCCCAGAGCCTGAATCAGTGTGTGT29630.10954036301742849No Hit
GTATTTAGCCTTGGAGGATGGTCCC29440.1088379442198142No Hit
GGTATCAACGCAGAGTACATGGGAT29440.1088379442198142No Hit
AAAAAGTACTCTGCGTTGATACCAC29380.1086161277574097No Hit
ACTTAGATGTTTCAGTTCCCCCGGT29110.10761795367658937No Hit
TATCAACGCAGAGTACATGATAAGC28910.10687856546857434No Hit
GCCTTGGAGGATGGTCCCCCCATAT28570.10562160551494876No Hit
GGTATCAACGCAGAGTACATGGGGG28430.10510403376933825No Hit
TATTCAGACAGGATACCACGTGTCC28200.10425373733012094No Hit
CTATCGGTCAGTCAGGAGTATTTAG28180.10417979850931944No Hit
GTATCAACGCAGAGTACATGGGATA27910.10318162442849912No Hit
GCTTCCCATGTACTCTGCGTTGATA27520.10173981742286979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG859.931682E-1015.6767361
ACAGATA755.8797013E-815.1939228
GATCGGT851.7274942E-814.52360311
AGCCTAG1250.014.4673861
TAGCCTA400.00525305414.2611735
GACCGTG602.5793563E-514.2443027
CTTATAC1450.013.7847161
GTATTAG707.126444E-613.5971691
TATACGA1051.3569661E-913.582072
TAGGCCT707.208704E-613.5820694
GTCGTTA707.2973835E-613.5660028
GGTGACC707.2973835E-613.5660028
TACGAAT1205.0931703E-1113.4688864
TAGTTAC500.001478780113.3252251
GCTACAC500.001491213713.310433
AGAGCCG500.00149850813.30181319
GCGAATT500.001504575113.29468213
GACTATG500.001504575113.29468211
GTGACCA655.475906E-513.1485879
CTAGCCA1700.012.863024
Produced by FastQC (version 0.11.5)