FastQCFastQC Report
Wed 25 May 2016
SRR1295323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7923715
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA941041.1876247442014258No Hit
GCTTATGTACTCTGCGTTGATACCA578820.7304906852404459No Hit
GTATCAACGCAGAGTACTTTTTTTT365530.4613113924465986No Hit
CTTATGTACTCTGCGTTGATACCAC307830.38849201416254875No Hit
GGTATCAACGCAGAGTACTTTTTTT230530.2909367638790643No Hit
GAGTACATAAGCAGTGGTATCAACG228080.287844779879135No Hit
GTGGTATCAACGCAGAGTACATGGG226280.2855731181649012No Hit
TATGTACTCTGCGTTGATACCACTG207610.26201093805115405No Hit
TATCAACGCAGAGTACTTTTTTTTT194960.24604620433723323No Hit
CCCATGTACTCTGCGTTGATACCAC183130.2311163387375745No Hit
GTACATGATAAGCAGTGGTATCAAC177380.2238596415948832No Hit
GCGTTGATACCACTGCTTATGTACT173540.21901342993785114No Hit
ATCATGTACTCTGCGTTGATACCAC160780.20290987245250494No Hit
CATGTACTCTGCGTTGATACCACTG136250.1719521714246411No Hit
CATAAGCAGTGGTATCAACGCAGAG130510.16470809462480668No Hit
GTATCAACGCAGAGTACATAAGCAG127660.1611112969106032No Hit
ACTCTGCGTTGATACCACTGCTTAT126630.15981140159634719No Hit
ACGCAGAGTACATAAGCAGTGGTAT106580.1345076141683541No Hit
CTGCTTATGTACTCTGCGTTGATAC106510.13441927176835614No Hit
TATCAACGCAGAGTACATAAGCAGT92040.11615763565448783No Hit
GTACATGGGAAGCAGTGGTATCAAC91400.11534993371164914No Hit
ACGCAGAGTACTTTTTTTTTTTTTT88710.1119550614831553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAT5800.010.4812548
TAAGACG1409.677933E-810.1807744
TAGACCG1252.5668578E-69.8821385
ACCGACC1554.0814484E-89.8050448
ACATAAG212900.09.765323
GTACATA225450.09.5267981
TATCCCG1106.828018E-59.5020565
CCGGTCG1106.856376E-59.4984579
CGCACGG1201.7061644E-59.49833712
TACATAA224750.09.448852
GCTTATG131200.09.3991181
CATAAGC218200.09.3757684
GTCCCGC2750.09.3256412
TATACTG25200.09.2758175
CGCCCTA3000.09.18073916
CGCGGCC1451.7130878E-69.17080811
GCGCACG1451.7130878E-69.17080811
TACCGCG1401.0885466E-58.8201628
GCGGCCG1401.08898075E-58.81988312
GTGTAAG9400.08.6986441
Produced by FastQC (version 0.11.5)