FastQCFastQC Report
Wed 25 May 2016
SRR1295323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7923715
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA953861.2038040237439132No Hit
GTATCAACGCAGAGTACTTTTTTTT888171.1209009915172365No Hit
GCTTATGTACTCTGCGTTGATACCA662580.8361986770094584No Hit
GGTATCAACGCAGAGTACTTTTTTT587750.741760651411617No Hit
TATCAACGCAGAGTACTTTTTTTTT524230.6615962335848778No Hit
CTTATGTACTCTGCGTTGATACCAC358900.4529441051325041No Hit
ACGCAGAGTACTTTTTTTTTTTTTT278980.35208232502052383No Hit
GTGGTATCAACGCAGAGTACATGGG246320.31086428525003734No Hit
GAGTACATAAGCAGTGGTATCAACG241960.3053618157644489No Hit
CCCATGTACTCTGCGTTGATACCAC220110.27778636662222206No Hit
TATGTACTCTGCGTTGATACCACTG205540.25939852707978517No Hit
GCGTTGATACCACTGCTTATGTACT200150.25259616227994064No Hit
ATCATGTACTCTGCGTTGATACCAC185580.2342083227375038No Hit
GTACTTTTTTTTTTTTTTTTTTTTT185380.23395591588036674No Hit
GTACATGATAAGCAGTGGTATCAAC171520.21646412068076654No Hit
CATAAGCAGTGGTATCAACGCAGAG161200.20343992685249282No Hit
CATGTACTCTGCGTTGATACCACTG158990.20065083108112797No Hit
ACTCTGCGTTGATACCACTGCTTAT152550.19252333028131374No Hit
GAGTACTTTTTTTTTTTTTTTTTTT138360.17461506376743738No Hit
GTATCAACGCAGAGTACATAAGCAG136360.1720909951960665No Hit
CTGCTTATGTACTCTGCGTTGATAC126200.15926872685350243No Hit
ACGCAGAGTACATAAGCAGTGGTAT124970.15771642468210934No Hit
TATCAACGCAGAGTACATAAGCAGT102580.12945947702561234No Hit
GCAGAGTACTTTTTTTTTTTTTTTT91230.11513538788308261No Hit
GTACATGGGAAGCAGTGGTATCAAC88610.11182885805458677No Hit
GTGGTATCAACGCAGAGTACTTTTT82690.10435761508332897No Hit
GGTATCAACGCAGAGTACATAAGCA82350.10392852342619592No Hit
AAGCAGTGGTATCAACGCAGAGTAC80200.10121514971197224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCGT400.005295066614.24454310
CGCAGCG1909.094947E-1210.4959788
GCGACTA1451.4555553E-810.478744516
AACTGCG1553.6943675E-910.4153657
CGAAGCG750.002658051410.12945314
ACCGACC1554.096546E-89.8026968
CTAGGAC5600.09.6765643
GCTTATG141750.09.6166841
CTGCTCG4150.09.6107779
TCTATAC11100.09.5924473
AGAACCG2106.548362E-119.50668055
CGCGGAG1106.872934E-59.4963628
ACATAAG207300.09.3967363
GGACAGT5600.09.3271966
GTACATA221050.09.2803031
CGGCCGT1856.7575456E-99.23970313
TCTAGAC4250.09.1712663
TACATAA220350.09.1638052
CTAATAC13750.09.1265293
GTCTAGG4950.09.0385361
Produced by FastQC (version 0.11.5)