FastQCFastQC Report
Wed 25 May 2016
SRR1295322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4131262
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG50980.12340054927525777No Hit
GTATCAACGCAGAGTACTTTTTTTT49360.11947922934928841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG802.8737573E-511.87319
GGTATCA31550.010.3956741
TTAGACG800.00450920039.5022774
GTGGTAT13900.09.3699811
GTCCTAG4200.09.0540661
GTACCGT1054.5031987E-49.0460626
CTATACG950.00182081519.0021574
GTCCTAC2651.8189894E-128.9686491
TGGTATC14950.08.6436892
ACCGGGC1000.00291072378.5486338
CGTGCGC1000.00291072378.54863310
TCGCCCA3250.08.47536212
GCGTCAG1803.9951374E-78.4504611
CTAGGAC3300.08.3503853
CGTCAGA2053.8548023E-88.3429582
TCTATAC5500.08.2927963
GTATTAG6550.08.2730661
GTATAGA6450.08.2539391
CTAGACA4750.08.2019654
TCTGTCG2803.45608E-118.1415558
Produced by FastQC (version 0.11.5)