##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295322_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4131262
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.739376248710442	31.0	30.0	33.0	25.0	34.0
2	29.72519874072378	31.0	30.0	33.0	25.0	34.0
3	30.111224366791554	31.0	30.0	34.0	26.0	34.0
4	33.49662306578474	35.0	33.0	37.0	27.0	37.0
5	33.68939636362932	35.0	35.0	37.0	28.0	37.0
6	33.853692406823875	35.0	35.0	37.0	28.0	37.0
7	33.76055960624139	35.0	35.0	37.0	28.0	37.0
8	33.76709344505384	35.0	35.0	37.0	28.0	37.0
9	34.76181830152626	38.0	34.0	39.0	27.0	39.0
10	34.65997847631063	38.0	34.0	39.0	27.0	39.0
11	35.056742709612706	38.0	35.0	39.0	27.0	39.0
12	35.052751193218924	38.0	35.0	39.0	27.0	39.0
13	34.95420672908182	38.0	35.0	39.0	27.0	39.0
14	35.07418677392042	38.0	33.0	40.0	24.0	41.0
15	35.696112471201296	39.0	34.0	41.0	27.0	41.0
16	35.79265899863044	39.0	34.0	41.0	27.0	41.0
17	35.59871584034128	39.0	34.0	40.0	26.0	41.0
18	35.57751311826749	39.0	34.0	40.0	26.0	41.0
19	35.634198218365235	39.0	34.0	41.0	26.0	41.0
20	35.59802476821852	39.0	34.0	41.0	26.0	41.0
21	35.58884694313747	39.0	34.0	40.0	26.0	41.0
22	35.32830355470072	39.0	34.0	40.0	25.0	41.0
23	34.999388564559695	38.0	33.0	40.0	24.0	41.0
24	35.32050763180839	39.0	34.0	40.0	25.0	41.0
25	33.879539472442076	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26861397474973003
1101	2	0.42075311570719975
1101	3	0.3308224895150005
1101	4	0.3875435844347166
1101	5	0.2333572637547121
1101	6	0.20345149759551617
1101	7	0.08357128964679106
1101	8	0.17937263757271893
1101	9	0.05577445848233964
1101	10	0.41900289925449385
1101	11	0.3478026092919393
1101	12	0.4309057835183907
1101	13	0.44383430875997476
1101	14	0.9655986138905917
1101	15	0.6990033474356423
1101	16	0.3593234879652556
1101	17	0.624113015303358
1101	18	0.7525234692066434
1101	19	0.711472029387032
1101	20	0.8494295319697045
1101	21	0.783173911743809
1101	22	0.6096618169122507
1101	23	-1.4621179513287075
1101	24	0.10022278738344426
1101	25	0.41998216288585155
1102	1	0.20321643736807715
1102	2	0.25632733963557186
1102	3	0.26597481344327534
1102	4	0.33733486346973507
1102	5	0.1619299415655746
1102	6	0.1258456086318347
1102	7	0.06335007138849136
1102	8	0.09764918195109829
1102	9	0.3689857043806697
1102	10	0.8664117562151148
1102	11	0.6114972863561974
1102	12	0.40893322955369626
1102	13	0.45907789575259983
1102	14	0.9120822743286965
1102	15	0.712638477420235
1102	16	0.37273946216135556
1102	17	0.7537207982119938
1102	18	0.832195453224216
1102	19	0.7671859770718257
1102	20	0.8668541685390068
1102	21	0.8286957802924633
1102	22	0.635906941204226
1102	23	0.6954365914447607
1102	24	0.7063763693693161
1102	25	0.6154878158167634
1103	1	-0.36700678662460007
1103	2	-1.7287822817503198
1103	3	-0.40973320585850104
1103	4	0.013997890892184728
1103	5	-0.20122347631255622
1103	6	-0.06231526127513831
1103	7	-0.05218312127503566
1103	8	0.1441802880465488
1103	9	0.7856281009123123
1103	10	0.8650983361320002
1103	11	0.46629075286647037
1103	12	0.32029935998053816
1103	13	0.20490902154999446
1103	14	-1.0741425993185771
1103	15	-0.17667000492815532
1103	16	-0.06998442281606998
1103	17	0.41642556312090306
1103	18	0.7151648110941196
1103	19	0.5689541750793765
1103	20	0.598704599174404
1103	21	0.7402382328674477
1103	22	0.2564974328237568
1103	23	1.1102552825622496
1103	24	0.9522836998177908
1103	25	0.7263910272328644
1104	1	-0.06631989276831263
1104	2	-0.022997918283479635
1104	3	-0.46675173596788255
1104	4	-0.25416262552346325
1104	5	-0.20686523656313938
1104	6	-0.17481367163507144
1104	7	-0.17567829509341237
1104	8	-0.15205855080149888
1104	9	-2.1681988817902322
1104	10	-0.7689649870839119
1104	11	-0.5534729383732753
1104	12	-0.13243235212015492
1104	13	-0.016057138790401382
1104	14	-1.834547603883884
1104	15	-0.7522656654846571
1104	16	-0.3828698030333655
1104	17	0.11051851092985032
1104	18	0.15607698373874257
1104	19	0.249987836180658
1104	20	0.45043337342445255
1104	21	0.446163580141949
1104	22	0.5521604554858968
1104	23	0.9596527964101753
1104	24	0.5915160588616359
1104	25	0.4464408192843976
1105	1	-0.2420102271634903
1105	2	-0.16405322150580304
1105	3	-1.4773063433706213
1105	4	-0.6382788239618051
1105	5	-0.5188982670132205
1105	6	-0.24766497910591312
1105	7	-0.22677560186105694
1105	8	-0.20296191359157234
1105	9	0.1877683531987202
1105	10	0.27038573775675445
1105	11	-0.30205600852480075
1105	12	-0.2613031096521894
1105	13	-0.4029699992743616
1105	14	0.425382718901794
1105	15	0.16766545872681604
1105	16	-0.42927525668798694
1105	17	0.18180073206494285
1105	18	0.07578404398600469
1105	19	0.05187830703108176
1105	20	-0.6614665543567639
1105	21	-0.2040220435884308
1105	22	-0.010249646864046724
1105	23	0.06908927114991315
1105	24	0.07565229826636255
1105	25	0.4254081550103379
1106	1	-1.8345338505700859
1106	2	-1.2538628170766621
1106	3	-1.2240563140990588
1106	4	-1.6030089665947003
1106	5	-1.112099631278582
1106	6	-0.9072617394157234
1106	7	-0.747683920424997
1106	8	-0.9442362505605644
1106	9	-0.4484777031282192
1106	10	-1.9739970488163365
1106	11	-1.4725933327360323
1106	12	-1.1035109037272548
1106	13	-1.2383837992377806
1106	14	-0.5826868210921603
1106	15	-1.1800675368776155
1106	16	-1.5756595848724615
1106	17	-0.885889670984497
1106	18	-0.8287638257719152
1106	19	-0.706786343928016
1106	20	-0.3770039341919542
1106	21	-0.46129245034012456
1106	22	-0.31315998804861067
1106	23	-0.8352420863566294
1106	24	-0.9993895293503243
1106	25	-1.8915342655983771
1107	1	-0.12740704617126752
1107	2	0.06505804556176287
1107	3	-0.1226057549318007
1107	4	0.09080804124757691
1107	5	-0.05924469015636191
1107	6	-0.0410662344552577
1107	7	-0.08169735513926213
1107	8	-0.10705740827764032
1107	9	0.4731956827773729
1107	10	0.023618666728161486
1107	11	0.037011452702579106
1107	12	-0.13039831996714923
1107	13	0.0780336173018199
1107	14	-1.2089205529749805
1107	15	-0.5806448426616413
1107	16	-0.27067028465267384
1107	17	-0.11018949186290428
1107	18	0.26538209145159186
1107	19	0.20608607183686445
1107	20	0.30683039415217195
1107	21	0.39574114037516495
1107	22	-0.2970665245850057
1107	23	0.5414411790897091
1107	24	0.31964291242090326
1107	25	-0.012662657177152425
1108	1	-0.2317757033441623
1108	2	0.1022560032186206
1108	3	-0.16741298090442314
1108	4	-0.38845237988273595
1108	5	-0.3370090545864741
1108	6	-0.3461864069327447
1108	7	-0.15498375960583388
1108	8	-0.2820315544369336
1108	9	0.22114240749865388
1108	10	0.18734464522168537
1108	11	-0.09370632271888724
1108	12	-0.09068432266784043
1108	13	-0.25425373802148954
1108	14	-0.0034573310355270337
1108	15	-0.12642321564124614
1108	16	-0.20526913328908591
1108	17	0.17495481906868804
1108	18	0.14958316494140433
1108	19	0.15635297707746076
1108	20	0.3663256150047687
1108	21	0.2651851763573774
1108	22	0.17614410014212467
1108	23	-0.026329081590084513
1108	24	0.08945265610467601
1108	25	-0.8079407961222174
1109	1	-0.9638546495943423
1109	2	-0.782980987143258
1109	3	-0.8770046370634539
1109	4	-0.7372448950232382
1109	5	-1.041169433722601
1109	6	-1.2082242397557579
1109	7	-1.0455462710488135
1109	8	-1.4065961010677697
1109	9	-1.0801579160549082
1109	10	-1.2618104971472732
1109	11	-1.3814968552752873
1109	12	-1.3195071305069348
1109	13	-1.3282820865276435
1109	14	-0.7620197589229676
1109	15	-1.4223568364876655
1109	16	-1.4297489130153167
1109	17	-1.5558461375659434
1109	18	-2.116648392927317
1109	19	-2.3406807058680883
1109	20	-2.007330587045878
1109	21	-1.6871494641527818
1109	22	-1.90126755620863
1109	23	-1.4261070012660255
1109	24	-2.286514557693394
1109	25	-1.3591327764691101
1110	1	0.10016940836216293
1110	2	0.21052334076201618
1110	3	-0.008134827033586589
1110	4	0.27301542293655245
1110	5	0.1585661003940757
1110	6	0.189786282620382
1110	7	0.05170828034371766
1110	8	0.025443699927251373
1110	9	0.33488498157957736
1110	10	0.21291837406923975
1110	11	0.1525772638273324
1110	12	0.2591791696644634
1110	13	0.31390329704801445
1110	14	-2.325265890507495
1110	15	-0.6247941706601168
1110	16	-0.21200333329532128
1110	17	-0.2279533780763714
1110	18	-2.803316609105643
1110	19	-0.5273155717876534
1110	20	-2.141277813062416
1110	21	-0.4008935372706617
1110	22	-1.937287933478963
1110	23	-0.6998947706128718
1110	24	0.0254531552512276
1110	25	0.008760602825120145
1111	1	-0.0699739520121554
1111	2	-1.1571437827886442
1111	3	-0.23202288003808036
1111	4	0.024349643249884423
1111	5	0.11600900849788331
1111	6	0.10174926162306974
1111	7	0.01611704715875817
1111	8	0.10616688626895154
1111	9	-1.6531398955305292
1111	10	-0.24027484321258186
1111	11	-0.10426572731923756
1111	12	-0.044096286735850754
1111	13	0.07405723867698555
1111	14	0.9670338581318916
1111	15	0.427198141187759
1111	16	0.43632746262040456
1111	17	0.04691257037460872
1111	18	0.3204732212817518
1111	19	0.04200593316142687
1111	20	0.48742310353318885
1111	21	-0.39090751167027804
1111	22	-0.10325049280319121
1111	23	0.03190314974644082
1111	24	-0.4889529774118131
1111	25	-1.8259480108519313
1112	1	0.30760779418761786
1112	2	0.5742643865395394
1112	3	0.1890409238526587
1112	4	0.49021979172923835
1112	5	0.2936888614212023
1112	6	0.27027051718039985
1112	7	0.21295496180149343
1112	8	0.2032642438455028
1112	9	-0.2560696681626169
1112	10	0.5987876040339231
1112	11	0.30683790485953466
1112	12	0.22098770563378878
1112	13	0.24596015900333157
1112	14	0.7743079786290039
1112	15	0.40304088249219205
1112	16	0.4131838068945868
1112	17	-3.664270191207674
1112	18	-0.8853695989647576
1112	19	-1.042857809903765
1112	20	0.07994670398259274
1112	21	-0.30463340361658453
1112	22	-1.5360186870467416
1112	23	-1.3500961196155856
1112	24	-0.17334515971932518
1112	25	0.2231531621694458
1113	1	-0.29266398352756084
1113	2	-0.10262820751230706
1113	3	-0.06975909453652562
1113	4	-0.5249310695356968
1113	5	-0.15210270032768847
1113	6	-0.21910622086046772
1113	7	-0.43165138167747585
1113	8	-0.22568938790299597
1113	9	0.2700994931542269
1113	10	0.368345293526005
1113	11	-0.08336009384811405
1113	12	-0.09767986558647834
1113	13	-0.4645129044206726
1113	14	-1.5025558574122968
1113	15	-1.7599391519437617
1113	16	-0.4007008223105757
1113	17	-0.3175782701808032
1113	18	-0.44318357766303507
1113	19	-0.0703679645752402
1113	20	0.2817930575192875
1113	21	-1.5615976019516182
1113	22	-2.1552283968118147
1113	23	-0.7426747243750143
1113	24	-2.426718853059441
1113	25	-0.6214045695692292
1114	1	0.3646926364909824
1114	2	0.4477347030233254
1114	3	0.13574981798280206
1114	4	0.4568002172501977
1114	5	0.27662218915412495
1114	6	0.35625768762511
1114	7	0.396187633359915
1114	8	0.28063049560764597
1114	9	0.6647148349374632
1114	10	0.5370848992734878
1114	11	0.4913042326577184
1114	12	0.4941555563226032
1114	13	0.465806162423668
1114	14	1.2058766034966695
1114	15	0.6720848202122909
1114	16	0.5749100438260726
1114	17	-0.31670363379689803
1114	18	0.11483850334609258
1114	19	-0.29222418706645925
1114	20	-1.3858524899840248
1114	21	-0.4176947455773359
1114	22	-1.2244931149208327
1114	23	-2.8249889410555014
1114	24	-1.9635850308959064
1114	25	-1.2162304088705582
1115	1	0.4693895850403784
1115	2	0.544846429167805
1115	3	0.4291740673186908
1115	4	0.6114079178764698
1115	5	0.5348891113660201
1115	6	0.39607485704183176
1115	7	0.48730164942567455
1115	8	0.5142858703978561
1115	9	0.7811751476966791
1115	10	0.9627373509530841
1115	11	0.6549683563543169
1115	12	0.5215495056197952
1115	13	0.4502544423160515
1115	14	1.1795459129912231
1115	15	0.7590371483178373
1115	16	0.7333594335019029
1115	17	0.6806569425642621
1115	18	0.8867019750165426
1115	19	0.32362449656922365
1115	20	0.8545620107325718
1115	21	-0.21819131126278535
1115	22	0.8608461690305447
1115	23	0.8525066249170408
1115	24	1.0186936109924076
1115	25	1.1146524363509656
1116	1	0.6303807152549545
1116	2	0.7216435591067487
1116	3	0.5404806922646905
1116	4	0.8554553223469199
1116	5	0.7093691009176197
1116	6	0.6111447576403393
1116	7	0.6256272421763001
1116	8	0.581663435064101
1116	9	0.7889553428268243
1116	10	0.7270763650230379
1116	11	0.6854632937124094
1116	12	0.6506923913650482
1116	13	0.19372288799494441
1116	14	1.202388276838633
1116	15	0.852154066875606
1116	16	0.8727399142392898
1116	17	0.9685783394143783
1116	18	0.9205327467602231
1116	19	1.060647573955741
1116	20	0.9595339418490525
1116	21	0.3803616874682092
1116	22	0.48719834702193054
1116	23	-0.5527559105715056
1116	24	-0.11077284528090559
1116	25	-0.6463509297935559
1201	1	0.3448027023464313
1201	2	0.529160187849989
1201	3	0.4783805600092741
1201	4	0.3192900404519534
1201	5	0.3184602085533541
1201	6	0.24094536582612847
1201	7	0.3169131097596747
1201	8	0.26852788744697875
1201	9	0.578734255180251
1201	10	0.8437528859265555
1201	11	0.4587361717154579
1201	12	0.5023673789703267
1201	13	0.40321546759698634
1201	14	1.0298026586807794
1201	15	0.755936202532645
1201	16	0.4489431480801045
1201	17	0.5772085569626029
1201	18	0.5106729185886962
1201	19	0.7504261035474968
1201	20	0.8650060378474791
1201	21	0.7927239664402279
1201	22	0.8409301382610082
1201	23	0.44114205777455595
1201	24	0.7603495425395295
1201	25	0.828511534930449
1202	1	0.3070508770170086
1202	2	0.6062757903506153
1202	3	0.4204303555083797
1202	4	0.42081169287853015
1202	5	0.3391338581295855
1202	6	0.17516217441551873
1202	7	0.15124736276159467
1202	8	0.1703570850916165
1202	9	0.7569650462632111
1202	10	-0.0801501723485103
1202	11	0.2749245457362477
1202	12	0.3027273750910666
1202	13	0.2903827130258918
1202	14	1.001748193604584
1202	15	0.7136863015677477
1202	16	0.5335795087181907
1202	17	-1.3913665844727632
1202	18	-0.03467711517090777
1202	19	0.4467205416797668
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2210	19	0.953450493563686
2210	20	1.035974227776606
2210	21	1.1715392318198
2210	22	1.3450013651842099
2210	23	1.745900322476075
2210	24	1.372644916267305
2210	25	1.3257993287187517
2211	1	0.1720057926480436
2211	2	-0.471186593687964
2211	3	-0.027329089394637407
2211	4	0.26445698683664887
2211	5	0.3247811008859216
2211	6	0.19183228389024976
2211	7	0.24897020320395313
2211	8	0.30051394486791594
2211	9	0.8034030860840176
2211	10	0.8608341874528875
2211	11	0.5121803866195975
2211	12	0.31450750104530556
2211	13	0.27882393054916577
2211	14	-0.913389800615434
2211	15	-0.021728879977523263
2211	16	0.2768025474268896
2211	17	0.36808455687440045
2211	18	0.22128977777362024
2211	19	0.6057795875266194
2211	20	0.671654052475084
2211	21	0.609103869345148
2211	22	-0.108466313597539
2211	23	0.4962579886584777
2211	24	-0.11864153009972966
2211	25	0.3824586792494671
2212	1	0.10301597091960701
2212	2	-0.9100575727443498
2212	3	-0.26336869626880244
2212	4	0.2308683972695178
2212	5	0.2508745389107929
2212	6	0.17241411393420947
2212	7	0.13736698784494195
2212	8	0.1600557266020104
2212	9	-2.0466209772743156
2212	10	0.1060346077103489
2212	11	0.16004803432563364
2212	12	0.11702463590795986
2212	13	0.1283503229365266
2212	14	0.7359096902620479
2212	15	0.42184052202868827
2212	16	0.4308872147066154
2212	17	0.4083068181970475
2212	18	0.2290780506272938
2212	19	-0.43533350261134984
2212	20	0.3377114519199864
2212	21	0.39615248355266175
2212	22	-1.5798122461787045
2212	23	-0.294283880118023
2212	24	0.37357026372872326
2212	25	-0.29956608987254185
2213	1	0.1995952160550658
2213	2	0.4840865883852139
2213	3	0.22689322576804472
2213	4	0.46823366422382406
2213	5	0.48392963683838985
2213	6	0.45970098529607384
2213	7	0.3807007900134778
2213	8	0.3599372125095499
2213	9	0.7877328330025648
2213	10	0.8271092879249125
2213	11	0.20138195131005432
2213	12	0.19427310287291988
2213	13	0.18190412856976224
2213	14	-1.6075456975767608
2213	15	-0.37130062932840247
2213	16	0.11914365807763971
2213	17	-2.0075708117260262
2213	18	-0.903673079826568
2213	19	-0.49044974534128016
2213	20	0.26800798494414124
2213	21	-0.037829821916481876
2213	22	0.4996938194838876
2213	23	1.0351321764417492
2213	24	0.7612092539988566
2213	25	-0.67200450776064
2214	1	0.3456900838900836
2214	2	0.23061111899354003
2214	3	0.23331981502531818
2214	4	0.5444269882345125
2214	5	0.4586186180353451
2214	6	0.40592465518825804
2214	7	0.45649511404810283
2214	8	0.2997881295883005
2214	9	0.8155469848904744
2214	10	0.6809014507423612
2214	11	0.07604970743453521
2214	12	0.08220033919193526
2214	13	0.2036172164185004
2214	14	1.1124786173152614
2214	15	0.6861290157028606
2214	16	0.5789384274508151
2214	17	0.43852556876048965
2214	18	0.46755546477034216
2214	19	-0.8174575362811609
2214	20	0.2303814545887377
2214	21	0.5908149107803453
2214	22	0.8371133171083471
2214	23	1.4128433481490106
2214	24	1.0641756943650122
2214	25	1.1040875427064734
2215	1	0.04284740908701323
2215	2	0.4601620127442665
2215	3	0.2946821037649592
2215	4	0.46584674096012435
2215	5	0.4551390853588728
2215	6	0.3662971059869946
2215	7	0.3665141626320576
2215	8	0.26198373704848876
2215	9	0.48039913267839296
2215	10	0.7946563728172222
2215	11	0.5201055166031878
2215	12	0.32758526281899947
2215	13	0.2219241200851414
2215	14	1.1798110427072217
2215	15	0.6753954990926587
2215	16	0.5492152976119513
2215	17	0.7190285994531962
2215	18	0.5904541617640646
2215	19	-0.25388910588317515
2215	20	-1.2578442084585078
2215	21	-1.7371368241475338
2215	22	-0.0316909734666595
2215	23	0.6926303521178454
2215	24	0.7215883732012145
2215	25	0.15204736987288214
2216	1	0.20448432624396773
2216	2	-0.16599552637264026
2216	3	-0.03599609641740287
2216	4	0.4404083520024713
2216	5	0.45431578421588625
2216	6	0.2209300324275958
2216	7	0.26925500200555774
2216	8	0.2308411590444237
2216	9	0.5240445865661627
2216	10	-1.4109619144791594
2216	11	-0.19146228158114553
2216	12	0.013573892242895624
2216	13	0.30062980622048485
2216	14	-0.22658387983455697
2216	15	-0.1809323901032016
2216	16	0.20711242537809227
2216	17	0.5223969517624738
2216	18	0.47741273795885064
2216	19	-1.196468478643979
2216	20	-0.7015564024009322
2216	21	-0.6029341755319706
2216	22	-0.5133740220793612
2216	23	-4.240504520799057
2216	24	-1.9291881642804327
2216	25	-0.8178055478211164
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	30.0
4	45.0
5	69.0
6	103.0
7	345.0
8	201.0
9	553.0
10	1483.0
11	3620.0
12	7223.0
13	11937.0
14	17023.0
15	21690.0
16	25666.0
17	28241.0
18	29955.0
19	31386.0
20	32202.0
21	33405.0
22	35253.0
23	37231.0
24	40390.0
25	44620.0
26	50232.0
27	58016.0
28	67491.0
29	82062.0
30	99933.0
31	121653.0
32	156867.0
33	204398.0
34	270549.0
35	362327.0
36	496053.0
37	703736.0
38	1055270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.904012310238556	21.072877406826542	13.80025320098664	23.222857081948263
2	15.712447802474491	21.169921829605585	40.319489529505645	22.79814083841428
3	17.942569655956923	26.28004950148633	30.349200491915575	25.428180350641178
4	12.720269290869155	17.643312610889854	38.56331064462676	31.07310745361423
5	13.63535421368362	37.52532225406264	35.345931354018006	13.493392178235734
6	30.642293566653994	38.61414207930739	17.626249543034636	13.117314811003968
7	25.80191320765041	32.884863501584974	23.70082760721209	17.61239568355252
8	23.438859460604778	37.089445167539424	21.57278203226685	17.898913339588944
9	24.459778186749322	17.327247191011235	22.275692561022858	35.93728206121658
10	15.129085625228347	29.940030162874194	33.828711773132966	21.102172438764498
11	31.7486903997513	25.814523415913932	24.049383720378437	18.387402463956334
12	20.907552250723093	27.484452914731982	31.474268233250218	20.133726601294704
13	26.23900218346215	24.394826516692664	26.991132015443554	22.375039284401634
14	21.098240560107094	23.575588885423308	28.060882005939707	27.26528854852989
15	22.53941040580005	31.091868016911967	24.333425351485126	22.03529622580286
16	22.01413821965545	31.15214360096295	25.818145896722765	21.01557228265883
17	20.426991515759592	30.302504983964635	28.355042290459213	20.91546120981656
18	20.487180182527315	29.997443106702836	29.130641751161985	20.384734959607865
19	21.156373320421256	28.913739256748578	29.44401404188355	20.485873380946618
20	22.17968560671812	28.56971659283889	28.771868408125872	20.47872939231712
21	22.580973152144626	28.843778120090903	27.939311321195497	20.635937406568974
22	21.699043499966468	28.452649895130488	29.166241967125956	20.68206463777709
23	21.995668695124802	28.708900722570117	28.522671560468186	20.772759021836894
24	21.639428325308668	28.05325210131438	28.85799431579238	21.449325257584572
25	22.174754731328214	28.266591967967443	28.32516033605691	21.233492964647436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4288.0
1	4288.0
2	4430.0
3	4572.0
4	4572.0
5	4572.0
6	6299.0
7	8026.0
8	8026.0
9	8026.0
10	12788.5
11	17551.0
12	17551.0
13	17551.0
14	29009.5
15	40468.0
16	40468.0
17	40468.0
18	63856.0
19	87244.0
20	87244.0
21	87244.0
22	122876.5
23	158509.0
24	158509.0
25	158509.0
26	204641.5
27	250774.0
28	250774.0
29	250774.0
30	300343.0
31	349912.0
32	349912.0
33	349912.0
34	392081.0
35	434250.0
36	434250.0
37	434250.0
38	462424.5
39	490599.0
40	490599.0
41	490599.0
42	490132.5
43	489666.0
44	489666.0
45	489666.0
46	472250.0
47	454834.0
48	454834.0
49	454834.0
50	432889.0
51	410944.0
52	410944.0
53	410944.0
54	374376.5
55	337809.0
56	337809.0
57	337809.0
58	293524.5
59	249240.0
60	249240.0
61	249240.0
62	207918.5
63	166597.0
64	166597.0
65	166597.0
66	130530.0
67	94463.0
68	94463.0
69	94463.0
70	70997.0
71	47531.0
72	47531.0
73	47531.0
74	34404.0
75	21277.0
76	21277.0
77	21277.0
78	14818.0
79	8359.0
80	8359.0
81	8359.0
82	5745.0
83	3131.0
84	3131.0
85	3131.0
86	2039.5
87	948.0
88	948.0
89	948.0
90	583.5
91	219.0
92	219.0
93	219.0
94	129.0
95	39.0
96	39.0
97	39.0
98	25.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0802660300895949
2	0.019316131487182368
3	0.03110429694364579
4	0.03875329136714156
5	0.04814509464662372
6	0.018202670273635514
7	0.022293429949492433
8	0.03962469579513475
9	0.040229837759018915
10	0.024665586447918338
11	0.024060444484034174
12	0.018033230523747952
13	0.027207182696231805
14	0.04274722832877702
15	0.03974572418791159
16	0.025149700019025667
17	0.024592969412252235
18	0.03025709819420797
19	0.018444727059189175
20	0.01701659202442256
21	0.013095272098453207
22	0.021567259592831442
23	0.020986323307502646
24	0.021083146021724113
25	0.1079815320354894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4131262.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.16764486238857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.20318633500264	70.71041717835575
2	8.647452935460032	13.86491871788359
3	1.6309649416216956	3.9225185466880315
4	0.5523997347380839	1.7713834302624145
5	0.27036205085170567	1.083714443847329
6	0.1721079858934996	0.8278495134654635
7	0.10835595759365879	0.6080649348964732
8	0.07396370184800619	0.47435966259668416
9	0.05457762531197902	0.39378237150989187
>10	0.26631441556928764	3.7182019719299046
>50	0.013936968590691037	0.7752280146237073
>100	0.005407199232558593	0.7326224301005189
>500	6.063428328386246E-4	0.325413416257871
>1k	3.3348833226527546E-4	0.6676209144876974
>5k	3.0317121114987273E-5	0.12390445309463646
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5098	0.12340054927525777	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4936	0.11947922934928841	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.4205678555366375E-5	0.0	0.0	0.0
5	4.841135711073275E-5	2.4205678555366375E-5	0.0	0.0	0.0
6	4.841135711073275E-5	2.4205678555366375E-5	0.0	0.0	0.0
7	4.841135711073275E-5	2.4205678555366375E-5	0.0	2.4205678555366375E-5	0.0
8	4.841135711073275E-5	2.4205678555366375E-5	0.0	2.4205678555366375E-5	0.0
9	4.841135711073275E-5	2.4205678555366375E-5	0.0	7.261703566609913E-5	0.0
10	4.841135711073275E-5	2.4205678555366375E-5	0.0	9.68227142214655E-5	0.0
11	4.841135711073275E-5	2.4205678555366375E-5	0.0	1.2102839277683187E-4	0.0
12	4.841135711073275E-5	2.4205678555366375E-5	0.0	1.2102839277683187E-4	0.0
13	4.841135711073275E-5	2.4205678555366375E-5	0.0	1.2102839277683187E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	80	2.8737573E-5	11.8731	9
GGTATCA	3155	0.0	10.395674	1
TTAGACG	80	0.0045092003	9.502277	4
GTGGTAT	1390	0.0	9.369981	1
GTCCTAG	420	0.0	9.054066	1
GTACCGT	105	4.5031987E-4	9.046062	6
CTATACG	95	0.0018208151	9.002157	4
GTCCTAC	265	1.8189894E-12	8.968649	1
TGGTATC	1495	0.0	8.643689	2
ACCGGGC	100	0.0029107237	8.548633	8
CGTGCGC	100	0.0029107237	8.548633	10
TCGCCCA	325	0.0	8.475362	12
GCGTCAG	180	3.9951374E-7	8.450461	1
CTAGGAC	330	0.0	8.350385	3
CGTCAGA	205	3.8548023E-8	8.342958	2
TCTATAC	550	0.0	8.292796	3
GTATTAG	655	0.0	8.273066	1
GTATAGA	645	0.0	8.253939	1
CTAGACA	475	0.0	8.201965	4
TCTGTCG	280	3.45608E-11	8.141555	8
>>END_MODULE