FastQCFastQC Report
Wed 25 May 2016
SRR1295322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4131262
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT138200.3345224776351633No Hit
GGTATCAACGCAGAGTACTTTTTTT116500.28199615517001825No Hit
TATCAACGCAGAGTACTTTTTTTTT81610.197542542690345No Hit
GTGGTATCAACGCAGAGTACATGGG60980.14760622783062416No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56870.13765769394436858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3400.011.4654093
GGTATCA42900.011.2948961
CGTATAC856.5476436E-410.0671883
GCAGCGT3700.010.0342451
GTGGTAT19100.09.669191
TGGTATC18350.09.4819942
TATACTG9150.09.2481225
TAGACAG5250.09.2362475
CTACGTC850.00738856068.9486124
TAGACTG5350.08.8858895
GTATTAG6250.08.8342511
CTACCCT3350.08.7983574
GCCTAGA4700.08.7094831
GTCTAAG2751.8189894E-128.6542431
ACTTCGC1000.00291747888.5464448
TTAGGAC3350.08.5145393
TCTATAC5050.08.4723863
CAGCGTC4650.08.383312
AGGACCG1150.00110211438.267745
AGCACCG1752.413397E-68.149635
Produced by FastQC (version 0.11.5)