FastQCFastQC Report
Wed 25 May 2016
SRR1295321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812863
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA709573.9140850687558846No Hit
GCTTATGTACTCTGCGTTGATACCA411392.2692834483355884No Hit
CTTATGTACTCTGCGTTGATACCAC266441.4697194437748469No Hit
GAGTACATAAGCAGTGGTATCAACG189331.0443701482130752No Hit
TATGTACTCTGCGTTGATACCACTG173640.9578219644838027No Hit
CCCATGTACTCTGCGTTGATACCAC165320.9119277077197779No Hit
GCGTTGATACCACTGCTTATGTACT144880.7991778749966214No Hit
CATAAGCAGTGGTATCAACGCAGAG123220.6796983555845092No Hit
CATGTACTCTGCGTTGATACCACTG117300.6470428267331839No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA113320.625088602944624No Hit
ACTCTGCGTTGATACCACTGCTTAT109090.6017553449984914No Hit
ACGCAGAGTACATAAGCAGTGGTAT103290.569761752542801No Hit
CTGCTTATGTACTCTGCGTTGATAC103210.5693204616123777No Hit
GTACATGATAAGCAGTGGTATCAAC93650.5165861954267918No Hit
GTATCAACGCAGAGTACATAAGCAG92430.5098565087378363No Hit
GTATCAACGCAGAGTACTTTTTTTT91960.5072639245215993No Hit
ATCATGTACTCTGCGTTGATACCAC88650.4890055122753346No Hit
GTACATGGGAAGCAGTGGTATCAAC80830.4458693238264557No Hit
TATCAACGCAGAGTACATAAGCAGT80070.44167705998743423No Hit
GGTATCAACGCAGAGTACATAAGCA77200.4258457478584978No Hit
AAGCAGTGGTATCAACGCAGAGTAC64210.35419113303101224No Hit
GTGGTATCAACGCAGAGTACATGGG61890.34139369604873615No Hit
GGTATCAACGCAGAGTACTTTTTTT60920.3360430435173535No Hit
GTGGTATCAACGCAGAGTACATAAG57790.31877753586454133No Hit
TATCAACGCAGAGTACTTTTTTTTT56150.3097310717908634No Hit
GTACTGGTTCACTATCGGTCAGTCA50780.2801094180861985No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49810.2747587655548158No Hit
GTACTCTGCGTTGATACCACTGCTT49260.2717248904081555No Hit
GCAGTGGTATCAACGCAGAGTACAT49190.2713387608440351No Hit
ACATAAGCAGTGGTATCAACGCAGA45640.25175647580650057No Hit
GTACATGGGATAAGCAGTGGTATCA40980.22605127910934253No Hit
CCACTGCTTATGTACTCTGCGTTGA40370.22268643576486474No Hit
GATACCACTGCTTATGTACTCTGCG38300.2112680329401615No Hit
GTTGATACCACTGCTTATGTACTCT37330.20591738040877883No Hit
GAGTACATGGGAAGCAGTGGTATCA36800.20299382799472435No Hit
GCTTATCCCATGTACTCTGCGTTGA36730.20260769843060397No Hit
ATACAGGGTGACAGCCCCGTACACA36460.2011183415404253No Hit
GAGTACATGATAAGCAGTGGTATCA35380.19516091397971055No Hit
ATACCACTGCTTATGTACTCTGCGT35340.1949402685144989No Hit
GGATACCACGTGTCCCGCCCTACTC35040.19328542752541145No Hit
CAGTGGTATCAACGCAGAGTACATA29390.16211925556426493No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28740.1585337667545755No Hit
GCTTATCATGTACTCTGCGTTGATA28580.15765118489372887No Hit
GTATCAACGCAGAGTACATGGGAAG28190.15549989160791522No Hit
GATAAGCAGTGGTATCAACGCAGAG27970.1542863415492511No Hit
GCGTTGATACCACTGCTTCCCATGT27260.1503698845417442No Hit
GGTATCAACGCAGAGTACATGGGAA27130.1496527867798063No Hit
GGATAAGCAGTGGTATCAACGCAGA26960.14871504355265677No Hit
TACCACTGCTTATGTACTCTGCGTT26620.14683955709835766No Hit
TGATACCACTGCTTATGTACTCTGC26330.14523987747557318No Hit
GCGTTGATACCACTGCTTATCATGT25540.14088212953764295No Hit
GCAGAGTACATAAGCAGTGGTATCA24690.13619341340189522No Hit
ACCCTGTATCGCGCGCCTTTCCAGA24000.13238727912699416No Hit
CAGTGGTATCAACGCAGAGTACATG23340.1287466289510018No Hit
TATCAACGCAGAGTACATGGGAAGC23140.12764340162494353No Hit
GAACAAAAAAAAAAAAAAAAAAAAA22560.1244440423793745No Hit
ATCATTAACTGAATCCATAGGTTAA22060.1216859740642288No Hit
ACGCAGAGTACATGGGAAGCAGTGG21920.12091371493598799No Hit
ACGCAGAGTACATGATAAGCAGTGG21760.12003113307514136No Hit
GGGATAAGCAGTGGTATCAACGCAG21620.11925887394690056No Hit
GAGTACATGGGATAAGCAGTGGTAT21240.11716274202738983No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA21200.11694209656217817No Hit
CCCATATTCAGACAGGATACCACGT21070.11622499880024027No Hit
GTATTTAGCCTTGGAGGATGGTCCC21040.11605951470133154No Hit
CCCCATGTACTCTGCGTTGATACCA20520.11319112365358No Hit
GGTTAATGAGGCGAACCGGGGGAAC20380.11241886452533921No Hit
TATCAACGCAGAGTACATGATAAGC20230.11159144403079548No Hit
GTTAATGATAGTGTGTCGAAACACA19640.10833692341892355No Hit
TCTAAGTACCCCGAGGAAAAGAAAT19360.10679240516244194No Hit
ACTCTGCGTTGATACCACTGCTTCC19340.10668208242983611No Hit
CATGTAAGCAGTGGTATCAACGCAG19090.10530304827226326No Hit
GTATCAACGCAGAGTACATGATAAG18950.10453078914402247No Hit
ATCAACGCAGAGTACATAAGCAGTG18700.10315175498644961No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18640.10282078678863213No Hit
GTACACAAGCAGTGGTATCAACGCA18530.10221401175930006No Hit
GAGTACATGTAAGCAGTGGTATCAA18430.10166239809627092No Hit
GCTTACATGTACTCTGCGTTGATAC18180.10028336393869808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGACT401.2815906E-519.0065444
CGGAATT350.00217500316.28053515
GACTGTC551.1301472E-515.5430837
AGGCCTC508.689651E-515.2052355
CCCTATA755.824586E-815.2048152
GTACAAA5350.013.862511
GTTGTAT2900.013.42861612
GCCCAAA500.001489924513.31156351
GTATATG500.001489924513.31156351
GTACTCG1001.002627E-813.304584
TGCCCAA500.001501439613.29797110
GCAGTTG3050.013.0799729
GTGCGCG802.0033513E-613.0605089
GGGTGCG1102.75395E-912.9525697
CAGTTGT3200.012.76367810
CACGGCA604.0978755E-412.6647369
TAGTGCG604.0978755E-412.6647369
CTTCGTT1352.7284841E-1112.66438512
CTTAGGA1301.9645086E-1012.4270132
TACAAAA7800.012.4270132
Produced by FastQC (version 0.11.5)