FastQCFastQC Report
Wed 25 May 2016
SRR1295321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812863
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA730514.029592969794187No Hit
GCTTATGTACTCTGCGTTGATACCA477312.6329071750043993No Hit
CTTATGTACTCTGCGTTGATACCAC318511.756944678114121No Hit
GTATCAACGCAGAGTACTTTTTTTT241341.331264414354532No Hit
CCCATGTACTCTGCGTTGATACCAC207331.1436606075583207No Hit
GAGTACATAAGCAGTGGTATCAACG202801.1186725086231006No Hit
GCGTTGATACCACTGCTTATGTACT165880.9150167442327414No Hit
TATGTACTCTGCGTTGATACCACTG165590.9134170646099566No Hit
GGTATCAACGCAGAGTACTTTTTTT164100.9051980210308225No Hit
CATAAGCAGTGGTATCAACGCAGAG154530.8524085934789335No Hit
TATCAACGCAGAGTACTTTTTTTTT150130.8281375923056513No Hit
CATGTACTCTGCGTTGATACCACTG138610.7645916983246941No Hit
ACTCTGCGTTGATACCACTGCTTAT133440.7360732719460874No Hit
ACGCAGAGTACATAAGCAGTGGTAT124170.6849386853832861No Hit
CTGCTTATGTACTCTGCGTTGATAC122110.6735754439248857No Hit
GTACTTTTTTTTTTTTTTTTTTTTT120000.6619363956349708No Hit
GTATCAACGCAGAGTACATAAGCAG102490.5653488432385679No Hit
ATCATGTACTCTGCGTTGATACCAC100740.555695604135558No Hit
GTACATGATAAGCAGTGGTATCAAC91330.5037887584445156No Hit
ACGCAGAGTACTTTTTTTTTTTTTT91060.5022994015543369No Hit
TATCAACGCAGAGTACATAAGCAGT88460.48795744631557925No Hit
GGTATCAACGCAGAGTACATAAGCA82570.4554674015631628No Hit
GTACATGGGAAGCAGTGGTATCAAC77130.4254596182943775No Hit
AAGCAGTGGTATCAACGCAGAGTAC74180.40918701523501777No Hit
GTGGTATCAACGCAGAGTACATGGG65410.360810496987362No Hit
GTGGTATCAACGCAGAGTACATAAG61950.3417246642465537No Hit
ACATAAGCAGTGGTATCAACGCAGA59030.3256175452861027No Hit
GCAGTGGTATCAACGCAGAGTACAT56720.3128752696701295No Hit
GTACTGGTTCACTATCGGTCAGTCA52820.291362336811993No Hit
GTACTCTGCGTTGATACCACTGCTT49240.2716145676755497No Hit
CCACTGCTTATGTACTCTGCGTTGA48370.26681552880719617No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46000.25374228499340545No Hit
GATACCACTGCTTATGTACTCTGCG45630.2517013144401976No Hit
GCTTATCCCATGTACTCTGCGTTGA42080.23211902940266307No Hit
ATACAGGGTGACAGCCCCGTACACA41950.2314019316407252No Hit
GTACATGGGATAAGCAGTGGTATCA40890.22555482681261627No Hit
GGATACCACGTGTCCCGCCCTACTC40320.22241062893335017No Hit
ATACCACTGCTTATGTACTCTGCGT39510.2179425582628141No Hit
GTTGATACCACTGCTTATGTACTCT37770.20834448052610705No Hit
GAGTACATGATAAGCAGTGGTATCA37410.20635867133920213No Hit
GAGTACATGGGAAGCAGTGGTATCA36620.2020009234012719No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35800.19747769136443297No Hit
CAGTGGTATCAACGCAGAGTACATA34930.1926786524960794No Hit
GCTTATCATGTACTCTGCGTTGATA33670.18572832034191222No Hit
GCAGAGTACTTTTTTTTTTTTTTTT33160.18291509066046358No Hit
GCGTTGATACCACTGCTTCCCATGT32670.1802121837116208No Hit
TACCACTGCTTATGTACTCTGCGTT31410.1732618515574536No Hit
ACCCTGTATCGCGCGCCTTTCCAGA30800.16989700821297582No Hit
GTATCAACGCAGAGTACATGGGAAG30450.16796636039237384No Hit
GATAAGCAGTGGTATCAACGCAGAG30250.16686313306631556No Hit
CTTATACACATCTCCGAGCCCACGA30100.16603571257177183No Hit
TGATACCACTGCTTATGTACTCTGC29930.1650979693446223No Hit
GGATAAGCAGTGGTATCAACGCAGA29690.16377409655335234No Hit
GCGTTGATACCACTGCTTATCATGT29540.16294667605880864No Hit
GCAGAGTACATAAGCAGTGGTATCA28040.1546724711133715No Hit
GGTATCAACGCAGAGTACATGGGAA27910.1539553733514336No Hit
ACGCAGAGTACATGGGAAGCAGTGG27140.14970794814610922No Hit
ATCATTAACTGAATCCATAGGTTAA26360.14540536157448192No Hit
CAGTGGTATCAACGCAGAGTACATG26320.14518471610927025No Hit
ACGCAGAGTACATGATAAGCAGTGG26170.14435729561472654No Hit
CCCCATGTACTCTGCGTTGATACCA25880.14275761599194203No Hit
TATCAACGCAGAGTACATGGGAAGC25740.14198535686370123No Hit
CCCATATTCAGACAGGATACCACGT25210.13906180444964678No Hit
ACTCTGCGTTGATACCACTGCTTCC24690.13619341340189522No Hit
ATCAACGCAGAGTACTTTTTTTTTT24550.13542115427365445No Hit
GGGATAAGCAGTGGTATCAACGCAG24340.13426276558129321No Hit
GAGTACATGGGATAAGCAGTGGTAT22710.12527146287391822No Hit
CATGTAAGCAGTGGTATCAACGCAG22540.12433371964676868No Hit
TATCAACGCAGAGTACATGATAAGC22440.12378210598373952No Hit
ATCAACGCAGAGTACATAAGCAGTG21440.11826596935344812No Hit
GTGGTATCAACGCAGAGTACTTTTT21430.1182108079871452No Hit
GCTTACATGTACTCTGCGTTGATAC21400.11804532388823645No Hit
GTATTTAGCCTTGGAGGATGGTCCC21110.11644564426545195No Hit
TCTAAGTACCCCGAGGAAAAGAAAT20670.11401854414812371No Hit
GTTAATGATAGTGTGTCGAAACACA20350.11225338042643046No Hit
GCCTTGGAGGATGGTCCCCCCATAT20320.11208789632752172No Hit
GGTTAATGAGGCGAACCGGGGGAAC20030.1104882167047372No Hit
GAGTACATGTAAGCAGTGGTATCAA19690.10861273025043812No Hit
AAAAAGTACTCTGCGTTGATACCAC19540.10778530975589441No Hit
GGTATCAACGCAGAGTACATGGGGG19530.1077301483895915No Hit
ATGATAAGCAGTGGTATCAACGCAG19460.10734401882547111No Hit
GTACACAAGCAGTGGTATCAACGCA19450.10728885745916819No Hit
GCGTTGATACCACTGCTTATCCCAT19320.10657175969723029No Hit
GTATCAACGCAGAGTACATGATAAG19300.10646143696462446No Hit
TATTCAGACAGGATACCACGTGTCC18910.10431014367881081No Hit
CTATCGGTCAGTCAGGAGTATTTAG18760.1034827231842671No Hit
GCCCAGAGCCTGAATCAGTGTGTGT18680.1030414322538438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3500.017.6783081
AGACCGA350.002175318316.2801826
GTTCGCA551.1321545E-515.5401749
TAGGCCT508.6708416E-515.2090994
GCGGGCA456.762127E-414.77805719
TCGCACA456.780539E-414.772758511
CCCCTAT602.520556E-514.2786331
AGGCCTC400.005258630514.2585315
GCTAGCT400.005258630514.2585313
AACAACC801.2941746E-714.24515910
ACGGGTC400.005291464314.24515912
GGCCCCC400.005291464314.24515913
TGCGCTA400.005291464314.24515912
GCTGTAA400.005291464314.2451598
GCGCTAC400.005291464314.24515913
TATACAC4600.014.0518863
ACTGAGT759.712385E-713.92868
TGACCGG707.2896764E-613.56681818
TACGGCC852.6992348E-713.41201819
CAGCGTA500.001503559913.29548310
Produced by FastQC (version 0.11.5)