FastQCFastQC Report
Wed 25 May 2016
SRR1295320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4268831
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50390.1180416840113839No Hit
GTGGTATCAACGCAGAGTACATGGG46090.10796866870578854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT855.313068E-511.1794844
TGTACTG5800.09.3387245
CTAGGAC4000.09.26499753
GTCTAGA4800.09.1114281
TAACCCG1054.4834398E-49.0500585
GGTATCA35050.09.0328761
CTAGACT4150.08.9301194
GTGTTAC3900.08.7762261
TATTCCG1304.3585926E-58.7715965
TAGGACA5550.08.7320834
TAGACTG5750.08.593625
GTAGGAC3100.08.5829583
TCTAGAC4350.08.5195383
TCTACAC5650.08.4093473
GTCTTAC3850.08.3963021
GTCTCGC2951.8189894E-128.379561
CCGTGCA1252.7504298E-48.358539
TAGACAG5950.08.304765
TATACTG11350.08.2885785
GTATTAA8050.08.2674581
Produced by FastQC (version 0.11.5)