##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295320_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4268831
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.15363714328349	31.0	30.0	33.0	26.0	34.0
2	30.11805667640626	31.0	30.0	34.0	26.0	34.0
3	30.444294234182614	31.0	30.0	34.0	26.0	34.0
4	33.812307866017655	35.0	35.0	37.0	28.0	37.0
5	33.98959012432209	35.0	35.0	37.0	28.0	37.0
6	34.23687445110851	36.0	35.0	37.0	30.0	37.0
7	34.19188367963032	37.0	35.0	37.0	30.0	37.0
8	34.264107433627615	37.0	35.0	37.0	30.0	37.0
9	35.2253307755683	39.0	35.0	39.0	27.0	39.0
10	35.17034288778356	39.0	35.0	39.0	27.0	39.0
11	35.546903590233484	39.0	35.0	39.0	29.0	39.0
12	35.53950273505791	39.0	35.0	39.0	29.0	39.0
13	35.443942849927765	39.0	35.0	39.0	28.0	39.0
14	35.65218370087736	39.0	34.0	41.0	25.0	41.0
15	36.27061670982056	39.0	36.0	41.0	27.0	41.0
16	36.37979648292472	39.0	36.0	41.0	27.0	41.0
17	36.189107509760866	39.0	36.0	41.0	27.0	41.0
18	36.169509872843406	39.0	36.0	41.0	27.0	41.0
19	36.230492376015825	39.0	35.0	41.0	27.0	41.0
20	36.20392538378774	39.0	35.0	41.0	27.0	41.0
21	36.18817563871702	39.0	35.0	41.0	27.0	41.0
22	35.94202604881758	39.0	34.0	41.0	27.0	41.0
23	35.62993451837283	39.0	34.0	41.0	25.0	41.0
24	35.95449011684932	39.0	34.0	41.0	27.0	41.0
25	34.683066628779635	38.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26589434110750787
1101	2	0.5372814795738954
1101	3	0.26403585439962285
1101	4	0.3409849686273816
1101	5	0.21950265218208642
1101	6	0.2570829781816144
1101	7	0.18823906149791014
1101	8	0.24698436973453397
1101	9	0.21291496616622396
1101	10	0.5218059109600972
1101	11	0.4391011457289977
1101	12	0.43286067155195695
1101	13	0.44028772495757096
1101	14	0.9070847342198718
1101	15	0.6188606835825539
1101	16	0.40712737680633637
1101	17	0.6578171526277927
1101	18	0.7568591367561197
1101	19	0.7053018101347703
1101	20	0.7990063704979775
1101	21	0.8687727365783715
1101	22	0.6712335789658397
1101	23	-1.5929257778046306
1101	24	0.10203915772665795
1101	25	0.4307688906879932
1102	1	0.3464553492040494
1102	2	0.47782703477792055
1102	3	0.3464195119972331
1102	4	0.4046978955021743
1102	5	0.23896065565659086
1102	6	0.23098512860098452
1102	7	0.20290036272112388
1102	8	0.27727412594757794
1102	9	0.44700035158513884
1102	10	0.9625539883226111
1102	11	0.599772760429957
1102	12	0.4999402640832713
1102	13	0.5395446011876999
1102	14	1.1079824967506724
1102	15	0.7929302885986971
1102	16	0.6194866753292914
1102	17	0.9801003514464384
1102	18	0.962472148339252
1102	19	0.902716206517141
1102	20	0.9775630907064183
1102	21	0.9677498311799582
1102	22	0.82563409615247
1102	23	0.8524137836820955
1102	24	0.8789455108115689
1102	25	0.7876798588717193
1103	1	-0.32288813334916
1103	2	-1.5905820910799662
1103	3	-0.39421796464189995
1103	4	0.01721457021955075
1103	5	-0.09983863674862192
1103	6	-0.024544074494215806
1103	7	-0.02950027593442428
1103	8	0.13538628170466183
1103	9	0.741771697259459
1103	10	0.6812167349215557
1103	11	0.5247523707001918
1103	12	0.3582170536534903
1103	13	0.25230394211379803
1103	14	-0.8531391946775031
1103	15	-0.06393253170781321
1103	16	0.012983690780330903
1103	17	0.4562329507718985
1103	18	0.6756054028938507
1103	19	0.6715136181994339
1103	20	0.6779449524522363
1103	21	0.6977266703607299
1103	22	0.24854849955218583
1103	23	1.12740230030564
1103	24	0.9123611485079479
1103	25	0.6519764670200487
1104	1	-0.0545348602747282
1104	2	0.0665553276926012
1104	3	-0.33796916457402304
1104	4	-0.26645481014101335
1104	5	-0.2767352423800773
1104	6	-0.16092583785294323
1104	7	-0.15115046513761854
1104	8	-0.051560917710617105
1104	9	-2.303329093411584
1104	10	-0.6135105334987685
1104	11	-0.47865505288172727
1104	12	-0.04989011116300901
1104	13	0.06615787395288208
1104	14	-1.7615871604450106
1104	15	-0.6335073618395555
1104	16	-0.24371393586728374
1104	17	0.1380009494886636
1104	18	0.2595358086862305
1104	19	0.2908767203867839
1104	20	0.3801417284018598
1104	21	0.5253967053983999
1104	22	0.7089581264194393
1104	23	1.1034885058566672
1104	24	0.8216951772171441
1104	25	0.6094889161109052
1105	1	-0.29935852474756786
1105	2	-0.13599932404448722
1105	3	-1.4350499139983555
1105	4	-0.5685054002154857
1105	5	-0.512951131243014
1105	6	-0.15887137531453277
1105	7	-0.09071480672118781
1105	8	-0.0965430636493565
1105	9	0.24822821755270752
1105	10	0.49233370193004333
1105	11	-0.1324549372057362
1105	12	-0.23495321405875558
1105	13	-0.32300158677823987
1105	14	0.5254693088199005
1105	15	0.17560232947636223
1105	16	-0.29619388639499533
1105	17	0.2338359075113985
1105	18	0.1349587522421345
1105	19	0.17833344709071497
1105	20	-0.43535641889070575
1105	21	0.011130653812145397
1105	22	0.13789810206485953
1105	23	0.3170607910517589
1105	24	0.19889456962728502
1105	25	0.44794863668138163
1106	1	-1.4824337796281242
1106	2	-0.9757583925579318
1106	3	-0.9820738011338825
1106	4	-1.3285840871670729
1106	5	-0.9028747792638327
1106	6	-0.6856955588959224
1106	7	-0.5832069802968292
1106	8	-0.6159475376918948
1106	9	-0.2197262945364855
1106	10	-1.7164081228342596
1106	11	-1.0653853193340481
1106	12	-0.7032982712488121
1106	13	-0.8683565350997213
1106	14	-0.13617753500511043
1106	15	-0.9150442124648208
1106	16	-1.3387490600861938
1106	17	-0.612304330136709
1106	18	-0.5004299953566473
1106	19	-0.46591318826113337
1106	20	-0.16885614585930142
1106	21	-0.18957024399136202
1106	22	-0.15462465126280023
1106	23	-0.47384738844632324
1106	24	-0.6758092809820369
1106	25	-1.2566887421042026
1107	1	0.11809993624842363
1107	2	0.25961182216732226
1107	3	0.05737653528248643
1107	4	0.2575277621387144
1107	5	-0.012106439974878924
1107	6	0.08261597964304457
1107	7	0.016627435919680522
1107	8	-0.07033116435234632
1107	9	0.516638436171668
1107	10	-0.0014543102527895257
1107	11	0.008117992274065955
1107	12	-0.040911284275154
1107	13	0.20508230475457623
1107	14	-1.1078050725502422
1107	15	-0.36461801931517357
1107	16	-0.013390462976424544
1107	17	0.08099878127391236
1107	18	0.41569527925965133
1107	19	0.33480707958036504
1107	20	0.40473418640395664
1107	21	0.5643210492279849
1107	22	-0.18842792675754794
1107	23	0.5801725375408608
1107	24	0.5066872323147251
1107	25	0.24755951684095123
1108	1	-0.2645946495188838
1108	2	0.051551176244572616
1108	3	-0.11707128733442929
1108	4	-0.47303302893424615
1108	5	-0.3967388893217887
1108	6	-0.33072778717379947
1108	7	-0.2333584823438528
1108	8	-0.31423158437804943
1108	9	0.24677818332434498
1108	10	0.2961428039011338
1108	11	0.03263139245448343
1108	12	-0.0055414068859178656
1108	13	-0.20527115116352235
1108	14	-0.053087907476765395
1108	15	-0.1316167983203016
1108	16	-0.19916764917684304
1108	17	0.15938071609583915
1108	18	0.1933634866849303
1108	19	0.1161914789228149
1108	20	0.22388348487351095
1108	21	0.21840379957742329
1108	22	0.07144460536397901
1108	23	-0.03272862946680277
1108	24	0.03588161833801706
1108	25	-0.8813142388751487
1109	1	-0.9127170889661329
1109	2	-0.7036935901185437
1109	3	-0.8837924663594983
1109	4	-0.6676375553104918
1109	5	-1.0062888998064636
1109	6	-1.219354043943575
1109	7	-1.0280330449333022
1109	8	-1.3593535563872337
1109	9	-0.9098749786927627
1109	10	-1.1859603555809244
1109	11	-1.3547340534851386
1109	12	-1.1700313552893107
1109	13	-1.4066795865656516
1109	14	-0.7610190117700384
1109	15	-1.3116782772017643
1109	16	-1.4947520907323337
1109	17	-1.4308886667286913
1109	18	-2.0671699677300523
1109	19	-2.277090322278923
1109	20	-1.9077359707254402
1109	21	-1.6219595213385887
1109	22	-1.8217425069481763
1109	23	-1.3010824631213893
1109	24	-2.1539721226592548
1109	25	-1.280528736238793
1110	1	0.11242826899558267
1110	2	0.21481593324974213
1110	3	0.004969582056798316
1110	4	0.2564651716566928
1110	5	0.17503404187146288
1110	6	0.19772511892605849
1110	7	0.09472444783672529
1110	8	0.03431378390274631
1110	9	0.3561052480380056
1110	10	0.2682786867220912
1110	11	0.29526349846511124
1110	12	0.2630283861979379
1110	13	0.16802974894053335
1110	14	-2.3766829961638507
1110	15	-0.5994042451325967
1110	16	-0.1404367442018497
1110	17	-0.16601109097920386
1110	18	-2.759484457908698
1110	19	-0.5929276537145611
1110	20	-1.9072019039314227
1110	21	-0.24688767957775326
1110	22	-1.8953541611981422
1110	23	-0.5580768639194744
1110	24	-0.041810430196541404
1110	25	-0.02169908851780633
1111	1	-0.2072663363428333
1111	2	-1.376782685209438
1111	3	-0.4595978467592339
1111	4	0.004886196757837524
1111	5	0.0257377964695209
1111	6	0.12618659819870715
1111	7	-0.03989217326940775
1111	8	-0.0904554816677674
1111	9	-1.6254739616674883
1111	10	-0.3301777419136158
1111	11	-0.20548308490378275
1111	12	-0.17063374093356032
1111	13	-0.07233426638546092
1111	14	0.7326676525598117
1111	15	0.22992236992462267
1111	16	0.19847515864142196
1111	17	-0.2094985971812804
1111	18	0.1524779747846381
1111	19	-0.13087706479377914
1111	20	0.3093044003076386
1111	21	-0.535954701432189
1111	22	-0.28859399627012294
1111	23	-0.06565516340510413
1111	24	-0.5310543410489359
1111	25	-1.9177871560493926
1112	1	0.20335316666874093
1112	2	0.32751201396259333
1112	3	-0.04909877165994203
1112	4	0.3493874296002488
1112	5	0.21447213775353902
1112	6	0.17235679936948856
1112	7	0.19775030155565787
1112	8	0.0671580442471793
1112	9	-0.36843466535849245
1112	10	0.42286378990074525
1112	11	0.20036451685979273
1112	12	0.09251335791699944
1112	13	0.08931052712508603
1112	14	0.5354897928430731
1112	15	0.2076943969785603
1112	16	0.21664112957762427
1112	17	-3.7151576135350126
1112	18	-0.9804970479962734
1112	19	-1.1865785595738672
1112	20	-0.03800792595727387
1112	21	-0.4745415254951766
1112	22	-1.7463846304921873
1112	23	-1.5061215672045591
1112	24	-0.35214748309562793
1112	25	0.08282884686414604
1113	1	-0.29095877946754456
1113	2	-0.1303580895269114
1113	3	-0.09884016868121748
1113	4	-0.3652210255587818
1113	5	-0.12141075389732237
1113	6	-0.18515563575928695
1113	7	-0.2781449007979191
1113	8	-0.21284418430213492
1113	9	0.2444558896338762
1113	10	0.4389039576232392
1113	11	-0.07622134406078374
1113	12	-0.133873241093589
1113	13	-0.38829759451842705
1113	14	-1.513518391515774
1113	15	-1.7160789403844703
1113	16	-0.4927202652764535
1113	17	-0.25103881038409526
1113	18	-0.6214530795465834
1113	19	-0.22455847318192212
1113	20	0.20339210531604124
1113	21	-1.6100666567598765
1113	22	-1.9417279823791276
1113	23	-0.6000448937584295
1113	24	-2.326600819412633
1113	25	-0.6280229766160375
1114	1	0.15541748757257068
1114	2	0.2643578875350343
1114	3	-0.002023849228724117
1114	4	0.4079703972542532
1114	5	0.23068951475042354
1114	6	0.22477308008161145
1114	7	0.22301811417694495
1114	8	0.11181788203990806
1114	9	0.553446737278577
1114	10	0.35667971749862204
1114	11	0.2847935003281634
1114	12	0.22035406119618983
1114	13	0.1890448814410135
1114	14	0.9693195926241316
1114	15	0.48926103239843144
1114	16	0.3821084170014615
1114	17	-0.38687217146960506
1114	18	-0.007813269199949957
1114	19	-0.427554335373344
1114	20	-1.667139574981725
1114	21	-0.7368283244228593
1114	22	-1.4284763195290893
1114	23	-2.8188745547580467
1114	24	-1.9923288464666626
1114	25	-1.3992333036959437
1115	1	0.45283711956884076
1115	2	0.518578711240469
1115	3	0.3717154038126189
1115	4	0.6429975461917294
1115	5	0.6196808007261794
1115	6	0.426604104859031
1115	7	0.5431435211724818
1115	8	0.4793788462195323
1115	9	0.7258442441378108
1115	10	0.9243033441353319
1115	11	0.6225913583412961
1115	12	0.4796076301044394
1115	13	0.47048421885778424
1115	14	1.0867594341748301
1115	15	0.6099096765230314
1115	16	0.7129496864451852
1115	17	0.5970720822350515
1115	18	0.8142278957969964
1115	19	0.21053623039806268
1115	20	0.8572639863953881
1115	21	-0.26041714056333376
1115	22	0.80632973588677
1115	23	0.8730789424307375
1115	24	0.8907783281531607
1115	25	0.961612071920662
1116	1	0.40080403517616503
1116	2	0.4309455942632141
1116	3	0.39264947665019534
1116	4	0.6476197238632864
1116	5	0.6067369911747562
1116	6	0.3808319539040639
1116	7	0.3741610022465025
1116	8	0.30152476161836717
1116	9	0.5264115747842979
1116	10	0.5257025480222808
1116	11	0.4050255091885617
1116	12	0.36850056905392137
1116	13	-0.07753288528445523
1116	14	0.8458744867312689
1116	15	0.5662565586897372
1116	16	0.5663707219996539
1116	17	0.7120764521583851
1116	18	0.6998203345179661
1116	19	0.9048119760119206
1116	20	0.6705944616745967
1116	21	0.11892161340790608
1116	22	0.1910236134377925
1116	23	-0.596035364546367
1116	24	-0.11959689155111874
1116	25	-0.6754976207487786
1201	1	0.4137020088653216
1201	2	0.6989156469303524
1201	3	0.52634652799291
1201	4	0.4151501949748777
1201	5	0.4054736203538738
1201	6	0.31135668307843645
1201	7	0.35188139738434643
1201	8	0.40210629030842426
1201	9	0.7060423676013983
1201	10	1.0066763632182685
1201	11	0.60151960092319
1201	12	0.6643493901902033
1201	13	0.5822408051137558
1201	14	1.1902929890340346
1201	15	0.917889054045979
1201	16	0.5795136423088394
1201	17	0.7224195840452481
1201	18	0.6057521544372619
1201	19	0.9491672529534654
1201	20	0.8801170068879642
1201	21	0.9656771812687666
1201	22	1.0140854771809416
1201	23	0.620675759604687
1201	24	0.9937736193965776
1201	25	1.102435385162586
1202	1	0.3944619513981138
1202	2	0.7747495225234502
1202	3	0.5005631010474261
1202	4	0.4510340065184337
1202	5	0.2867175665475017
1202	6	0.24216505606223393
1202	7	0.26146874637529294
1202	8	0.32023459722072545
1202	9	0.7794540061275939
1202	10	0.07960922533820991
1202	11	0.3830930396506744
1202	12	0.36106683727427935
1202	13	0.3450950315245791
1202	14	0.9721060541369226
1202	15	0.6458613969804574
1202	16	0.47053641546391844
1202	17	-1.617818129430752
1202	18	-0.028827801811743825
1202	19	0.5609703937938804
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2210	19	0.6776630995651658
2210	20	0.831368403181834
2210	21	0.8592190263321555
2210	22	1.010379371704616
2210	23	1.394163141394067
2210	24	1.0100758805220593
2210	25	0.9674668960887303
2211	1	0.09264192730872978
2211	2	-0.6380389340072874
2211	3	-0.08384859328814898
2211	4	0.27702767646246684
2211	5	0.3270999225502109
2211	6	0.21220844896328117
2211	7	0.1673015777989022
2211	8	0.2982955541422925
2211	9	0.6559811091270475
2211	10	0.7501965301513849
2211	11	0.42323631926500127
2211	12	0.36448667306160587
2211	13	0.18934835185175558
2211	14	-0.8241433028786531
2211	15	-0.015415194217212047
2211	16	0.28944021814385934
2211	17	0.35786108234186287
2211	18	0.2726403546220695
2211	19	0.5625193329771676
2211	20	0.6716683301413795
2211	21	0.5683039071197484
2211	22	-0.020593844842053954
2211	23	0.4424005422401365
2211	24	-0.17149603684447356
2211	25	0.29714395756257517
2212	1	0.06502661351996863
2212	2	-1.0471143228076194
2212	3	-0.30576799436459723
2212	4	0.17837109453970612
2212	5	0.18803444065177644
2212	6	0.0550951822237451
2212	7	0.06909007483308471
2212	8	0.13449012767819823
2212	9	-2.0137626232206856
2212	10	0.003973130451541351
2212	11	0.049059354457831716
2212	12	-0.10631284762988713
2212	13	-0.016331384070035426
2212	14	0.6238431714919486
2212	15	0.3038972929755914
2212	16	0.23536969077318304
2212	17	0.36311814431956435
2212	18	0.050919450980075
2212	19	-0.5998808532928877
2212	20	0.35120899301814745
2212	21	0.3188181254661018
2212	22	-1.6301656431038083
2212	23	-0.3564897836422105
2212	24	0.22882563879298345
2212	25	-0.3611150433985699
2213	1	0.17124031010250462
2213	2	0.4435204086842326
2213	3	0.13295169023470166
2213	4	0.32610991964266844
2213	5	0.3690289615111695
2213	6	0.26906772436641546
2213	7	0.17901721257879188
2213	8	0.22307672554912017
2213	9	0.5869729997752344
2213	10	0.5799620949633635
2213	11	-0.02782318720124266
2213	12	0.11787921263696433
2213	13	0.06224609034715911
2213	14	-1.7643217719848252
2213	15	-0.40683926404919646
2213	16	-0.08100484512288375
2213	17	-2.0864214129842864
2213	18	-0.7693443948355778
2213	19	-0.45588391702951725
2213	20	0.2048697250003002
2213	21	-0.22675743390610847
2213	22	0.31806737892086545
2213	23	0.8448071179161332
2213	24	0.5791696918129006
2213	25	-0.929353073586185
2214	1	0.29246171680200916
2214	2	0.24435678536459093
2214	3	0.15591107832604933
2214	4	0.46432850164051587
2214	5	0.5389823617253882
2214	6	0.4183974125517551
2214	7	0.38441870950006773
2214	8	0.27332871239332235
2214	9	0.6819690378665726
2214	10	0.6448039424675827
2214	11	0.002818551899778754
2214	12	0.02701424625650617
2214	13	0.18181740954051406
2214	14	0.9984542848669591
2214	15	0.5861027852911675
2214	16	0.5688177090788997
2214	17	0.4603193604656539
2214	18	0.5204767014842631
2214	19	-0.7890782198277506
2214	20	0.20109898794053294
2214	21	0.592848877408116
2214	22	0.732330294414453
2214	23	1.2939678424583505
2214	24	0.9805699010123092
2214	25	0.9868053838535928
2215	1	-0.10941863904810134
2215	2	0.2473648846358074
2215	3	0.11489342973996841
2215	4	0.34699031891766197
2215	5	0.42838709892034643
2215	6	0.21368831444997483
2215	7	0.15094591644118793
2215	8	0.16947738200539675
2215	9	0.37382959212315825
2215	10	0.7268802820222362
2215	11	0.33929998664022065
2215	12	0.24865475548580207
2215	13	0.10001335997722549
2215	14	0.9928675668328282
2215	15	0.4512685422754288
2215	16	0.4698580521136577
2215	17	0.6608275989555281
2215	18	0.45833401429664633
2215	19	-0.22691181122787185
2215	20	-1.5153562953893172
2215	21	-1.8402207601243674
2215	22	-0.1976556318452225
2215	23	0.5391028556244564
2215	24	0.6103531827684492
2215	25	-0.1578650367747798
2216	1	0.002450362120164584
2216	2	-0.29862762215364214
2216	3	-0.08705393961880503
2216	4	0.24083947224086444
2216	5	0.4224252369186132
2216	6	0.0788047977315145
2216	7	0.16732357018053534
2216	8	-0.03151195595010847
2216	9	0.21577617245500846
2216	10	-1.683433954314232
2216	11	-0.522671715290862
2216	12	-0.21356281741647365
2216	13	0.0016393917571946304
2216	14	-0.49389854267901967
2216	15	-0.48598549130657887
2216	16	-0.015797183843567097
2216	17	0.3483880804146722
2216	18	0.1428727771533289
2216	19	-1.321571753348934
2216	20	-0.9911349504292915
2216	21	-0.8822205361425333
2216	22	-0.7044235739357418
2216	23	-4.523451580870326
2216	24	-2.144374870193076
2216	25	-1.3087034415150498
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	27.0
4	52.0
5	70.0
6	84.0
7	362.0
8	173.0
9	484.0
10	1272.0
11	3037.0
12	5880.0
13	9825.0
14	14282.0
15	18750.0
16	22144.0
17	24325.0
18	25999.0
19	27387.0
20	27811.0
21	28864.0
22	30054.0
23	32054.0
24	34752.0
25	38639.0
26	43572.0
27	50017.0
28	58528.0
29	72668.0
30	88919.0
31	110200.0
32	145739.0
33	194698.0
34	261614.0
35	363593.0
36	513156.0
37	762742.0
38	1257054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0287364513957	21.5869661441971	13.476488869466383	22.907808534940816
2	15.45653723874979	21.261545548287753	41.251156575687794	22.030760637274668
3	17.79358888557701	26.684396165966483	30.709175285368545	24.81283966308796
4	12.114440918826954	17.59079766965537	39.7573807526282	30.53738065888948
5	12.748804258358943	37.990882177027885	36.15756790556313	13.102745659050044
6	29.87544906307396	39.52964033014585	18.24712579717262	12.347784809607566
7	25.187851801102145	33.11116885539096	24.72844112235198	16.972538221154917
8	22.690453135560094	38.11579558141039	21.837396689760418	17.3563545932691
9	23.918807320530032	17.220647951646406	22.99446104641135	35.86608368141221
10	14.045700609667943	30.475617851444785	35.23812290975091	20.24055862913636
11	31.159731347368304	26.068415320623007	24.66010718362741	18.11174614838128
12	20.098255827847993	27.98547905573563	32.634050930145484	19.282214186270892
13	25.767262318444615	24.724032067813482	27.719739832125267	21.788965781616636
14	20.334295652736355	24.020574996584347	29.037845423178766	26.60728392750053
15	22.00803000154432	31.867079641745605	24.892231455080992	21.232658901629083
16	21.239849185556547	31.922968507374243	26.49562616088389	20.34155614618532
17	19.36728979035798	30.964977497768757	29.23450706089954	20.43322565097372
18	19.457632361159767	30.64693497610475	30.199820273058414	19.695612389677066
19	20.15613870665417	29.655623242736645	30.29960168697282	19.888636363636365
20	21.400601622774225	29.404072937916737	29.578294721122145	19.617030718186893
21	21.793815099621252	29.67699295217308	28.554499649272092	19.974692298933572
22	20.61190147382509	29.34449437222905	30.077564315541306	19.96603983840455
23	21.057272087402072	29.498105394961655	29.289455161638912	20.155167355997364
24	20.6192033304842	28.856847099134765	29.674355571550677	20.849593998830358
25	21.289034833860693	29.199425329947477	29.054233430862762	20.457306405329064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2500.0
1	2500.0
2	3172.5
3	3845.0
4	3845.0
5	3845.0
6	6073.0
7	8301.0
8	8301.0
9	8301.0
10	14055.5
11	19810.0
12	19810.0
13	19810.0
14	34169.0
15	48528.0
16	48528.0
17	48528.0
18	75737.0
19	102946.0
20	102946.0
21	102946.0
22	145283.0
23	187620.0
24	187620.0
25	187620.0
26	238630.5
27	289641.0
28	289641.0
29	289641.0
30	343341.0
31	397041.0
32	397041.0
33	397041.0
34	439198.0
35	481355.0
36	481355.0
37	481355.0
38	505287.0
39	529219.0
40	529219.0
41	529219.0
42	525024.5
43	520830.0
44	520830.0
45	520830.0
46	494035.5
47	467241.0
48	467241.0
49	467241.0
50	437406.5
51	407572.0
52	407572.0
53	407572.0
54	364470.0
55	321368.0
56	321368.0
57	321368.0
58	274668.5
59	227969.0
60	227969.0
61	227969.0
62	183658.5
63	139348.0
64	139348.0
65	139348.0
66	104426.0
67	69504.0
68	69504.0
69	69504.0
70	49167.0
71	28830.0
72	28830.0
73	28830.0
74	19664.0
75	10498.0
76	10498.0
77	10498.0
78	6976.0
79	3454.0
80	3454.0
81	3454.0
82	2245.0
83	1036.0
84	1036.0
85	1036.0
86	653.5
87	271.0
88	271.0
89	271.0
90	173.0
91	75.0
92	75.0
93	75.0
94	45.0
95	15.0
96	15.0
97	15.0
98	14.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08074810176369128
2	0.01977122073935464
3	0.03202281842499738
4	0.03867569365008828
5	0.04739002317027777
6	0.018225130018030698
7	0.022769699714043495
8	0.03717645416274385
9	0.03745756156662093
10	0.02572132745475283
11	0.02377700124460303
12	0.018482811804918023
13	0.026353819113476264
14	0.04005780505248393
15	0.03710617731177458
16	0.025065410179039647
17	0.024175236733428893
18	0.029188318769236825
19	0.0194666877184878
20	0.01658533682874773
21	0.013235473599212524
22	0.020825373503893692
23	0.021621844481545416
24	0.020216307462160014
25	0.11288804827363744
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4268831.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.03889329850298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.65056866193811	69.35415619387472
2	10.417740100617777	16.67648776649764
3	1.8029045674306285	4.329074588998913
4	0.4624877379518174	1.480680268391661
5	0.19674136397948686	0.7873480519478042
6	0.10400471646225477	0.49946534420780797
7	0.06354481321458164	0.3560239567188641
8	0.044786120460386616	0.2867705213426228
9	0.03364557387691691	0.24236590477513076
>10	0.19918922267595807	2.965765643788747
>50	0.01601319588745831	0.888250207032347
>100	0.007463906947872784	1.0698410399854241
>500	4.990426440955194E-4	0.2534489087947895
>1k	3.816204902063501E-4	0.6919264934383439
>5k	2.9355422323563425E-5	0.11839511020529368
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5039	0.1180416840113839	No Hit
GTGGTATCAACGCAGAGTACATGGG	4609	0.10796866870578854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.342561698975668E-5
3	0.0	0.0	0.0	2.342561698975668E-5	2.342561698975668E-5
4	0.0	0.0	0.0	4.685123397951336E-5	2.342561698975668E-5
5	0.0	0.0	0.0	4.685123397951336E-5	2.342561698975668E-5
6	0.0	0.0	0.0	4.685123397951336E-5	2.342561698975668E-5
7	0.0	0.0	0.0	1.1712808494878341E-4	2.342561698975668E-5
8	4.685123397951336E-5	0.0	0.0	1.1712808494878341E-4	2.342561698975668E-5
9	4.685123397951336E-5	0.0	0.0	1.4055370193854008E-4	2.342561698975668E-5
10	4.685123397951336E-5	0.0	0.0	1.4055370193854008E-4	2.342561698975668E-5
11	4.685123397951336E-5	0.0	0.0	1.6397931892829677E-4	2.342561698975668E-5
12	4.685123397951336E-5	0.0	0.0	1.6397931892829677E-4	2.342561698975668E-5
13	4.685123397951336E-5	2.342561698975668E-5	0.0	1.6397931892829677E-4	2.342561698975668E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACT	85	5.313068E-5	11.179484	4
TGTACTG	580	0.0	9.338724	5
CTAGGAC	400	0.0	9.2649975	3
GTCTAGA	480	0.0	9.111428	1
TAACCCG	105	4.4834398E-4	9.050058	5
GGTATCA	3505	0.0	9.032876	1
CTAGACT	415	0.0	8.930119	4
GTGTTAC	390	0.0	8.776226	1
TATTCCG	130	4.3585926E-5	8.771596	5
TAGGACA	555	0.0	8.732083	4
TAGACTG	575	0.0	8.59362	5
GTAGGAC	310	0.0	8.582958	3
TCTAGAC	435	0.0	8.519538	3
TCTACAC	565	0.0	8.409347	3
GTCTTAC	385	0.0	8.396302	1
GTCTCGC	295	1.8189894E-12	8.37956	1
CCGTGCA	125	2.7504298E-4	8.35853	9
TAGACAG	595	0.0	8.30476	5
TATACTG	1135	0.0	8.288578	5
GTATTAA	805	0.0	8.267458	1
>>END_MODULE