FastQCFastQC Report
Wed 25 May 2016
SRR1295320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4268831
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT139720.32730272058088034No Hit
GGTATCAACGCAGAGTACTTTTTTT116110.2719948388680648No Hit
TATCAACGCAGAGTACTTTTTTTTT84860.19878978577507517No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59300.1389139087492571No Hit
GTGGTATCAACGCAGAGTACATGGG52120.12209431575061182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3200.014.5586823
CAGCGTC3700.012.8482572
GCAGCGT3700.012.3496781
AGACCGA701.0958033E-412.2094526
ACTCGCG550.003079340312.0859818
GGTATCA45800.010.1430951
GTCGTAG856.4744725E-410.0795171
TGTGCGA951.6521312E-49.99592510
GTCCTAC2550.09.70620251
CTAGGAC3850.09.6311873
GCGTCAG5000.09.3174484
GTCCTAG5250.09.0662331
ACCGAGC850.00746410538.9375338
GTCTCGC2451.6370905E-118.9367151
CTACACT5900.08.8630164
TAGACAG7300.08.7261525
CTAGAAC4700.08.6985443
GCCTAAG3850.08.6541321
GTCTTAG4300.08.6340051
ATGTGCG1107.177813E-48.6328449
Produced by FastQC (version 0.11.5)