FastQCFastQC Report
Wed 25 May 2016
SRR1295319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7546272
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA367130.4865051246496283No Hit
GCTTATGTACTCTGCGTTGATACCA250480.3319254858557974No Hit
ATTCCATTCCATTCCATTCCATTCC121690.16125843330322576No Hit
CTTATGTACTCTGCGTTGATACCAC116940.15496393450964926No Hit
GAGTACATAAGCAGTGGTATCAACG94660.1254394222736737No Hit
GAATGGAATGGAATGGAATGGAATG92410.12245781758197956No Hit
TATGTACTCTGCGTTGATACCACTG79490.10533678086345152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG4950.010.1735285
GCTTATG61050.09.7167631
ACATAAG93000.09.3993973
CATAAGC93450.09.2627514
GTACATA104000.09.2048951
AGTACCG1054.4885627E-49.049235
CTAGACT7900.09.0205924
TACATAA101600.08.9963812
TCGGAAC1606.765913E-78.90500113
TATACTG18050.08.7383975
GACCGTC1201.7683776E-48.7070547
ACAGTCG1000.0029102558.548978
GTACCGT1451.6944306E-58.515996
AGTGGTA109200.08.36787411
GTCTATA8650.08.35257151
GTATAGA13350.08.3315791
TAGACAG11650.08.3190775
TACACCG1502.5834208E-58.2347985
TAAGCAG108850.08.1940196
ATAAGCA110700.08.1712835
Produced by FastQC (version 0.11.5)