FastQCFastQC Report
Wed 25 May 2016
SRR1295319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7546272
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA392880.5206279338990166No Hit
GCTTATGTACTCTGCGTTGATACCA274880.3642593322901692No Hit
GTATCAACGCAGAGTACTTTTTTTT173140.22943779391996472No Hit
CTTATGTACTCTGCGTTGATACCAC135870.1800491686491025No Hit
GGTATCAACGCAGAGTACTTTTTTT120570.15977425674558246No Hit
GAATGGAATGGAATGGAATGGAATG111360.14756955487424786No Hit
ATTCCATTCCATTCCATTCCATTCC106300.14086425721203794No Hit
GAGTACATAAGCAGTGGTATCAACG103240.13680927483133393No Hit
TATCAACGCAGAGTACTTTTTTTTT99430.1317604242200652No Hit
GTGGTATCAACGCAGAGTACATGGG82980.10996158102967929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3300.010.9467793
GCTTATG62100.010.1311071
AGTACCG1356.3952393E-79.8584385
TATTCCG3400.09.50635055
TAGCGTC900.00111816159.4966517
CTAGGAC7600.09.0060763
CGTAGCG850.00746144268.93808415
GTCCTAG7600.08.8918431
GTACATA103150.08.7198621
ACATAAG96200.08.666453
CATAAGC96050.08.660194
TAGACTG10550.08.6503285
CTTACAC11250.08.6191483
TAGACAG11950.08.5915145
ACCGAGC1000.00291637848.5469868
CAGCGTC4350.08.5229912
GTCTTAC5600.08.498241
TACATAA103850.08.4491282
GTTCTAG8600.08.4112831
ACACCGC1701.6220529E-68.3795096
Produced by FastQC (version 0.11.5)