FastQCFastQC Report
Wed 25 May 2016
SRR1295318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415542
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG68510.15515649041499324No Hit
GTATCAACGCAGAGTACTTTTTTTT66130.14976643863879No Hit
GGTATCAACGCAGAGTACTTTTTTT52460.11880761184017727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG1051.9905201E-812.6696355
CGCAGAT1752.3646862E-1010.3126138
GGTATCA49000.09.6252351
TGGACCG900.00111198199.5022285
TAGGACC2650.09.3229394
GCGTCAG1606.6596294E-78.91451
TAGACTG5650.08.7454125
CAATACG1000.00290043338.5520054
GTCTTAG4150.08.4777261
CGTCAGA1804.026715E-78.4461362
CTTAGAC3650.08.3306253
GGAGCGT1150.00111304658.259536
GTAGGAC3150.08.1446733
GTGTAGA6800.08.1104471
TAGGACA4950.08.0624954
GTCCTGG8150.08.0503951
CGCAAAA1304.1794722E-48.0400722
TTAGACT4150.08.01392654
CTTACAC5700.08.0017843
GTCTAGA5000.07.9873921
Produced by FastQC (version 0.11.5)