##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295318_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4415542
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.19513233030056	31.0	30.0	33.0	26.0	34.0
2	30.04660379178819	31.0	30.0	34.0	25.0	34.0
3	30.49381819944188	31.0	30.0	34.0	26.0	34.0
4	33.883060109042106	35.0	35.0	37.0	28.0	37.0
5	34.215802725916774	35.0	35.0	37.0	30.0	37.0
6	34.309675686472914	37.0	35.0	37.0	30.0	37.0
7	34.28104364084862	37.0	35.0	37.0	30.0	37.0
8	34.22381986175196	37.0	35.0	37.0	30.0	37.0
9	35.27869217414306	39.0	35.0	39.0	28.0	39.0
10	35.21608513745311	39.0	35.0	39.0	27.0	39.0
11	35.573143002603075	39.0	35.0	39.0	29.0	39.0
12	35.58168396994072	39.0	35.0	39.0	29.0	39.0
13	35.481857493372274	39.0	35.0	39.0	28.0	39.0
14	35.72347132016862	39.0	34.0	41.0	25.0	41.0
15	36.328327530346215	39.0	36.0	41.0	27.0	41.0
16	36.43831380156728	39.0	36.0	41.0	27.0	41.0
17	36.261648286892076	39.0	36.0	41.0	27.0	41.0
18	36.24339412919184	39.0	36.0	41.0	27.0	41.0
19	36.300577596136556	39.0	36.0	41.0	27.0	41.0
20	36.26579794734146	39.0	36.0	41.0	27.0	41.0
21	36.26916718264712	39.0	35.0	41.0	27.0	41.0
22	36.02891694836104	39.0	35.0	41.0	27.0	41.0
23	35.714394744744816	39.0	34.0	41.0	26.0	41.0
24	36.0301439777948	39.0	34.0	41.0	27.0	41.0
25	34.77887946711865	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4332976594346398
1101	2	0.674878013660706
1101	3	0.5467636912185263
1101	4	0.5689296421130052
1101	5	0.5007806317330576
1101	6	0.3935338887870401
1101	7	0.42484792471977784
1101	8	0.4164000044014813
1101	9	0.38575816596561197
1101	10	0.6952267562915324
1101	11	0.6432467765959302
1101	12	0.6212301857728377
1101	13	0.707415432449082
1101	14	1.2307321905384825
1101	15	0.9422325856506291
1101	16	0.6595705365667897
1101	17	0.9737813292373474
1101	18	1.0015573767271277
1101	19	0.9975418881412494
1101	20	1.1173124161065076
1101	21	1.081401828424454
1101	22	0.9247705259995698
1101	23	-1.4449054335900584
1101	24	0.36038900386197525
1101	25	0.7431954235548588
1102	1	0.16446171349913286
1102	2	0.25660161776074375
1102	3	0.28954970443365724
1102	4	0.40712336428208573
1102	5	0.29613905101160043
1102	6	0.25556890342083705
1102	7	0.19602317291669635
1102	8	0.1939515932758269
1102	9	0.31782114331254974
1102	10	0.8192853722068278
1102	11	0.4970761389757854
1102	12	0.42767082224817443
1102	13	0.43139590343776746
1102	14	0.9447444205718156
1102	15	0.6092916587959536
1102	16	0.5114368806438705
1102	17	0.7212339420772977
1102	18	0.7558676570606337
1102	19	0.7262667296157019
1102	20	0.8443494385160051
1102	21	0.8279664702765928
1102	22	0.6239413094183348
1102	23	0.7254755601162444
1102	24	0.7960795482127665
1102	25	0.5605452501101738
1103	1	-0.3506714280909158
1103	2	-1.5822639088755004
1103	3	-0.4026269991646245
1103	4	-0.08971183882155742
1103	5	-0.09676390496984766
1103	6	-0.090570968327782
1103	7	-0.03269442284677382
1103	8	0.1107892910773316
1103	9	0.6778607577376974
1103	10	0.6596592217946622
1103	11	0.42480345563654964
1103	12	0.307296305958225
1103	13	0.0680487309024187
1103	14	-1.0599999892985252
1103	15	-0.2913885675892729
1103	16	-0.1303458844361245
1103	17	0.33053794220846555
1103	18	0.4933068669171732
1103	19	0.4955593088497636
1103	20	0.5022986881726652
1103	21	0.5605220418454806
1103	22	0.17194051010047673
1103	23	0.9674173191572351
1103	24	0.698454112874856
1103	25	0.6901063508679783
1104	1	-0.1413120857689698
1104	2	-0.07383074533451861
1104	3	-0.3710539529910797
1104	4	-0.18644787223526293
1104	5	-0.22079217604871104
1104	6	-0.2657234188764619
1104	7	-0.1546852745281484
1104	8	-0.2293576634391954
1104	9	-2.143598518629993
1104	10	-0.715757129933067
1104	11	-0.5476716177268628
1104	12	-0.17740812271087236
1104	13	-0.04210062717851315
1104	14	-1.9834627097622146
1104	15	-0.7314248721181258
1104	16	-0.42554190677781634
1104	17	0.05102860222650207
1104	18	0.10164761408487522
1104	19	0.08198046971666884
1104	20	0.23790817034409173
1104	21	0.37554955099302845
1104	22	0.40423928217875016
1104	23	0.8832926586472638
1104	24	0.5144872292328557
1104	25	0.5570985857326392
1105	1	-0.2651118278368081
1105	2	-0.10437171048129201
1105	3	-1.453493257525988
1105	4	-0.5491041969509496
1105	5	-0.4667131806878473
1105	6	-0.16617955183988897
1105	7	-0.19662450377518326
1105	8	-0.15731705232665405
1105	9	0.2536708591250232
1105	10	0.33971355907830514
1105	11	-0.20544619571114708
1105	12	-0.23675890259111299
1105	13	-0.2357973117019867
1105	14	0.5632377538407027
1105	15	0.18464875250373325
1105	16	-0.17624753937929682
1105	17	0.32759685523857485
1105	18	0.2704724233198945
1105	19	0.15118108759029525
1105	20	-0.42526783604344587
1105	21	-0.048714165838710244
1105	22	0.07391285099485856
1105	23	0.29697742890581225
1105	24	0.19052661258115222
1105	25	0.4260124648298742
1106	1	-1.6642041425816316
1106	2	-1.1161465902710326
1106	3	-1.1191236777441347
1106	4	-1.280675308347874
1106	5	-0.8188752177667595
1106	6	-0.6861412589081013
1106	7	-0.5926373847288673
1106	8	-0.6900018667708778
1106	9	-0.31129102142594434
1106	10	-1.9357469252047963
1106	11	-1.2434842177134584
1106	12	-0.9135707842841541
1106	13	-0.9427890089044269
1106	14	-0.23558743323834364
1106	15	-0.9442671734631247
1106	16	-1.4361388027420077
1106	17	-0.7724674841225081
1106	18	-0.6559170572584421
1106	19	-0.6002534665659383
1106	20	-0.3086667070207696
1106	21	-0.35537213741707774
1106	22	-0.311506391405203
1106	23	-0.6283506898273146
1106	24	-0.8680529934407986
1106	25	-1.6151907614485097
1107	1	-0.11467381919662145
1107	2	0.08318866121684465
1107	3	-0.08552131101521354
1107	4	0.12166185158619669
1107	5	-0.10651051198599504
1107	6	-0.03279195510141619
1107	7	-0.10041396514395018
1107	8	-0.28402171092314177
1107	9	0.36179713079624065
1107	10	-0.08595048092804092
1107	11	-0.07960004111269114
1107	12	-0.0895623906139349
1107	13	0.11863050156368615
1107	14	-1.2361004632292776
1107	15	-0.5809090233528735
1107	16	-0.37519856258874285
1107	17	-0.06469926556938788
1107	18	0.286385558526149
1107	19	0.08102726138634608
1107	20	0.18381913062435729
1107	21	0.3435681453748529
1107	22	-0.28652027946270664
1107	23	0.5678318860135292
1107	24	0.44160129332823317
1107	25	0.03002904648528215
1108	1	-0.2659245174070435
1108	2	0.13249725010765445
1108	3	-0.16245234769442263
1108	4	-0.4669931718648286
1108	5	-0.4204460361310396
1108	6	-0.366295629334374
1108	7	-0.30111146920020104
1108	8	-0.3347651785196888
1108	9	0.25207637283197215
1108	10	0.23418774594092184
1108	11	-0.036409101535639365
1108	12	-0.07143998326618117
1108	13	-0.11880726672736586
1108	14	0.04506838955925474
1108	15	-0.02983682577185931
1108	16	-0.18840132207000693
1108	17	0.1360682970510254
1108	18	0.09801703990375188
1108	19	0.17814206300123203
1108	20	0.2253627025486864
1108	21	0.25103891637182585
1108	22	0.19287060593462257
1108	23	0.028023131022784753
1108	24	0.11617579288660806
1108	25	-0.7229728349341542
1109	1	-0.8749862979437104
1109	2	-0.7075465008866253
1109	3	-0.7571626109741203
1109	4	-0.6413393088033317
1109	5	-0.9881972142181894
1109	6	-1.1618043775325688
1109	7	-0.8540932224065045
1109	8	-1.164591989462906
1109	9	-0.9215837040045685
1109	10	-1.1187380854802313
1109	11	-1.254023882610717
1109	12	-1.1114715912683266
1109	13	-1.2295097832582371
1109	14	-0.6195204551712976
1109	15	-1.1816884593833734
1109	16	-1.3559548595872855
1109	17	-1.4034251316864328
1109	18	-1.9228923038359724
1109	19	-2.096051058758185
1109	20	-1.74368098606692
1109	21	-1.5634046862951507
1109	22	-1.7816554983184645
1109	23	-1.1550793963074781
1109	24	-2.1924611177070616
1109	25	-1.1047335342559421
1110	1	0.021407757770269598
1110	2	0.21231744473150727
1110	3	-0.02987829575106815
1110	4	0.14610958167051535
1110	5	0.11440176860619289
1110	6	0.11465834763234994
1110	7	0.03667293051640286
1110	8	0.0010245772086676652
1110	9	0.3184037432268383
1110	10	0.30155975222766074
1110	11	0.1812058508182801
1110	12	0.19054880871930635
1110	13	0.20407219505172236
1110	14	-2.489649135084413
1110	15	-0.6394142816458555
1110	16	-0.07645993821742536
1110	17	-0.2331484722193906
1110	18	-2.893394407876393
1110	19	-0.5012445164371897
1110	20	-1.921251444251773
1110	21	-0.32396523390119114
1110	22	-1.8150836652028346
1110	23	-0.5314169457097577
1110	24	-0.014477903777674328
1110	25	-0.047672880528260464
1111	1	-0.1539180461827243
1111	2	-1.3354149583403263
1111	3	-0.31522866252647574
1111	4	0.06468546478296844
1111	5	-0.02643598962326621
1111	6	0.07910582644604602
1111	7	-0.057234791302462895
1111	8	-0.016881263496962617
1111	9	-1.5963098387268033
1111	10	-0.3135687180405853
1111	11	-0.13548393399931768
1111	12	-0.08542938942676415
1111	13	0.0912351226218675
1111	14	0.9104130877444732
1111	15	0.32009108794799346
1111	16	0.28749108679036084
1111	17	-0.07657158564735767
1111	18	0.18508555528762827
1111	19	-0.04841207052057683
1111	20	0.32334279268845734
1111	21	-0.5607616651957059
1111	22	-0.2001364537660706
1111	23	0.022356402790528307
1111	24	-0.4754107775838605
1111	25	-1.9484098949379955
1112	1	0.20718056613132774
1112	2	0.367070616435754
1112	3	-0.020031159415538013
1112	4	0.18720316584182228
1112	5	0.06483007904747495
1112	6	0.09193434009971213
1112	7	0.07864450226533393
1112	8	0.10000522570152981
1112	9	-0.3012185975082744
1112	10	0.5519457394256122
1112	11	0.15852762348055194
1112	12	0.10246599500290898
1112	13	0.07195164626749317
1112	14	0.48270777153375377
1112	15	0.15858480743763437
1112	16	0.23497925127000485
1112	17	-3.7235744350585307
1112	18	-1.0181549114429131
1112	19	-1.2116830258621718
1112	20	-0.1367150527076717
1112	21	-0.47071987900898193
1112	22	-1.7363657333114233
1112	23	-1.5119638784171698
1112	24	-0.3634609740897261
1112	25	0.08995342027183284
1113	1	-0.20337411698954355
1113	2	-0.11038863865579529
1113	3	-0.15811898809221958
1113	4	-0.42761255165720513
1113	5	-0.22846780760553997
1113	6	-0.26552261410740385
1113	7	-0.32791386342298523
1113	8	-0.22351731312276968
1113	9	0.16176496394007955
1113	10	0.3390861578120621
1113	11	-0.10891525557503456
1113	12	-0.11601483164839976
1113	13	-0.3899877592304506
1113	14	-1.5744167163970246
1113	15	-1.672491287208615
1113	16	-0.5259632355500798
1113	17	-0.25627208664873535
1113	18	-0.552727956697062
1113	19	-0.09590004382184247
1113	20	0.32633634449864246
1113	21	-1.4501755162574597
1113	22	-2.010554755576699
1113	23	-0.614774136021154
1113	24	-2.2405548533434185
1113	25	-0.4998726949008727
1114	1	0.23933836362900607
1114	2	0.3933416687813356
1114	3	0.030514460170572022
1114	4	0.4090517685838009
1114	5	0.11558055585575744
1114	6	0.22749469330104688
1114	7	0.24041818965591233
1114	8	0.1279522443757486
1114	9	0.5161442627445254
1114	10	0.39457558134543547
1114	11	0.306533250015363
1114	12	0.32571323537115404
1114	13	0.28230650875469365
1114	14	1.1062461929618834
1114	15	0.5479064417230077
1114	16	0.4846807780137823
1114	17	-0.2925962840554419
1114	18	-0.022482928511720957
1114	19	-0.42356722240572253
1114	20	-1.5283942906703345
1114	21	-0.5673776880502928
1114	22	-1.2470971997298719
1114	23	-2.74631648623609
1114	24	-1.96795336120568
1114	25	-1.342420778079287
1115	1	0.44112680609023514
1115	2	0.5516862501691477
1115	3	0.41032519353077035
1115	4	0.6193717875160729
1115	5	0.49432221137509913
1115	6	0.3921811051691506
1115	7	0.40607492385695565
1115	8	0.5237907987994603
1115	9	0.7157159408592761
1115	10	1.0001079836211915
1115	11	0.7025616164826971
1115	12	0.5501905129339946
1115	13	0.557940830312269
1115	14	1.2135069425438942
1115	15	0.7262595006146029
1115	16	0.6882475031710555
1115	17	0.7019314735814888
1115	18	0.8557396579846142
1115	19	0.2440187878854232
1115	20	0.7739075077448092
1115	21	-0.17741546410071862
1115	22	0.8686413774237991
1115	23	0.9519910494243575
1115	24	1.0151094395983833
1115	25	0.9685317150170079
1116	1	0.5570716060068754
1116	2	0.6278018954686644
1116	3	0.4166876030758466
1116	4	0.7212456569985122
1116	5	0.580594262439071
1116	6	0.4455782732733482
1116	7	0.4987177129350542
1116	8	0.493542484232826
1116	9	0.7384589708140012
1116	10	0.6534821853410406
1116	11	0.5046223176821059
1116	12	0.5596892941292495
1116	13	0.017985018068408465
1116	14	1.0618253309022094
1116	15	0.734303864314164
1116	16	0.7809845012951655
1116	17	0.9953020482710855
1116	18	0.99640699549564
1116	19	0.99974936948297
1116	20	0.8573408127517368
1116	21	0.3192486415316722
1116	22	0.3344258993786866
1116	23	-0.53036106887307
1116	24	-0.060663656850699965
1116	25	-0.6222669491109798
1201	1	0.5331561052237674
1201	2	0.6872113860242486
1201	3	0.5734697644587179
1201	4	0.5472265797747866
1201	5	0.5216673702431862
1201	6	0.4332833705912975
1201	7	0.3902194156598924
1201	8	0.4298642074254815
1201	9	0.6854094728504023
1201	10	0.9628640555124619
1201	11	0.5991715625918914
1201	12	0.6495044371246124
1201	13	0.5838348078820772
1201	14	1.1418797414624535
1201	15	0.7694817363950648
1201	16	0.7048462780362499
1201	17	0.7226524152390255
1201	18	0.7515495358365669
1201	19	0.9497838949631756
1201	20	1.0252858002906322
1201	21	0.932388839727885
1201	22	1.0561267003698305
1201	23	0.6579245354785002
1201	24	0.9267685893890203
1201	25	1.1765363538399285
1202	1	0.33439540043625016
1202	2	0.691156975941638
1202	3	0.5008993641770729
1202	4	0.42157443075820566
1202	5	0.22594306420750598
1202	6	0.1653017311487588
1202	7	0.31991616106515863
1202	8	0.2958254927493371
1202	9	0.7949167804540735
1202	10	-0.07564348820802991
1202	11	0.2794409266458615
1202	12	0.26545282085545097
1202	13	0.2990957664598284
1202	14	1.0459390774793178
1202	15	0.7448923605211135
1202	16	0.5780761087814881
1202	17	-1.3551698170688269
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2210	16	0.22118661566448594
2210	17	0.620204168022461
2210	18	0.5908148844145131
2210	19	0.7063554185745744
2210	20	0.8265615610252439
2210	21	0.9211486033247596
2210	22	1.1791112495861995
2210	23	1.4740584000299464
2210	24	1.147322918811632
2210	25	1.1132114483896132
2211	1	0.051387390847324355
2211	2	-0.6548866009802907
2211	3	-0.09031131664134406
2211	4	0.19862225071921102
2211	5	0.16451493393709882
2211	6	0.12556940182548004
2211	7	0.20794568827561477
2211	8	0.2755954086043033
2211	9	0.7347180125382664
2211	10	0.7344224222140099
2211	11	0.386268096477977
2211	12	0.19443702513693495
2211	13	0.1357050332007148
2211	14	-1.0255601380608184
2211	15	-0.05252734785270263
2211	16	0.18425881685993062
2211	17	0.2749060280526834
2211	18	0.26955921671570593
2211	19	0.5960733344206375
2211	20	0.6850621521506426
2211	21	0.6516721897996689
2211	22	0.11134173180166584
2211	23	0.5886246643361588
2211	24	-0.01312180300647725
2211	25	0.38274765766342256
2212	1	-0.040001753895687386
2212	2	-1.0010149650703113
2212	3	-0.2841269258395691
2212	4	0.15279497931245345
2212	5	0.1572885424872439
2212	6	0.1646263321299628
2212	7	0.06368599621507798
2212	8	0.12699115615139078
2212	9	-2.2553619114603407
2212	10	0.07258282256864135
2212	11	0.06952531846975774
2212	12	0.08327111080377136
2212	13	0.06966061749429286
2212	14	0.6942312077360313
2212	15	0.31882855676778377
2212	16	0.34367407321449406
2212	17	0.4307311165248606
2212	18	0.19807613158386062
2212	19	-0.39597497964408745
2212	20	0.23829850320552737
2212	21	0.31072927627779734
2212	22	-1.6676270124523
2212	23	-0.2150129694559908
2212	24	0.3299289690837739
2212	25	-0.2624082263592342
2213	1	0.23964010660158763
2213	2	0.41608297680508244
2213	3	0.15109730798642573
2213	4	0.33004239289862625
2213	5	0.3439864345483059
2213	6	0.229747160033277
2213	7	0.20279436879741297
2213	8	0.23279006728657947
2213	9	0.6449602305377837
2213	10	0.6668435609217767
2213	11	0.11036062062915875
2213	12	0.1502539684997828
2213	13	0.14724585340901797
2213	14	-1.5876044443403359
2213	15	-0.38555091040250034
2213	16	-0.05794352856473495
2213	17	-2.2388118328258955
2213	18	-0.9107684664799649
2213	19	-0.44998597227361614
2213	20	0.3186186343429611
2213	21	-0.09458916686262597
2213	22	0.35734589741538514
2213	23	0.8923883580974561
2213	24	0.6814905354074696
2213	25	-0.9427692812699036
2214	1	0.2646988904566463
2214	2	0.2688573356054569
2214	3	0.18918238648138797
2214	4	0.5029888049004541
2214	5	0.40254509131566607
2214	6	0.3445536094513528
2214	7	0.31101012978577103
2214	8	0.26628988458062963
2214	9	0.6625667807361282
2214	10	0.6575698295879562
2214	11	0.05893259425943853
2214	12	0.06053869129750211
2214	13	0.1291680497811143
2214	14	0.9262986163875624
2214	15	0.5389018384646036
2214	16	0.5354361013996325
2214	17	0.4779354524936892
2214	18	0.4506320047549295
2214	19	-0.7637433873450163
2214	20	0.16589748366811818
2214	21	0.41816419048667086
2214	22	0.7325558424033716
2214	23	1.182630321584817
2214	24	0.874467934190136
2214	25	0.9510874235008302
2215	1	-0.23086334080654325
2215	2	0.17269612003781631
2215	3	0.07467018773131784
2215	4	0.2626736225562496
2215	5	0.18642159429690963
2215	6	0.139203902586857
2215	7	0.1442612702396886
2215	8	0.09948597724053343
2215	9	0.21611505362314176
2215	10	0.6211960434250727
2215	11	0.30536000351511916
2215	12	0.1670000531603364
2215	13	0.05980454013037928
2215	14	0.8518903524552854
2215	15	0.37319515432456285
2215	16	0.46080722128316154
2215	17	0.5060750569515378
2215	18	0.3602469270495803
2215	19	-0.3870902858296006
2215	20	-1.6606579491278382
2215	21	-1.9359115729434535
2215	22	-0.241531059935852
2215	23	0.4234553104375962
2215	24	0.5799149124838578
2215	25	-0.17339323040561538
2216	1	0.04392870405295923
2216	2	-0.11071824006916486
2216	3	0.024159326514478607
2216	4	0.30688068966108517
2216	5	0.3296508593307479
2216	6	0.12932248756609255
2216	7	0.19511771731195182
2216	8	0.11020276792852712
2216	9	0.4232977734415968
2216	10	-1.4909374567019498
2216	11	-0.30092849623788
2216	12	-0.026377044999819077
2216	13	0.1431299115092699
2216	14	-0.4230991859068922
2216	15	-0.309211037187481
2216	16	0.1194997128268227
2216	17	0.39876198327420553
2216	18	0.263808068564245
2216	19	-1.1902678463015732
2216	20	-0.9371275222411697
2216	21	-0.797325016129534
2216	22	-0.5569269986725018
2216	23	-4.3822055651107625
2216	24	-1.880040715358831
2216	25	-0.9817412840965929
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	18.0
4	33.0
5	63.0
6	90.0
7	381.0
8	185.0
9	465.0
10	1411.0
11	3030.0
12	6167.0
13	10523.0
14	15248.0
15	19908.0
16	23618.0
17	26355.0
18	27777.0
19	28634.0
20	29053.0
21	29484.0
22	30625.0
23	32208.0
24	34599.0
25	38137.0
26	43052.0
27	49634.0
28	58034.0
29	71118.0
30	88169.0
31	109776.0
32	145368.0
33	195260.0
34	265073.0
35	367651.0
36	525967.0
37	786683.0
38	1351745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98744311357838	21.102808402656255	13.791269924554875	23.118478559210494
2	15.787168026231951	21.340459716012322	40.34845311272232	22.523919145033407
3	18.02446700158033	26.587169979557217	30.215559893001608	25.172803125860842
4	12.562576248608126	17.74984906032263	38.47605864502046	31.211516046048775
5	13.233937029371415	37.833514022990066	35.36308425349116	13.569464694147355
6	30.25180314025693	38.80503705945323	17.8091726960101	13.133987104279743
7	25.58480783963025	33.052340160074365	23.928092023508743	17.434759976786633
8	23.267555868101912	37.07987203220355	21.693672499497612	17.958899600196922
9	24.242325381921294	17.499699247813684	22.235588986058467	36.022386384206555
10	14.806691958432586	30.218306841008904	33.79984469756345	21.175156502995055
11	31.58471888732841	26.03923345263444	23.93172654374122	18.444321116295928
12	20.85966806627802	27.698646166516827	31.518459399152444	19.92322636805271
13	26.08647687687252	24.512821163357646	27.139643057942266	22.261058901827568
14	20.868116018862512	23.71344278013174	28.19410517024428	27.224336030761464
15	22.587121664369793	31.002491860503838	24.45219961798571	21.958186857140657
16	21.82157724567691	31.358821953053273	25.84645620686226	20.973144594407557
17	20.068632199098165	30.411371860209407	28.52744087387469	20.992555066817744
18	20.113259770512816	30.140564355159405	29.422168114959707	20.32400775936807
19	21.01132024869392	28.987841650614392	29.537681007098033	20.463157093593647
20	22.176006777157898	28.54886824634244	28.873501357923182	20.40162361857648
21	22.68307576895583	28.93585609615334	27.796635430360674	20.584432704530155
22	21.548902012946066	28.481972643649833	29.31379886486369	20.655326478540413
23	21.81516622246143	28.777137632950105	28.618596456415563	20.789099688172904
24	21.462206025725127	28.050169978653	28.90198864151176	21.585635354110114
25	22.040580903568323	28.435335776210557	28.346233182168778	21.17785013805234
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2965.0
1	2965.0
2	3570.0
3	4175.0
4	4175.0
5	4175.0
6	6207.5
7	8240.0
8	8240.0
9	8240.0
10	12898.5
11	17557.0
12	17557.0
13	17557.0
14	28641.5
15	39726.0
16	39726.0
17	39726.0
18	63480.5
19	87235.0
20	87235.0
21	87235.0
22	125115.0
23	162995.0
24	162995.0
25	162995.0
26	215020.5
27	267046.0
28	267046.0
29	267046.0
30	322973.0
31	378900.0
32	378900.0
33	378900.0
34	426989.0
35	475078.0
36	475078.0
37	475078.0
38	507539.5
39	540001.0
40	540001.0
41	540001.0
42	543186.5
43	546372.0
44	546372.0
45	546372.0
46	523309.5
47	500247.0
48	500247.0
49	500247.0
50	473130.0
51	446013.0
52	446013.0
53	446013.0
54	401201.5
55	356390.0
56	356390.0
57	356390.0
58	306894.0
59	257398.0
60	257398.0
61	257398.0
62	211619.0
63	165840.0
64	165840.0
65	165840.0
66	127663.5
67	89487.0
68	89487.0
69	89487.0
70	65702.5
71	41918.0
72	41918.0
73	41918.0
74	29850.0
75	17782.0
76	17782.0
77	17782.0
78	12331.0
79	6880.0
80	6880.0
81	6880.0
82	4620.5
83	2361.0
84	2361.0
85	2361.0
86	1535.5
87	710.0
88	710.0
89	710.0
90	442.0
91	174.0
92	174.0
93	174.0
94	108.0
95	42.0
96	42.0
97	42.0
98	26.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07838222351865297
2	0.017506344634475226
3	0.028762040990664338
4	0.03458239101790901
5	0.04296188327503169
6	0.016849573619727772
7	0.019114301256787956
8	0.03666594044400438
9	0.03643946768029836
10	0.021061967024659713
11	0.021333734341106934
12	0.016872220896098373
13	0.023643756530908323
14	0.038681548040987945
15	0.03557887117821549
16	0.023756992912761333
17	0.022556687265119436
18	0.02864880460881133
19	0.01807252654374027
20	0.01612486077586851
21	0.013452482164137494
22	0.02079019970821249
23	0.020744905155471286
24	0.019703130442423604
25	0.11088106511046662
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4415542.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.8721725649229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.4185179422949	67.29597742007275
2	9.936549376684509	15.475613755221065
3	1.9676679157779697	4.596797264637727
4	0.6285380813350558	1.9578250373339607
5	0.3253223011759322	1.2666777188195009
6	0.1780001509099789	0.8316755080946517
7	0.11241885727803567	0.6128010457454643
8	0.08262416977336494	0.5147298885299969
9	0.0542955183560277	0.3805298977430276
>10	0.2725481369873271	3.6769580735798875
>50	0.0143833799921599	0.778987923221537
>100	0.0079665856315458	1.1438956477929931
>500	6.713608703920195E-4	0.3812826532723573
>1k	4.0865417999334314E-4	0.6609593238696416
>5k	8.756875285543464E-5	0.42528884206552275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6851	0.15515649041499324	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6613	0.14976643863879	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5246	0.11880761184017727	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.264727637060184E-5	0.0
3	0.0	0.0	0.0	2.264727637060184E-5	0.0
4	0.0	0.0	0.0	6.794182911180553E-5	0.0
5	0.0	0.0	0.0	6.794182911180553E-5	0.0
6	0.0	0.0	0.0	6.794182911180553E-5	0.0
7	0.0	0.0	0.0	9.058910548240736E-5	0.0
8	0.0	0.0	0.0	9.058910548240736E-5	0.0
9	9.058910548240736E-5	0.0	0.0	1.3588365822361106E-4	0.0
10	9.058910548240736E-5	0.0	0.0	1.3588365822361106E-4	0.0
11	9.058910548240736E-5	0.0	0.0	1.585309345942129E-4	0.0
12	9.058910548240736E-5	0.0	0.0	1.8117821096481473E-4	0.0
13	9.058910548240736E-5	2.264727637060184E-5	0.0	2.0382548733541658E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCG	105	1.9905201E-8	12.669635	5
CGCAGAT	175	2.3646862E-10	10.312613	8
GGTATCA	4900	0.0	9.625235	1
TGGACCG	90	0.0011119819	9.502228	5
TAGGACC	265	0.0	9.322939	4
GCGTCAG	160	6.6596294E-7	8.9145	1
TAGACTG	565	0.0	8.745412	5
CAATACG	100	0.0029004333	8.552005	4
GTCTTAG	415	0.0	8.477726	1
CGTCAGA	180	4.026715E-7	8.446136	2
CTTAGAC	365	0.0	8.330625	3
GGAGCGT	115	0.0011130465	8.25953	6
GTAGGAC	315	0.0	8.144673	3
GTGTAGA	680	0.0	8.110447	1
TAGGACA	495	0.0	8.062495	4
GTCCTGG	815	0.0	8.050395	1
CGCAAAA	130	4.1794722E-4	8.040072	2
TTAGACT	415	0.0	8.0139265	4
CTTACAC	570	0.0	8.001784	3
GTCTAGA	500	0.0	7.987392	1
>>END_MODULE