FastQCFastQC Report
Wed 25 May 2016
SRR1295318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415542
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT189570.4293244181574991No Hit
GGTATCAACGCAGAGTACTTTTTTT157320.3562869518623082No Hit
TATCAACGCAGAGTACTTTTTTTTT109490.24796502898171957No Hit
GTGGTATCAACGCAGAGTACATGGG78600.17800759227293048No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70510.1596859456891136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3000.011.4080943
GACTGCG909.559821E-510.5515677
GGTATCA60600.010.25569251
GTACTAG2750.09.690581
GCAGCGT3450.09.3795991
ACTGCGT1151.1161051E-49.0835238
CAGCGTC3800.09.006392
TAGACTG6550.08.9987525
TATACCG850.00739618.9475255
TACCCGC850.0074616858.93789911
GACCGTG1701.6970989E-78.9377987
TAGGACT4900.08.9246994
TTAGACT4050.08.6851744
GTATTAG4400.08.6523051
TCGCGTT1107.17501E-48.63319910
CTAGGAC4300.08.6223973
GTTCTAG5100.08.5844421
TCTAGAC4100.08.5792583
AACCGTG1000.0029165558.5467697
CTGTGCG1451.6985463E-58.5141199
Produced by FastQC (version 0.11.5)