FastQCFastQC Report
Wed 25 May 2016
SRR1295317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587074
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1954547.555021619018243No Hit
GCTTATGTACTCTGCGTTGATACCA1195214.6199296966379775No Hit
CTTATGTACTCTGCGTTGATACCAC734922.840738223954939No Hit
GAGTACATAAGCAGTGGTATCAACG481921.8627994406035544No Hit
TATGTACTCTGCGTTGATACCACTG455811.7618746120134174No Hit
GCGTTGATACCACTGCTTATGTACT398801.5415098292511153No Hit
CCCATGTACTCTGCGTTGATACCAC368501.4243890974900602No Hit
CATAAGCAGTGGTATCAACGCAGAG316711.2242015497044152No Hit
CATGTACTCTGCGTTGATACCACTG294251.1373853241151972No Hit
ACTCTGCGTTGATACCACTGCTTAT286521.1075060087187303No Hit
GTACATGATAAGCAGTGGTATCAAC273851.0585317621374573No Hit
CTGCTTATGTACTCTGCGTTGATAC269561.0419493218980207No Hit
ATCATGTACTCTGCGTTGATACCAC268941.039552792073207No Hit
GTATCAACGCAGAGTACATAAGCAG262081.0130363491728493No Hit
ACGCAGAGTACATAAGCAGTGGTAT260371.0064265653011857No Hit
TATCAACGCAGAGTACATAAGCAGT211310.8167914794860911No Hit
GGTATCAACGCAGAGTACATAAGCA185970.7188429863235454No Hit
AAGCAGTGGTATCAACGCAGAGTAC166350.6430044134802484No Hit
GTGGTATCAACGCAGAGTACATAAG145350.5618316290913982No Hit
GTACATGGGAAGCAGTGGTATCAAC134320.519196590433826No Hit
GCAGTGGTATCAACGCAGAGTACAT127810.49403302727328247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA120720.46662754911533266No Hit
GTACTCTGCGTTGATACCACTGCTT117400.45379451844052393No Hit
ACATAAGCAGTGGTATCAACGCAGA111760.4319938277760899No Hit
CCACTGCTTATGTACTCTGCGTTGA105500.4077966072868422No Hit
GAGTACATGATAAGCAGTGGTATCA105210.406675649788139No Hit
GTACATGGGATAAGCAGTGGTATCA95310.36840848000482396No Hit
GATACCACTGCTTATGTACTCTGCG94900.3668236780238988No Hit
ATACCACTGCTTATGTACTCTGCGT94730.3661665650074176No Hit
GTTGATACCACTGCTTATGTACTCT92010.35565275674371893No Hit
GCTTATCATGTACTCTGCGTTGATA86880.33582340512872844No Hit
GCTTATCCCATGTACTCTGCGTTGA83760.32376344859095646No Hit
GCGTTGATACCACTGCTTATCATGT80400.3107758030887404No Hit
CAGTGGTATCAACGCAGAGTACATA77970.3013829523237449No Hit
GATAAGCAGTGGTATCAACGCAGAG72350.27965956907301454No Hit
TACCACTGCTTATGTACTCTGCGTT71680.2770697707139417No Hit
CCCCATGTACTCTGCGTTGATACCA69790.2697642201189452No Hit
TGATACCACTGCTTATGTACTCTGC69380.26817941813802004No Hit
GTACTTTTTTTTTTTTTTTTTTTTT62750.2425520104952545No Hit
GCAGAGTACATAAGCAGTGGTATCA61950.2394597139471078No Hit
GTGGTATCAACGCAGAGTACATGGG61820.23895721575803397No Hit
ACGCAGAGTACATGATAAGCAGTGG61190.23652203222636847No Hit
GTATCAACGCAGAGTACATGATAAG58040.22434611456804096No Hit
TATCAACGCAGAGTACATGATAAGC57990.22415284603378177No Hit
GAGTACATGGGAAGCAGTGGTATCA56690.21912786414304344No Hit
GGATAAGCAGTGGTATCAACGCAGA55370.21402557483860143No Hit
GAACAAAAAAAAAAAAAAAAAAAAA53890.20830482622453012No Hit
GTATCAACGCAGAGTACTTTTTTTT51180.19782967166768325No Hit
GGGATAAGCAGTGGTATCAACGCAG50630.19570371779083243No Hit
CAGTGGTATCAACGCAGAGTACATG49510.1913745026234271No Hit
GTACATGGTAAGCAGTGGTATCAAC49050.18959643210824276No Hit
ATCAACGCAGAGTACATAAGCAGTG48840.18878470426435426No Hit
GCGTTGATACCACTGCTTCCCATGT48460.18731586340398457No Hit
ATGATAAGCAGTGGTATCAACGCAG48180.18623355961213325No Hit
GTACACAAGCAGTGGTATCAACGCA47860.18499664099287458No Hit
GTATCAACGCAGAGTACATGGGAAG47500.18360510754620857No Hit
CTGCTTATCATGTACTCTGCGTTGA46050.17800032005269273No Hit
GAGTACATGGGATAAGCAGTGGTAT45740.1768020551402859No Hit
CATGTAAGCAGTGGTATCAACGCAG45630.17637686436491573No Hit
GCTTACATGTACTCTGCGTTGATAC45590.1762222495375084No Hit
GGTATCAACGCAGAGTACATGATAA45510.17591301988269373No Hit
GAGTACATGTAAGCAGTGGTATCAA45230.1748307160908424No Hit
ACCATGTACTCTGCGTTGATACCAC43860.16953515825214122No Hit
GTACATAAGCAGTTGTATCAACGCA42900.16582440239436522No Hit
AAAAAGTACTCTGCGTTGATACCAC39680.1533779087880749No Hit
TACATGTACTCTGCGTTGATACCAC39280.15183176051400152No Hit
GCGTTGATACCACTGCTTATCCCAT38320.14812100465622552No Hit
CCATGTACTCTGCGTTGATACCACT38080.1471933156917815No Hit
ATGTACTCTGCGTTGATACCACTGC37730.14584043595196736No Hit
TATCAACGCAGAGTACATGGGAAGC37630.14545389888344903No Hit
GTACATGTAAGCAGTGGTATCAACG36890.14259352457641336No Hit
CATGATAAGCAGTGGTATCAACGCA36760.14209102638733953No Hit
ACGCAGAGTACATGGGAAGCAGTGG35380.13675681484178653No Hit
GGTATCAACGCAGAGTACATGGGAA34460.13320067381141784No Hit
GTATCAACGCAGAGTACATGGGATA34020.13149991070993716No Hit
ACTCTGCGTTGATACCACTGCTTCC33970.131306642175678No Hit
TACATAAGCAGTGGTATCAACGCAG33430.129219342005679No Hit
TACCACTGCTTATCATGTACTCTGC33250.128523575282346No Hit
GGTATCAACGCAGAGTACTTTTTTT32410.12527666390679199No Hit
TATCAACGCAGAGTACTTTTTTTTT32050.123885130460126No Hit
CTGCGTTGATACCACTGCTTATGTA31030.11994245236123899No Hit
GTGGTATCAACGCAGAGTACATGAT30750.11886014856938765No Hit
GCTTATGTACTCTTCGTTGATACCA29930.11569054460753732No Hit
GTACTGGTTCACTATCGGTCAGTCA28890.11167055909494664No Hit
ACGCAGAGTACATGGGATAAGCAGT28850.11151594426753932No Hit
AAAGTACTCTGCGTTGATACCACTG27440.1060657716014308No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA27080.10467423815476481No Hit
GCTTCCCATGTACTCTGCGTTGATA26530.10254828427791397No Hit
GGTATCAACGCAGAGTACATGGGAT26230.10138867307235896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTTC250.00604009218.996216
AGTTGTA6250.016.26075611
TTACCAC1650.015.55889719
CAGTTGT6650.015.42549510
GCAGTTG6750.015.1969699
TTGTATC6500.015.19696713
GTTGTAT6700.014.8850912
CGCAAAA2950.014.816622
TATAGGT456.75389E-414.7808314
ATACCGT456.774716E-414.774836
TAACGCA1500.014.57926419
AGCAGTT7200.014.3793548
GCGCTAC602.5743986E-514.24715813
GCAGACT400.00528735114.2471579
CTTCGTT4150.014.18994112
TTCGTTG4250.014.07954513
TGTATCA7100.013.91271714
GTACAAA7000.013.8537551
CTCTTCG4300.013.69494210
TCGTTGA4300.013.2531714
Produced by FastQC (version 0.11.5)