##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295317_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2587074
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.802707614857557	31.0	30.0	33.0	25.0	34.0
2	29.64588450117778	31.0	30.0	34.0	23.0	34.0
3	30.11055539965227	31.0	30.0	34.0	25.0	34.0
4	33.26353942716752	35.0	33.0	37.0	26.0	37.0
5	33.816362036802964	35.0	35.0	37.0	28.0	37.0
6	33.8491983607736	37.0	35.0	37.0	28.0	37.0
7	33.930338289511624	37.0	35.0	37.0	28.0	37.0
8	33.78362582593308	36.0	35.0	37.0	28.0	37.0
9	34.80554866231117	38.0	35.0	39.0	26.0	39.0
10	34.660278368535266	38.0	34.0	39.0	26.0	39.0
11	35.17750439299378	39.0	35.0	39.0	27.0	39.0
12	35.18855355509738	39.0	35.0	39.0	27.0	39.0
13	34.83197465553749	38.0	35.0	39.0	27.0	39.0
14	35.07353365230372	39.0	33.0	40.0	23.0	41.0
15	35.59670886878381	39.0	34.0	41.0	26.0	41.0
16	35.445143045772944	38.0	34.0	40.0	26.0	41.0
17	35.589983896865725	39.0	34.0	40.0	27.0	41.0
18	35.552261744349025	39.0	34.0	40.0	26.0	41.0
19	35.631169421516354	39.0	34.0	41.0	26.0	41.0
20	35.68237050814936	39.0	34.0	41.0	26.0	41.0
21	35.692608328946136	39.0	34.0	41.0	26.0	41.0
22	35.45226537779747	39.0	34.0	41.0	25.0	41.0
23	35.09654613667796	39.0	33.0	40.0	23.0	41.0
24	35.412293966079055	39.0	34.0	41.0	25.0	41.0
25	34.20380708089525	38.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4043198652347577
1101	2	0.6055614381867258
1101	3	0.32021017146342956
1101	4	0.26559011154232337
1101	5	0.17650095568316715
1101	6	0.21623112099433683
1101	7	0.2359414275562841
1101	8	0.21017261898184358
1101	9	0.1983539466077815
1101	10	0.4377122799434332
1101	11	0.4035128833936028
1101	12	0.33545642615693794
1101	13	0.4959094200739571
1101	14	1.1356075107094696
1101	15	0.6668885507892313
1101	16	0.5438638985481035
1101	17	0.8480609889334261
1101	18	0.7810143996323617
1101	19	0.7964843300920919
1101	20	0.8909171477514732
1101	21	0.7860332443494684
1101	22	0.5971482184768462
1101	23	-1.6966489208244226
1101	24	-0.061285728686129914
1101	25	0.42826200988664453
1102	1	0.431772593057989
1102	2	0.41797636049767206
1102	3	0.3310944005433427
1102	4	0.4471284704071863
1102	5	0.26334655227921644
1102	6	0.27037751051091163
1102	7	0.22115321911274322
1102	8	0.26698010310840203
1102	9	0.4144014231461455
1102	10	0.8886906007763287
1102	11	0.5679599023045583
1102	12	0.474683731004518
1102	13	0.42020873572840145
1102	14	1.0289059928783288
1102	15	0.6281960889021647
1102	16	0.5310426128392862
1102	17	0.750754696593539
1102	18	0.7038155552171403
1102	19	0.8136472012130227
1102	20	0.9644300476661556
1102	21	0.8749737086027167
1102	22	0.8552048309709477
1102	23	0.9243281961463055
1102	24	0.9725088762046497
1102	25	0.7487344818483024
1103	1	-0.2041091111377149
1103	2	-1.247728692598379
1103	3	-0.27883841514925933
1103	4	0.12486813906110683
1103	5	0.02202676074593768
1103	6	0.08605151224897867
1103	7	0.12903419042553566
1103	8	0.31883024019496276
1103	9	0.8076297343926058
1103	10	0.9099793181755658
1103	11	0.5294359097528343
1103	12	0.4157662466352221
1103	13	0.27370567032416915
1103	14	-0.9901060045363366
1103	15	-0.08402564461898265
1103	16	0.08930534883086949
1103	17	0.44724709521097594
1103	18	0.6952826951871458
1103	19	0.768359478874423
1103	20	0.7264909189566353
1103	21	0.7661208576224112
1103	22	0.16110119788613986
1103	23	1.1827760255627453
1103	24	0.949427366769541
1103	25	0.6626326344063784
1104	1	0.08314286334484322
1104	2	0.1129624671891456
1104	3	-0.2523592196010611
1104	4	-0.12516268547143028
1104	5	-0.015909676433402353
1104	6	0.07555101668533837
1104	7	0.061534787156872994
1104	8	0.0552744933692324
1104	9	-2.1532628255136856
1104	10	-0.5194709012582237
1104	11	-0.37322963511161333
1104	12	-0.07270788104368364
1104	13	0.19285252630088934
1104	14	-1.8076752899182935
1104	15	-0.6001887648836615
1104	16	-0.16666137173920959
1104	17	0.25863303205809984
1104	18	0.32442187664563704
1104	19	0.2753153507897963
1104	20	0.5475338920433899
1104	21	0.5948450012404933
1104	22	0.663639347855657
1104	23	1.2145275993263809
1104	24	0.6932415153880456
1104	25	0.6200713317879831
1105	1	-0.1498397904665012
1105	2	-0.06035254136257251
1105	3	-1.4486316718628984
1105	4	-0.3229099883693394
1105	5	-0.327454321842076
1105	6	-0.04600222432070211
1105	7	-0.06862170523763922
1105	8	0.03894780709111245
1105	9	0.28440734860802763
1105	10	0.4077866043504912
1105	11	-0.04916697058727948
1105	12	-0.08691163226610144
1105	13	-0.12367336906613957
1105	14	0.7500515002037744
1105	15	0.4977212717348891
1105	16	-0.24046678528298315
1105	17	0.33775957367522125
1105	18	0.23802177181194395
1105	19	0.28719485301186864
1105	20	-0.2650830750864799
1105	21	0.1763121886181196
1105	22	0.2570800631423822
1105	23	0.45144376001938014
1105	24	0.23163200545190676
1105	25	0.4803312135082862
1106	1	-1.6574160496221424
1106	2	-1.274997563480845
1106	3	-1.2331259386648554
1106	4	-1.4579724865225785
1106	5	-0.904663566431914
1106	6	-0.7665154277929105
1106	7	-0.7561745648418707
1106	8	-0.7952690033830052
1106	9	-0.5254923025543263
1106	10	-2.101495019742906
1106	11	-1.3646366813512572
1106	12	-1.203552317761364
1106	13	-1.0556501384455004
1106	14	-0.4097639751893425
1106	15	-1.0427632003084213
1106	16	-1.5390383597625004
1106	17	-0.7976110178383706
1106	18	-0.5752752648681039
1106	19	-0.6445677467564508
1106	20	-0.5421538829619692
1106	21	-0.4061851610237781
1106	22	-0.44772837090236095
1106	23	-0.7434453802615693
1106	24	-1.0427065818190542
1106	25	-1.7670556569487204
1107	1	0.021676974711024144
1107	2	0.14735314314184578
1107	3	-0.011300062092569618
1107	4	0.04049487338486557
1107	5	-0.0610904894051032
1107	6	-0.03727463311572876
1107	7	-0.04990625722023623
1107	8	-0.18391963182059357
1107	9	0.41496390934003813
1107	10	-0.011656087318620223
1107	11	-0.04489070501628589
1107	12	-0.11175758358182009
1107	13	0.009816987838846103
1107	14	-1.1544416845501146
1107	15	-0.5951642629199085
1107	16	-0.2879735173627367
1107	17	-0.03346651992556815
1107	18	0.22731371738700545
1107	19	0.14604369320365862
1107	20	0.2336273649810181
1107	21	0.4550943121878035
1107	22	-0.28900613481551574
1107	23	0.48267195973134136
1107	24	0.3685575952352309
1107	25	0.047555272356703426
1108	1	-0.04074760509569231
1108	2	0.1558028482138205
1108	3	-0.00443502967878473
1108	4	-0.15088818892340328
1108	5	-0.24043840234369185
1108	6	-0.11820252062697989
1108	7	-0.23005464703253153
1108	8	-0.32387565966257625
1108	9	0.3282453009922719
1108	10	0.3239378341998034
1108	11	0.06063280169242091
1108	12	0.07330397035022429
1108	13	-0.007674489221216163
1108	14	0.3595771142803912
1108	15	0.03301302768815617
1108	16	0.06990332767217211
1108	17	0.16354871785222969
1108	18	0.354620840850167
1108	19	0.18232540963658295
1108	20	0.3937880443121671
1108	21	0.4201412768220365
1108	22	0.3050400450822295
1108	23	0.1314659155181488
1108	24	0.18343896737418675
1108	25	-0.688171469206182
1109	1	-0.9237460949676084
1109	2	-0.8572878501641163
1109	3	-0.8858735408057079
1109	4	-0.8773663158045935
1109	5	-0.9927543885802805
1109	6	-1.33855163351042
1109	7	-0.9793514727201682
1109	8	-1.558167984182468
1109	9	-1.1039505567160433
1109	10	-1.3184613399469995
1109	11	-1.392326434588142
1109	12	-1.3534971815104768
1109	13	-1.3247766136871775
1109	14	-0.8492672694857077
1109	15	-1.4384562642437615
1109	16	-1.4468858758554717
1109	17	-1.4419340027156409
1109	18	-1.986495302668608
1109	19	-2.101978084109561
1109	20	-1.8091188152970474
1109	21	-1.661251766119527
1109	22	-1.7028484720401593
1109	23	-1.284375151565989
1109	24	-2.2311085389667085
1109	25	-1.282960888205551
1110	1	0.15248970908852755
1110	2	0.3342684183609954
1110	3	0.06880992550116716
1110	4	0.25510507983330655
1110	5	0.1628427175187923
1110	6	0.17618452644028082
1110	7	0.094183782089722
1110	8	0.0672949443517794
1110	9	0.37450985399691206
1110	10	0.319905157094297
1110	11	0.22356027407762014
1110	12	0.2272887611851857
1110	13	0.2288593539742223
1110	14	-2.865534307274274
1110	15	-0.7913931912269447
1110	16	-0.1913960693133916
1110	17	-0.22737525886412158
1110	18	-2.759411194689136
1110	19	-0.3878729730385828
1110	20	-1.9535567674690668
1110	21	-0.195051126571677
1110	22	-1.6762997994737887
1110	23	-0.38938423484531626
1110	24	0.10600967114919513
1110	25	-0.09816342939902256
1111	1	-0.14179694834845336
1111	2	-1.0671112023524714
1111	3	-0.24460250057498456
1111	4	0.10949760308896828
1111	5	-0.04177814149406345
1111	6	0.01940303595853976
1111	7	-0.04368641933711359
1111	8	0.02166778951312409
1111	9	-1.7378149207238351
1111	10	-0.2962261062742897
1111	11	-0.15019048713860172
1111	12	-0.019994672071725006
1111	13	0.011263353401346876
1111	14	0.9511182509362115
1111	15	0.33251388857878794
1111	16	0.3871892736501792
1111	17	-0.03932248198227484
1111	18	0.05712288742091687
1111	19	-0.055742487478696034
1111	20	0.44492406036157917
1111	21	-0.5028749738603508
1111	22	-0.15383894316230595
1111	23	0.04135539369281105
1111	24	-0.4967966696218369
1111	25	-1.868986753879149
1112	1	0.07025718548472426
1112	2	0.353644276684431
1112	3	-0.04844722599784035
1112	4	0.23805064261915732
1112	5	0.004001023133248793
1112	6	-0.07141498222418363
1112	7	0.05686462520603186
1112	8	-0.009158331725402036
1112	9	-0.31139608782570605
1112	10	0.3476837513641087
1112	11	0.08097404233178906
1112	12	-0.05377244852247287
1112	13	-0.03903352896631418
1112	14	0.5081004414393249
1112	15	0.25093261904032715
1112	16	0.2154584650648914
1112	17	-3.3684015770399824
1112	18	-0.8356165288237349
1112	19	-1.2542548371742441
1112	20	-0.31881800338658906
1112	21	-0.6655812566914463
1112	22	-1.6705557448071673
1112	23	-1.4040973519178621
1112	24	-0.25267965350322896
1112	25	-0.06284133939796988
1113	1	-0.31138283619824136
1113	2	-0.06151780657674166
1113	3	-0.10457194590410879
1113	4	-0.4262252134780482
1113	5	-0.19966444952712692
1113	6	-0.2294356693992441
1113	7	-0.3452448251740847
1113	8	-0.38548043818929756
1113	9	0.2312454213581887
1113	10	0.522045080227727
1113	11	-0.15492750162967184
1113	12	0.04407762225200429
1113	13	-0.37303017049228515
1113	14	-1.6910066603946206
1113	15	-1.806080316076887
1113	16	-0.5048890411289833
1113	17	-0.26925701156520176
1113	18	-0.5665846237622532
1113	19	-0.1271729654235969
1113	20	0.2532984559635878
1113	21	-1.4594175821091113
1113	22	-2.072728370902361
1113	23	-0.677656316065864
1113	24	-2.4695686758612467
1113	25	-0.5791274754652065
1114	1	0.1826494008722186
1114	2	0.32851169706782457
1114	3	0.014307566418001016
1114	4	0.41148912182219277
1114	5	0.21605665425627762
1114	6	0.16887611470349384
1114	7	0.14130151290297732
1114	8	0.03743369752746872
1114	9	0.4683935470090006
1114	10	0.3978230219146397
1114	11	0.2836680381134684
1114	12	0.26092789125581817
1114	13	0.11760806276006264
1114	14	1.1044818852492497
1114	15	0.46596910252776524
1114	16	0.35536738025248127
1114	17	-0.40059422086752505
1114	18	-0.11607134712220102
1114	19	-0.34158441110218263
1114	20	-1.6306708689236018
1114	21	-0.8472868115433414
1114	22	-1.3365655802046845
1114	23	-2.794596831511889
1114	24	-1.8256726762083488
1114	25	-1.2062055299601724
1115	1	0.27218341562298676
1115	2	0.48552482937939345
1115	3	0.3106333433316344
1115	4	0.4735679156916106
1115	5	0.4427221985031551
1115	6	0.2503937710909696
1115	7	0.44327114239582244
1115	8	0.4146677655026565
1115	9	0.5974601644340254
1115	10	0.8207553617254248
1115	11	0.47632415403408856
1115	12	0.48342183562000685
1115	13	0.32702302859867416
1115	14	1.178960557462844
1115	15	0.641512806295836
1115	16	0.7030625455197494
1115	17	0.5840533574212543
1115	18	0.6715976404189377
1115	19	0.029992986983913283
1115	20	0.7858846419765442
1115	21	-0.26338328895554497
1115	22	0.8147657907043637
1115	23	0.831783690521938
1115	24	0.8124545844718369
1115	25	0.9626095206030243
1116	1	0.6461756439210475
1116	2	0.706619455172536
1116	3	0.6253737208836192
1116	4	0.8560551837869141
1116	5	0.8547652328983659
1116	6	0.4459197860097177
1116	7	0.4919083187925608
1116	8	0.4891601995788122
1116	9	0.7893424973958716
1116	10	0.7404620181166592
1116	11	0.6618824443121198
1116	12	0.5819736955239208
1116	13	0.13121211538763333
1116	14	1.2458516603088299
1116	15	0.940227299739675
1116	16	0.846237215165587
1116	17	0.9935822490805606
1116	18	0.9078558057001658
1116	19	1.1236375366402527
1116	20	0.8880464156853378
1116	21	0.469293375852601
1116	22	0.3615837488917464
1116	23	-0.4549956736182992
1116	24	0.12872840768452676
1116	25	-0.719353281100112
1201	1	0.49689482393011275
1201	2	0.7066413358605033
1201	3	0.5577909904655023
1201	4	0.5211971871363943
1201	5	0.5575559120494518
1201	6	0.3511720078257099
1201	7	0.4844039374452791
1201	8	0.4887449295908013
1201	9	0.6544004742727338
1201	10	1.0465810624886132
1201	11	0.6396799134227251
1201	12	0.717048623566555
1201	13	0.6625313680544664
1201	14	1.4127005888613198
1201	15	0.9733218981734808
1201	16	0.6211368013311613
1201	17	0.836105432769287
1201	18	0.6604699690579068
1201	19	1.0102265735588816
1201	20	1.0340743067320162
1201	21	0.9085122978084712
1201	22	0.9629594074252026
1201	23	0.6685352813035479
1201	24	0.8781719551622587
1201	25	1.256209116254574
1202	1	0.4984518105666851
1202	2	0.7775443967493558
1202	3	0.5798308367342067
1202	4	0.37634633263275674
1202	5	0.32192014744312303
1202	6	0.40433319673404355
1202	7	0.3684692807741001
1202	8	0.3722918944912763
1202	9	0.9062556827849022
1202	10	0.05412297492733842
1202	11	0.508806824680903
1202	12	0.3399480029811812
1202	13	0.5549300766010106
1202	14	1.3115582309133629
1202	15	0.9763116457589831
1202	16	0.8306969584689412
1202	17	-1.4617769666662213
1202	18	0.24064540066040507
1202	19	0.569206251766829
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2210	19	0.6866538120592196
2210	20	0.7065007789907938
2210	21	0.8674241487003442
2210	22	0.9771145483823176
2210	23	1.333835396168034
2210	24	0.9914245620934921
2210	25	0.923517625816686
2211	1	-0.0010945817706726757
2211	2	-0.7472527615395386
2211	3	-0.15039489928588878
2211	4	0.19522112625187305
2211	5	0.14898860444499462
2211	6	0.10998376041958835
2211	7	0.1691366203678868
2211	8	0.21341625001690545
2211	9	0.6310924870501324
2211	10	0.7744469738209858
2211	11	0.368289594377174
2211	12	0.27013732918381805
2211	13	0.24731244949814624
2211	14	-1.0342875228462134
2211	15	-0.1290425366794139
2211	16	0.18587769733613158
2211	17	0.13617301758931433
2211	18	0.19921511297678762
2211	19	0.4123213904210843
2211	20	0.6353788067606274
2211	21	0.5035740377797779
2211	22	-0.099574823769224
2211	23	0.44489804949699163
2211	24	-0.06961486437929665
2211	25	0.3029370107770504
2212	1	-0.09267720491230946
2212	2	-1.075234951871824
2212	3	-0.29325591301792997
2212	4	0.10783771928083752
2212	5	-0.006053369500833128
2212	6	0.009575314808110136
2212	7	-0.01876452750590829
2212	8	-0.017990116251660027
2212	9	-2.2121851239005252
2212	10	-0.054433031789507424
2212	11	0.055725098770388115
2212	12	-0.07348570042701397
2212	13	0.008356738682557818
2212	14	0.541967118788186
2212	15	0.18532373734392138
2212	16	0.2786541438365404
2212	17	0.335065856288594
2212	18	0.15568689703115268
2212	19	-0.5793874232422809
2212	20	0.26066650384507994
2212	21	0.2895818268908741
2212	22	-1.5341857279782758
2212	23	-0.45622386666805426
2212	24	0.22132760713333965
2212	25	-0.4691479244153953
2213	1	0.20835088457451434
2213	2	0.5641654962116966
2213	3	0.21980181412557798
2213	4	0.3564418483375533
2213	5	0.32207658311310894
2213	6	0.21063562009952363
2213	7	0.2728768572457767
2213	8	0.33376527511901344
2213	9	0.8319964168520855
2213	10	0.9010412363197631
2213	11	0.22549835781924799
2213	12	0.23062945169188964
2213	13	0.09425912689304994
2213	14	-1.8766071314074608
2213	15	-0.5080710950068905
2213	16	0.09465496198765777
2213	17	-1.82450978083223
2213	18	-0.8281393222823823
2213	19	-0.44030410811340204
2213	20	0.44031362537343455
2213	21	0.05452098131932814
2213	22	0.5562782282020464
2213	23	0.9040466798255267
2213	24	0.7385805123539342
2213	25	-0.7156149539635948
2214	1	0.1805441807015704
2214	2	0.09857044917097468
2214	3	0.029294198817144235
2214	4	0.437957298220077
2214	5	0.4157610424230924
2214	6	0.22555942507685955
2214	7	0.27866180650013916
2214	8	0.1001977144506867
2214	9	0.6178932012482505
2214	10	0.619458497512646
2214	11	-0.016071143930929566
2214	12	-0.08432233607506845
2214	13	0.07333963262279042
2214	14	0.9316185505909544
2214	15	0.581888690010679
2214	16	0.4434691809309399
2214	17	0.18902266532545298
2214	18	0.287479207456272
2214	19	-0.9254739745724194
2214	20	0.009018382000697045
2214	21	0.4399081158469045
2214	22	0.6730864265689576
2214	23	1.166844419107484
2214	24	0.8771729620333346
2214	25	0.925797323246691
2215	1	-0.14042665612199556
2215	2	0.38265398293740915
2215	3	0.21776526723719414
2215	4	0.45686752103683403
2215	5	0.26171819425372433
2215	6	0.2090259768768874
2215	7	0.21670010080418223
2215	8	0.19915616964323135
2215	9	0.46117209819342264
2215	10	0.73837210981862
2215	11	0.4442401988021061
2215	12	0.235143388845934
2215	13	0.08454359762159669
2215	14	1.1076643210919315
2215	15	0.5081897471236871
2215	16	0.5516983637853912
2215	17	0.5761251730281671
2215	18	0.31974033236576105
2215	19	-0.32577628127339864
2215	20	-1.5765077842697792
2215	21	-2.052199595308082
2215	22	-0.06733247297724887
2215	23	0.539217055420373
2215	24	0.6518337436830564
2215	25	-0.0346806740264185
2216	1	0.03280684190113092
2216	2	-0.24810498392006153
2216	3	0.015473406149684621
2216	4	0.22959594888652646
2216	5	0.24118041731861695
2216	6	0.008051766780994285
2216	7	0.0569228228788603
2216	8	0.11097639786286351
2216	9	0.40574809422745517
2216	10	-1.554784456534236
2216	11	-0.35008754023284894
2216	12	-0.037754371681785415
2216	13	0.21999268454125342
2216	14	-0.3474800295441085
2216	15	-0.2894645532640112
2216	16	0.16387252203256963
2216	17	0.4010796988042884
2216	18	0.25353338719606455
2216	19	-1.37836213652281
2216	20	-0.9810811025904798
2216	21	-0.8346280538861066
2216	22	-0.6698976200084417
2216	23	-4.221031124828961
2216	24	-1.8366328447367763
2216	25	-1.1456051474449964
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	21.0
5	42.0
6	40.0
7	230.0
8	270.0
9	695.0
10	1384.0
11	2626.0
12	4555.0
13	7167.0
14	10454.0
15	15260.0
16	20891.0
17	24431.0
18	25097.0
19	24412.0
20	24535.0
21	23812.0
22	23030.0
23	23334.0
24	24183.0
25	24755.0
26	26435.0
27	29824.0
28	34416.0
29	41868.0
30	51363.0
31	63919.0
32	85241.0
33	115656.0
34	156507.0
35	217228.0
36	311932.0
37	461937.0
38	709513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.73880431599629	16.991382475055907	11.433258764425563	19.836554444522243
2	9.566648483580906	25.521320409038246	38.452161925082336	26.45986918229851
3	21.429074062761224	32.969323075471685	32.12493630078249	13.476666560984608
4	12.335086226896605	21.088005568014847	30.444281184749826	36.13262702033872
5	7.163070269534503	50.02784207664769	32.67522913642386	10.133858517393952
6	28.45234514598351	27.90306580450016	28.81141795874374	14.833171090772588
7	20.33636524957734	41.680001453649645	19.07385506726416	18.909778229508856
8	19.32383568338692	38.665341233348954	27.85621600387268	14.154607079391447
9	28.844261480576716	24.822420265529598	15.83901808875449	30.494300165139197
10	12.751372193030647	27.67265932691114	28.400577911993295	31.17539056806491
11	24.922608459310048	31.212403678606375	20.14766541801484	23.717322444068735
12	28.25602114218358	27.728974278582136	21.591988485613008	22.423016093621275
13	27.75191910287861	20.117765547584664	35.278900597140144	16.851414752396582
14	33.17098803405848	25.409037075588387	21.483905118478173	19.936069771874955
15	35.611281024169756	20.09484628560602	23.856257418928273	20.43761527129595
16	25.091446004811914	22.508031126244394	33.84714241142177	18.553380457521925
17	18.933522830299047	36.24701671797802	27.82516275874281	16.99429769298012
18	20.106261560255607	21.979680241010065	42.5195867073299	15.394471491404428
19	22.32146757693695	36.70477157352556	19.86135633571236	21.112404513825126
20	18.133363951542815	34.3760534674349	29.546185558720467	17.944397022301807
21	19.68818135428103	36.16960985943806	23.335214708747625	20.806994077533286
22	17.224982119730154	33.07761003614714	17.912666963060328	31.78474088106238
23	24.201237534287355	24.72083955934346	21.459988904331755	29.617934002037426
24	15.899135948040902	33.24485338178725	17.512071598399473	33.34393907177237
25	23.22453152303961	34.848463742274596	19.985474002566235	21.941530732119556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13499.0
1	13499.0
2	12763.0
3	12027.0
4	12027.0
5	12027.0
6	17917.0
7	23807.0
8	23807.0
9	23807.0
10	20822.0
11	17837.0
12	17837.0
13	17837.0
14	17336.0
15	16835.0
16	16835.0
17	16835.0
18	19328.0
19	21821.0
20	21821.0
21	21821.0
22	27115.5
23	32410.0
24	32410.0
25	32410.0
26	39383.0
27	46356.0
28	46356.0
29	46356.0
30	52970.5
31	59585.0
32	59585.0
33	59585.0
34	70579.0
35	81573.0
36	81573.0
37	81573.0
38	168277.5
39	254982.0
40	254982.0
41	254982.0
42	638990.5
43	1022999.0
44	1022999.0
45	1022999.0
46	752271.0
47	481543.0
48	481543.0
49	481543.0
50	353286.5
51	225030.0
52	225030.0
53	225030.0
54	164606.5
55	104183.0
56	104183.0
57	104183.0
58	85548.0
59	66913.0
60	66913.0
61	66913.0
62	58480.5
63	50048.0
64	50048.0
65	50048.0
66	38304.5
67	26561.0
68	26561.0
69	26561.0
70	21447.5
71	16334.0
72	16334.0
73	16334.0
74	12045.0
75	7756.0
76	7756.0
77	7756.0
78	5477.0
79	3198.0
80	3198.0
81	3198.0
82	2137.5
83	1077.0
84	1077.0
85	1077.0
86	781.5
87	486.0
88	486.0
89	486.0
90	318.0
91	150.0
92	150.0
93	150.0
94	101.5
95	53.0
96	53.0
97	53.0
98	32.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07703683775570394
2	0.016273210584621854
3	0.027057594796283366
4	0.03378333978850238
5	0.04097292926294339
6	0.01592532722295535
7	0.018592432995731857
8	0.028990280138875037
9	0.029802007982763543
10	0.02013858126980519
11	0.021298192475360196
12	0.015345521620177853
13	0.022380496267211527
14	0.031580078497947875
15	0.030304506171837373
16	0.022225881439804196
17	0.022303188853507863
18	0.024197220489247696
19	0.01712359213536219
20	0.01461110118999302
21	0.012137263951475684
22	0.016389171705177355
23	0.01851512558202819
24	0.016389171705177355
25	0.10598846418772714
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2587074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.869708126443937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.80379320136687	11.420499879080847
2	8.134443515448895	2.4191360169153926
3	3.691481790845529	1.6467377035186674
4	2.20782111130019	1.3131862208173968
5	1.4814350319182787	1.1014253266456984
6	1.0887182707511263	0.9713353750797609
7	0.8180266449049243	0.8514672214573294
8	0.671175077947689	0.7984142008660317
9	0.5333868540138325	0.7138176153901065
>10	3.9258684598021754	11.28451622670952
>50	0.33081138602793775	3.386261177552675
>100	0.23412573915086604	7.071983381834531
>500	0.03292393205871383	3.388946066200846
>1k	0.03318523310679886	10.991801256553908
>5k	0.006009924105955699	6.297026325826422
>10k+	0.00679382725021079	36.34344600555088
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	195454	7.555021619018243	No Hit
GCTTATGTACTCTGCGTTGATACCA	119521	4.6199296966379775	No Hit
CTTATGTACTCTGCGTTGATACCAC	73492	2.840738223954939	No Hit
GAGTACATAAGCAGTGGTATCAACG	48192	1.8627994406035544	No Hit
TATGTACTCTGCGTTGATACCACTG	45581	1.7618746120134174	No Hit
GCGTTGATACCACTGCTTATGTACT	39880	1.5415098292511153	No Hit
CCCATGTACTCTGCGTTGATACCAC	36850	1.4243890974900602	No Hit
CATAAGCAGTGGTATCAACGCAGAG	31671	1.2242015497044152	No Hit
CATGTACTCTGCGTTGATACCACTG	29425	1.1373853241151972	No Hit
ACTCTGCGTTGATACCACTGCTTAT	28652	1.1075060087187303	No Hit
GTACATGATAAGCAGTGGTATCAAC	27385	1.0585317621374573	No Hit
CTGCTTATGTACTCTGCGTTGATAC	26956	1.0419493218980207	No Hit
ATCATGTACTCTGCGTTGATACCAC	26894	1.039552792073207	No Hit
GTATCAACGCAGAGTACATAAGCAG	26208	1.0130363491728493	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	26037	1.0064265653011857	No Hit
TATCAACGCAGAGTACATAAGCAGT	21131	0.8167914794860911	No Hit
GGTATCAACGCAGAGTACATAAGCA	18597	0.7188429863235454	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	16635	0.6430044134802484	No Hit
GTGGTATCAACGCAGAGTACATAAG	14535	0.5618316290913982	No Hit
GTACATGGGAAGCAGTGGTATCAAC	13432	0.519196590433826	No Hit
GCAGTGGTATCAACGCAGAGTACAT	12781	0.49403302727328247	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	12072	0.46662754911533266	No Hit
GTACTCTGCGTTGATACCACTGCTT	11740	0.45379451844052393	No Hit
ACATAAGCAGTGGTATCAACGCAGA	11176	0.4319938277760899	No Hit
CCACTGCTTATGTACTCTGCGTTGA	10550	0.4077966072868422	No Hit
GAGTACATGATAAGCAGTGGTATCA	10521	0.406675649788139	No Hit
GTACATGGGATAAGCAGTGGTATCA	9531	0.36840848000482396	No Hit
GATACCACTGCTTATGTACTCTGCG	9490	0.3668236780238988	No Hit
ATACCACTGCTTATGTACTCTGCGT	9473	0.3661665650074176	No Hit
GTTGATACCACTGCTTATGTACTCT	9201	0.35565275674371893	No Hit
GCTTATCATGTACTCTGCGTTGATA	8688	0.33582340512872844	No Hit
GCTTATCCCATGTACTCTGCGTTGA	8376	0.32376344859095646	No Hit
GCGTTGATACCACTGCTTATCATGT	8040	0.3107758030887404	No Hit
CAGTGGTATCAACGCAGAGTACATA	7797	0.3013829523237449	No Hit
GATAAGCAGTGGTATCAACGCAGAG	7235	0.27965956907301454	No Hit
TACCACTGCTTATGTACTCTGCGTT	7168	0.2770697707139417	No Hit
CCCCATGTACTCTGCGTTGATACCA	6979	0.2697642201189452	No Hit
TGATACCACTGCTTATGTACTCTGC	6938	0.26817941813802004	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6275	0.2425520104952545	No Hit
GCAGAGTACATAAGCAGTGGTATCA	6195	0.2394597139471078	No Hit
GTGGTATCAACGCAGAGTACATGGG	6182	0.23895721575803397	No Hit
ACGCAGAGTACATGATAAGCAGTGG	6119	0.23652203222636847	No Hit
GTATCAACGCAGAGTACATGATAAG	5804	0.22434611456804096	No Hit
TATCAACGCAGAGTACATGATAAGC	5799	0.22415284603378177	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5669	0.21912786414304344	No Hit
GGATAAGCAGTGGTATCAACGCAGA	5537	0.21402557483860143	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	5389	0.20830482622453012	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5118	0.19782967166768325	No Hit
GGGATAAGCAGTGGTATCAACGCAG	5063	0.19570371779083243	No Hit
CAGTGGTATCAACGCAGAGTACATG	4951	0.1913745026234271	No Hit
GTACATGGTAAGCAGTGGTATCAAC	4905	0.18959643210824276	No Hit
ATCAACGCAGAGTACATAAGCAGTG	4884	0.18878470426435426	No Hit
GCGTTGATACCACTGCTTCCCATGT	4846	0.18731586340398457	No Hit
ATGATAAGCAGTGGTATCAACGCAG	4818	0.18623355961213325	No Hit
GTACACAAGCAGTGGTATCAACGCA	4786	0.18499664099287458	No Hit
GTATCAACGCAGAGTACATGGGAAG	4750	0.18360510754620857	No Hit
CTGCTTATCATGTACTCTGCGTTGA	4605	0.17800032005269273	No Hit
GAGTACATGGGATAAGCAGTGGTAT	4574	0.1768020551402859	No Hit
CATGTAAGCAGTGGTATCAACGCAG	4563	0.17637686436491573	No Hit
GCTTACATGTACTCTGCGTTGATAC	4559	0.1762222495375084	No Hit
GGTATCAACGCAGAGTACATGATAA	4551	0.17591301988269373	No Hit
GAGTACATGTAAGCAGTGGTATCAA	4523	0.1748307160908424	No Hit
ACCATGTACTCTGCGTTGATACCAC	4386	0.16953515825214122	No Hit
GTACATAAGCAGTTGTATCAACGCA	4290	0.16582440239436522	No Hit
AAAAAGTACTCTGCGTTGATACCAC	3968	0.1533779087880749	No Hit
TACATGTACTCTGCGTTGATACCAC	3928	0.15183176051400152	No Hit
GCGTTGATACCACTGCTTATCCCAT	3832	0.14812100465622552	No Hit
CCATGTACTCTGCGTTGATACCACT	3808	0.1471933156917815	No Hit
ATGTACTCTGCGTTGATACCACTGC	3773	0.14584043595196736	No Hit
TATCAACGCAGAGTACATGGGAAGC	3763	0.14545389888344903	No Hit
GTACATGTAAGCAGTGGTATCAACG	3689	0.14259352457641336	No Hit
CATGATAAGCAGTGGTATCAACGCA	3676	0.14209102638733953	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3538	0.13675681484178653	No Hit
GGTATCAACGCAGAGTACATGGGAA	3446	0.13320067381141784	No Hit
GTATCAACGCAGAGTACATGGGATA	3402	0.13149991070993716	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3397	0.131306642175678	No Hit
TACATAAGCAGTGGTATCAACGCAG	3343	0.129219342005679	No Hit
TACCACTGCTTATCATGTACTCTGC	3325	0.128523575282346	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3241	0.12527666390679199	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3205	0.123885130460126	No Hit
CTGCGTTGATACCACTGCTTATGTA	3103	0.11994245236123899	No Hit
GTGGTATCAACGCAGAGTACATGAT	3075	0.11886014856938765	No Hit
GCTTATGTACTCTTCGTTGATACCA	2993	0.11569054460753732	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2889	0.11167055909494664	No Hit
ACGCAGAGTACATGGGATAAGCAGT	2885	0.11151594426753932	No Hit
AAAGTACTCTGCGTTGATACCACTG	2744	0.1060657716014308	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2708	0.10467423815476481	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2653	0.10254828427791397	No Hit
GGTATCAACGCAGAGTACATGGGAT	2623	0.10138867307235896	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.865370685183338E-5	0.0	0.0	0.0	0.0
6	7.730741370366677E-5	0.0	0.0	3.865370685183338E-5	0.0
7	7.730741370366677E-5	0.0	0.0	3.865370685183338E-5	0.0
8	7.730741370366677E-5	0.0	0.0	3.865370685183338E-5	0.0
9	7.730741370366677E-5	0.0	0.0	3.865370685183338E-5	0.0
10	7.730741370366677E-5	0.0	0.0	3.865370685183338E-5	0.0
11	7.730741370366677E-5	0.0	0.0	3.865370685183338E-5	0.0
12	7.730741370366677E-5	0.0	0.0	1.1596112055550015E-4	0.0
13	7.730741370366677E-5	0.0	0.0	1.1596112055550015E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACTTC	25	0.006040092	18.99621	6
AGTTGTA	625	0.0	16.260756	11
TTACCAC	165	0.0	15.558897	19
CAGTTGT	665	0.0	15.425495	10
GCAGTTG	675	0.0	15.196969	9
TTGTATC	650	0.0	15.196967	13
GTTGTAT	670	0.0	14.88509	12
CGCAAAA	295	0.0	14.81662	2
TATAGGT	45	6.75389E-4	14.780831	4
ATACCGT	45	6.774716E-4	14.77483	6
TAACGCA	150	0.0	14.579264	19
AGCAGTT	720	0.0	14.379354	8
GCGCTAC	60	2.5743986E-5	14.247158	13
GCAGACT	40	0.005287351	14.247157	9
CTTCGTT	415	0.0	14.189941	12
TTCGTTG	425	0.0	14.079545	13
TGTATCA	710	0.0	13.912717	14
GTACAAA	700	0.0	13.853755	1
CTCTTCG	430	0.0	13.694942	10
TCGTTGA	430	0.0	13.25317	14
>>END_MODULE