FastQCFastQC Report
Wed 25 May 2016
SRR1295317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587074
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA2083568.053731744820597No Hit
GCTTATGTACTCTGCGTTGATACCA1337435.169662715484753No Hit
CTTATGTACTCTGCGTTGATACCAC861013.328122813649706No Hit
GAGTACATAAGCAGTGGTATCAACG521492.0157521586162592No Hit
GCGTTGATACCACTGCTTATGTACT455621.7611401915832325No Hit
CCCATGTACTCTGCGTTGATACCAC441431.706290581560481No Hit
TATGTACTCTGCGTTGATACCACTG415301.6052884455566403No Hit
CATAAGCAGTGGTATCAACGCAGAG386751.4949321124946562No Hit
CATGTACTCTGCGTTGATACCACTG340071.3144966089102978No Hit
ACTCTGCGTTGATACCACTGCTTAT334071.2913043847991978No Hit
CTGCTTATGTACTCTGCGTTGATAC313661.212412169114606No Hit
ACGCAGAGTACATAAGCAGTGGTAT308561.192698778620171No Hit
ATCATGTACTCTGCGTTGATACCAC306691.1854705354388781No Hit
GTATCAACGCAGAGTACATAAGCAG284381.0992341154524377No Hit
GTACATGATAAGCAGTGGTATCAAC279111.0788636119415216No Hit
TATCAACGCAGAGTACATAAGCAGT226480.8754291527803225No Hit
GGTATCAACGCAGAGTACATAAGCA204820.7917052237392515No Hit
AAGCAGTGGTATCAACGCAGAGTAC183210.7081745632324394No Hit
GTACTTTTTTTTTTTTTTTTTTTTT162160.6268085103093302No Hit
GTGGTATCAACGCAGAGTACATAAG153520.5934117075893461No Hit
GTATCAACGCAGAGTACTTTTTTTT152650.5900488350932366No Hit
GCAGTGGTATCAACGCAGAGTACAT145070.5607493252995469No Hit
ACATAAGCAGTGGTATCAACGCAGA137240.5304834728345613No Hit
GTACATGGGAAGCAGTGGTATCAAC131270.5074072098440168No Hit
CCACTGCTTATGTACTCTGCGTTGA123210.4762523221214391No Hit
GTACTCTGCGTTGATACCACTGCTT119060.4602110337779282No Hit
GAGTACATGATAAGCAGTGGTATCA110520.42720076812646257No Hit
GATACCACTGCTTATGTACTCTGCG110180.4258865420935002No Hit
ATACCACTGCTTATGTACTCTGCGT105090.40621180530591705No Hit
GGTATCAACGCAGAGTACTTTTTTT101740.39326281351055287No Hit
GCTTATCATGTACTCTGCGTTGATA97530.376989602925931No Hit
GTACATGGGATAAGCAGTGGTATCA96720.3738586526709325No Hit
TATCAACGCAGAGTACTTTTTTTTT96190.3718100062077853No Hit
CAGTGGTATCAACGCAGAGTACATA95230.36809925035000934No Hit
GCTTATCCCATGTACTCTGCGTTGA94480.3652002223361218No Hit
GCGTTGATACCACTGCTTATCATGT91250.35271507502297966No Hit
GTTGATACCACTGCTTATGTACTCT91040.35190334717909116No Hit
CCCCATGTACTCTGCGTTGATACCA85390.33006400280780523No Hit
GATAAGCAGTGGTATCAACGCAGAG82310.31815866109744056No Hit
TACCACTGCTTATGTACTCTGCGTT81560.3152596330835531No Hit
TGATACCACTGCTTATGTACTCTGC76390.29527566664115523No Hit
GCAGAGTACATAAGCAGTGGTATCA75140.29044395328467604No Hit
ACGCAGAGTACATGATAAGCAGTGG72820.2814762932950507No Hit
ACGCAGAGTACTTTTTTTTTTTTTT66590.2573950339263585No Hit
GTGGTATCAACGCAGAGTACATGGG65800.25434139108506365No Hit
GGATAAGCAGTGGTATCAACGCAGA63980.24730641643802997No Hit
TATCAACGCAGAGTACATGATAAGC61320.2370245304154423No Hit
GGGATAAGCAGTGGTATCAACGCAG60980.23571030438247997No Hit
GTATCAACGCAGAGTACATGATAAG59880.23145839662877832No Hit
ATCAACGCAGAGTACATAAGCAGTG59610.2304147465437788No Hit
GAGTACATGGGAAGCAGTGGTATCA59000.228056870425817No Hit
CAGTGGTATCAACGCAGAGTACATG58690.2268586055134101No Hit
GCGTTGATACCACTGCTTCCCATGT55970.21634479724971142No Hit
ATGATAAGCAGTGGTATCAACGCAG54290.20985097449860343No Hit
CATGTAAGCAGTGGTATCAACGCAG52920.20455541665990226No Hit
GTACACAAGCAGTGGTATCAACGCA52520.20300926838582892No Hit
ACCATGTACTCTGCGTTGATACCAC52070.2012698515774964No Hit
GAGTACATGTAAGCAGTGGTATCAA51830.20034216261305243No Hit
GCTTACATGTACTCTGCGTTGATAC51610.19949178106231208No Hit
CTGCTTATCATGTACTCTGCGTTGA51570.19933716623490474No Hit
GTACATGGTAAGCAGTGGTATCAAC50730.19609025485935075No Hit
GTATCAACGCAGAGTACATGGGAAG50020.19334584167287058No Hit
GAGTACATGGGATAAGCAGTGGTAT49050.18959643210824276No Hit
CCATGTACTCTGCGTTGATACCACT48300.18669740409435523No Hit
GGTATCAACGCAGAGTACATGATAA47280.18275472599546824No Hit
TACATGTACTCTGCGTTGATACCAC45680.1765701328991749No Hit
AAAAAGTACTCTGCGTTGATACCAC43870.16957381195899307No Hit
GCGTTGATACCACTGCTTATCCCAT43100.1665974765314019No Hit
CATGATAAGCAGTGGTATCAACGCA42950.16601767092862438No Hit
TACATAAGCAGTGGTATCAACGCAG42880.16574709498066156No Hit
ACGCAGAGTACATGGGAAGCAGTGG41830.16168845576121904No Hit
CTTATACACATCTCCGAGCCCACGA40040.15476944223474087No Hit
TATCAACGCAGAGTACATGGGAAGC39350.1521023364619644No Hit
ACTCTGCGTTGATACCACTGCTTCC38910.1504015733604837No Hit
TACCACTGCTTATCATGTACTCTGC38260.14788908241511453No Hit
GTACATGTAAGCAGTGGTATCAACG38240.14781177500141088No Hit
ATGTACTCTGCGTTGATACCACTGC38040.14703870086437418No Hit
CTGCGTTGATACCACTGCTTATGTA36810.1422842949215987No Hit
GGTATCAACGCAGAGTACATGGGAA36680.14178179673252486No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36370.14058353182011804No Hit
GTATCAACGCAGAGTACATGGGATA36010.13919199837345203No Hit
ACGCAGAGTACATGGGATAAGCAGT34130.13192510148530734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33960.13126798846882617No Hit
GTGGTATCAACGCAGAGTACATGAT31810.122957441495682No Hit
GCTTCCCATGTACTCTGCGTTGATA31410.12141129322160865No Hit
GTACTGGTTCACTATCGGTCAGTCA31360.12121802468734949No Hit
GCTTGTGTACTCTGCGTTGATACCA30930.11955591529272067No Hit
AAAGTACTCTGCGTTGATACCACTG29910.11561323719383365No Hit
GGTATCAACGCAGAGTACATGGGAT27990.10819172547828163No Hit
CTCTGCGTTGATACCACTGCTTATG27750.10726403651383765No Hit
TGATAAGCAGTGGTATCAACGCAGA27580.10660692349735647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAG250.00600613319.0147633
GCTCCGT250.00604651918.99271411
CTTATAC4950.017.8820761
AATCGCC702.5169356E-816.27946918
AAATCGC651.9230902E-716.07075717
TATACAC5800.015.7363563
TTAAGGT551.1206068E-515.5575344
GGTTATA952.9649527E-1015.0282311
TTATACA6100.014.6507212
GCATAGA954.778485E-914.0263481
ATCGCCG1001.0082658E-813.3000419
GTACAAA6100.013.1065881
GAAATCG957.431299E-812.99501516
CTCTTAG604.0292364E-412.6905071
CATAGAT751.4677138E-512.676512
TAGATCT751.4677138E-512.676514
TAAGCCT701.0835557E-412.2237774
TTATGTC1251.3951649E-912.1694493
TAGACGT550.003051588512.1003054
CCTATTC550.003051588512.1003053
Produced by FastQC (version 0.11.5)