FastQCFastQC Report
Wed 25 May 2016
SRR1295316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744571
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA620653.557608145498234No Hit
GCTTATGTACTCTGCGTTGATACCA375312.1513025265237125No Hit
CTTATGTACTCTGCGTTGATACCAC246861.4150183626805672No Hit
GAGTACATAAGCAGTGGTATCAACG166280.9531283048955875No Hit
TATGTACTCTGCGTTGATACCACTG160760.9214872882788948No Hit
CCCATGTACTCTGCGTTGATACCAC146690.8408370883156948No Hit
GCGTTGATACCACTGCTTATGTACT127250.7294056819699514No Hit
CATAAGCAGTGGTATCAACGCAGAG106040.6078285148612467No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA104620.599688977977967No Hit
CATGTACTCTGCGTTGATACCACTG103900.5955618888540506No Hit
ACTCTGCGTTGATACCACTGCTTAT103600.5938422683857522No Hit
GTACATGATAAGCAGTGGTATCAAC95150.5454062918620108No Hit
ATCATGTACTCTGCGTTGATACCAC93190.5341714381357938No Hit
CTGCTTATGTACTCTGCGTTGATAC92270.5288979353663451No Hit
ACGCAGAGTACATAAGCAGTGGTAT90730.520070550295746No Hit
GTATCAACGCAGAGTACTTTTTTTT81460.46693427782532215No Hit
GTATCAACGCAGAGTACATAAGCAG81010.46435484712287434No Hit
GTGGTATCAACGCAGAGTACATGGG72970.4182690185724743No Hit
TATCAACGCAGAGTACATAAGCAGT69160.39642983862508313No Hit
GGTATCAACGCAGAGTACATAAGCA64590.3702342868246692No Hit
GTACTGGTTCACTATCGGTCAGTCA62560.35859818832251594No Hit
GTACATGGGAAGCAGTGGTATCAAC59890.3432935661546592No Hit
AAGCAGTGGTATCAACGCAGAGTAC56420.3234032894046731No Hit
GGTATCAACGCAGAGTACTTTTTTT52740.3023092783268781No Hit
GTGGTATCAACGCAGAGTACATAAG51520.2953161550891308No Hit
TATCAACGCAGAGTACTTTTTTTTT47380.27158539262661135No Hit
ATACAGGGTGACAGCCCCGTACACA44620.25576488431826505No Hit
GCAGTGGTATCAACGCAGAGTACAT44560.25542096022460536No Hit
GGATACCACGTGTCCCGCCCTACTC44010.2522683226993914No Hit
GTACATGGGATAAGCAGTGGTATCA42420.24315433421740934No Hit
ACATAAGCAGTGGTATCAACGCAGA40280.2308877082102133No Hit
GTACTCTGCGTTGATACCACTGCTT39880.2285948809191486No Hit
GCTTATCCCATGTACTCTGCGTTGA37230.21340490011584506No Hit
GAGTACATGATAAGCAGTGGTATCA36950.2117999210120998No Hit
CCACTGCTTATGTACTCTGCGTTGA36260.20784479393501326No Hit
GATACCACTGCTTATGTACTCTGCG35470.2033164600351605No Hit
GTTGATACCACTGCTTATGTACTCT32330.18531776580030276No Hit
ACCCTGTATCGCGCGCCTTTCCAGA32150.18428599351932365No Hit
GCTTATCATGTACTCTGCGTTGATA31910.18291029714468485No Hit
ATACCACTGCTTATGTACTCTGCGT30840.17677698414108683No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30210.17316578115765996No Hit
GTTAATGATAGTGTGTCGAAACACA28900.16565677177942315No Hit
GGTTAATGAGGCGAACCGGGGGAAC27370.15688670739110072No Hit
GGTATCAACGCAGAGTACATGGGGG27030.15493780419369574No Hit
GATAAGCAGTGGTATCAACGCAGAG26950.15447923873548283No Hit
CCCATATTCAGACAGGATACCACGT26420.1514412425748221No Hit
GCGTTGATACCACTGCTTATCATGT26300.1507533943875027No Hit
GTATTTAGCCTTGGAGGATGGTCCC26130.14977894278880022No Hit
GGATAAGCAGTGGTATCAACGCAGA25740.14754343618001217No Hit
GAGTACATGGGAAGCAGTGGTATCA25710.1473714741331823No Hit
ATCATTAACTGAATCCATAGGTTAA25520.1462823811699266No Hit
CAGTGGTATCAACGCAGAGTACATA25120.1439895538788619No Hit
TGATACCACTGCTTATGTACTCTGC23960.13734035473477432No Hit
TACCACTGCTTATGTACTCTGCGTT23850.13670982722973155No Hit
GTATCAACGCAGAGTACATGGGAAG23760.13619394108924202No Hit
GTTCACTATCGGTCAGTCAGGAGTA23460.1344743206209435No Hit
GTATCAACGCAGAGTACATGGGGGT23280.13344254833996438No Hit
TCTAAGTACCCCGAGGAAAAGAAAT22780.13057651422613353No Hit
GGTATCAACGCAGAGTACATGGGAA22570.12937277989832457No Hit
ACGCAGAGTACATGATAAGCAGTGG22160.12702263192498328No Hit
GAGTACATGGGATAAGCAGTGGTAT22150.12696531124270666No Hit
GCCTTGGAGGATGGTCCCCCCATAT22020.12622014237311063No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21910.12558961486806786No Hit
TATTCAGACAGGATACCACGTGTCC21880.12541765282123798No Hit
ACTTAGATGTTTCAGTTCCCCCGGT21390.12260893938968377No Hit
GGTATCAACGCAGAGTACATGGGAT21320.12220769461374745No Hit
GCGTTGATACCACTGCTTCCCATGT21000.1203734327808957No Hit
CCCCATGTACTCTGCGTTGATACCA20830.1193989811821932No Hit
GGGATAAGCAGTGGTATCAACGCAG20830.1193989811821932No Hit
GCAGAGTACATAAGCAGTGGTATCA20780.11911237777081013No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG20260.11613170229242605No Hit
GTATCAACGCAGAGTACATGGGATA20170.1156158161519365No Hit
CTATCGGTCAGTCAGGAGTATTTAG19940.1142974404595743No Hit
GTATCAACGCAGAGTACATGATAAG19310.11068623747614743No Hit
GTCAGGAGTATTTAGCCTTGGAGGA19220.11017035133565788No Hit
TATCAACGCAGAGTACATGATAAGC19090.10942518246606185No Hit
GCCCAGAGCCTGAATCAGTGTGTGT18680.10707503449272056No Hit
CAGTGGTATCAACGCAGAGTACATG17930.10277598332197428No Hit
GGTATCAACGCAGAGTACATGATAA17720.10157224899416534No Hit
TATCAACGCAGAGTACATGGGAAGC17460.10008191125497327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAAGA250.00602805519.0023522
GACAGTT607.7008735E-817.413837
GGACAGT555.920774E-717.2699136
ATTAGGG453.5346617E-516.8914643
CTCCCTA456.775693E-414.77409916
GTTGTAT2750.014.506309512
CAGTTGT2950.014.48833410
AGTTGTA3050.014.32512411
GTTCTAG400.005253069614.2607611
GCCACTC400.00528515714.2476788
TCCTACG602.5739562E-514.2468619
GGACGCC400.005287167614.24686059
CCGCATA400.005287167614.24686059
TGTATCA2850.013.99731714
CAACCTT759.695577E-713.93066312
TGGACAG903.6310666E-813.7247085
GAGTGAT500.001501518213.29783257
TCCTAAG655.4469034E-513.1554752
TATTAGG655.4469034E-513.1554752
TCTACTC957.374001E-813.0019823
Produced by FastQC (version 0.11.5)