##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295316_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1744571
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.75418483971131	31.0	30.0	33.0	25.0	34.0
2	29.557243585958954	31.0	30.0	34.0	23.0	34.0
3	30.110820367872673	31.0	30.0	34.0	25.0	34.0
4	33.15610084083709	35.0	33.0	37.0	25.0	37.0
5	33.926321714622105	35.0	35.0	37.0	28.0	37.0
6	33.944265381001976	37.0	35.0	37.0	28.0	37.0
7	33.93159235135744	37.0	35.0	37.0	28.0	37.0
8	33.860888436182876	37.0	35.0	37.0	28.0	37.0
9	34.764101890951984	39.0	35.0	39.0	25.0	39.0
10	34.699985268584655	38.0	34.0	39.0	25.0	39.0
11	35.14753139883673	39.0	35.0	39.0	27.0	39.0
12	35.17324431049238	39.0	35.0	39.0	27.0	39.0
13	34.94109038841068	39.0	35.0	39.0	27.0	39.0
14	35.15896114288269	39.0	34.0	41.0	22.0	41.0
15	35.7215665054618	39.0	34.0	41.0	27.0	41.0
16	35.73682011222243	39.0	35.0	41.0	27.0	41.0
17	35.752379238219596	39.0	35.0	41.0	27.0	41.0
18	35.67882992437682	39.0	35.0	41.0	26.0	41.0
19	35.73084672392238	39.0	34.0	41.0	26.0	41.0
20	35.770242082437456	39.0	34.0	41.0	26.0	41.0
21	35.786741840830786	39.0	34.0	41.0	26.0	41.0
22	35.58720281375765	39.0	34.0	41.0	25.0	41.0
23	35.26564467711546	39.0	34.0	41.0	24.0	41.0
24	35.59813959993603	39.0	34.0	41.0	25.0	41.0
25	34.5295462322829	38.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6153651398269488
1101	2	0.738575450478546
1101	3	0.44279834396917295
1101	4	0.5008949266210507
1101	5	0.352182393453333
1101	6	0.37732345793821764
1101	7	0.342037177811072
1101	8	0.3476647690800547
1101	9	0.45409560954565364
1101	10	0.7484235355878184
1101	11	0.5817136220682428
1101	12	0.5856429029569128
1101	13	0.642489252057814
1101	14	1.2326771870937137
1101	15	0.8153888704749619
1101	16	0.655142369888793
1101	17	0.8478506070905212
1101	18	0.839728887048885
1101	19	0.9986174535263501
1101	20	0.9488514981135978
1101	21	0.9009803275621238
1101	22	0.8366881372033674
1101	23	-1.4664017431682126
1101	24	0.281192808656904
1101	25	0.5737706751596505
1102	1	0.3107225386249688
1102	2	0.4528368211217142
1102	3	0.31380828112783377
1102	4	0.3667989079523366
1102	5	0.33530879966519933
1102	6	0.23217298626165928
1102	7	0.36924307094371045
1102	8	0.218013953746663
1102	9	0.5572773458017579
1102	10	0.9796598291992495
1102	11	0.7699206302530541
1102	12	0.4733253278202483
1102	13	0.48063059031013466
1102	14	1.2138810122487058
1102	15	0.7913237507383641
1102	16	0.5277801279871497
1102	17	0.8423860753218833
1102	18	0.9487432171508985
1102	19	0.8393500591868133
1102	20	0.9586020386077507
1102	21	1.0431542222332482
1102	22	0.9084457543549931
1102	23	0.8929360210530177
1102	24	0.9729044266726348
1102	25	0.8009558712682647
1103	1	-0.2268514408378266
1103	2	-1.3025804708224626
1103	3	-0.344694087901118
1103	4	0.009665685904344912
1103	5	-0.09789919714237527
1103	6	-0.01536496597556436
1103	7	0.09139616136024387
1103	8	-0.005308756894578437
1103	9	0.7474482643554978
1103	10	0.7932832912975982
1103	11	0.41206124322173565
1103	12	0.4451414709399515
1103	13	0.23021945278280498
1103	14	-0.9086851828603599
1103	15	-0.061454729598409585
1103	16	-0.13997241387131254
1103	17	0.326108064294516
1103	18	0.5654140991715693
1103	19	0.6063264138610762
1103	20	0.5892060707343703
1103	21	0.6455732733851605
1103	22	0.26389789322561086
1103	23	1.1197968188228131
1103	24	0.9253754671906123
1103	25	0.7333219213691535
1104	1	0.028532715869108216
1104	2	-0.10566327417083343
1104	3	-0.2687245948012702
1104	4	-0.11619137220775855
1104	5	-0.10167423826123922
1104	6	-0.22992916734992264
1104	7	-0.14006701100787922
1104	8	-0.09442172378335556
1104	9	-2.2402164601826158
1104	10	-0.6763531305872945
1104	11	-0.5379028879478227
1104	12	-0.15331846863486476
1104	13	-0.07140867021968944
1104	14	-1.7221205124320633
1104	15	-0.5502809757720186
1104	16	-0.269704148742413
1104	17	0.041757144504273924
1104	18	0.11543847158200293
1104	19	0.19420044988626017
1104	20	0.3862559360729705
1104	21	0.40297888394469794
1104	22	0.6046149939204568
1104	23	1.0285087292672301
1104	24	0.6518066565182608
1104	25	0.6848419013807074
1105	1	-0.0742161812781994
1105	2	-0.01101480914837083
1105	3	-1.3824423953927045
1105	4	-0.34215729084206004
1105	5	-0.23428141329324603
1105	6	-0.085503817770892
1105	7	0.053427376022703754
1105	8	-0.013796775135901385
1105	9	0.5080828162645759
1105	10	0.6111684011254894
1105	11	0.11735793934984429
1105	12	0.013171387737976659
1105	13	-0.18337993897109328
1105	14	0.6551325463617843
1105	15	0.30824267402871186
1105	16	-0.23059259683762434
1105	17	0.33736929228034995
1105	18	0.3453220894478122
1105	19	0.3571205318758217
1105	20	-0.3657958025696644
1105	21	-0.031414559798726316
1105	22	0.3336029870593933
1105	23	0.5550653566720811
1105	24	0.462632245463773
1105	25	0.5876881593162722
1106	1	-1.5241515454458678
1106	2	-1.0918339922781755
1106	3	-1.031179737177773
1106	4	-1.4440719925347203
1106	5	-0.8537949234552897
1106	6	-0.6779161483168465
1106	7	-0.5006804250003896
1106	8	-0.5009517275662176
1106	9	-0.1886080740189371
1106	10	-1.620088713820948
1106	11	-1.151192816639636
1106	12	-0.616870445280199
1106	13	-0.8274809158652658
1106	14	-0.10312030559349239
1106	15	-0.7707685328197513
1106	16	-1.3200359162369821
1106	17	-0.5528852646381495
1106	18	-0.5186847412894906
1106	19	-0.3276906209005048
1106	20	-0.13962247902451708
1106	21	-0.2149698279543344
1106	22	-0.1675432761226361
1106	23	-0.5986790998164722
1106	24	-0.6834519270858124
1106	25	-1.4647320740802101
1107	1	0.028801573052568585
1107	2	0.3313525928646648
1107	3	0.13722050347123727
1107	4	0.1380425913783725
1107	5	0.007656444507119886
1107	6	0.1099543994776937
1107	7	0.0900365659328628
1107	8	0.03672918986837459
1107	9	0.47469957771848215
1107	10	-0.0034887026896015527
1107	11	0.0702175243769787
1107	12	-0.17188498228263427
1107	13	0.18171516272200705
1107	14	-0.9568852169535589
1107	15	-0.2897562167850509
1107	16	0.053588104138675874
1107	17	0.08726919598207417
1107	18	0.5315621359715621
1107	19	0.37934836264896177
1107	20	0.4790029062656842
1107	21	0.4710574226307216
1107	22	-0.12689797633174038
1107	23	0.7117554730712925
1107	24	0.5761908628525134
1107	25	0.11962627031820716
1108	1	-0.08119775295579501
1108	2	0.08732996064244247
1108	3	-0.0854999630143567
1108	4	-0.4447917449517931
1108	5	-0.3803370546003677
1108	6	-0.2469660007204766
1108	7	-0.275995645308619
1108	8	-0.43658803714937733
1108	9	0.07853118542477233
1108	10	0.21965617007536764
1108	11	-0.2135544941306975
1108	12	-0.1733053675418077
1108	13	-0.19087866976813928
1108	14	0.08952251185444027
1108	15	-0.10267823456314318
1108	16	-0.04504452664523484
1108	17	0.1461090826681044
1108	18	0.185456174758734
1108	19	0.12497280200256711
1108	20	0.3273050379553126
1108	21	0.14105166243416534
1108	22	0.08585686372726542
1108	23	-0.12509060738941713
1108	24	-0.01688661769726707
1108	25	-0.7889047266960816
1109	1	-0.7079570197986342
1109	2	-0.6677023909041679
1109	3	-0.7142842832285794
1109	4	-0.6787678644595019
1109	5	-0.880613355191656
1109	6	-1.0225142063687755
1109	7	-0.8132380783946402
1109	8	-1.0630133400683306
1109	9	-0.6395043719268969
1109	10	-1.0336667916846594
1109	11	-1.1296852140743212
1109	12	-1.1046478817984635
1109	13	-1.1500946031344625
1109	14	-0.3643854254238761
1109	15	-1.2198870033121878
1109	16	-1.2262739586179023
1109	17	-1.2944077023338494
1109	18	-1.799005663371517
1109	19	-1.799265313446469
1109	20	-1.524862613039403
1109	21	-1.3951518638547213
1109	22	-1.6066497896545116
1109	23	-1.1089150412563384
1109	24	-1.9788757775432018
1109	25	-1.0323489582427428
1110	1	0.0567135767495941
1110	2	0.10692472333048642
1110	3	0.06865278126001684
1110	4	0.22309788002478115
1110	5	-0.015751748162941226
1110	6	0.08879082988723042
1110	7	0.13467135187354984
1110	8	0.04280151464587334
1110	9	0.3123007576088881
1110	10	0.2278867448908244
1110	11	0.25523898713889537
1110	12	0.2182428958951803
1110	13	0.1320010492970809
1110	14	-2.2107707357316855
1110	15	-0.5856514422988894
1110	16	-0.15503701998598984
1110	17	0.01698091369043908
1110	18	-2.600073233244437
1110	19	-0.3128326854634267
1110	20	-1.7386303843939501
1110	21	-0.1713546555921468
1110	22	-1.575969602131707
1110	23	-0.3042699650828098
1110	24	0.20631618196944856
1110	25	0.06905827450526658
1111	1	-0.32892924171504134
1111	2	-1.344602506657516
1111	3	-0.44303392040652767
1111	4	-0.08081394120848984
1111	5	0.020324989086027756
1111	6	-0.06448214539835107
1111	7	-0.10786111065571902
1111	8	-0.042549723105835824
1111	9	-1.615838077289105
1111	10	-0.4052004568433958
1111	11	-0.30627195260296247
1111	12	-0.139434502281226
1111	13	-0.15216668779695652
1111	14	0.8449016015620572
1111	15	0.2180879438657115
1111	16	0.22308877626444712
1111	17	0.03966744369837727
1111	18	0.16766431472769483
1111	19	-0.18305507832223356
1111	20	0.07547696638103218
1111	21	-0.6440386591008149
1111	22	-0.3373473003941072
1111	23	-0.13621117541572403
1111	24	-0.7365627155737826
1111	25	-2.0183989765894665
1112	1	0.243800714079363
1112	2	0.3663886090682844
1112	3	0.10047586502870587
1112	4	0.5156514271152304
1112	5	0.2632710318519216
1112	6	0.1483726625891677
1112	7	0.11991018510948948
1112	8	0.1270401637140779
1112	9	-0.21818052431093093
1112	10	0.5241570146105019
1112	11	0.26906503140682503
1112	12	0.16629576994798612
1112	13	0.26553319324014524
1112	14	0.6894347055408758
1112	15	0.29569360736493167
1112	16	0.27318176191480603
1112	17	-3.665664947915417
1112	18	-0.9969323362644431
1112	19	-1.0709758438545833
1112	20	0.02380752339035297
1112	21	-0.46981456609341876
1112	22	-1.7018627073390178
1112	23	-1.510096549794639
1112	24	-0.27195583061156015
1112	25	0.1536275590973304
1113	1	-0.3577263311124419
1113	2	-0.15216221931220986
1113	3	-0.12673255554738105
1113	4	-0.6699428439297748
1113	5	-0.17810367628754165
1113	6	-0.27482640081135656
1113	7	-0.4173469101239533
1113	8	-0.358828164098675
1113	9	0.05498109421880315
1113	10	0.20769310389612627
1113	11	-0.10102258003667686
1113	12	-0.35547480286667366
1113	13	-0.47293477871772893
1113	14	-1.6616379669058006
1113	15	-1.9679153432453234
1113	16	-0.6542942480126754
1113	17	-0.5111591062950893
1113	18	-0.8866996342146436
1113	19	-0.2925017572485302
1113	20	0.13535796152105917
1113	21	-1.7276263598680899
1113	22	-2.1998148146510914
1113	23	-0.7912762947285614
1113	24	-2.4416424622414894
1113	25	-0.5720849039814411
1114	1	-0.18008144171588825
1114	2	0.052390967502617514
1114	3	-0.18745593334393007
1114	4	0.07925220016549162
1114	5	-0.1914383747663706
1114	6	-0.05281641605192533
1114	7	-0.06757103928377717
1114	8	-0.23996665277596918
1114	9	0.14631466005347704
1114	10	-0.09298658924476655
1114	11	-0.1335578365187189
1114	12	-0.0839281676836734
1114	13	-0.09221276606666606
1114	14	0.7354049999819523
1114	15	0.16295198274395517
1114	16	0.12422369458509053
1114	17	-0.5914733883832071
1114	18	-0.3449992388557135
1114	19	-0.522437051485376
1114	20	-1.703703117007862
1114	21	-0.893877617028437
1114	22	-1.4545818920345752
1114	23	-3.0584832603834187
1114	24	-2.086716366738898
1114	25	-1.686895772672571
1115	1	0.07877239871531216
1115	2	0.1226459962164057
1115	3	0.15093905405768027
1115	4	0.1677845231951025
1115	5	0.0013524676331471142
1115	6	-0.10792094316126821
1115	7	0.005313130529948751
1115	8	0.16539845728510727
1115	9	0.125143688852404
1115	10	0.5025513711336487
1115	11	0.07524502249485465
1115	12	-0.005470502531245813
1115	13	-0.025394675467723005
1115	14	0.5440240015570055
1115	15	0.12263713448209046
1115	16	0.23270971878454105
1115	17	0.10681797396595272
1115	18	0.28145292070902883
1115	19	-0.32525248253681127
1115	20	0.3322818581703686
1115	21	-0.611044266464809
1115	22	0.4100629509365774
1115	23	0.2900157777604093
1115	24	0.34577937129567005
1115	25	0.40304132659625225
1116	1	0.3646486965020479
1116	2	0.48911315305091563
1116	3	0.21807553145934122
1116	4	0.5233073567139073
1116	5	0.24054030407552318
1116	6	0.10039325228360241
1116	7	0.12908025543541157
1116	8	0.15080090010381753
1116	9	0.22320342557283368
1116	10	0.358110738690371
1116	11	0.16680136061516748
1116	12	0.1382186332484423
1116	13	-0.42216613704844264
1116	14	0.5043071692382171
1116	15	0.23589707008362382
1116	16	0.36839169797101334
1116	17	0.37958246043416466
1116	18	0.48282743317079024
1116	19	0.6260042288934002
1116	20	0.37351247410195754
1116	21	-0.06862434015481256
1116	22	-0.11291126639812887
1116	23	-0.8453048543022348
1116	24	-0.5148423394726791
1116	25	-1.08657858856661
1201	1	0.5578783391106761
1201	2	0.7960047228175426
1201	3	0.6247336189446351
1201	4	0.37902725342839005
1201	5	0.5543661299265281
1201	6	0.40135589511858427
1201	7	0.4039022444082292
1201	8	0.39695878219176706
1201	9	0.7146927099796798
1201	10	0.9342418911790347
1201	11	0.5422362580422515
1201	12	0.658867702812465
1201	13	0.6545585019692268
1201	14	1.355230618907484
1201	15	1.0612834825606043
1201	16	0.7705614344196761
1201	17	0.7592597909953369
1201	18	0.763803308065647
1201	19	0.9383059925159927
1201	20	1.0315730621251973
1201	21	1.0344337747167955
1201	22	1.1628109078514726
1201	23	0.8748070383434268
1201	24	1.055040611989277
1201	25	1.2851340060628758
1202	1	0.49593871052920946
1202	2	0.6904605556598398
1202	3	0.5713757475330148
1202	4	0.4939900175170351
1202	5	0.3502785480752948
1202	6	0.32723713579923697
1202	7	0.3104386545454645
1202	8	0.36173439847929956
1202	9	0.8370995441249605
1202	10	0.4265498814080644
1202	11	0.616129208946731
1202	12	0.48603574023649543
1202	13	0.36183506575301294
1202	14	1.087971663338834
1202	15	0.8028265597518143
1202	16	0.5222188612688186
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2210	17	0.519297901246432
2210	18	0.3811483431058278
2210	19	0.5267530662036819
2210	20	0.6147235193891731
2210	21	0.7483720603163775
2210	22	0.9396852768626118
2210	23	1.2188631970898882
2210	24	0.8876443598417012
2210	25	0.7949065843940559
2211	1	0.1455364799247576
2211	2	-0.5748492375784942
2211	3	-0.0326474316604255
2211	4	0.27152244044709306
2211	5	0.22778128394192976
2211	6	0.3041921162406993
2211	7	0.1657249891827064
2211	8	0.2089534191554634
2211	9	0.7300564338520701
2211	10	0.8055049549854161
2211	11	0.4749782822446704
2211	12	0.13023046408859784
2211	13	0.23693955257429167
2211	14	-0.8266588200364069
2211	15	0.20299827904025136
2211	16	0.17213940471670952
2211	17	0.2964675742736631
2211	18	0.2748766028951337
2211	19	0.43182909482622733
2211	20	0.5221568079997851
2211	21	0.40889059539886574
2211	22	-0.20250812405171814
2211	23	0.3889331384534387
2211	24	-0.20687151672359505
2211	25	0.14243254841711206
2212	1	-0.1920154540409733
2212	2	-1.3221344239892616
2212	3	-0.5415338847084463
2212	4	-0.005974756037943507
2212	5	-0.045150779077410164
2212	6	-0.06587806355193493
2212	7	-0.005930325546323445
2212	8	0.07841004280426489
2212	9	-2.3415570579487976
2212	10	-0.12353463432262402
2212	11	-0.00831001101218476
2212	12	-0.028551891231650472
2212	13	0.025243609857909632
2212	14	0.5522817270626419
2212	15	0.09738343603709154
2212	16	0.19506365466155984
2212	17	0.2792482474849294
2212	18	0.16036657458422354
2212	19	-0.6728284167555074
2212	20	0.251697812853088
2212	21	0.2088524492116477
2212	22	-1.5270852988439785
2212	23	-0.40027907384158823
2212	24	0.16150000875622084
2212	25	-0.5372592028145959
2213	1	0.14679386715819476
2213	2	0.3810937027184167
2213	3	0.08990487974261185
2213	4	0.18378024154360872
2213	5	0.18193337639804952
2213	6	0.037290815909607034
2213	7	-0.10342607974107665
2213	8	0.03406589645975089
2213	9	0.4190662553124298
2213	10	0.5079626563987745
2213	11	-0.08605495140551511
2213	12	-0.058970602165096864
2213	13	-0.08276110871661047
2213	14	-1.9315544197373598
2213	15	-0.6457038374466251
2213	16	-0.37132856762121946
2213	17	-2.402872358618218
2213	18	-1.191987761268777
2213	19	-0.6893700400664358
2213	20	-0.05429671918167145
2213	21	-0.3406957640300945
2213	22	0.23171256732212697
2213	23	0.6132815266125036
2213	24	0.40199282963975236
2213	25	-1.1412406644064106
2214	1	-0.022681906441217592
2214	2	0.03937882853210084
2214	3	-0.07739018472396353
2214	4	0.17873751870885712
2214	5	0.030885688740610817
2214	6	0.020633844692966363
2214	7	0.09279834671539788
2214	8	-0.04912601396161875
2214	9	0.35020658574168095
2214	10	0.23306551774928863
2214	11	-0.2511711874288949
2214	12	-0.3550214409675263
2214	13	-0.24229050869643487
2214	14	0.5099607351072422
2214	15	0.21694573553288876
2214	16	0.1032069441050325
2214	17	0.09127597454140357
2214	18	0.09349756108687046
2214	19	-1.2029291689983026
2214	20	-0.1599688098562524
2214	21	0.2058096820016857
2214	22	0.31568516191265417
2214	23	0.9159801083844386
2214	24	0.5534244749353476
2214	25	0.5525058544618062
2215	1	-0.31338449284844216
2215	2	0.14415378856330108
2215	3	-8.302419883001733E-4
2215	4	0.1288876415117528
2215	5	-0.1153182439121423
2215	6	-0.15135761166083483
2215	7	-0.01926625874052945
2215	8	-0.07922523764445089
2215	9	0.2142631735869216
2215	10	0.4506579362012957
2215	11	0.03903034544610762
2215	12	-0.08153482767555431
2215	13	-0.16933598314604836
2215	14	0.5497436313927437
2215	15	-0.0052687249946004044
2215	16	0.09991601696368235
2215	17	0.17883892086437925
2215	18	-0.007435831411314098
2215	19	-0.5551139933641949
2215	20	-1.8185508387299478
2215	21	-2.126680768992685
2215	22	-0.5098513919457943
2215	23	0.13200215059190867
2215	24	0.31119474052388796
2215	25	-0.5460090945151066
2216	1	-0.07478528196198653
2216	2	-0.27046265036699424
2216	3	-0.15972879437956067
2216	4	0.11947418404653831
2216	5	0.08973463556255723
2216	6	0.0024528521468667464
2216	7	-0.07912737240135215
2216	8	-0.2245395339281515
2216	9	0.060375121580662494
2216	10	-1.847940072760835
2216	11	-0.5600655442151634
2216	12	-0.34350124861161646
2216	13	-0.3670233525839919
2216	14	-0.7614441019130638
2216	15	-0.737434783136564
2216	16	-0.3231275740952455
2216	17	-1.9775237534958023E-4
2216	18	-0.2161062606437767
2216	19	-1.62408747725096
2216	20	-1.1188134436082322
2216	21	-1.0892026383002147
2216	22	-0.8678726088543414
2216	23	-4.526538941884564
2216	24	-2.115480341922236
2216	25	-1.458853474410482
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	5.0
5	19.0
6	16.0
7	143.0
8	67.0
9	200.0
10	581.0
11	1310.0
12	2887.0
13	5150.0
14	8273.0
15	12105.0
16	16378.0
17	18342.0
18	18273.0
19	17230.0
20	16588.0
21	15661.0
22	14750.0
23	14357.0
24	14114.0
25	14562.0
26	15632.0
27	17635.0
28	19946.0
29	25134.0
30	31063.0
31	39486.0
32	53571.0
33	73438.0
34	101809.0
35	144812.0
36	212936.0
37	325232.0
38	492865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.65542236683295	18.82196436028875	12.008267440099493	20.514345832778805
2	14.063494320299124	23.524177856562183	38.026936011493554	24.385391811645135
3	20.752543597359075	30.77711235404238	29.334464776659395	19.135879271939153
4	12.974935220794027	20.83112337664944	31.92228674180806	34.271654660748474
5	10.72588344921511	44.31521754712924	31.80227940649416	13.156619597161493
6	28.39380828523944	33.94430300212517	22.537293667314287	15.1245950453211
7	23.716221484328422	38.30284170649153	18.973699538612536	19.007237270567515
8	22.494363493326787	36.85478340447297	23.059562336438095	17.59129076576215
9	27.281109267327142	21.952892147812094	17.24824225715855	33.51775632770221
10	15.921337550587268	29.456165727301443	27.54429807890209	27.078198643209195
11	28.95830060668048	29.39499359608679	19.51129599838555	22.13540979884718
12	25.060080030269326	28.912368001651057	23.481889998509466	22.54566196957015
13	28.717618540907964	22.756345573463715	28.80464944547898	19.72138644014934
14	27.35215003666969	26.336350818000774	22.88320688644781	23.42829225888172
15	31.033587078928722	24.88446127417139	22.910784608292182	21.17116703860771
16	23.583587554960317	26.68596094628351	27.991592769919727	21.738858728836444
17	20.61994980042861	32.53357626654161	25.297293144628195	21.549180788401586
18	21.033074293603587	25.023478700604425	34.26514247185136	19.678304533940626
19	23.189517483664066	32.88129316166699	22.610435965084267	21.318753389584675
20	22.31034836611836	28.943676788338074	28.165790787706317	20.580184057837247
21	23.92844305867745	30.72252308958777	23.991845911576522	21.357187940158255
22	21.84647754896115	29.434653488052103	21.684630555428154	27.034238407558593
23	25.121096712831235	25.58341125527227	23.786553320105234	25.508938711791256
24	20.641514647868107	29.82289778913193	21.345256721095122	28.190330841904842
25	24.044935954157385	30.78280419910275	23.040378548171816	22.13188129856805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11813.0
1	11813.0
2	9331.0
3	6849.0
4	6849.0
5	6849.0
6	9334.5
7	11820.0
8	11820.0
9	11820.0
10	11576.0
11	11332.0
12	11332.0
13	11332.0
14	12384.0
15	13436.0
16	13436.0
17	13436.0
18	17203.5
19	20971.0
20	20971.0
21	20971.0
22	27077.0
23	33183.0
24	33183.0
25	33183.0
26	43712.0
27	54241.0
28	54241.0
29	54241.0
30	61347.5
31	68454.0
32	68454.0
33	68454.0
34	76276.0
35	84098.0
36	84098.0
37	84098.0
38	114032.5
39	143967.0
40	143967.0
41	143967.0
42	277602.0
43	411237.0
44	411237.0
45	411237.0
46	345837.5
47	280438.0
48	280438.0
49	280438.0
50	239471.5
51	198505.0
52	198505.0
53	198505.0
54	165543.5
55	132582.0
56	132582.0
57	132582.0
58	113463.0
59	94344.0
60	94344.0
61	94344.0
62	85587.0
63	76830.0
64	76830.0
65	76830.0
66	59555.0
67	42280.0
68	42280.0
69	42280.0
70	35582.0
71	28884.0
72	28884.0
73	28884.0
74	20551.5
75	12219.0
76	12219.0
77	12219.0
78	8603.5
79	4988.0
80	4988.0
81	4988.0
82	3217.5
83	1447.0
84	1447.0
85	1447.0
86	952.0
87	457.0
88	457.0
89	457.0
90	310.5
91	164.0
92	164.0
93	164.0
94	91.5
95	19.0
96	19.0
97	19.0
98	16.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0772109590266031
2	0.016565677177942315
3	0.027857851586435865
4	0.03267278889767169
5	0.03989519486452543
6	0.013756963746388082
7	0.01530462216785674
8	0.03341795776726771
9	0.03284475094450154
10	0.01765477014119804
11	0.018629221739900524
12	0.013241077605898526
13	0.020807407666411974
14	0.035768105740609006
15	0.03250082685084184
16	0.020520804255028887
17	0.01908778719811346
18	0.02504913815488163
19	0.014731415345090568
20	0.013069115559068676
21	0.00974451598702489
22	0.018973145833560228
23	0.01742548741209157
24	0.018571901057623907
25	0.10965446519516833
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1744571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.76851660494828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94618834721093	15.013434854243691
2	8.151659582383873	3.2229243562448273
3	3.51761970731841	2.0861437078205194
4	2.1510763293561443	1.7009435254155258
5	1.494963844594733	1.4776608792834152
6	1.0862724228899658	1.2884396657638617
7	0.851258755435731	1.1779685989357203
8	0.6439917894454658	1.0184609906482438
9	0.5821367593362199	1.035718217395996
>10	4.718260497799486	18.933177356520403
>50	0.5227980190377772	7.10375503443801
>100	0.2615436324466388	10.018348531958988
>500	0.033785146283812725	4.542980957416429
>1k	0.03116388493420657	12.462739900378788
>5k	0.004077517654942916	5.893830334164176
>10k+	0.003203763871740862	13.023473089371471
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	62065	3.557608145498234	No Hit
GCTTATGTACTCTGCGTTGATACCA	37531	2.1513025265237125	No Hit
CTTATGTACTCTGCGTTGATACCAC	24686	1.4150183626805672	No Hit
GAGTACATAAGCAGTGGTATCAACG	16628	0.9531283048955875	No Hit
TATGTACTCTGCGTTGATACCACTG	16076	0.9214872882788948	No Hit
CCCATGTACTCTGCGTTGATACCAC	14669	0.8408370883156948	No Hit
GCGTTGATACCACTGCTTATGTACT	12725	0.7294056819699514	No Hit
CATAAGCAGTGGTATCAACGCAGAG	10604	0.6078285148612467	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	10462	0.599688977977967	No Hit
CATGTACTCTGCGTTGATACCACTG	10390	0.5955618888540506	No Hit
ACTCTGCGTTGATACCACTGCTTAT	10360	0.5938422683857522	No Hit
GTACATGATAAGCAGTGGTATCAAC	9515	0.5454062918620108	No Hit
ATCATGTACTCTGCGTTGATACCAC	9319	0.5341714381357938	No Hit
CTGCTTATGTACTCTGCGTTGATAC	9227	0.5288979353663451	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	9073	0.520070550295746	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8146	0.46693427782532215	No Hit
GTATCAACGCAGAGTACATAAGCAG	8101	0.46435484712287434	No Hit
GTGGTATCAACGCAGAGTACATGGG	7297	0.4182690185724743	No Hit
TATCAACGCAGAGTACATAAGCAGT	6916	0.39642983862508313	No Hit
GGTATCAACGCAGAGTACATAAGCA	6459	0.3702342868246692	No Hit
GTACTGGTTCACTATCGGTCAGTCA	6256	0.35859818832251594	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5989	0.3432935661546592	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	5642	0.3234032894046731	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5274	0.3023092783268781	No Hit
GTGGTATCAACGCAGAGTACATAAG	5152	0.2953161550891308	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4738	0.27158539262661135	No Hit
ATACAGGGTGACAGCCCCGTACACA	4462	0.25576488431826505	No Hit
GCAGTGGTATCAACGCAGAGTACAT	4456	0.25542096022460536	No Hit
GGATACCACGTGTCCCGCCCTACTC	4401	0.2522683226993914	No Hit
GTACATGGGATAAGCAGTGGTATCA	4242	0.24315433421740934	No Hit
ACATAAGCAGTGGTATCAACGCAGA	4028	0.2308877082102133	No Hit
GTACTCTGCGTTGATACCACTGCTT	3988	0.2285948809191486	No Hit
GCTTATCCCATGTACTCTGCGTTGA	3723	0.21340490011584506	No Hit
GAGTACATGATAAGCAGTGGTATCA	3695	0.2117999210120998	No Hit
CCACTGCTTATGTACTCTGCGTTGA	3626	0.20784479393501326	No Hit
GATACCACTGCTTATGTACTCTGCG	3547	0.2033164600351605	No Hit
GTTGATACCACTGCTTATGTACTCT	3233	0.18531776580030276	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	3215	0.18428599351932365	No Hit
GCTTATCATGTACTCTGCGTTGATA	3191	0.18291029714468485	No Hit
ATACCACTGCTTATGTACTCTGCGT	3084	0.17677698414108683	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3021	0.17316578115765996	No Hit
GTTAATGATAGTGTGTCGAAACACA	2890	0.16565677177942315	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	2737	0.15688670739110072	No Hit
GGTATCAACGCAGAGTACATGGGGG	2703	0.15493780419369574	No Hit
GATAAGCAGTGGTATCAACGCAGAG	2695	0.15447923873548283	No Hit
CCCATATTCAGACAGGATACCACGT	2642	0.1514412425748221	No Hit
GCGTTGATACCACTGCTTATCATGT	2630	0.1507533943875027	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	2613	0.14977894278880022	No Hit
GGATAAGCAGTGGTATCAACGCAGA	2574	0.14754343618001217	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2571	0.1473714741331823	No Hit
ATCATTAACTGAATCCATAGGTTAA	2552	0.1462823811699266	No Hit
CAGTGGTATCAACGCAGAGTACATA	2512	0.1439895538788619	No Hit
TGATACCACTGCTTATGTACTCTGC	2396	0.13734035473477432	No Hit
TACCACTGCTTATGTACTCTGCGTT	2385	0.13670982722973155	No Hit
GTATCAACGCAGAGTACATGGGAAG	2376	0.13619394108924202	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	2346	0.1344743206209435	No Hit
GTATCAACGCAGAGTACATGGGGGT	2328	0.13344254833996438	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	2278	0.13057651422613353	No Hit
GGTATCAACGCAGAGTACATGGGAA	2257	0.12937277989832457	No Hit
ACGCAGAGTACATGATAAGCAGTGG	2216	0.12702263192498328	No Hit
GAGTACATGGGATAAGCAGTGGTAT	2215	0.12696531124270666	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	2202	0.12622014237311063	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2191	0.12558961486806786	No Hit
TATTCAGACAGGATACCACGTGTCC	2188	0.12541765282123798	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	2139	0.12260893938968377	No Hit
GGTATCAACGCAGAGTACATGGGAT	2132	0.12220769461374745	No Hit
GCGTTGATACCACTGCTTCCCATGT	2100	0.1203734327808957	No Hit
CCCCATGTACTCTGCGTTGATACCA	2083	0.1193989811821932	No Hit
GGGATAAGCAGTGGTATCAACGCAG	2083	0.1193989811821932	No Hit
GCAGAGTACATAAGCAGTGGTATCA	2078	0.11911237777081013	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	2026	0.11613170229242605	No Hit
GTATCAACGCAGAGTACATGGGATA	2017	0.1156158161519365	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	1994	0.1142974404595743	No Hit
GTATCAACGCAGAGTACATGATAAG	1931	0.11068623747614743	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	1922	0.11017035133565788	No Hit
TATCAACGCAGAGTACATGATAAGC	1909	0.10942518246606185	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	1868	0.10707503449272056	No Hit
CAGTGGTATCAACGCAGAGTACATG	1793	0.10277598332197428	No Hit
GGTATCAACGCAGAGTACATGATAA	1772	0.10157224899416534	No Hit
TATCAACGCAGAGTACATGGGAAGC	1746	0.10008191125497327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.7320682276617005E-5	0.0
2	0.0	0.0	0.0	5.7320682276617005E-5	0.0
3	0.0	0.0	0.0	5.7320682276617005E-5	0.0
4	0.0	0.0	0.0	5.7320682276617005E-5	0.0
5	5.7320682276617005E-5	0.0	0.0	5.7320682276617005E-5	0.0
6	1.1464136455323401E-4	0.0	0.0	5.7320682276617005E-5	0.0
7	1.1464136455323401E-4	0.0	0.0	5.7320682276617005E-5	0.0
8	1.1464136455323401E-4	0.0	0.0	5.7320682276617005E-5	0.0
9	1.1464136455323401E-4	0.0	0.0	1.1464136455323401E-4	0.0
10	1.1464136455323401E-4	0.0	0.0	1.71962046829851E-4	0.0
11	1.1464136455323401E-4	0.0	0.0	1.71962046829851E-4	0.0
12	1.1464136455323401E-4	0.0	0.0	1.71962046829851E-4	0.0
13	1.1464136455323401E-4	0.0	0.0	1.71962046829851E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAAGA	25	0.006028055	19.002352	2
GACAGTT	60	7.7008735E-8	17.41383	7
GGACAGT	55	5.920774E-7	17.269913	6
ATTAGGG	45	3.5346617E-5	16.891464	3
CTCCCTA	45	6.775693E-4	14.774099	16
GTTGTAT	275	0.0	14.5063095	12
CAGTTGT	295	0.0	14.488334	10
AGTTGTA	305	0.0	14.325124	11
GTTCTAG	40	0.0052530696	14.260761	1
GCCACTC	40	0.005285157	14.247678	8
TCCTACG	60	2.5739562E-5	14.246861	9
GGACGCC	40	0.0052871676	14.2468605	9
CCGCATA	40	0.0052871676	14.2468605	9
TGTATCA	285	0.0	13.997317	14
CAACCTT	75	9.695577E-7	13.930663	12
TGGACAG	90	3.6310666E-8	13.724708	5
GAGTGAT	50	0.0015015182	13.2978325	7
TCCTAAG	65	5.4469034E-5	13.155475	2
TATTAGG	65	5.4469034E-5	13.155475	2
TCTACTC	95	7.374001E-8	13.001982	3
>>END_MODULE