FastQCFastQC Report
Wed 25 May 2016
SRR1295316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744571
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA661073.7892983432603202No Hit
GCTTATGTACTCTGCGTTGATACCA407202.3340981823038445No Hit
CTTATGTACTCTGCGTTGATACCAC282501.6193092743144302No Hit
GTATCAACGCAGAGTACTTTTTTTT246071.4104900287807147No Hit
GAGTACATAAGCAGTGGTATCAACG180711.0358420494207459No Hit
CCCATGTACTCTGCGTTGATACCAC174571.000647150502903No Hit
GGTATCAACGCAGAGTACTTTTTTT169280.9703245095785726No Hit
TATCAACGCAGAGTACTTTTTTTTT156270.8957503019366939No Hit
GCGTTGATACCACTGCTTATGTACT141330.8101132026154281No Hit
TATGTACTCTGCGTTGATACCACTG140740.8067312823611077No Hit
CATAAGCAGTGGTATCAACGCAGAG130130.745914038465617No Hit
ACTCTGCGTTGATACCACTGCTTAT117900.6758108440413145No Hit
CATGTACTCTGCGTTGATACCACTG113940.6531118538597741No Hit
GTACTTTTTTTTTTTTTTTTTTTTT113450.6503031404282199No Hit
ACGCAGAGTACATAAGCAGTGGTAT108720.62319045771138No Hit
CTGCTTATGTACTCTGCGTTGATAC106490.6104079455636945No Hit
ATCATGTACTCTGCGTTGATACCAC105180.6028989361854576No Hit
GTACATGATAAGCAGTGGTATCAAC96400.5525713771465879No Hit
GTATCAACGCAGAGTACATAAGCAG87970.5042500419873998No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83700.4797741106552843No Hit
TATCAACGCAGAGTACATAAGCAGT76400.4379300125933539No Hit
GTGGTATCAACGCAGAGTACATGGG72470.4154029844586434No Hit
GGTATCAACGCAGAGTACATAAGCA70620.40479865823746924No Hit
GTACTGGTTCACTATCGGTCAGTCA66900.38347536443056773No Hit
AAGCAGTGGTATCAACGCAGAGTAC63190.3622093913059428No Hit
GTACATGGGAAGCAGTGGTATCAAC59650.3419178697800204No Hit
GTGGTATCAACGCAGAGTACATAAG53450.30637904676851785No Hit
ACATAAGCAGTGGTATCAACGCAGA50540.28969872822602233No Hit
ATACAGGGTGACAGCCCCGTACACA49200.28201775680095564No Hit
GCAGTGGTATCAACGCAGAGTACAT48880.2801834949681039No Hit
GGATACCACGTGTCCCGCCCTACTC47640.2730757303658034No Hit
GAGTACTTTTTTTTTTTTTTTTTTT44010.2522683226993914No Hit
GTACTCTGCGTTGATACCACTGCTT41930.2403456207858551No Hit
CCACTGCTTATGTACTCTGCGTTGA41900.24017365873902524No Hit
GCTTATCCCATGTACTCTGCGTTGA41370.23713566257836452No Hit
GTACATGGGATAAGCAGTGGTATCA41130.23575996620372572No Hit
GATACCACTGCTTATGTACTCTGCG39840.22836559819004215No Hit
GAGTACATGATAAGCAGTGGTATCA38280.21942357175488988No Hit
ACCCTGTATCGCGCGCCTTTCCAGA35830.20538000459711872No Hit
ATACCACTGCTTATGTACTCTGCGT34310.19666726089107292No Hit
GCTTATCATGTACTCTGCGTTGATA33260.19064858925202816No Hit
GTTGATACCACTGCTTATGTACTCT31800.18227976963964207No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31610.18119067667638633No Hit
CCCATATTCAGACAGGATACCACGT30270.17350970525131965No Hit
GATAAGCAGTGGTATCAACGCAGAG29880.1712741986425316No Hit
GCGTTGATACCACTGCTTATCATGT29790.17075831250204207No Hit
GTTAATGATAGTGTGTCGAAACACA29690.17018510567927586No Hit
CAGTGGTATCAACGCAGAGTACATA29680.17012778499699927No Hit
GGATAAGCAGTGGTATCAACGCAGA29430.16869476794008384No Hit
ATCATTAACTGAATCCATAGGTTAA28290.16216021016054952No Hit
GGTATCAACGCAGAGTACATGGGGG28260.16198824811371965No Hit
GGTTAATGAGGCGAACCGGGGGAAC27430.15723063148476044No Hit
TACCACTGCTTATGTACTCTGCGTT26980.15465120078231268No Hit
GTATTTAGCCTTGGAGGATGGTCCC26790.15356210781905694No Hit
ACGCAGAGTACATGATAAGCAGTGG26580.15235837349124798No Hit
GAGTACATGGGAAGCAGTGGTATCA26130.14977894278880022No Hit
GCAGAGTACATAAGCAGTGGTATCA25790.14783003959139526No Hit
CCCCATGTACTCTGCGTTGATACCA25740.14754343618001217No Hit
GCCTTGGAGGATGGTCCCCCCATAT25280.1449066847952878No Hit
TCTAAGTACCCCGAGGAAAAGAAAT24850.14244189545739325No Hit
TGATACCACTGCTTATGTACTCTGC24840.14238457477511662No Hit
GGGATAAGCAGTGGTATCAACGCAG24740.14181136795235047No Hit
GTATCAACGCAGAGTACATGGGGGT24370.13969050270811562No Hit
CTATCGGTCAGTCAGGAGTATTTAG24160.13848676838030669No Hit
GTATCAACGCAGAGTACATGGGAAG24110.1382001649689236No Hit
ACTTAGATGTTTCAGTTCCCCCGGT23910.13705375132339123No Hit
GTGGTATCAACGCAGAGTACTTTTT23680.13573537563102905No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG23470.1345316413032201No Hit
ATCAACGCAGAGTACTTTTTTTTTT23420.13424503789183703No Hit
TATTCAGACAGGATACCACGTGTCC23380.13401575516273054No Hit
GCGTTGATACCACTGCTTCCCATGT23330.13372915175134745No Hit
CACACACACTGATTCAGGCTCTGGG23260.13332790697541114No Hit
GGTATCAACGCAGAGTACATGGGAT23260.13332790697541114No Hit
GGTATCAACGCAGAGTACATGGGAA23240.13321326561085792No Hit
GTTCACTATCGGTCAGTCAGGAGTA23020.13195221060077233No Hit
GTCAGGAGTATTTAGCCTTGGAGGA22930.13143632446028278No Hit
GAGTACATGGGATAAGCAGTGGTAT22380.12828368693506886No Hit
TATCAACGCAGAGTACATGATAAGC21780.12484444599847183No Hit
GTATCAACGCAGAGTACATGGGATA21570.12364071167066286No Hit
CAGTGGTATCAACGCAGAGTACATG21440.12289554280106685No Hit
GCCCAGAGCCTGAATCAGTGTGTGT21010.12043075346317232No Hit
ATGATAAGCAGTGGTATCAACGCAG19660.11269246135582901No Hit
ATCAACGCAGAGTACATAAGCAGTG19620.11246317862672256No Hit
GTATCAACGCAGAGTACATGATAAG19350.11091552020525389No Hit
ACGCAGAGTACATGGGAAGCAGTGG19180.10994106860655142No Hit
GCGTTGATACCACTGCTTATCCCAT18850.10804948609142305No Hit
CATCTAAGTACCCCGAGGAAAAGAA18570.10644450698767778No Hit
ACTCTGCGTTGATACCACTGCTTCC18360.10524077265986881No Hit
TATCAACGCAGAGTACATGGGAAGC18220.10443828310799617No Hit
GCCTGAATCAGTGTGTGTGTTAGTG17990.103119907415634No Hit
GTACACAAGCAGTGGTATCAACGCA17740.10168689035871856No Hit
CGGTACTGGTTCACTATCGGTCAGT17660.10122832490050562No Hit
CCCCAGTAGCGGCGAGCGAACGGGG17630.10105636285367577No Hit
GGTATCAACGCAGAGTACATGATAA17580.1007697594422927No Hit
TCATTAACCTATGGATTCAGTTAAT17530.10048315603090961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATGT307.6057884E-419.0433431
TCATATA453.507755E-516.906522
TCGGTAC350.002155267416.3027153
CCGTACG350.002177682716.2774859
CCCTCAG1200.015.0759791
TTGACAG456.709622E-414.7932055
ACGCACA1300.014.60799811
GACTAGA602.5140092E-514.2825061
TAAGGAT400.00524302414.2648764
TACGTGT400.00529717914.24279912
ACTGTAC551.9430924E-413.8326083
GGCCGTG551.9687912E-413.8111997
CTCTTAA707.0947863E-613.6023861
GTTGACA707.1858885E-613.5855973
TAGAAGT852.6655653E-713.4257674
CGCACAG1351.8189894E-1213.36361412
CTTCTAG500.001474143413.33033851
ATGCAAC500.001504748113.2940436
CGTCGCA500.001505398313.2932817
CGCCCTT500.001505398313.2932816
Produced by FastQC (version 0.11.5)