FastQCFastQC Report
Wed 25 May 2016
SRR1295315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4366158
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG60390.13831382190016944No Hit
GTATCAACGCAGAGTACTTTTTTTT50380.11538748712254573No Hit
ATTCCATTCCATTCCATTCCATTCC45570.10437093664498627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG1457.2759576E-1212.4587651
CGCAGAT1502.4543624E-810.1318798
GTGGTAT18400.09.8180481
GGTATCA34200.09.8138181
TGGTATC17750.09.7422382
TCTAGAC3200.09.5014673
ACACCGT1856.6029315E-88.7281776
TAGACAG7100.08.6985265
CGTCAGA2201.6043487E-98.6375982
TAGTACG1000.0029025218.5513214
CTAGGAC4350.08.3001323
TATTCCG2752.1827873E-118.292195
CTAGTAC3100.08.2754723
CCTACAC4250.08.2718663
GTACTAG3450.08.2678311
GTATCAA59600.08.215811
TTTAGAC4550.08.14411453
TAGGACT6100.08.0996114
GTCTAGA3550.08.0349331
GTATAGA6200.07.9744561
Produced by FastQC (version 0.11.5)