##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295315_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4366158
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.071877838594023	31.0	30.0	33.0	26.0	34.0
2	29.91173498531203	31.0	30.0	34.0	25.0	34.0
3	30.370927025545114	31.0	30.0	34.0	26.0	34.0
4	33.63486410707079	35.0	35.0	37.0	28.0	37.0
5	33.9263975330256	35.0	35.0	37.0	28.0	37.0
6	33.96128793323558	36.0	35.0	37.0	28.0	37.0
7	33.939606170917315	36.0	35.0	37.0	28.0	37.0
8	33.90614311254883	36.0	35.0	37.0	28.0	37.0
9	34.9500549453318	38.0	35.0	39.0	27.0	39.0
10	34.855010285931016	38.0	34.0	39.0	27.0	39.0
11	35.24877317769994	38.0	35.0	39.0	27.0	39.0
12	35.25175062377495	39.0	35.0	39.0	27.0	39.0
13	35.153779592951054	38.0	35.0	39.0	27.0	39.0
14	35.34958583725096	39.0	34.0	41.0	25.0	41.0
15	35.96016154248197	39.0	35.0	41.0	27.0	41.0
16	36.0553431644022	39.0	35.0	41.0	27.0	41.0
17	35.85933422473488	39.0	35.0	41.0	27.0	41.0
18	35.829998593729314	39.0	34.0	41.0	27.0	41.0
19	35.88697225340906	39.0	34.0	41.0	27.0	41.0
20	35.873306463027674	39.0	34.0	41.0	27.0	41.0
21	35.85392512135383	39.0	34.0	41.0	27.0	41.0
22	35.59292036614342	39.0	34.0	41.0	25.0	41.0
23	35.26593861239103	39.0	34.0	40.0	24.0	41.0
24	35.6034252539647	39.0	34.0	41.0	25.0	41.0
25	34.2470169425843	38.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3890101509251416
1101	2	0.5453409905742959
1101	3	0.40086689468234127
1101	4	0.28148044640281
1101	5	0.23595742908127448
1101	6	0.17717547912683074
1101	7	0.15964426934911558
1101	8	0.1660095588509023
1101	9	0.11384542499843775
1101	10	0.4206257539353544
1101	11	0.3765475030909755
1101	12	0.3435324020642412
1101	13	0.41618451612431784
1101	14	0.898062972752804
1101	15	0.5375069451689214
1101	16	0.5251358601313783
1101	17	0.7735167870914594
1101	18	0.7643495658241832
1101	19	0.749711426813235
1101	20	0.8584561763144336
1101	21	0.79177836150145
1101	22	0.625622603219739
1101	23	-1.6252580190715307
1101	24	-0.03552002541228205
1101	25	0.4879347304601822
1102	1	0.17267156055118704
1102	2	0.15914000734045786
1102	3	0.22443181433089165
1102	4	0.2187858238419551
1102	5	0.06599987930110984
1102	6	0.09407661375820453
1102	7	-0.07016691536767183
1102	8	0.027173529257666473
1102	9	0.2837485702867042
1102	10	0.7387126076389094
1102	11	0.4502645481586569
1102	12	0.411048150104385
1102	13	0.34520918123727284
1102	14	0.7718794628018415
1102	15	0.5803423660114433
1102	16	0.3895850875525042
1102	17	0.6517189855627166
1102	18	0.6373946124038667
1102	19	0.6288418071214608
1102	20	0.7324290198470464
1102	21	0.7304955162610867
1102	22	0.5681357108421281
1102	23	0.5357847785174741
1102	24	0.6507666941388095
1102	25	0.3875912259772889
1103	1	-0.37291476656088207
1103	2	-1.6024839773968012
1103	3	-0.48823289187575725
1103	4	-0.2278054641820475
1103	5	-0.2408838831437876
1103	6	-0.17533818769396703
1103	7	-0.11092587557311617
1103	8	0.05310823562047062
1103	9	0.6017085059421348
1103	10	0.7013463216727231
1103	11	0.4964277939573378
1103	12	0.22664147526886325
1103	13	0.005716253910598823
1103	14	-1.1694543495574976
1103	15	-0.36233327303762053
1103	16	-0.19448252232344743
1103	17	0.3280071306906578
1103	18	0.4245955233910692
1103	19	0.41882623063109037
1103	20	0.5048262026882071
1103	21	0.5740909648344754
1103	22	0.1099229223417666
1103	23	0.9453345613255877
1103	24	0.7442664317612966
1103	25	0.5985554992337043
1104	1	-0.03951905955603152
1104	2	0.024417502851537876
1104	3	-0.4542833157455881
1104	4	-0.3172757062671394
1104	5	-0.2473102346368421
1104	6	-0.23891184169533375
1104	7	-0.24609234507352795
1104	8	-0.2300942957520391
1104	9	-2.16300541569651
1104	10	-0.7616588886430122
1104	11	-0.5812897290859809
1104	12	-0.16817595823683007
1104	13	-0.046568123878344636
1104	14	-1.8454039910952176
1104	15	-0.6527741841843806
1104	16	-0.34103222270885425
1104	17	0.020184844006877256
1104	18	0.15088229450389434
1104	19	0.1959479023620645
1104	20	0.3948980891210141
1104	21	0.445998281237479
1104	22	0.4976125095768893
1104	23	0.9098591832906138
1104	24	0.5629576591779255
1104	25	0.35655575871390965
1105	1	-0.2872819281981407
1105	2	-0.23195341835574723
1105	3	-1.4691954769956617
1105	4	-0.5849558817968656
1105	5	-0.56350200601441
1105	6	-0.32916504567807436
1105	7	-0.19980807840912718
1105	8	-0.39503402050318925
1105	9	0.04126860276782196
1105	10	0.2852747053668878
1105	11	-0.3621706524605415
1105	12	-0.3345050893291486
1105	13	-0.38403494265799765
1105	14	0.38140539839404397
1105	15	0.0644737978782004
1105	16	-0.3349327440517058
1105	17	0.11614292537846893
1105	18	-0.010949198088212597
1105	19	-0.07719278999712031
1105	20	-0.7008559789355573
1105	21	-0.14035882821382728
1105	22	-0.01787344356912257
1105	23	0.14557385716117466
1105	24	0.09148319652862114
1105	25	0.25634354549155347
1106	1	-1.7341289620066043
1106	2	-1.2566977143184275
1106	3	-1.2537427979634614
1106	4	-1.5372684855782524
1106	5	-1.0120339388672548
1106	6	-0.9413393529376677
1106	7	-0.7498434756749504
1106	8	-0.9157833498286436
1106	9	-0.5520481453592652
1106	10	-2.054111433033448
1106	11	-1.5260391092259624
1106	12	-1.1713217508964178
1106	13	-1.2739193745547226
1106	14	-0.47451400006680444
1106	15	-1.3282003256203154
1106	16	-1.6615063821383984
1106	17	-1.0074234235916535
1106	18	-1.0126710011852254
1106	19	-0.8142013603585667
1106	20	-0.6755249239771146
1106	21	-0.6243966287724376
1106	22	-0.537945966980999
1106	23	-0.9368558480173874
1106	24	-1.1099925744038615
1106	25	-1.8509786637662131
1107	1	-0.005315120001046836
1107	2	0.04589131729763807
1107	3	-0.04604866586552703
1107	4	0.1271997504845288
1107	5	-0.14596360137463193
1107	6	-0.03389422876912107
1107	7	-0.17506388557643504
1107	8	-0.18160429204019124
1107	9	0.29336030972894633
1107	10	-0.12643421414167477
1107	11	-0.13894296377258541
1107	12	-0.1821421615479366
1107	13	-0.05794494608289824
1107	14	-1.3267680945901432
1107	15	-0.6493416038626592
1107	16	-0.3374054655236449
1107	17	-0.14905817753611217
1107	18	0.13425105410486537
1107	19	0.05377751076584758
1107	20	0.1316677579081329
1107	21	0.227841996355032
1107	22	-0.3483556417336189
1107	23	0.47323655362013284
1107	24	0.27908024897380557
1107	25	0.0625409245475197
1108	1	-0.1317324377644553
1108	2	0.24111969464367888
1108	3	-0.14362173543577583
1108	4	-0.42605353681184965
1108	5	-0.3417439035708085
1108	6	-0.37464280261962557
1108	7	-0.28665362724771626
1108	8	-0.3444831266170496
1108	9	0.1834320391289026
1108	10	0.275669481242403
1108	11	-0.01586153285756353
1108	12	-0.047388006807480565
1108	13	-0.130888193856471
1108	14	0.05182826992427181
1108	15	-0.1311751226163338
1108	16	-0.13736805553611475
1108	17	0.18496752397555127
1108	18	0.14046538442570267
1108	19	0.1378662991201125
1108	20	0.29391974138142274
1108	21	0.21891027680920416
1108	22	0.15191277492692024
1108	23	0.01347443862948694
1108	24	0.060400294392934484
1108	25	-0.7346241848575588
1109	1	-0.7480265263413699
1109	2	-0.6508581395370783
1109	3	-0.7933390686807122
1109	4	-0.5572537004667595
1109	5	-0.9196733679542746
1109	6	-1.1068633668714014
1109	7	-0.7714420513669324
1109	8	-1.2138134892705779
1109	9	-0.8504931620266305
1109	10	-1.0756873017346962
1109	11	-1.215685250519691
1109	12	-1.1405885857061975
1109	13	-1.1349544645003533
1109	14	-0.6637266454152808
1109	15	-1.1916047647433388
1109	16	-1.2495527134114184
1109	17	-1.297763436855881
1109	18	-1.9663310706483657
1109	19	-1.929133616697051
1109	20	-1.6415869847395541
1109	21	-1.476258195717314
1109	22	-1.5489187595254492
1109	23	-0.9616261118738905
1109	24	-1.81242077696227
1109	25	-1.117455401770549
1110	1	0.033661485927449775
1110	2	0.18178556400416213
1110	3	0.031461709532703
1110	4	0.31265355016778784
1110	5	0.15858146529586037
1110	6	0.19341562993699313
1110	7	0.12452265723177902
1110	8	0.06848341312731065
1110	9	0.35737015458030896
1110	10	0.3395799365541663
1110	11	0.31463418039380286
1110	12	0.23220449524001907
1110	13	0.3418348277866272
1110	14	-2.4193878102844053
1110	15	-0.6647678874310614
1110	16	-0.0819681274901356
1110	17	-0.22405259572679626
1110	18	-2.7558254432378746
1110	19	-0.457324753532788
1110	20	-1.828878733750031
1110	21	-0.24032834489889154
1110	22	-1.613419754551515
1110	23	-0.49498441517329184
1110	24	0.13581256676877018
1110	25	0.08968241866310933
1111	1	-0.07017757799768276
1111	2	-1.1937130571426664
1111	3	-0.26195991419702125
1111	4	0.17772876609782884
1111	5	0.0982298110245381
1111	6	0.24069950401173656
1111	7	0.10070503183185053
1111	8	0.1152200323450927
1111	9	-1.4907230918943952
1111	10	-0.11798970284846888
1111	11	0.0568586396842079
1111	12	0.16362458584006845
1111	13	0.2526414568423476
1111	14	1.1159886145815463
1111	15	0.5248046532383981
1111	16	0.5831658336995744
1111	17	0.22015135580798528
1111	18	0.32577299907748625
1111	19	0.15089860110757058
1111	20	0.5313142646884543
1111	21	-0.42902851381625595
1111	22	-0.08577897060347794
1111	23	0.2290510718761567
1111	24	-0.2546739937431397
1111	25	-1.6946445270473802
1112	1	0.3521130572002029
1112	2	0.5562750174013544
1112	3	0.19093451472093292
1112	4	0.5233716715213177
1112	5	0.3723516154564024
1112	6	0.3506777954904763
1112	7	0.3884048710387873
1112	8	0.25320921405044317
1112	9	-0.09309002845491676
1112	10	0.7433777395908976
1112	11	0.3350619838175817
1112	12	0.41492627680386107
1112	13	0.33685419691774143
1112	14	0.7807989750823907
1112	15	0.3945194358500075
1112	16	0.44746865477745956
1112	17	-3.611322151936257
1112	18	-0.7781533280700828
1112	19	-1.0364092967616685
1112	20	0.07451808603965304
1112	21	-0.2852508055931082
1112	22	-1.4230829585198634
1112	23	-1.1982393731132035
1112	24	-0.022994118727510227
1112	25	0.25893914332894497
1113	1	-0.0798767750083087
1113	2	0.07888943215482058
1113	3	0.06646375901393142
1113	4	-0.25357294862412516
1113	5	0.09321885825944776
1113	6	0.16357250374549892
1113	7	-0.07990231224106736
1113	8	0.11935849445444546
1113	9	0.5642352241314583
1113	10	0.644289927363765
1113	11	0.171871090597719
1113	12	0.20655985575120894
1113	13	-0.14727855365062226
1113	14	-1.0499708609582257
1113	15	-1.4855913349327494
1113	16	-0.19464429221088864
1113	17	0.014998760638313513
1113	18	-0.3789544752375278
1113	19	0.17071512119925814
1113	20	0.5914996406970303
1113	21	-1.208112599381991
1113	22	-1.5354154374407543
1113	23	-0.3017068517225425
1113	24	-1.9862459481992687
1113	25	-0.31825544490178004
1114	1	0.256520040534884
1114	2	0.3108343202209767
1114	3	0.10292680008052812
1114	4	0.5165874987261603
1114	5	0.16632972040967076
1114	6	0.3161325327075559
1114	7	0.3885531969850291
1114	8	0.35200916912601343
1114	9	0.7186723151734427
1114	10	0.5269659560150188
1114	11	0.371242983137023
1114	12	0.42562238788127615
1114	13	0.3806728485960704
1114	14	1.2975464695884895
1114	15	0.6237439828831697
1114	16	0.5409523604092996
1114	17	-0.03777039394516635
1114	18	0.1579301428077926
1114	19	-0.09952146799907524
1114	20	-1.296763281784628
1114	21	-0.5158832383700087
1114	22	-1.303762864055571
1114	23	-2.5908212539169497
1114	24	-1.7504463648066704
1114	25	-1.0454405732458838
1115	1	0.4914477254422387
1115	2	0.5225646666762884
1115	3	0.383523751728962
1115	4	0.696123948020805
1115	5	0.5466836228363192
1115	6	0.4243627360778959
1115	7	0.5372259652637865
1115	8	0.556031614222384
1115	9	0.8196883022004045
1115	10	0.9767526673532032
1115	11	0.640391462949772
1115	12	0.5491094664430207
1115	13	0.5560700418991331
1115	14	1.2912564204375414
1115	15	0.7803836558572925
1115	16	0.7188950159661047
1115	17	0.6760404583113697
1115	18	0.9283179114686675
1115	19	0.3941136212351921
1115	20	0.9029950078003992
1115	21	0.02084324506978419
1115	22	0.983472003383028
1115	23	1.0289436627618542
1115	24	1.1236410291556425
1115	25	1.3058303170216021
1116	1	0.6710167190137888
1116	2	0.7573693792169855
1116	3	0.5803284146429206
1116	4	0.9559710441158416
1116	5	0.7644564569817973
1116	6	0.6602720719561148
1116	7	0.6957279447101072
1116	8	0.7435173219898061
1116	9	0.8837065077154307
1116	10	0.8625520121731611
1116	11	0.789748591411076
1116	12	0.8235760281080786
1116	13	0.2813826055503341
1116	14	1.3391465254195154
1116	15	0.9859057728696357
1116	16	0.8985783116955801
1116	17	1.1578582571370717
1116	18	1.0583208087418612
1116	19	1.2650470626762669
1116	20	1.097599189049383
1116	21	0.6538243178249061
1116	22	0.6610881491385427
1116	23	-0.2258131403408541
1116	24	0.2050831285879866
1116	25	-0.24833287532716497
1201	1	0.39902637207669756
1201	2	0.6255372128969654
1201	3	0.5692451352557875
1201	4	0.40377788448089547
1201	5	0.4122061397925023
1201	6	0.2966813142959239
1201	7	0.3698144199854738
1201	8	0.3279785880847328
1201	9	0.5617530081567281
1201	10	0.9077803425489392
1201	11	0.5593802909044783
1201	12	0.5639790008438936
1201	13	0.49308051629719074
1201	14	1.12358816406703
1201	15	0.7482667727323715
1201	16	0.503187975027366
1201	17	0.5565438569707908
1201	18	0.49131978608637183
1201	19	0.8588060534326019
1201	20	0.8773955187486635
1201	21	0.805577305201453
1201	22	0.8294631809492969
1201	23	0.4953063044946404
1201	24	0.7765275961272025
1201	25	0.9553937205587815
1202	1	0.34138294144409187
1202	2	0.6439514581918644
1202	3	0.4465712075599342
1202	4	0.37948874195387816
1202	5	0.2697259249243089
1202	6	0.1624530362782508
1202	7	0.20910971006865253
1202	8	0.24150302647214517
1202	9	0.7579817509444595
1202	10	-0.15829806286387083
1202	11	0.3110375850168978
1202	12	0.3388485504428189
1202	13	0.264517109440078
1202	14	0.9004356713441055
1202	15	0.613242182507733
1202	16	0.48520961368438265
1202	17	-1.465344822267511
1202	18	0.022309764829159917
1202	19	0.4055602085880281
1202	20	0.6903082104894551
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2210	19	0.8014188941148177
2210	20	0.9941568559393588
2210	21	0.9931044425631015
2210	22	1.2430177037145214
2210	23	1.525033316430985
2210	24	1.184690090396792
2210	25	1.2235221489328723
2211	1	0.1373159338385328
2211	2	-0.5335210044359897
2211	3	-0.08207421599107434
2211	4	0.33683553800248234
2211	5	0.2713277913461951
2211	6	0.2304893593834123
2211	7	0.26151944868216503
2211	8	0.31771554504894794
2211	9	0.8156027186863994
2211	10	0.880520999117067
2211	11	0.4727458855676474
2211	12	0.32980689470367963
2211	13	0.21985881326033763
2211	14	-0.8677134238565216
2211	15	0.057961563572504815
2211	16	0.279619745688926
2211	17	0.38267237766255846
2211	18	0.3292990121409858
2211	19	0.6518023407674178
2211	20	0.6315387594884925
2211	21	0.625799847424723
2211	22	-0.0198771706382459
2211	23	0.5799657156274094
2211	24	-0.13925161185932922
2211	25	0.36873930971444935
2212	1	0.014736736850732512
2212	2	-1.066188902050122
2212	3	-0.3601470591176792
2212	4	0.14664745647031907
2212	5	0.18792947122744863
2212	6	0.21815743801923304
2212	7	0.16805758995148778
2212	8	0.1854075185473718
2212	9	-2.125877676664963
2212	10	0.018325387082747113
2212	11	0.12502313838253087
2212	12	0.1213897483295483
2212	13	0.10397452232228233
2212	14	0.7571953412135315
2212	15	0.44113903723385306
2212	16	0.42278147046355485
2212	17	0.426631683987452
2212	18	0.22519977605250574
2212	19	-0.5992215178908538
2212	20	0.3802265332837962
2212	21	0.37718466332566436
2212	22	-1.572963590391332
2212	23	-0.2700873237267061
2212	24	0.4198818373278854
2212	25	-0.24471096362830025
2213	1	0.2390901124068563
2213	2	0.5664459195881335
2213	3	0.20210114660131495
2213	4	0.4550315590984084
2213	5	0.4268811298526458
2213	6	0.3521081647534672
2213	7	0.34389095221096966
2213	8	0.47171678939272255
2213	9	0.8226985940762575
2213	10	0.8328870199433496
2213	11	0.29534630441016674
2213	12	0.262859162135733
2213	13	0.22482795932692312
2213	14	-1.5940149365431395
2213	15	-0.22725299584890024
2213	16	0.149025286913556
2213	17	-1.9557205213888054
2213	18	-0.6157927706960606
2213	19	-0.2923111242106984
2213	20	0.47384033609003495
2213	21	-0.11238363036633814
2213	22	0.5643678991738383
2213	23	1.069601670171913
2213	24	0.7854922540551428
2213	25	-0.665617421476334
2214	1	0.4106381697229473
2214	2	0.4044849171627547
2214	3	0.28612966929977546
2214	4	0.6467431529732863
2214	5	0.5623125888276164
2214	6	0.5717236028555277
2214	7	0.5433798151544522
2214	8	0.4667114528030041
2214	9	0.9796249668519863
2214	10	0.9060709566261735
2214	11	0.289574057107572
2214	12	0.1420969889234911
2214	13	0.30559981458561936
2214	14	1.3003541551684137
2214	15	0.8190369211693067
2214	16	0.8722102799102558
2214	17	0.6999773449300122
2214	18	0.639352561031167
2214	19	-0.46129557868286497
2214	20	0.4652338893853667
2214	21	0.7943173219610031
2214	22	0.9957123283144043
2214	23	1.4711813597751302
2214	24	1.1883443897051649
2214	25	1.196221880554397
2215	1	0.030858806736244304
2215	2	0.46542026934399416
2215	3	0.30879978456034607
2215	4	0.5788482206603263
2215	5	0.5674292526089459
2215	6	0.5279969414071246
2215	7	0.4584811249986842
2215	8	0.4264114369706462
2215	9	0.5691014779916088
2215	10	0.8677115146946832
2215	11	0.5607249316112046
2215	12	0.43339663829342356
2215	13	0.42289713694382414
2215	14	1.257944144381689
2215	15	0.7447385142204297
2215	16	0.7100691529125314
2215	17	0.7934526368299117
2215	18	0.7212482815096095
2215	19	-0.03302258341234676
2215	20	-1.2771847034285173
2215	21	-1.5036459755694906
2215	22	0.11739881502484195
2215	23	0.8244432452662309
2215	24	0.9880988154910995
2215	25	0.2593320861136732
2216	1	0.20280429481163864
2216	2	0.0019973287807211193
2216	3	0.04848843416170112
2216	4	0.49310111696049574
2216	5	0.4995031192526511
2216	6	0.4262419892436995
2216	7	0.41491958785289285
2216	8	0.2843493044468559
2216	9	0.6277892346754825
2216	10	-1.3314115886032525
2216	11	-0.1491586347598357
2216	12	0.14200777482248128
2216	13	0.3686071708124885
2216	14	-0.12834389058383522
2216	15	-0.08405095013075226
2216	16	0.35171469278072465
2216	17	0.744950598609698
2216	18	0.5363282030070309
2216	19	-0.8227011929251162
2216	20	-0.4879512070016858
2216	21	-0.4575660433068194
2216	22	-0.14795321305810205
2216	23	-4.110729068968958
2216	24	-1.7445943294982484
2216	25	-0.6718153711647403
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	9.0
4	29.0
5	73.0
6	100.0
7	386.0
8	257.0
9	750.0
10	2132.0
11	4386.0
12	8288.0
13	13073.0
14	18172.0
15	22980.0
16	26839.0
17	29253.0
18	30680.0
19	31868.0
20	32726.0
21	33473.0
22	34882.0
23	36768.0
24	39637.0
25	43003.0
26	48180.0
27	54793.0
28	63815.0
29	77336.0
30	94431.0
31	116300.0
32	151152.0
33	200360.0
34	268517.0
35	364070.0
36	512872.0
37	754243.0
38	1250324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.481148183354144	21.47327688030508	13.846869002400586	23.198705933940193
2	15.663846758250694	21.5665267020816	40.0557796668797	22.713846872788
3	17.804447509287122	26.60292353917545	30.54749061551027	25.04513833602716
4	12.609877282031379	17.747068373534418	38.508152558674915	31.13490178575929
5	13.466549411954482	37.26225217034256	35.55077025164242	13.720428166060536
6	30.265032387705936	38.75396270345543	17.794144900193363	13.186860008645274
7	25.466854846450293	32.774345301350714	24.40081835756251	17.357981494636487
8	23.704535780055288	36.7186766147224	21.7249747182349	17.851812886987418
9	24.324345377427935	17.238079375141904	22.48493447645659	35.952640770973574
10	15.06061080887413	29.918872507919403	33.9047353311213	21.11578135208516
11	31.677346180974986	26.02023132444228	23.94152310163243	18.360899392950305
12	20.80589921930317	27.542235535827963	31.830169519402197	19.82169572546667
13	25.990326056876224	24.54459525585504	27.229052618846456	22.236026068422277
14	20.95547264775856	23.449052944906946	28.39100984995949	27.204464557375008
15	22.62585185193671	30.846588181925444	24.575514547781033	21.952045418356814
16	22.108956055435616	31.31897837776417	25.749071735680175	20.822993831120037
17	20.182307595386185	30.27838676792321	28.710628500738807	20.8286771359518
18	20.29478052854955	30.21968908688459	29.32866262641813	20.156867758147733
19	21.006686057232944	28.730460056723935	29.68738152447236	20.57547236157076
20	22.346600217709213	28.45148364798546	28.799213644576877	20.40270248972845
21	22.798316750286272	28.988114585774287	27.72725783814974	20.4863108257897
22	21.611299539601212	28.527910502397745	29.425731660216627	20.435058297784416
23	21.896459143897562	28.881384710964177	28.645042050071567	20.577114095066698
24	21.568852934068364	27.922104845997637	29.02682599696932	21.482216222964684
25	22.281232366601802	28.279333433759874	28.274357963744183	21.16507623589414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3181.0
1	3181.0
2	3428.5
3	3676.0
4	3676.0
5	3676.0
6	5515.5
7	7355.0
8	7355.0
9	7355.0
10	11606.5
11	15858.0
12	15858.0
13	15858.0
14	26863.0
15	37868.0
16	37868.0
17	37868.0
18	61419.5
19	84971.0
20	84971.0
21	84971.0
22	123710.5
23	162450.0
24	162450.0
25	162450.0
26	214654.5
27	266859.0
28	266859.0
29	266859.0
30	322526.5
31	378194.0
32	378194.0
33	378194.0
34	427290.5
35	476387.0
36	476387.0
37	476387.0
38	509331.5
39	542276.0
40	542276.0
41	542276.0
42	538626.5
43	534977.0
44	534977.0
45	534977.0
46	511283.5
47	487590.0
48	487590.0
49	487590.0
50	460223.5
51	432857.0
52	432857.0
53	432857.0
54	390662.0
55	348467.0
56	348467.0
57	348467.0
58	300874.0
59	253281.0
60	253281.0
61	253281.0
62	208900.5
63	164520.0
64	164520.0
65	164520.0
66	127531.0
67	90542.0
68	90542.0
69	90542.0
70	67140.5
71	43739.0
72	43739.0
73	43739.0
74	31500.0
75	19261.0
76	19261.0
77	19261.0
78	13493.0
79	7725.0
80	7725.0
81	7725.0
82	5299.5
83	2874.0
84	2874.0
85	2874.0
86	1898.0
87	922.0
88	922.0
89	922.0
90	591.0
91	260.0
92	260.0
93	260.0
94	156.0
95	52.0
96	52.0
97	52.0
98	34.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0795894239283141
2	0.017589835273941073
3	0.028468965163422854
4	0.034721601920956593
5	0.04282941661753881
6	0.017566931842594793
7	0.018986944586063995
8	0.030965439180167094
9	0.031858673002671915
10	0.021300191152038017
11	0.02168954948492473
12	0.016009498511047927
13	0.02359053428666576
14	0.033668044079027834
15	0.03213351417882725
16	0.02340730683589554
17	0.023086658797047657
18	0.02473570585397963
19	0.018437262233753336
20	0.01607820880508676
21	0.012711404397183976
22	0.01859758625317728
23	0.02029244017280181
24	0.018391455371060784
25	0.10904323663962687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4366158.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.3787181510238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59387395109972	71.21062024222773
2	8.521417754864387	13.698812719307494
3	1.4878392253465789	3.587718292445108
4	0.48361207466323014	1.5548847457515063
5	0.25003264912213363	1.0048651916170908
6	0.1487518406258755	0.7173889363267955
7	0.10346621386279388	0.5821537149561742
8	0.07451681645199723	0.47916529496853283
9	0.052090170795878465	0.3768247041156546
>10	0.2612386102565741	3.642942467329395
>50	0.014249246982806586	0.7811261885551617
>100	0.007852923112941836	1.1180249538979348
>500	6.580008262891245E-4	0.3776752690480553
>1k	3.4330456172695445E-4	0.613078151134686
>5k	5.7217426954217706E-5	0.2547191283186669
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6039	0.13831382190016944	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5038	0.11538748712254573	No Hit
ATTCCATTCCATTCCATTCCATTCC	4557	0.10437093664498627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.2903431346277436E-5	0.0
9	0.0	0.0	0.0	2.2903431346277436E-5	0.0
10	0.0	0.0	0.0	4.580686269255487E-5	0.0
11	0.0	0.0	0.0	4.580686269255487E-5	0.0
12	0.0	0.0	0.0	6.871029403883232E-5	0.0
13	0.0	2.2903431346277436E-5	0.0	6.871029403883232E-5	2.2903431346277436E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAG	145	7.2759576E-12	12.458765	1
CGCAGAT	150	2.4543624E-8	10.131879	8
GTGGTAT	1840	0.0	9.818048	1
GGTATCA	3420	0.0	9.813818	1
TGGTATC	1775	0.0	9.742238	2
TCTAGAC	320	0.0	9.501467	3
ACACCGT	185	6.6029315E-8	8.728177	6
TAGACAG	710	0.0	8.698526	5
CGTCAGA	220	1.6043487E-9	8.637598	2
TAGTACG	100	0.002902521	8.551321	4
CTAGGAC	435	0.0	8.300132	3
TATTCCG	275	2.1827873E-11	8.29219	5
CTAGTAC	310	0.0	8.275472	3
CCTACAC	425	0.0	8.271866	3
GTACTAG	345	0.0	8.267831	1
GTATCAA	5960	0.0	8.21581	1
TTTAGAC	455	0.0	8.1441145	3
TAGGACT	610	0.0	8.099611	4
GTCTAGA	355	0.0	8.034933	1
GTATAGA	620	0.0	7.974456	1
>>END_MODULE