FastQCFastQC Report
Wed 25 May 2016
SRR1295315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4366158
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT140250.32122062463154105No Hit
GGTATCAACGCAGAGTACTTTTTTT112960.2587171604875499No Hit
TATCAACGCAGAGTACTTTTTTTTT80890.18526585616003818No Hit
GTGGTATCAACGCAGAGTACATGGG66110.15141458463024013No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53290.12205238564431246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC657.985825E-411.6997423
GGTATCA48100.011.4968451
AGCGTCA3600.011.3544383
GTGGTAT18700.011.1976971
TAGACCG600.005851794511.0901265
TAAACCG600.005851794511.0901265
ACCGTCC600.005893360411.0795858
CAGCGTC4000.010.6942952
GCAGCGT3750.010.4063931
TGGTATC19500.010.2372752
TCTAGAC2900.09.5060413
CGTAGTA900.00110790279.5060412
AACACCG1002.7404868E-49.5058225
ACTGCCG900.00111781489.4967888
GCGTATG1252.6956082E-59.13732051
GTATTGG5150.09.0560021
CACCGTC950.00183045848.9969567
CTTACAC6600.08.9299173
TAACCCG1606.6896973E-78.9117095
GTATTAA6550.08.8641311
Produced by FastQC (version 0.11.5)