FastQCFastQC Report
Wed 25 May 2016
SRR1295314_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295314_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3795165
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1321443.481903948840169No Hit
GCTTATGTACTCTGCGTTGATACCA794002.092135651546112No Hit
CTTATGTACTCTGCGTTGATACCAC500851.319705467351222No Hit
GAGTACATAAGCAGTGGTATCAACG325080.8565635486204157No Hit
TATGTACTCTGCGTTGATACCACTG312310.8229154727133077No Hit
CCCATGTACTCTGCGTTGATACCAC290730.7660536498413113No Hit
GCGTTGATACCACTGCTTATGTACT268040.706267052947632No Hit
CATAAGCAGTGGTATCAACGCAGAG214940.566352187586047No Hit
CATGTACTCTGCGTTGATACCACTG207950.5479340160440982No Hit
ACTCTGCGTTGATACCACTGCTTAT205370.5411358926423488No Hit
GTACATGATAAGCAGTGGTATCAAC191080.503482720777621No Hit
ATCATGTACTCTGCGTTGATACCAC187820.494892843921147No Hit
CTGCTTATGTACTCTGCGTTGATAC180270.4749991107106015No Hit
ACGCAGAGTACATAAGCAGTGGTAT177030.4664619324851489No Hit
GTATCAACGCAGAGTACATAAGCAG173660.45758221315805764No Hit
TATCAACGCAGAGTACATAAGCAGT146830.38688699964296674No Hit
GGTATCAACGCAGAGTACATAAGCA132100.34807445789577No Hit
AAGCAGTGGTATCAACGCAGAGTAC114800.30249014206233454No Hit
GTACATGGGAAGCAGTGGTATCAAC100060.26365125099962716No Hit
GTGGTATCAACGCAGAGTACATAAG94360.24863214115855306No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA89790.2365905039701831No Hit
GCAGTGGTATCAACGCAGAGTACAT88030.2319530244403076No Hit
GTACTCTGCGTTGATACCACTGCTT83330.21956884615029912No Hit
GTACATGGGATAAGCAGTGGTATCA82720.2179615379041491No Hit
ACATAAGCAGTGGTATCAACGCAGA77470.20412814726105452No Hit
GCTTATCCCATGTACTCTGCGTTGA73830.19453699641517563No Hit
GAGTACATGATAAGCAGTGGTATCA73390.19337762653270674No Hit
CCACTGCTTATGTACTCTGCGTTGA72370.1906899963506198No Hit
GATACCACTGCTTATGTACTCTGCG66020.17395818100135305No Hit
ATACCACTGCTTATGTACTCTGCGT63790.16808228364247668No Hit
GTGGTATCAACGCAGAGTACATGGG63470.16723910554613566No Hit
GTTGATACCACTGCTTATGTACTCT63060.16615878361019878No Hit
GTACTTTTTTTTTTTTTTTTTTTTT62460.1645778246795594No Hit
GCTTATCATGTACTCTGCGTTGATA60090.15833303690353384No Hit
GTATCAACGCAGAGTACTTTTTTTT58380.1538273039512116No Hit
GATAAGCAGTGGTATCAACGCAGAG56800.1496641121005279No Hit
GCGTTGATACCACTGCTTATCATGT56250.14821489974744181No Hit
CAGTGGTATCAACGCAGAGTACATA51050.1345132556819005No Hit
TACCACTGCTTATGTACTCTGCGTT49820.1312722898740898No Hit
GGATAAGCAGTGGTATCAACGCAGA48040.12658211171319297No Hit
CCCCATGTACTCTGCGTTGATACCA47960.1263713171891077No Hit
TGATACCACTGCTTATGTACTCTGC47000.1238417829000847No Hit
ACGCAGAGTACATGATAAGCAGTGG43730.11522555672810009No Hit
GGGATAAGCAGTGGTATCAACGCAG43630.11496206357299353No Hit
GAGTACATGGGAAGCAGTGGTATCA42900.11303856354071562No Hit
GAGTACATGGGATAAGCAGTGGTAT41190.10853283058839339No Hit
TATCAACGCAGAGTACATGATAAGC40910.10779504975409501No Hit
GCAGAGTACATAAGCAGTGGTATCA40790.10747885796796713No Hit
GTATCAACGCAGAGTACATGATAAG40310.10621409082345563No Hit
GGTATCAACGCAGAGTACTTTTTTT38620.10176105650215471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAC400.005277822714.2512763
AGTCGCG400.00528475114.24845810
TTCGTTG3950.013.70701613
TCTTCGT4050.013.60328611
GTCGCGG500.001501615913.29838511
TCGTTGA4100.012.97369314
CTCTTCG4500.012.24311910
GGCGTAT550.0030581112.09709551
CTTCGTT4400.012.08928212
TACGGCC752.0730848E-411.401324
GTACAAA11350.011.3890851
CCGGCGG700.001494430110.85525218
ATTAACG1252.2488712E-710.63800817
GAAATCG909.543102E-510.55330116
AAATCGC1002.405843E-510.44804317
TCTACGC1553.6798156E-910.417541518
GCAGTTG5450.010.1089979
CTAGAGC1606.17365E-910.0946543
TACATAA293300.010.0025442
AATCGCC951.648134E-49.99825918
Produced by FastQC (version 0.11.5)