##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295314_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3795165
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.14174640628273	31.0	30.0	33.0	26.0	34.0
2	29.942582470063883	31.0	30.0	34.0	25.0	34.0
3	30.30676900740811	31.0	30.0	34.0	26.0	34.0
4	33.36914758646857	35.0	33.0	37.0	27.0	37.0
5	33.94429991844887	35.0	35.0	37.0	28.0	37.0
6	34.00613991750029	37.0	35.0	37.0	29.0	37.0
7	34.02487665226676	37.0	35.0	37.0	29.0	37.0
8	33.931887809884415	37.0	35.0	37.0	28.0	37.0
9	34.90036691421849	38.0	35.0	39.0	27.0	39.0
10	34.74592040135278	38.0	34.0	39.0	27.0	39.0
11	35.259884879840534	38.0	35.0	39.0	27.0	39.0
12	35.253945217138124	38.0	35.0	39.0	27.0	39.0
13	35.04163481693154	38.0	35.0	39.0	27.0	39.0
14	35.27924056002835	39.0	34.0	41.0	24.0	41.0
15	35.86260808159856	39.0	34.0	41.0	27.0	41.0
16	35.87276679670054	39.0	35.0	41.0	27.0	41.0
17	35.857088426985385	39.0	35.0	41.0	27.0	41.0
18	35.81678583144606	39.0	35.0	41.0	27.0	41.0
19	35.85417287522414	39.0	34.0	41.0	27.0	41.0
20	35.85049635523093	39.0	34.0	41.0	27.0	41.0
21	35.83684451137171	39.0	34.0	41.0	27.0	41.0
22	35.613461338308085	39.0	34.0	41.0	25.0	41.0
23	35.27987294360061	39.0	34.0	40.0	24.0	41.0
24	35.59167888616174	39.0	34.0	41.0	25.0	41.0
25	34.395737734723	38.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5260776936725549
1101	2	0.6612099276492067
1101	3	0.4986437599366198
1101	4	0.3985701405519606
1101	5	0.4307268020350179
1101	6	0.38115959844371616
1101	7	0.35380939773903464
1101	8	0.4361253245392547
1101	9	0.4292988829377862
1101	10	0.75044440957371
1101	11	0.6169332449065053
1101	12	0.6340985557770864
1101	13	0.5388903168159302
1101	14	1.1794235242317939
1101	15	0.8885997480868326
1101	16	0.7266884564888301
1101	17	0.9776605244044703
1101	18	0.9939257524033351
1101	19	0.9255484728696359
1101	20	0.9364680573291793
1101	21	0.9683705520844725
1101	22	0.6797513337735595
1101	23	-1.6185367612199215
1101	24	0.1431281582285706
1101	25	0.7116156739706057
1102	1	0.4704619970697017
1102	2	0.47995988008634427
1102	3	0.3917517189724897
1102	4	0.51695087564147
1102	5	0.3101584505176902
1102	6	0.17970954236406556
1102	7	0.19836113860457516
1102	8	0.3746966770100997
1102	9	0.5456622078336721
1102	10	1.0514086363100432
1102	11	0.6499780598906071
1102	12	0.6052667984543874
1102	13	0.5370391297197372
1102	14	1.2203501896646571
1102	15	0.8663257882643762
1102	16	0.7228678835227029
1102	17	0.997191560245092
1102	18	1.0733220060203337
1102	19	1.0293873261666562
1102	20	0.9824411303531875
1102	21	1.110987152341238
1102	22	0.8527837168373296
1102	23	0.9264813466703004
1102	24	1.0004163836715207
1102	25	0.8079353689465236
1103	1	-0.19836309329992474
1103	2	-1.3345333883340835
1103	3	-0.2528800813855092
1103	4	-0.028713123467021262
1103	5	-0.11157274598313904
1103	6	-0.0037251303202765484
1103	7	0.022449955058469584
1103	8	0.14504011641891879
1103	9	0.7370737848968503
1103	10	0.7747334028116413
1103	11	0.5904455442369851
1103	12	0.3582216589125409
1103	13	0.2807062908945781
1103	14	-1.0305611680040485
1103	15	-0.17085426146503835
1103	16	0.004249999042592378
1103	17	0.4457250997395832
1103	18	0.6114499791636661
1103	19	0.5952711376406441
1103	20	0.7031248053150563
1103	21	0.6867555553584381
1103	22	0.3088672121046727
1103	23	1.1603210285134935
1103	24	0.9468959730207871
1103	25	0.7242067773197292
1104	1	0.1329584778480175
1104	2	0.1701209931151766
1104	3	-0.2315380616973748
1104	4	-0.14882662141462788
1104	5	-0.039682297050681825
1104	6	0.04976837150983471
1104	7	0.0631084523673664
1104	8	0.0497654089396633
1104	9	-2.0858773629069702
1104	10	-0.444220673971472
1104	11	-0.33696495442374896
1104	12	0.18282407914261256
1104	13	0.26815100791917246
1104	14	-1.7800826440004016
1104	15	-0.4245927836783068
1104	16	0.004339517983900976
1104	17	0.35942290592201687
1104	18	0.4308357585266336
1104	19	0.38406366793878277
1104	20	0.5775560834343096
1104	21	0.6553244492362467
1104	22	0.8043572679584585
1104	23	1.1280897839889832
1104	24	0.8473107776431874
1104	25	0.6960747962253251
1105	1	-0.16459544379826596
1105	2	-4.6177519270429457E-4
1105	3	-1.407396854179897
1105	4	-0.7129225872913096
1105	5	-0.44376982054940584
1105	6	-0.2272941364110892
1105	7	-0.1872388138599561
1105	8	-0.1682616093101288
1105	9	0.2792797418920472
1105	10	0.3770572538636898
1105	11	-0.22360599692211025
1105	12	-0.24587291011662415
1105	13	-0.2049030206595006
1105	14	0.5123398102166945
1105	15	0.22810234152499476
1105	16	-0.2687606829433804
1105	17	0.31131491609247774
1105	18	0.26627532984657876
1105	19	0.1604589375668155
1105	20	-0.3513204466229283
1105	21	0.016429212612031563
1105	22	0.18452474255871465
1105	23	0.3213006335337951
1105	24	0.2801716282441191
1105	25	0.5373008618527706
1106	1	-1.4921314102343821
1106	2	-0.9193837392213808
1106	3	-0.9638306527713034
1106	4	-1.3644326904294886
1106	5	-0.8660186116563651
1106	6	-0.6566812095333034
1106	7	-0.5189137515470676
1106	8	-0.6356760354337183
1106	9	-0.3713296554549359
1106	10	-2.0061063309784117
1106	11	-1.214637381578882
1106	12	-0.8256011807959922
1106	13	-0.7114493949001286
1106	14	-0.00789592679991813
1106	15	-0.638866431037556
1106	16	-1.4179050232200368
1106	17	-0.6291664667822872
1106	18	-0.5758457830708181
1106	19	-0.39718120320053174
1106	20	-0.16464617144139737
1106	21	-0.10496095833452301
1106	22	-0.13851053320455975
1106	23	-0.44620977685750773
1106	24	-0.6107033562351418
1106	25	-1.3263687838375233
1107	1	0.0662889875589272
1107	2	0.15232370096908454
1107	3	-0.010789142909462868
1107	4	0.1733661096400425
1107	5	-0.015873512761316988
1107	6	0.06226101832643138
1107	7	-0.04211854334543119
1107	8	-0.085484693416646
1107	9	0.5110067697468139
1107	10	0.12548421042472313
1107	11	0.03256957141945804
1107	12	-0.07153771159872946
1107	13	0.10018406312569539
1107	14	-1.1960685823607236
1107	15	-0.5720334052517231
1107	16	-0.16217184944937912
1107	17	-0.10394200492210359
1107	18	0.24639203567458878
1107	19	0.3072630203880351
1107	20	0.3170863267835031
1107	21	0.42417142301891175
1107	22	-0.32205160198078886
1107	23	0.5558502278624289
1107	24	0.38740668668489064
1107	25	-0.015126513935477703
1108	1	-0.03239643932569791
1108	2	0.28673730522095653
1108	3	-0.07551882738498605
1108	4	-0.24106102163192134
1108	5	-0.31680782822162
1108	6	-0.1038440277791608
1108	7	-0.09934815119076035
1108	8	-0.09787910488385165
1108	9	0.31234036433897927
1108	10	0.4616697626391044
1108	11	0.0891229714941062
1108	12	-0.009700065059590202
1108	13	-0.1575859952937293
1108	14	0.329648568852285
1108	15	0.11606075449602571
1108	16	0.03074474166134422
1108	17	0.3207864878464406
1108	18	0.36035543250928725
1108	19	0.37519129223244363
1108	20	0.41187103317240314
1108	21	0.46444642086323995
1108	22	0.38679335608172494
1108	23	0.16978188404007
1108	24	0.3322588992636497
1108	25	-0.4966156950408518
1109	1	-0.6578652115945722
1109	2	-0.5901187914324808
1109	3	-0.6602572147341306
1109	4	-0.5809690530942078
1109	5	-0.83691899387631
1109	6	-1.0457407229287128
1109	7	-0.7144426045252246
1109	8	-1.142206861838858
1109	9	-0.6609740944969573
1109	10	-0.9836801224847278
1109	11	-1.108596672418237
1109	12	-1.1667000427266103
1109	13	-1.1633605583423403
1109	14	-0.45686236366112354
1109	15	-1.1198404255664656
1109	16	-1.332558689074311
1109	17	-1.3454046953417986
1109	18	-1.7212664063593337
1109	19	-1.9472335122940407
1109	20	-1.7790724727973668
1109	21	-1.507341594728473
1109	22	-1.5839479275052426
1109	23	-1.199914701490087
1109	24	-2.083364143621246
1109	25	-1.1746011470550073
1110	1	0.044943611598355915
1110	2	0.2130842704058864
1110	3	0.015556759972557899
1110	4	0.2517608611068809
1110	5	0.16208760919430176
1110	6	0.09194774445469989
1110	7	0.03891457303187451
1110	8	0.059186435554224204
1110	9	0.2739426065362096
1110	10	0.31731398965170143
1110	11	0.2501374715343587
1110	12	0.16651836357954153
1110	13	0.10769607001268611
1110	14	-2.6984301510553195
1110	15	-0.8112856062435796
1110	16	-0.25555716294786635
1110	17	-0.3055342965557486
1110	18	-2.8508124553348324
1110	19	-0.5608038164191598
1110	20	-1.9432775117700203
1110	21	-0.31090089235868845
1110	22	-1.799536460405612
1110	23	-0.5111985628034077
1110	24	0.12059688950586178
1110	25	0.09361687870971735
1111	1	-0.2504180713301487
1111	2	-1.339096433857371
1111	3	-0.4158732701588903
1111	4	-0.014953016773681327
1111	5	-0.14146908911654776
1111	6	-0.02141383821153653
1111	7	-0.04957049052912055
1111	8	-0.0556551764519142
1111	9	-1.6913886283255053
1111	10	-0.4051935432105793
1111	11	-0.1711186563369651
1111	12	-0.20055994124940213
1111	13	-0.07247569507810425
1111	14	0.9175357967133166
1111	15	0.3135348715324966
1111	16	0.2554260668690773
1111	17	-0.11323333034412997
1111	18	0.07272553614814825
1111	19	-0.13630681637167186
1111	20	0.3334936475256072
1111	21	-0.5767447840121207
1111	22	-0.13016486734375832
1111	23	0.08057388934774679
1111	24	-0.4288109298954197
1111	25	-1.8538033204428075
1112	1	0.08793650292748012
1112	2	0.33373010085450616
1112	3	-0.050290509381198945
1112	4	0.3104444495722518
1112	5	0.12441941891618313
1112	6	0.11153922663515914
1112	7	0.026014660488257846
1112	8	-0.0061460401421413735
1112	9	-0.4226427158896442
1112	10	0.3305084752705483
1112	11	0.09100062170893608
1112	12	0.03548304237999389
1112	13	-0.03159756170273198
1112	14	0.436330480209989
1112	15	0.08405200243117861
1112	16	0.17890328076501305
1112	17	-3.566955289526973
1112	18	-1.010812933996739
1112	19	-1.2303576877920293
1112	20	-0.014668232728467956
1112	21	-0.5331159678927548
1112	22	-1.9312901871018866
1112	23	-1.6098021156971853
1112	24	-0.3463846331903895
1112	25	0.001020996940340524
1113	1	-0.41627861708322556
1113	2	-0.16760288001373524
1113	3	-0.13482266324297854
1113	4	-0.4719655122472375
1113	5	-0.22643704435308365
1113	6	-0.24930290181989534
1113	7	-0.27419269963360193
1113	8	-0.27921090507344815
1113	9	0.16676673917237395
1113	10	0.2921952302091313
1113	11	-0.17978314369515402
1113	12	-0.19636515273987953
1113	13	-0.6154420183135301
1113	14	-1.7439764829789297
1113	15	-1.7130905817322528
1113	16	-0.6113426453381905
1113	17	-0.40148694034051147
1113	18	-0.8233364302891744
1113	19	-0.2633305969592925
1113	20	0.18763583004186302
1113	21	-1.6410889576689414
1113	22	-2.1799417693195196
1113	23	-0.8287994767909552
1113	24	-2.5592999349699994
1113	25	-0.6831422855854399
1114	1	0.11755619033481679
1114	2	0.2911619225869444
1114	3	0.0012889446029440421
1114	4	0.4825792169634866
1114	5	0.12727780914648434
1114	6	0.2642554071541028
1114	7	0.26722194853086734
1114	8	0.19943313182425015
1114	9	0.6532187282647755
1114	10	0.44824853337191684
1114	11	0.42492571674725355
1114	12	0.30435612939722034
1114	13	0.252546929356285
1114	14	1.1906810796403846
1114	15	0.498904160687367
1114	16	0.4501078651466983
1114	17	-0.3359506952057103
1114	18	-0.020872478740187717
1114	19	-0.48584727275273565
1114	20	-1.7311205207223352
1114	21	-0.6696672284241316
1114	22	-1.5282223285416379
1114	23	-2.8205648738498397
1114	24	-1.9767294819316135
1114	25	-1.3489928304899976
1115	1	0.27558395360028953
1115	2	0.3418867014342055
1115	3	0.2621683016144516
1115	4	0.5572422117715234
1115	5	0.4527482291374483
1115	6	0.2828596876439633
1115	7	0.2493375766577799
1115	8	0.3753060099533698
1115	9	0.5937080536050274
1115	10	0.9024589214259748
1115	11	0.6245396402934915
1115	12	0.4451881418650103
1115	13	0.2859903594153508
1115	14	1.0291529488938806
1115	15	0.5049786663589728
1115	16	0.473150253861931
1115	17	0.44400756839478106
1115	18	0.5884154935614845
1115	19	0.05693076575455791
1115	20	0.6414500459658754
1115	21	-0.5195156609478246
1115	22	0.5984644684110734
1115	23	0.6726926609870674
1115	24	0.6936984785044018
1115	25	0.8012673578846119
1116	1	0.29529110251089463
1116	2	0.49988730637369727
1116	3	0.3663755459478679
1116	4	0.7946310469096076
1116	5	0.4496711768115418
1116	6	0.3883325326351539
1116	7	0.34627203472242485
1116	8	0.33796109687489917
1116	9	0.5807117290214094
1116	10	0.5616382228966614
1116	11	0.41500872960738633
1116	12	0.47407307605625704
1116	13	-0.14154504362208087
1116	14	1.0207851841877797
1116	15	0.6447404089570838
1116	16	0.6175241968503826
1116	17	0.7059910622145296
1116	18	0.8098320913819776
1116	19	0.8373126043195853
1116	20	0.7177001243174246
1116	21	0.21201094177011726
1116	22	0.25270015430042747
1116	23	-0.548647065320516
1116	24	-0.23439183653005813
1116	25	-0.8494464964724742
1201	1	0.5076121894747949
1201	2	0.6401111650185918
1201	3	0.4886322919767814
1201	4	0.18540556099171113
1201	5	0.367442288295841
1201	6	0.32794587752733406
1201	7	0.38086925493639967
1201	8	0.2407402344958527
1201	9	0.6358541083845921
1201	10	0.872554082140681
1201	11	0.5513876721890085
1201	12	0.6385403629843225
1201	13	0.5498569183128268
1201	14	1.19109219607234
1201	15	0.817104044063214
1201	16	0.6341665160202439
1201	17	0.6366196620616407
1201	18	0.6395777621636185
1201	19	0.9308135301296403
1201	20	0.9203130116538958
1201	21	0.8568090607125569
1201	22	0.9723682848427728
1201	23	0.6691160059784167
1201	24	0.9441840305077491
1201	25	1.103288344482067
1202	1	0.3779487131058836
1202	2	0.8011668784923032
1202	3	0.5470217271366309
1202	4	0.4229186184558529
1202	5	0.38430462107875485
1202	6	0.22371706991685159
1202	7	0.3474549890837366
1202	8	0.3196711444254845
1202	9	0.8093607415892166
1202	10	0.044419141355497516
1202	11	0.35449100151809887
1202	12	0.45046073935777997
1202	13	0.41917253895685747
1202	14	1.2096048356462603
1202	15	0.9177491925940302
1202	16	0.7299003628422867
1202	17	-1.3278007868711228
1202	18	0.3146745999392522
1202	19	0.733825357681475
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2210	19	0.7175764328932814
2210	20	0.8760841547235785
2210	21	0.9838834145751107
2210	22	1.0757680024300171
2210	23	1.3861005484053095
2210	24	1.0818249742298036
2210	25	1.1447963755434785
2211	1	0.1395896215793364
2211	2	-0.6044079314973416
2211	3	-0.04346695414981738
2211	4	0.32186915966776297
2211	5	0.25820872140318585
2211	6	0.13342069904039988
2211	7	0.23606241426134744
2211	8	0.3674827482879621
2211	9	0.7105980981459297
2211	10	0.834788854369414
2211	11	0.5152844521200208
2211	12	0.2628233742641939
2211	13	0.20133998802618436
2211	14	-0.9099723525552008
2211	15	-0.03608817590276203
2211	16	0.1536659552566917
2211	17	0.21807055261933073
2211	18	0.2474255126782552
2211	19	0.6648876708111615
2211	20	0.7401699194634404
2211	21	0.6684174190285361
2211	22	-0.02141646460039226
2211	23	0.5442400170761204
2211	24	0.019992627097309423
2211	25	0.39799551649359444
2212	1	-0.018305907129438026
2212	2	-0.9917632732171846
2212	3	-0.3116722807287289
2212	4	0.19402174463041177
2212	5	0.1279572902699897
2212	6	0.23421631401239296
2212	7	0.14593082752594455
2212	8	0.07692953207759956
2212	9	-2.21573097217788
2212	10	0.016588218361135887
2212	11	0.04284358481378092
2212	12	0.020141294522460385
2212	13	0.06459405771673232
2212	14	0.6303665675850638
2212	15	0.29322521747750585
2212	16	0.4128430598750015
2212	17	0.36781120925085276
2212	18	-0.015149576229376294
2212	19	-0.5802744160892672
2212	20	0.31961442606696266
2212	21	0.3575063880156506
2212	22	-1.5475158306159926
2212	23	-0.27387974000195925
2212	24	0.326709460594266
2212	25	-0.4480902755070275
2213	1	0.07673666263901424
2213	2	0.30775581151743125
2213	3	0.13656078863679255
2213	4	0.40308732846717277
2213	5	0.25489374612863713
2213	6	0.3118338227514741
2213	7	0.1519516207979592
2213	8	0.20300585705497554
2213	9	0.6996270021821829
2213	10	0.6393483177463892
2213	11	0.04707195125873653
2213	12	0.01562992107724881
2213	13	0.11191081756629018
2213	14	-1.8638107735383613
2213	15	-0.45081916148090784
2213	16	-0.04057168725256588
2213	17	-2.0482379127416834
2213	18	-0.8187302563044909
2213	19	-0.6430807581399733
2213	20	0.1982734910191084
2213	21	-0.27834343648510185
2213	22	0.30232105814948085
2213	23	0.7606691125759752
2213	24	0.48421748096939865
2213	25	-1.0049159869953286
2214	1	0.14067768501185185
2214	2	0.12108795386577498
2214	3	0.16597784947781236
2214	4	0.44786956387147825
2214	5	0.46490383603222085
2214	6	0.3913750750361942
2214	7	0.3056207481179172
2214	8	0.2459896325051787
2214	9	0.7238231630932219
2214	10	0.6207052980045802
2214	11	0.1320259177313119
2214	12	-0.013182474916803244
2214	13	0.10042479035878671
2214	14	1.0493931800518723
2214	15	0.5698361555913749
2214	16	0.5672932372417634
2214	17	0.4498037820893188
2214	18	0.47149589317915286
2214	19	-0.7489591026228481
2214	20	0.3450151398525705
2214	21	0.5461145958813987
2214	22	0.747437505033318
2214	23	1.1679646660467782
2214	24	0.9543080021434207
2214	25	0.9813168643363994
2215	1	-0.2287796986259636
2215	2	0.18711281374918798
2215	3	0.18634758484928682
2215	4	0.5411103892102958
2215	5	0.42914495372719585
2215	6	0.29220331893293405
2215	7	0.2471890402626684
2215	8	0.29827955094852854
2215	9	0.4338673436163134
2215	10	0.7754892205593933
2215	11	0.4403733098478213
2215	12	0.37594408425445636
2215	13	0.14360622969729064
2215	14	1.1122935296595742
2215	15	0.5166474775734571
2215	16	0.4583372749132053
2215	17	0.6603413367351578
2215	18	0.522584901400613
2215	19	-0.23507703599373286
2215	20	-1.6231931835014706
2215	21	-1.8870687154975059
2215	22	-0.16734527082745387
2215	23	0.46000209096187916
2215	24	0.7381252662367572
2215	25	-0.05114275466179663
2216	1	-0.10678117125146969
2216	2	-0.4241819964065421
2216	3	-0.10215277448619275
2216	4	0.2725646858227009
2216	5	0.40347386918400474
2216	6	0.0992205048254391
2216	7	0.15998006021832367
2216	8	0.10263848995248992
2216	9	0.3156373354825206
2216	10	-1.6245513323529863
2216	11	-0.41710606782398685
2216	12	-0.11803240387725822
2216	13	0.06849991467799299
2216	14	-0.47662431343616873
2216	15	-0.34834848802690743
2216	16	-0.02791677341416232
2216	17	0.3569269765008727
2216	18	0.13896570137238484
2216	19	-1.2933662184173045
2216	20	-0.9884615058968933
2216	21	-0.8409029272064572
2216	22	-0.7676058586891585
2216	23	-4.350005378944935
2216	24	-2.019115807944196
2216	25	-1.1670684659974881
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	28.0
5	64.0
6	101.0
7	451.0
8	510.0
9	1123.0
10	2248.0
11	3913.0
12	6367.0
13	10027.0
14	14360.0
15	19748.0
16	25616.0
17	29316.0
18	30384.0
19	30002.0
20	30230.0
21	30170.0
22	30296.0
23	31297.0
24	33218.0
25	35688.0
26	39247.0
27	44720.0
28	52324.0
29	63995.0
30	77773.0
31	97425.0
32	128973.0
33	174331.0
34	236122.0
35	324536.0
36	459400.0
37	673928.0
38	1057223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.60025483914861	19.268597931119594	12.527437927786433	21.603709301945365
2	12.831852343397108	23.410806175409153	39.291201501949914	24.466139979243827
3	19.091418489700153	29.988635087743344	31.34319979210012	19.576746630456384
4	12.505772430240574	19.745612425854585	34.95752123938031	32.79109390452453
5	10.29324193626193	43.89481955853042	34.310463767909646	11.501474737298002
6	29.531322557164174	34.16660034123774	22.814779167947055	13.487297933651027
7	23.106317169373426	37.41252069537514	21.72014212800903	17.761020007242394
8	21.799926824742094	37.64313460029861	24.60744742768935	15.949491147269951
9	26.566215929106495	20.558836197693296	19.090349780711584	33.78459809248863
10	13.964584748306711	29.117233196309883	31.622609106801598	25.295572948581814
11	28.503264704347508	28.303803313357324	22.367481257007373	20.82545072528779
12	24.23759420372356	27.865116185910104	27.23566822579166	20.661621384574676
13	27.199010684376795	22.347342136738305	30.875392513398296	19.5782546654866
14	26.605665167989606	24.58890943829134	25.22883470126277	23.576590692456286
15	28.607899107697587	26.001003250415362	24.21837540386365	21.1727222380234
16	23.1812536767109	27.294821082550197	29.67532666060822	19.848598580130687
17	19.533881698503528	33.0672947854178	28.30437719595792	19.094446320120753
18	20.110317800876555	26.491006202509894	35.427580978969665	17.97109501764389
19	21.694746493546607	32.92493782220511	24.85187888026469	20.528436803983595
20	20.48609070499044	31.208517010381144	29.095472182332372	19.209920102296046
21	21.395174046254564	32.472356522458696	25.519361107436268	20.613108323850472
22	19.52441632613259	30.740258045982312	23.911962332824235	25.823363295060865
23	22.851964370475578	27.025943600730468	25.30895938058161	24.813132648212346
24	19.04862054960848	30.749906240965068	23.433328035914915	26.768145173511538
25	22.65170155162263	31.458684592026437	24.32893323570188	21.56068062064905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10635.0
1	10635.0
2	10900.5
3	11166.0
4	11166.0
5	11166.0
6	17029.0
7	22892.0
8	22892.0
9	22892.0
10	21584.0
11	20276.0
12	20276.0
13	20276.0
14	24481.0
15	28686.0
16	28686.0
17	28686.0
18	41479.5
19	54273.0
20	54273.0
21	54273.0
22	76987.5
23	99702.0
24	99702.0
25	99702.0
26	129162.5
27	158623.0
28	158623.0
29	158623.0
30	189211.0
31	219799.0
32	219799.0
33	219799.0
34	248143.0
35	276487.0
36	276487.0
37	276487.0
38	350370.5
39	424254.0
40	424254.0
41	424254.0
42	681141.0
43	938028.0
44	938028.0
45	938028.0
46	750063.5
47	562099.0
48	562099.0
49	562099.0
50	460891.0
51	359683.0
52	359683.0
53	359683.0
54	295617.5
55	231552.0
56	231552.0
57	231552.0
58	196657.5
59	161763.0
60	161763.0
61	161763.0
62	135347.0
63	108931.0
64	108931.0
65	108931.0
66	83464.5
67	57998.0
68	57998.0
69	57998.0
70	43230.0
71	28462.0
72	28462.0
73	28462.0
74	20327.0
75	12192.0
76	12192.0
77	12192.0
78	8502.0
79	4812.0
80	4812.0
81	4812.0
82	3321.5
83	1831.0
84	1831.0
85	1831.0
86	1260.5
87	690.0
88	690.0
89	690.0
90	457.0
91	224.0
92	224.0
93	224.0
94	154.0
95	84.0
96	84.0
97	84.0
98	53.5
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07823111775113861
2	0.01662641808722414
3	0.02716614429148667
4	0.03343728138302287
5	0.04236969934113537
6	0.019577541424417647
7	0.022107075713440654
8	0.04039350067783614
9	0.04034080204681483
10	0.02434676753184644
11	0.024952801788591536
12	0.01920865100726846
13	0.02706074702944404
14	0.04207985687051815
15	0.03941857600394186
16	0.026691856612294854
17	0.025769630569421883
18	0.032067116976468746
19	0.021184849670567683
20	0.01812832907133155
21	0.015783239990883137
22	0.023187397649377564
23	0.023608986697548066
24	0.022765808601207062
25	0.10637218671651957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3795165.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.26621422472785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.57077831008822	47.71096265740765
2	7.242719725057381	7.715845208891368
3	1.2699456993320857	2.0293559902318408
4	0.48990136987817573	1.0438076526767417
5	0.27246555656837224	0.725660435251531
6	0.1807655277883292	0.5777217196571482
7	0.13263018923507258	0.49452956507230994
8	0.10028557462658458	0.4273466321367669
9	0.0803018480129059	0.38496378950072835
>10	0.5601571823155649	5.709231591125658
>50	0.050104469123592066	1.859547395991749
>100	0.03757645614103901	4.05721035843715
>500	0.005457983845429485	2.058554425094041
>1k	0.00500732462840872	6.051957970117117
>5k	9.513916793976566E-4	3.5651559441242235
>10k+	9.513916793976566E-4	15.588148664283999
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	132144	3.481903948840169	No Hit
GCTTATGTACTCTGCGTTGATACCA	79400	2.092135651546112	No Hit
CTTATGTACTCTGCGTTGATACCAC	50085	1.319705467351222	No Hit
GAGTACATAAGCAGTGGTATCAACG	32508	0.8565635486204157	No Hit
TATGTACTCTGCGTTGATACCACTG	31231	0.8229154727133077	No Hit
CCCATGTACTCTGCGTTGATACCAC	29073	0.7660536498413113	No Hit
GCGTTGATACCACTGCTTATGTACT	26804	0.706267052947632	No Hit
CATAAGCAGTGGTATCAACGCAGAG	21494	0.566352187586047	No Hit
CATGTACTCTGCGTTGATACCACTG	20795	0.5479340160440982	No Hit
ACTCTGCGTTGATACCACTGCTTAT	20537	0.5411358926423488	No Hit
GTACATGATAAGCAGTGGTATCAAC	19108	0.503482720777621	No Hit
ATCATGTACTCTGCGTTGATACCAC	18782	0.494892843921147	No Hit
CTGCTTATGTACTCTGCGTTGATAC	18027	0.4749991107106015	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	17703	0.4664619324851489	No Hit
GTATCAACGCAGAGTACATAAGCAG	17366	0.45758221315805764	No Hit
TATCAACGCAGAGTACATAAGCAGT	14683	0.38688699964296674	No Hit
GGTATCAACGCAGAGTACATAAGCA	13210	0.34807445789577	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	11480	0.30249014206233454	No Hit
GTACATGGGAAGCAGTGGTATCAAC	10006	0.26365125099962716	No Hit
GTGGTATCAACGCAGAGTACATAAG	9436	0.24863214115855306	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8979	0.2365905039701831	No Hit
GCAGTGGTATCAACGCAGAGTACAT	8803	0.2319530244403076	No Hit
GTACTCTGCGTTGATACCACTGCTT	8333	0.21956884615029912	No Hit
GTACATGGGATAAGCAGTGGTATCA	8272	0.2179615379041491	No Hit
ACATAAGCAGTGGTATCAACGCAGA	7747	0.20412814726105452	No Hit
GCTTATCCCATGTACTCTGCGTTGA	7383	0.19453699641517563	No Hit
GAGTACATGATAAGCAGTGGTATCA	7339	0.19337762653270674	No Hit
CCACTGCTTATGTACTCTGCGTTGA	7237	0.1906899963506198	No Hit
GATACCACTGCTTATGTACTCTGCG	6602	0.17395818100135305	No Hit
ATACCACTGCTTATGTACTCTGCGT	6379	0.16808228364247668	No Hit
GTGGTATCAACGCAGAGTACATGGG	6347	0.16723910554613566	No Hit
GTTGATACCACTGCTTATGTACTCT	6306	0.16615878361019878	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6246	0.1645778246795594	No Hit
GCTTATCATGTACTCTGCGTTGATA	6009	0.15833303690353384	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5838	0.1538273039512116	No Hit
GATAAGCAGTGGTATCAACGCAGAG	5680	0.1496641121005279	No Hit
GCGTTGATACCACTGCTTATCATGT	5625	0.14821489974744181	No Hit
CAGTGGTATCAACGCAGAGTACATA	5105	0.1345132556819005	No Hit
TACCACTGCTTATGTACTCTGCGTT	4982	0.1312722898740898	No Hit
GGATAAGCAGTGGTATCAACGCAGA	4804	0.12658211171319297	No Hit
CCCCATGTACTCTGCGTTGATACCA	4796	0.1263713171891077	No Hit
TGATACCACTGCTTATGTACTCTGC	4700	0.1238417829000847	No Hit
ACGCAGAGTACATGATAAGCAGTGG	4373	0.11522555672810009	No Hit
GGGATAAGCAGTGGTATCAACGCAG	4363	0.11496206357299353	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4290	0.11303856354071562	No Hit
GAGTACATGGGATAAGCAGTGGTAT	4119	0.10853283058839339	No Hit
TATCAACGCAGAGTACATGATAAGC	4091	0.10779504975409501	No Hit
GCAGAGTACATAAGCAGTGGTATCA	4079	0.10747885796796713	No Hit
GTATCAACGCAGAGTACATGATAAG	4031	0.10621409082345563	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3862	0.10176105650215471	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.6349315510656323E-5	0.0
2	0.0	0.0	0.0	2.6349315510656323E-5	0.0
3	0.0	0.0	0.0	2.6349315510656323E-5	0.0
4	0.0	0.0	0.0	2.6349315510656323E-5	0.0
5	0.0	0.0	0.0	2.6349315510656323E-5	0.0
6	2.6349315510656323E-5	0.0	0.0	2.6349315510656323E-5	0.0
7	2.6349315510656323E-5	0.0	0.0	2.6349315510656323E-5	0.0
8	2.6349315510656323E-5	0.0	0.0	2.6349315510656323E-5	0.0
9	2.6349315510656323E-5	0.0	0.0	5.2698631021312645E-5	0.0
10	2.6349315510656323E-5	0.0	0.0	5.2698631021312645E-5	0.0
11	2.6349315510656323E-5	0.0	0.0	1.0539726204262529E-4	0.0
12	2.6349315510656323E-5	0.0	0.0	1.3174657755328162E-4	0.0
13	5.2698631021312645E-5	0.0	0.0	1.5809589306393793E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGAC	40	0.0052778227	14.251276	3
AGTCGCG	40	0.005284751	14.248458	10
TTCGTTG	395	0.0	13.707016	13
TCTTCGT	405	0.0	13.603286	11
GTCGCGG	50	0.0015016159	13.298385	11
TCGTTGA	410	0.0	12.973693	14
CTCTTCG	450	0.0	12.243119	10
GGCGTAT	55	0.00305811	12.0970955	1
CTTCGTT	440	0.0	12.089282	12
TACGGCC	75	2.0730848E-4	11.40132	4
GTACAAA	1135	0.0	11.389085	1
CCGGCGG	70	0.0014944301	10.855252	18
ATTAACG	125	2.2488712E-7	10.638008	17
GAAATCG	90	9.543102E-5	10.553301	16
AAATCGC	100	2.405843E-5	10.448043	17
TCTACGC	155	3.6798156E-9	10.4175415	18
GCAGTTG	545	0.0	10.108997	9
CTAGAGC	160	6.17365E-9	10.094654	3
TACATAA	29330	0.0	10.002544	2
AATCGCC	95	1.648134E-4	9.998259	18
>>END_MODULE