FastQCFastQC Report
Wed 25 May 2016
SRR1295314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295314_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3795165
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1421473.745476151893264No Hit
GCTTATGTACTCTGCGTTGATACCA895152.3586589779364004No Hit
CTTATGTACTCTGCGTTGATACCAC587511.5480486355665697No Hit
GAGTACATAAGCAGTGGTATCAACG355990.9380092828638544No Hit
CCCATGTACTCTGCGTTGATACCAC345610.910658693363793No Hit
GCGTTGATACCACTGCTTATGTACT302090.7959864722614168No Hit
TATGTACTCTGCGTTGATACCACTG282570.7445526083846157No Hit
CATAAGCAGTGGTATCAACGCAGAG268020.7062143543166107No Hit
ACTCTGCGTTGATACCACTGCTTAT238180.6275879968328122No Hit
CATGTACTCTGCGTTGATACCACTG237860.6267448187364713No Hit
ATCATGTACTCTGCGTTGATACCAC213560.5627159820455764No Hit
ACGCAGAGTACATAAGCAGTGGTAT210880.5556543654887205No Hit
CTGCTTATGTACTCTGCGTTGATAC208700.5499102147073974No Hit
GTACATGATAAGCAGTGGTATCAAC198490.5230075635710174No Hit
GTATCAACGCAGAGTACATAAGCAG193160.5089633784038375No Hit
GTATCAACGCAGAGTACTTTTTTTT164160.4325503634229341No Hit
TATCAACGCAGAGTACATAAGCAGT161650.4259366852297594No Hit
GTACTTTTTTTTTTTTTTTTTTTTT148390.39099749286262914No Hit
GGTATCAACGCAGAGTACATAAGCA143920.37921934882936575No Hit
AAGCAGTGGTATCAACGCAGAGTAC129710.34177697148872316No Hit
GGTATCAACGCAGAGTACTTTTTTT110980.29242470353726385No Hit
TATCAACGCAGAGTACTTTTTTTTT106300.2800932238782767No Hit
GCAGTGGTATCAACGCAGAGTACAT104780.27608812792065696No Hit
GTACATGGGAAGCAGTGGTATCAAC103590.2729525593748889No Hit
GTGGTATCAACGCAGAGTACATAAG101700.2679725387433748No Hit
ACATAAGCAGTGGTATCAACGCAGA97230.2561943947101114No Hit
GTACATGGGATAAGCAGTGGTATCA87020.22929174357373133No Hit
CCACTGCTTATGTACTCTGCGTTGA85500.22528664761611153No Hit
GTACTCTGCGTTGATACCACTGCTT84980.22391648320955743No Hit
GCTTATCCCATGTACTCTGCGTTGA81290.21419358578612524No Hit
GAGTACATGATAAGCAGTGGTATCA78270.20623609250190705No Hit
GATACCACTGCTTATGTACTCTGCG76680.20204655133571267No Hit
ATACCACTGCTTATGTACTCTGCGT70060.18460330446765819No Hit
GCTTATCATGTACTCTGCGTTGATA69250.18246900991129503No Hit
GTGGTATCAACGCAGAGTACATGGG68060.17933344136552692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65970.17382643442379975No Hit
GATAAGCAGTGGTATCAACGCAGAG65500.17258801659479892No Hit
CAGTGGTATCAACGCAGAGTACATA62520.16473592057262332No Hit
GTTGATACCACTGCTTATGTACTCT62500.164683221941602No Hit
GCGTTGATACCACTGCTTATCATGT61910.1631286123264733No Hit
GGATAAGCAGTGGTATCAACGCAGA58100.15308952311691323No Hit
CCCCATGTACTCTGCGTTGATACCA57940.1526679340687427No Hit
TACCACTGCTTATGTACTCTGCGTT56010.14758251617518606No Hit
ACGCAGAGTACATGATAAGCAGTGG52780.13907168726524408No Hit
GGGATAAGCAGTGGTATCAACGCAG52680.1388081941101375No Hit
TGATACCACTGCTTATGTACTCTGC52230.13762247491215798No Hit
GCAGAGTACATAAGCAGTGGTATCA51020.13443420773536857No Hit
GAGTACATGGGAAGCAGTGGTATCA45440.11973128968042232No Hit
TATCAACGCAGAGTACATGATAAGC44710.11780778964814442No Hit
GTATCAACGCAGAGTACATGATAAG43700.11514650878156814No Hit
GAGTACATGGGATAAGCAGTGGTAT43570.11480396767992959No Hit
CAGTGGTATCAACGCAGAGTACATG42370.11164204981865085No Hit
GCGTTGATACCACTGCTTCCCATGT41250.10869092648145733No Hit
ATCAACGCAGAGTACATAAGCAGTG40900.10776870043858436No Hit
ATGATAAGCAGTGGTATCAACGCAG40130.10573980314426382No Hit
GTATCAACGCAGAGTACATGGGAAG39200.10328931680177278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA250.0060472718.9925811
GTTCGGC400.005294658714.24443611
CCCGCAT500.0015055613.29375658
CTTATAC5800.013.2934131
AGCACGC550.003078839312.08618812
TATTCCG1251.8104402E-811.4102835
CGAATAG752.0828578E-411.39554910
TGTCGTG600.00589541211.07900711
CCCCGGT2250.010.97652919
TATACAC8600.010.8353463
CCCCGTA4800.010.0898115
CAGGACT2850.010.0090214
CCGTACA4750.09.99530517
CCGAATA1151.0321686E-59.9091739
TACATAA299400.09.8102772
ATTATAC3500.09.7802433
GTACATA303950.09.7802361
AGCGTCA2054.0017767E-119.7404853
TCCTATA2151.0913936E-119.7296982
CCCCCGG2350.09.69757418
Produced by FastQC (version 0.11.5)