FastQCFastQC Report
Wed 25 May 2016
SRR1295313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360796
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG55830.16612135934463146No Hit
GTATCAACGCAGAGTACTTTTTTTT35100.10443954348910199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.010.6877631
GTGGTAT13200.010.5861641
TGGTATC12750.010.4318392
CTTAGAC1659.295036E-1010.3644083
GTCTTAG3050.010.2851181
TATACCG800.00451498159.5007085
TACACCG900.00111346469.5007075
TGTAACG900.00111376799.5004242
GTCTAAT3550.09.1042831
ATAGGAC2451.6370905E-118.9190313
ACACCGT1401.0877351E-58.8202476
TAACCCG1304.3677293E-58.7698845
TTGAACG1304.3677293E-58.7698844
TAGGACC2106.377377E-98.5958794
GCGTCAG1554.1748935E-68.5860131
CGTCAGA1554.207708E-68.5810282
TAGTACG1000.0029042658.5506364
GGAACGA1356.813921E-58.44318815
GTCTAGG3300.08.3537081
GTATAGA5050.08.2824061
Produced by FastQC (version 0.11.5)