##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295313_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3360796
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.563960145156088	31.0	30.0	33.0	27.0	34.0
2	30.387783132329364	31.0	30.0	34.0	26.0	34.0
3	30.877231762951396	31.0	31.0	34.0	27.0	34.0
4	34.12239035038128	35.0	35.0	37.0	30.0	37.0
5	34.385222726996815	37.0	35.0	37.0	30.0	37.0
6	34.54178563649802	37.0	35.0	37.0	31.0	37.0
7	34.57542677389523	37.0	35.0	37.0	31.0	37.0
8	34.63627426359707	37.0	35.0	37.0	31.0	37.0
9	35.664875821085246	39.0	35.0	39.0	29.0	39.0
10	35.60271792753859	39.0	35.0	39.0	29.0	39.0
11	35.98271867736096	39.0	35.0	39.0	30.0	39.0
12	35.992436613230915	39.0	35.0	39.0	30.0	39.0
13	35.90215800066413	39.0	35.0	39.0	30.0	39.0
14	36.17547420313521	39.0	36.0	41.0	27.0	41.0
15	36.795530285087224	40.0	36.0	41.0	30.0	41.0
16	36.90171673615417	40.0	36.0	41.0	30.0	41.0
17	36.69122880412855	39.0	36.0	41.0	29.0	41.0
18	36.65083837281406	39.0	36.0	41.0	29.0	41.0
19	36.711321960630755	40.0	36.0	41.0	29.0	41.0
20	36.69955332010631	40.0	36.0	41.0	28.0	41.0
21	36.69181051155738	40.0	36.0	41.0	29.0	41.0
22	36.44549743572654	39.0	36.0	41.0	27.0	41.0
23	36.126254911038934	39.0	35.0	41.0	27.0	41.0
24	36.45545906386463	39.0	36.0	41.0	27.0	41.0
25	35.27512857073146	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5001739923944797
1101	2	0.6968634714953126
1101	3	0.5473338724897943
1101	4	0.3748776579112487
1101	5	0.413476240844588
1101	6	0.3687877163626112
1101	7	0.374182884888107
1101	8	0.48770411068033326
1101	9	0.40773170917540114
1101	10	0.8090672959227945
1101	11	0.6149434352122114
1101	12	0.662040581745245
1101	13	0.5945159392489003
1101	14	1.2114478284225925
1101	15	0.8514425810296089
1101	16	0.8012542021578994
1101	17	1.0752160146310814
1101	18	0.9911599182213422
1101	19	1.0738723598316682
1101	20	1.110591249726994
1101	21	1.0812418115435776
1101	22	0.8913899580940594
1101	23	-1.285699277478848
1101	24	0.41825743760051637
1101	25	0.8608673665671418
1102	1	0.4902151721537251
1102	2	0.5521510029946732
1102	3	0.4783553778221652
1102	4	0.6422831382700238
1102	5	0.3104131175074798
1102	6	0.2634861876264125
1102	7	0.3265695643368147
1102	8	0.4038469807927356
1102	9	0.6830390293845241
1102	10	1.0819816277028877
1102	11	0.763460475370259
1102	12	0.6751231465565439
1102	13	0.6801569454052299
1102	14	1.246047808213035
1102	15	1.0393854318544555
1102	16	0.8356121208353287
1102	17	1.074462282945433
1102	18	1.0623036534484953
1102	19	1.1124207872597722
1102	20	1.211418272654882
1102	21	1.1868938102709308
1102	22	1.0629861182188804
1102	23	1.0536629303385894
1102	24	1.1825214398376929
1102	25	1.132143556921534
1103	1	-0.07181906503391389
1103	2	-1.4605797311713644
1103	3	-0.228833643363771
1103	4	0.056254201890951094
1103	5	-0.003166562940151607
1103	6	0.11986290116730203
1103	7	0.2502263002718692
1103	8	0.46406519398006907
1103	9	1.0564492277614477
1103	10	1.0657907935310504
1103	11	0.7786654040453911
1103	12	0.6618526855679789
1103	13	0.5383737345677915
1103	14	-0.6845437696855683
1103	15	0.1971297205859841
1103	16	0.3048941495566808
1103	17	0.7239277082333757
1103	18	0.9741569929064084
1103	19	0.9287768687291091
1103	20	1.0287279798133113
1103	21	1.141264466685243
1103	22	0.5828544031011376
1103	23	1.345248035988078
1103	24	1.181065515024585
1103	25	1.192127963031723
1104	1	0.129587142461844
1104	2	0.2448099985377432
1104	3	-0.11733308023546485
1104	4	-0.03930857345633143
1104	5	-0.06411758129502942
1104	6	-0.03180191039061242
1104	7	0.10044330699624737
1104	8	0.15151579324199105
1104	9	-1.9779419305358985
1104	10	-0.35080320183486435
1104	11	-0.20320316583688935
1104	12	0.20679938001017462
1104	13	0.2558288759345686
1104	14	-1.3370988640982446
1104	15	-0.21441247553240572
1104	16	0.08363325732899085
1104	17	0.474961090564868
1104	18	0.6414991520890041
1104	19	0.618760654056544
1104	20	0.7140744240475598
1104	21	0.8136188956118247
1104	22	0.9410319477048574
1104	23	1.2975804460453162
1104	24	0.9317053849364214
1104	25	0.9174029938977171
1105	1	-0.07873047959484936
1105	2	0.14366768764230997
1105	3	-1.2099506228231327
1105	4	-0.3559832315021296
1105	5	-0.24945247928668124
1105	6	-0.06605195917065743
1105	7	0.052925518307610275
1105	8	-0.023313805832799517
1105	9	0.4527891446248873
1105	10	0.6477073171536603
1105	11	0.14558448967442672
1105	12	-0.06865869323510765
1105	13	-0.06948387955418411
1105	14	0.7397458275061481
1105	15	0.4488147067552575
1105	16	-0.0711507311010422
1105	17	0.3348314344096295
1105	18	0.37598354437970727
1105	19	0.4478844916097984
1105	20	-0.21839765695608548
1105	21	0.22455995718350863
1105	22	0.3688486965726909
1105	23	0.5621297235980691
1105	24	0.48180258113202257
1105	25	0.6569563523390585
1106	1	-1.3651752052814743
1106	2	-0.9068283109345323
1106	3	-0.9551742193694359
1106	4	-1.1148844563849636
1106	5	-0.8102669414333832
1106	6	-0.6651541493477637
1106	7	-0.5979895868527052
1106	8	-0.5588483119422989
1106	9	-0.17580728326791473
1106	10	-1.8479364488701648
1106	11	-1.030513512513643
1106	12	-0.7678850835145141
1106	13	-0.7911727641252
1106	14	-0.11885919491066232
1106	15	-0.9241816680301369
1106	16	-1.3039513338552027
1106	17	-0.49300933175424433
1106	18	-0.4666587673439935
1106	19	-0.40688185164493973
1106	20	-0.18711104421854685
1106	21	-0.11615049798315624
1106	22	-0.19724047054166505
1106	23	-0.42296029700156623
1106	24	-0.6773988087604508
1106	25	-1.3293507751860432
1107	1	-0.04453850993028041
1107	2	0.16636378581155498
1107	3	0.0015994566705828106
1107	4	0.08846417033620924
1107	5	-0.0764907804949786
1107	6	-0.015158339620747086
1107	7	-0.06903895758012624
1107	8	-0.05285755182677576
1107	9	0.4601185209651959
1107	10	-0.051796222382584745
1107	11	0.0514399129347467
1107	12	-0.0817501647060368
1107	13	-0.019534992605485968
1107	14	-0.9245926523115457
1107	15	-0.39305109912408653
1107	16	-0.19223898877891088
1107	17	-1.1882824306042039E-4
1107	18	0.2639118347649756
1107	19	0.24359716972013246
1107	20	0.3791113980853851
1107	21	0.5080428744682592
1107	22	-0.2756462978146246
1107	23	0.42143358333754577
1107	24	0.32523690619989054
1107	25	-0.0037153502191955567
1108	1	-0.1200427869669376
1108	2	0.17703100934343396
1108	3	-0.16945802878437277
1108	4	-0.4753911902225667
1108	5	-0.38102597068576216
1108	6	-0.3640316652925577
1108	7	-0.2505559982105865
1108	8	-0.28917733691661596
1108	9	0.21819099659397523
1108	10	0.25611897097392955
1108	11	-0.09237189375286903
1108	12	-0.08728662224524442
1108	13	-0.2031119698843824
1108	14	0.01545666091298159
1108	15	-0.15103164839120353
1108	16	-0.2521785439755533
1108	17	0.0703580710553311
1108	18	0.08270879053911528
1108	19	0.08497877768297002
1108	20	0.1681468722140096
1108	21	0.1466554080578817
1108	22	0.08005808471108367
1108	23	-0.09901469756352554
1108	24	-0.11812974964129097
1108	25	-0.9735417028744848
1109	1	-0.6484105291018096
1109	2	-0.5969511287501099
1109	3	-0.6214978868585739
1109	4	-0.4824375302762647
1109	5	-0.8096845479359942
1109	6	-1.066313750413336
1109	7	-0.8275162548113073
1109	8	-1.0170643932358274
1109	9	-0.664776436542148
1109	10	-0.8956246503353285
1109	11	-1.0415360003403578
1109	12	-1.10758835283346
1109	13	-1.1196971147922739
1109	14	-0.5546729149687408
1109	15	-1.2163350841927638
1109	16	-1.2638490891666905
1109	17	-1.3140608266665694
1109	18	-1.941204725594531
1109	19	-2.047952090961921
1109	20	-1.651443792542672
1109	21	-1.489335593349665
1109	22	-1.6548743289275833
1109	23	-1.0206217499897505
1109	24	-1.9881912050964559
1109	25	-1.0855123073278818
1110	1	0.0038039721671090376
1110	2	0.1188944562043801
1110	3	-0.12676237931252743
1110	4	0.1605397592202138
1110	5	0.1178082232493054
1110	6	0.11884225812737981
1110	7	-0.008563136544957217
1110	8	0.041058120073678595
1110	9	0.24086396379870223
1110	10	0.2001527574013835
1110	11	0.13500902616983268
1110	12	0.1585598316104111
1110	13	0.042144844055997055
1110	14	-2.626878183651023
1110	15	-0.8133312491392459
1110	16	-0.1888291194640459
1110	17	-0.25956452160569654
1110	18	-3.000053997326141
1110	19	-0.7012392835808612
1110	20	-2.2510520027567154
1110	21	-0.4398368832183337
1110	22	-1.9895110775879274
1110	23	-0.7629575263921993
1110	24	-0.11177203668493263
1110	25	-0.14081991556693652
1111	1	-0.21225794241568607
1111	2	-1.4157500959865779
1111	3	-0.4824960801738385
1111	4	-0.05007203274308125
1111	5	-0.10823594290507543
1111	6	0.0019298567022190127
1111	7	-0.023631122610623834
1111	8	-0.08323747884489308
1111	9	-1.7390182586879277
1111	10	-0.40002464425514006
1111	11	-0.2737633907232393
1111	12	-0.29518064141660716
1111	13	-0.10197933545101279
1111	14	0.7687328811885763
1111	15	0.25007187154346155
1111	16	0.14072234436815734
1111	17	-0.17600850664016576
1111	18	0.11962973691675671
1111	19	-0.21276212848537313
1111	20	0.17533705138755806
1111	21	-0.666154547035994
1111	22	-0.236017165596337
1111	23	-0.10821610149416472
1111	24	-0.6108883335100472
1111	25	-2.2587341777677423
1112	1	0.13989678775351422
1112	2	0.2960156674270955
1112	3	-0.055885385878259086
1112	4	0.24207958276062413
1112	5	0.1614820653579514
1112	6	0.1247908143702503
1112	7	0.057519654681634336
1112	8	-0.021021463051702938
1112	9	-0.3077922484828193
1112	10	0.45436417134749973
1112	11	0.06456256818150052
1112	12	0.046707143598190726
1112	13	0.04728413776744134
1112	14	0.47544189356479194
1112	15	0.05893962193049873
1112	16	0.08797571324532072
1112	17	-3.7775689311507534
1112	18	-1.0257049064082366
1112	19	-1.2065494014125235
1112	20	-0.19094818326583862
1112	21	-0.5717521276737685
1112	22	-1.6802125513110155
1112	23	-1.4819799686261987
1112	24	-0.41260494933437997
1112	25	-0.04170440064954306
1113	1	-0.5969240013650179
1113	2	-0.28887177358616256
1113	3	-0.3526764920428356
1113	4	-0.6895739780380339
1113	5	-0.28824532093523203
1113	6	-0.3342526668159138
1113	7	-0.5355891414071507
1113	8	-0.4500114701914768
1113	9	-0.044503469650521765
1113	10	0.13588659829076732
1113	11	-0.2640466574399625
1113	12	-0.410784908026919
1113	13	-0.7299742728883558
1113	14	-1.715385258012411
1113	15	-2.051705214590548
1113	16	-0.9012732839636044
1113	17	-0.6332671881558198
1113	18	-0.896972149030212
1113	19	-0.446369777630899
1113	20	-0.07837510116073076
1113	21	-1.76611589966042
1113	22	-2.2167709411620535
1113	23	-0.8771513485001634
1113	24	-2.564471861297939
1113	25	-0.9090073695651313
1114	1	0.08662051071067722
1114	2	0.15768414283763477
1114	3	-0.14579046787630645
1114	4	0.2905588944129107
1114	5	0.04466930592636231
1114	6	0.12266180838820873
1114	7	0.09664798443728273
1114	8	0.01701676886757042
1114	9	0.4431001812647679
1114	10	0.3448378258858824
1114	11	0.13012244282833763
1114	12	0.13332793733570725
1114	13	0.11169439023524319
1114	14	0.8841566802822953
1114	15	0.2841235067441161
1114	16	0.19396903706471136
1114	17	-0.48664653646343226
1114	18	-0.3144786402626494
1114	19	-0.47713163780613854
1114	20	-1.7901197810609162
1114	21	-1.0187687452281864
1114	22	-1.6150644955489568
1114	23	-2.9551456858889296
1114	24	-1.949451948013774
1114	25	-1.7907036204265978
1115	1	0.13179903314759756
1115	2	0.23088086827057097
1115	3	0.048903616052356824
1115	4	0.3776665375428081
1115	5	0.28916906037532186
1115	6	0.16388600722304147
1115	7	0.1450666943521739
1115	8	0.10333344964244162
1115	9	0.4123984911120999
1115	10	0.5927298337122693
1115	11	0.27114660055899265
1115	12	0.18406241807093693
1115	13	0.06558382343706626
1115	14	0.7470390051965907
1115	15	0.30278684992878624
1115	16	0.30500173538256803
1115	17	0.23332838120618504
1115	18	0.46504099991476977
1115	19	-0.164022947056921
1115	20	0.3262875802954994
1115	21	-0.5920787878261677
1115	22	0.43153082706994894
1115	23	0.5454205338065066
1115	24	0.4353871794452786
1115	25	0.5483700175462332
1116	1	0.2131821139917065
1116	2	0.4018914137035381
1116	3	0.13477770895058327
1116	4	0.5195670125176051
1116	5	0.35320325894956994
1116	6	0.23998580349582
1116	7	0.06776726830258184
1116	8	0.08526581594717442
1116	9	0.3659173696946709
1116	10	0.10122074543029669
1116	11	0.11654975275227031
1116	12	0.10076833546505526
1116	13	-0.3405035954187028
1116	14	0.6720873648259413
1116	15	0.2514633275463325
1116	16	0.3084164120145729
1116	17	0.5076681596759869
1116	18	0.5425818217342027
1116	19	0.5407976131938455
1116	20	0.4370963772930452
1116	21	-0.14087523145820313
1116	22	-0.1940554968043955
1116	23	-1.0321059542849866
1116	24	-0.5602376626787304
1116	25	-1.122194846125744
1201	1	0.5673589498836868
1201	2	0.767083882011633
1201	3	0.7250080269653054
1201	4	0.5647913918831122
1201	5	0.6312427623583048
1201	6	0.4588386886110527
1201	7	0.5421454906112473
1201	8	0.5654604540683721
1201	9	0.9021724477399999
1201	10	1.171877585037599
1201	11	0.8266275673319541
1201	12	0.8699822404433561
1201	13	0.8424495122625046
1201	14	1.4547173988336795
1201	15	0.9934367622623625
1201	16	0.7824940290230558
1201	17	0.9762558787446594
1201	18	0.8801861242979641
1201	19	1.2005473418496422
1201	20	1.2434232805139374
1201	21	1.1719916775153365
1201	22	1.2050544857855314
1201	23	0.7737153456745105
1201	24	1.0521325973028581
1201	25	1.2597284524649623
1202	1	0.4844456195129929
1202	2	0.8207399257522603
1202	3	0.6108644604615847
1202	4	0.41456438183409006
1202	5	0.32219387170720637
1202	6	0.34627944690489443
1202	7	0.3561706624044305
1202	8	0.4328396400165886
1202	9	0.9518252689446953
1202	10	0.18837123886823548
1202	11	0.47783495388458874
1202	12	0.5697929951802578
1202	13	0.49340487486210094
1202	14	1.1297418805269572
1202	15	0.8098950240798786
1202	16	0.6620915535335783
1202	17	-1.377380517550094
1202	18	0.1595410937712458
1202	19	0.7465357813457061
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2210	21	0.8442122812617114
2210	22	1.0318876280939122
2210	23	1.3296136820941484
2210	24	1.07705858143418
2210	25	0.9384621502409729
2211	1	-0.007212268231288732
2211	2	-0.7539602665723564
2211	3	-0.13826095808582295
2211	4	0.16456918781349827
2211	5	0.10786502566344325
2211	6	0.07448678668207975
2211	7	0.1192234888756829
2211	8	0.17337453057268704
2211	9	0.551801815765188
2211	10	0.6634023602548851
2211	11	0.2033660039291405
2211	12	0.07428092555036869
2211	13	-0.041968842368589776
2211	14	-1.1891238527971453
2211	15	-0.2476759100401793
2211	16	0.02350163216367207
2211	17	0.16260362912583304
2211	18	0.15158645025530149
2211	19	0.3698024247727929
2211	20	0.42959794007921914
2211	21	0.40426457148344497
2211	22	-0.23784796579787582
2211	23	0.23243070612803507
2211	24	-0.33122428549148
2211	25	0.1944072176735432
2212	1	-0.05796042038560856
2212	2	-1.2915809296662495
2212	3	-0.3698972233889357
2212	4	0.15467030717367436
2212	5	0.10630398529764307
2212	6	0.12233410792042321
2212	7	0.06053492667170701
2212	8	0.03721985236825276
2212	9	-2.42729827109585
2212	10	-0.15212036292062692
2212	11	-0.07629376302104873
2212	12	-0.1457725021828864
2212	13	-0.08605081952249805
2212	14	0.4709544860264643
2212	15	0.16806478517590762
2212	16	0.2305013207750406
2212	17	0.35455013583707995
2212	18	0.09424997025791981
2212	19	-0.5456719512207826
2212	20	0.3030471427604411
2212	21	0.2720268344593393
2212	22	-1.7215082571920775
2212	23	-0.36442344927127834
2212	24	0.22679643004681083
2212	25	-0.5055821295575527
2213	1	0.14331419030246195
2213	2	0.36938460133838547
2213	3	0.05847081592580139
2213	4	0.25917279240881896
2213	5	0.22482238601608628
2213	6	0.1519360337437945
2213	7	0.07629424864217782
2213	8	0.02518239610153472
2213	9	0.4266247209152283
2213	10	0.5211598980134724
2213	11	-0.036676433105306216
2213	12	-0.02444500618982204
2213	13	0.012085410029655463
2213	14	-1.9401888468703206
2213	15	-0.5184179678534591
2213	16	-0.29456406866161444
2213	17	-2.2503231701804935
2213	18	-1.0574023836994328
2213	19	-0.5804561380156699
2213	20	0.08821612004096835
2213	21	-0.379918571441614
2213	22	0.1848146549921239
2213	23	0.7326218961358677
2213	24	0.4699605054582605
2213	25	-1.1385150188538944
2214	1	0.13230744361009528
2214	2	0.06395101057096397
2214	3	-0.004760211348862953
2214	4	0.37509504898340396
2214	5	0.26007247676473355
2214	6	0.25215552029422383
2214	7	0.19386374958721575
2214	8	0.050981735201055756
2214	9	0.3761187815728704
2214	10	0.4062701106782285
2214	11	-0.24791030175133955
2214	12	-0.2334125369504818
2214	13	-0.09674474605800754
2214	14	0.7117797728740811
2214	15	0.3237432267742193
2214	16	0.2677361904414113
2214	17	0.2741271433717216
2214	18	0.19547184493708158
2214	19	-1.1105221654780877
2214	20	-6.833733550593024E-6
2214	21	0.267145490843248
2214	22	0.6315986481828446
2214	23	1.0953750222702254
2214	24	0.7109762018445878
2214	25	0.738600926621821
2215	1	-0.33036568217281825
2215	2	0.08682411249363398
2215	3	-0.03289583661362272
2215	4	0.216301715090637
2215	5	0.16971684286579602
2215	6	0.17667285587338455
2215	7	0.09617311281191121
2215	8	-0.04009450838776729
2215	9	0.11157009909306481
2215	10	0.46171560468132355
2215	11	0.12373258218374161
2215	12	-0.037082035167536276
2215	13	-0.17521082558744183
2215	14	0.6155991053332173
2215	15	0.13251601153763204
2215	16	0.19358239499447194
2215	17	0.42558484249607176
2215	18	0.20987607689504983
2215	19	-0.465287971120226
2215	20	-1.8381775113139227
2215	21	-2.1370931651779728
2215	22	-0.4041939921452169
2215	23	0.30935793388723454
2215	24	0.38853394919493667
2215	25	-0.35915656433201804
2216	1	-0.2444652047530056
2216	2	-0.5666056763941789
2216	3	-0.32310350315120573
2216	4	0.11202853795575152
2216	5	0.08983366304025253
2216	6	-0.08538972266938316
2216	7	-0.11718935200700287
2216	8	-0.20745196324723736
2216	9	0.0415300171638151
2216	10	-1.8069238759376063
2216	11	-0.7090238057524374
2216	12	-0.4201739979851027
2216	13	-0.2982158221499276
2216	14	-0.841119065729842
2216	15	-0.7240710509376171
2216	16	-0.2834749793956348
2216	17	0.04751554744747466
2216	18	-0.10693990945057408
2216	19	-1.7463974478865936
2216	20	-1.3300810880623715
2216	21	-1.143220205098764
2216	22	-0.9739228453372775
2216	23	-4.789151642617565
2216	24	-2.2237131692395025
2216	25	-1.607099725193983
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	18.0
5	41.0
6	42.0
7	236.0
8	134.0
9	329.0
10	963.0
11	2131.0
12	4027.0
13	6498.0
14	9329.0
15	11917.0
16	14069.0
17	15636.0
18	16362.0
19	16752.0
20	17627.0
21	17759.0
22	18718.0
23	19681.0
24	21927.0
25	24360.0
26	27980.0
27	33005.0
28	39273.0
29	49333.0
30	61369.0
31	77308.0
32	104902.0
33	143470.0
34	198576.0
35	282466.0
36	412315.0
37	629033.0
38	1083200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36097214082534	20.75569857583251	13.70743340146848	23.175895881873668
2	15.99710374554863	21.31639704993926	39.9603357179122	22.726163486599912
3	18.14889697730201	25.948944182310147	30.324961538037982	25.577197302349862
4	13.099232678564679	17.5043286287184	38.32746608310669	31.068972609610224
5	13.76115546506159	37.12434585364983	35.266451148142195	13.848047533146394
6	30.804809094426094	38.197631163884175	17.749724726958902	13.247835014730827
7	26.035369655305818	32.41205574031501	23.938367222433023	17.614207381946148
8	23.618401999888682	36.59675449663922	21.5608906159825	18.223952887489595
9	24.495249388501264	17.256675887513996	22.21682504022587	36.031249683758865
10	15.049886240028332	29.763388187116924	33.95011227194065	21.236613300914097
11	31.73940785659611	25.704763979510332	23.87677031735658	18.67905784653698
12	20.96674356428808	27.379243355974086	31.465262749411366	20.188750330326467
13	26.282431693224705	24.214493661128873	26.86774827369257	22.63532637195385
14	21.04662227583807	23.462401289637803	28.021643579804522	27.469332854719596
15	22.708327938607574	30.701629847105288	24.380558985857036	22.209483228430102
16	22.10028395165709	31.02510387404582	25.635855585538316	21.238756588758775
17	20.363525327847775	29.97179247259602	28.328416807138844	21.33626539241736
18	20.466872220998685	29.875804310688626	29.022327248052093	20.634996220260593
19	21.28217344892349	28.683409602165067	29.247353555361588	20.78706339354985
20	22.44139231800945	28.38588683986474	28.543816676402745	20.628904165723068
21	22.923409391467477	28.68746451327992	27.459460328078816	20.929665767173784
22	21.89824031997429	28.222464266264318	29.05454026015767	20.82475515360373
23	22.192197625861258	28.582427040230847	28.184951645850493	21.040423688057405
24	21.800871000400562	27.84710413496042	28.611506085515646	21.74051877912337
25	22.418740754994165	28.14178286903025	27.9939223488019	21.44555402717369
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2312.0
1	2312.0
2	2569.0
3	2826.0
4	2826.0
5	2826.0
6	4076.5
7	5327.0
8	5327.0
9	5327.0
10	8592.0
11	11857.0
12	11857.0
13	11857.0
14	20315.5
15	28774.0
16	28774.0
17	28774.0
18	46459.0
19	64144.0
20	64144.0
21	64144.0
22	92878.0
23	121612.0
24	121612.0
25	121612.0
26	159542.0
27	197472.0
28	197472.0
29	197472.0
30	237848.5
31	278225.0
32	278225.0
33	278225.0
34	313994.0
35	349763.0
36	349763.0
37	349763.0
38	375402.5
39	401042.0
40	401042.0
41	401042.0
42	401090.0
43	401138.0
44	401138.0
45	401138.0
46	389237.5
47	377337.0
48	377337.0
49	377337.0
50	361450.5
51	345564.0
52	345564.0
53	345564.0
54	315585.5
55	285607.0
56	285607.0
57	285607.0
58	248134.0
59	210661.0
60	210661.0
61	210661.0
62	174738.0
63	138815.0
64	138815.0
65	138815.0
66	107551.0
67	76287.0
68	76287.0
69	76287.0
70	56637.5
71	36988.0
72	36988.0
73	36988.0
74	26434.0
75	15880.0
76	15880.0
77	15880.0
78	11029.0
79	6178.0
80	6178.0
81	6178.0
82	4171.5
83	2165.0
84	2165.0
85	2165.0
86	1400.0
87	635.0
88	635.0
89	635.0
90	391.5
91	148.0
92	148.0
93	148.0
94	89.0
95	30.0
96	30.0
97	30.0
98	19.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08224242114070594
2	0.017674384282771106
3	0.029249023148087538
4	0.03531901370984731
5	0.04332306989177564
6	0.014758408424670822
7	0.016990022601788387
8	0.030022649396154958
9	0.030290443097409067
10	0.019370411057380455
11	0.019757224181414165
12	0.014401350156332012
13	0.021840064080057224
14	0.03252205727452663
15	0.030736765932832578
16	0.02133423153324391
17	0.020828398986430595
18	0.02308976801924306
19	0.015859338085382153
20	0.013181401072841076
21	0.011425864586841927
22	0.015651054095517848
23	0.017495855148601698
24	0.015532034672738245
25	0.10646287367635525
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3360796.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.90584625193719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.44869585145912	73.26371129845903
2	7.940338715256468	13.007203240002013
3	1.3584359250531963	3.337915320615455
4	0.4232319643106415	1.3866068879093105
5	0.22996555861740683	0.9417761843679087
6	0.14376913666317137	0.7065319681984477
7	0.08771022025937247	0.5028785870701395
8	0.06285516848712204	0.41185646129966597
9	0.04652545375175817	0.3429635995613807
>10	0.238110410592274	3.3911267935339606
>50	0.012872949000406909	0.7212579237256719
>100	0.006578696266177997	0.940542173330772
>500	5.823681836290633E-4	0.33756239959673245
>1k	2.91184088279879E-4	0.5416262138521902
>5k	3.6398011034984876E-5	0.1664409484773656
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5583	0.16612135934463146	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3510	0.10443954348910199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.975485569490085E-5	0.0	0.0	0.0
3	0.0	2.975485569490085E-5	0.0	0.0	0.0
4	0.0	2.975485569490085E-5	0.0	2.975485569490085E-5	0.0
5	0.0	2.975485569490085E-5	0.0	2.975485569490085E-5	0.0
6	0.0	2.975485569490085E-5	0.0	2.975485569490085E-5	0.0
7	0.0	2.975485569490085E-5	0.0	2.975485569490085E-5	0.0
8	0.0	2.975485569490085E-5	0.0	2.975485569490085E-5	0.0
9	0.0	2.975485569490085E-5	0.0	1.190194227796034E-4	0.0
10	0.0	2.975485569490085E-5	0.0	1.190194227796034E-4	0.0
11	0.0	2.975485569490085E-5	0.0	1.4877427847450426E-4	0.0
12	0.0	2.975485569490085E-5	0.0	1.4877427847450426E-4	0.0
13	0.0	2.975485569490085E-5	0.0	1.4877427847450426E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2775	0.0	10.687763	1
GTGGTAT	1320	0.0	10.586164	1
TGGTATC	1275	0.0	10.431839	2
CTTAGAC	165	9.295036E-10	10.364408	3
GTCTTAG	305	0.0	10.285118	1
TATACCG	80	0.0045149815	9.500708	5
TACACCG	90	0.0011134646	9.500707	5
TGTAACG	90	0.0011137679	9.500424	2
GTCTAAT	355	0.0	9.104283	1
ATAGGAC	245	1.6370905E-11	8.919031	3
ACACCGT	140	1.0877351E-5	8.820247	6
TAACCCG	130	4.3677293E-5	8.769884	5
TTGAACG	130	4.3677293E-5	8.769884	4
TAGGACC	210	6.377377E-9	8.595879	4
GCGTCAG	155	4.1748935E-6	8.586013	1
CGTCAGA	155	4.207708E-6	8.581028	2
TAGTACG	100	0.002904265	8.550636	4
GGAACGA	135	6.813921E-5	8.443188	15
GTCTAGG	330	0.0	8.353708	1
GTATAGA	505	0.0	8.282406	1
>>END_MODULE