FastQCFastQC Report
Wed 25 May 2016
SRR1295313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360796
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT130630.3886876799424898No Hit
GGTATCAACGCAGAGTACTTTTTTT108260.3221260677529966No Hit
TATCAACGCAGAGTACTTTTTTTTT76480.2275651363546017No Hit
GTGGTATCAACGCAGAGTACATGGG59770.1778447724884224No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49120.14615585117335297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3050.013.7148773
GGTATCA39250.011.5897441
GTGGTAT15600.011.3468131
TAGACCG600.005847021511.0912225
TGGTATC15350.011.0242922
CAGCGTC3850.010.8650322
GCAGCGT3500.010.3323981
GCGTCAG4400.09.5069044
TATTCCG1856.6247594E-99.2498225
AGCACCG1804.1749445E-88.9786085
GCTACCC850.0073943218.9476743
CCAGTAC2900.08.8512543
GTCCTAC1701.5728783E-68.3970741
CTAAGAC4200.08.3751293
GTGTAGG3750.08.37468051
CGTAGTA1252.7233188E-48.36607552
GTATCAA68700.08.2976611
CAGACGA1150.0011031278.2668724
TCTATAC3800.08.2559953
CTAGACT3900.08.0443024
Produced by FastQC (version 0.11.5)