FastQCFastQC Report
Wed 25 May 2016
SRR1295312_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295312_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052984
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA322463.0623447269854056No Hit
GCTTATGTACTCTGCGTTGATACCA192381.8269983209621417No Hit
CTTATGTACTCTGCGTTGATACCAC133731.2700098007187193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA97580.9266997409267378No Hit
GAGTACATAAGCAGTGGTATCAACG87900.8347705188302957No Hit
TATGTACTCTGCGTTGATACCACTG83850.7963083959490364No Hit
CCCATGTACTCTGCGTTGATACCAC77280.7339142854972156No Hit
GCGTTGATACCACTGCTTATGTACT68790.6532862797535386No Hit
CATAAGCAGTGGTATCAACGCAGAG57530.5463520813231729No Hit
CATGTACTCTGCGTTGATACCACTG57500.5460671767092378No Hit
ACTCTGCGTTGATACCACTGCTTAT57280.5439778762070459No Hit
GTATCAACGCAGAGTACTTTTTTTT55010.5224200937526117No Hit
ACGCAGAGTACATAAGCAGTGGTAT50330.4779749739787119No Hit
CTGCTTATGTACTCTGCGTTGATAC50190.47664541911368075No Hit
ATCATGTACTCTGCGTTGATACCAC49640.4714221678582011No Hit
GTACATGATAAGCAGTGGTATCAAC48880.46420458430517464No Hit
GTGGTATCAACGCAGAGTACATGGG48720.46268509303085326No Hit
GTATCAACGCAGAGTACATAAGCAG41550.3945928903003274No Hit
GGTATCAACGCAGAGTACTTTTTTT39720.37721370885027694No Hit
TATCAACGCAGAGTACATAAGCAGT38970.37009109350189556No Hit
GGTATCAACGCAGAGTACATAAGCA37820.35916974996771084No Hit
AAGCAGTGGTATCAACGCAGAGTAC32800.3114957112358782No Hit
TATCAACGCAGAGTACTTTTTTTTT32330.30703220561755923No Hit
GTACATGGGAAGCAGTGGTATCAAC31960.3035183820456911No Hit
GTACTGGTTCACTATCGGTCAGTCA30560.29022283339537924No Hit
GTGGTATCAACGCAGAGTACATAAG28900.2744581114242951No Hit
GTACTCTGCGTTGATACCACTGCTT24390.23162745112936192No Hit
GCAGTGGTATCAACGCAGAGTACAT24340.23115261010613644No Hit
GGATACCACGTGTCCCGCCCTACTC23420.22241553527878868No Hit
ACATAAGCAGTGGTATCAACGCAGA23150.2198513937533714No Hit
ATACAGGGTGACAGCCCCGTACACA22020.2091199866284768No Hit
GGTATCAACGCAGAGTACATGGGGG21790.2069357179216398No Hit
GTACATGGGATAAGCAGTGGTATCA21030.1997181343686134No Hit
GATACCACTGCTTATGTACTCTGCG20740.19696405643390594No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20500.1946848195224239No Hit
CCACTGCTTATGTACTCTGCGTTGA20130.19117099595055576No Hit
GTTAATGATAGTGTGTCGAAACACA19490.18509303085327034No Hit
GCTTATCCCATGTACTCTGCGTTGA19380.18404838060217438No Hit
GAGTACATGATAAGCAGTGGTATCA18940.17986977959779066No Hit
GCTTATCATGTACTCTGCGTTGATA17290.16420002583135165No Hit
GTATCAACGCAGAGTACATGGGGGT17220.16353524839883607No Hit
GTTGATACCACTGCTTATGTACTCT17130.1626805345570303No Hit
ATACCACTGCTTATGTACTCTGCGT17070.1621107253291598No Hit
ACCCTGTATCGCGCGCCTTTCCAGA16630.15793212432477605No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15610.1482453674509774No Hit
ATCATTAACTGAATCCATAGGTTAA15050.14292714799085265No Hit
GGTTAATGAGGCGAACCGGGGGAAC14700.13960326082827468No Hit
GTATTTAGCCTTGGAGGATGGTCCC14250.13532969161924588No Hit
GCGTTGATACCACTGCTTATCATGT13930.13229070907060317No Hit
GGTATCAACGCAGAGTACATGGGAA13930.13229070907060317No Hit
CAGTGGTATCAACGCAGAGTACATA13910.13210077266131298No Hit
ACTTAGATGTTTCAGTTCCCCCGGT13780.1308661860009269No Hit
GAGTACATGGGAAGCAGTGGTATCA13660.12972656754518586No Hit
CCCATATTCAGACAGGATACCACGT13570.1288718537033801No Hit
TGATACCACTGCTTATGTACTCTGC13470.12792217165692926No Hit
TACCACTGCTTATGTACTCTGCGTT13400.12725739422441365No Hit
GATAAGCAGTGGTATCAACGCAGAG13070.12412344347112586No Hit
GTACATGGGGGTTAAGCGACTAAGC13040.12383853885719061No Hit
GGTATCAACGCAGAGTACATGGGAT12920.1226989204014496No Hit
GCCTTGGAGGATGGTCCCCCCATAT12670.12032471528532249No Hit
GGATAAGCAGTGGTATCAACGCAGA12540.11909012862493637No Hit
GTTCACTATCGGTCAGTCAGGAGTA12450.1182354147831306No Hit
ACGCAGAGTACATGATAAGCAGTGG12290.11671592350880926No Hit
GTATCAACGCAGAGTACATGGGATA11740.11149267225332958No Hit
GTATCAACGCAGAGTACATGGGAAG11730.11139770404868449No Hit
CCCCATGTACTCTGCGTTGATACCA11560.10978324456971805No Hit
CAGTGGTATCAACGCAGAGTACATG11320.10750400765823602No Hit
GCCCAGAGCCTGAATCAGTGTGTGT11310.10740903945359094No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG11300.10731407124894585No Hit
TCTAAGTACCCCGAGGAAAAGAAAT11290.10721910304430077No Hit
GCAGAGTACATAAGCAGTGGTATCA11190.1062694209978499No Hit
GCGTTGATACCACTGCTTCCCATGT11190.1062694209978499No Hit
TATCAACGCAGAGTACATGATAAGC11020.10465496151888347No Hit
GGGATAAGCAGTGGTATCAACGCAG10900.10351534306314246No Hit
GTGGATGCCCTGGCAGTCAGAGGCG10590.10057132871914483No Hit
CTATCGGTCAGTCAGGAGTATTTAG10580.10047636051449974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG250.006029069419.000952
TTATAAG250.006029069419.000953
AGTTGTA900.017.94363611
CAGTTGT907.2759576E-1216.88812810
TACCCGA350.002170226316.2849811
GCAGTTG1058.913048E-1114.475549
ATATGCC400.005275854414.2507123
TCGGAAC400.005275854414.2507123
ATGAGTC400.00528251114.2480047
AAATCGC1700.013.96863217
CCGAATG1301.8189894E-1213.88267118
TACACAG551.9583743E-413.8188735
CGGAACT500.001498421213.3006654
AGTGGTC500.001500031713.29877112
AGCAGTT1153.765308E-1013.2167968
CCTACAC655.446669E-513.1545043
AATCGCC1750.013.02674718
CGAATGG1501.8189894E-1212.6751319
TAGGACT751.47675E-512.66734
TTGTATC1352.7284841E-1112.66609813
Produced by FastQC (version 0.11.5)