##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295312_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1052984
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.313125365627588	31.0	30.0	33.0	23.0	34.0
2	29.076531077395288	31.0	28.0	33.0	20.0	34.0
3	29.535057512744732	31.0	30.0	34.0	23.0	34.0
4	32.56110919064297	35.0	33.0	37.0	20.0	37.0
5	33.26428606702476	35.0	33.0	37.0	27.0	37.0
6	33.4662986332176	35.0	35.0	37.0	27.0	37.0
7	33.544331157928326	36.0	35.0	37.0	27.0	37.0
8	33.47351431740653	36.0	35.0	37.0	27.0	37.0
9	34.26483308388352	38.0	34.0	39.0	22.0	39.0
10	34.22359883910867	38.0	33.0	39.0	23.0	39.0
11	34.65442779757337	38.0	34.0	39.0	26.0	39.0
12	34.688596407922624	38.0	34.0	39.0	26.0	39.0
13	34.44351386155915	38.0	34.0	39.0	25.0	39.0
14	34.557732121285795	38.0	33.0	40.0	18.0	41.0
15	35.1306591553148	39.0	34.0	40.0	24.0	41.0
16	35.14072863405332	38.0	34.0	40.0	25.0	41.0
17	35.15283802982761	38.0	34.0	40.0	24.0	41.0
18	35.08662619754906	38.0	33.0	40.0	24.0	41.0
19	35.153404040327295	39.0	34.0	40.0	24.0	41.0
20	35.19518910068909	39.0	34.0	40.0	24.0	41.0
21	35.21974123063598	39.0	34.0	40.0	24.0	41.0
22	34.99772931022694	39.0	33.0	40.0	23.0	41.0
23	34.689338109600904	38.0	32.0	40.0	22.0	41.0
24	35.03492360757618	38.0	33.0	40.0	24.0	41.0
25	33.87154980512524	38.0	31.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.40291806816105336
1101	2	0.6666088773743404
1101	3	0.5384807624110941
1101	4	0.24081767665761333
1101	5	0.27640433340251747
1101	6	0.38666974387921726
1101	7	0.4114750412856125
1101	8	0.46795299031245463
1101	9	0.5381890877887443
1101	10	0.7020862101805108
1101	11	0.6838283548123627
1101	12	0.8111407433028504
1101	13	0.6058568901806325
1101	14	1.1476503346969835
1101	15	0.9005231355465995
1101	16	0.6968619265080562
1101	17	0.931841877609898
1101	18	0.8477437161867698
1101	19	1.0472048656891957
1101	20	0.9355973038153209
1101	21	0.9614761623610946
1101	22	0.6212581871016027
1101	23	-1.8309463984931824
1101	24	-0.005083275485084471
1101	25	0.7463842360124318
1102	1	0.23196262879316976
1102	2	0.24897436848384302
1102	3	0.15014265463619836
1102	4	0.3944189873466897
1102	5	0.3277256734702405
1102	6	0.24682877678544202
1102	7	0.1602830758359488
1102	8	0.11913253645675326
1102	9	0.37310526570228575
1102	10	0.794593174449922
1102	11	0.6024568193667577
1102	12	0.20661213818823398
1102	13	0.5097804037090441
1102	14	0.9478278238424309
1102	15	0.45335535402109883
1102	16	0.5413097156660882
1102	17	0.8786305002102353
1102	18	0.8398545786376204
1102	19	0.9839416644199588
1102	20	0.9341740185224907
1102	21	0.8333299040675044
1102	22	0.8788234768703163
1102	23	0.8114364138461809
1102	24	0.9014968179311253
1102	25	0.7456962393944337
1103	1	-0.20910043207352658
1103	2	-1.5510393450766244
1103	3	-0.3693095379201168
1103	4	-0.0010079435764041023
1103	5	0.056141604863000794
1103	6	0.024915582726912078
1103	7	0.08735068637800225
1103	8	0.1793498560562128
1103	9	1.0261082665519865
1103	10	0.8622310539928719
1103	11	0.7693114126028391
1103	12	0.49567830746197217
1103	13	0.48765092977197355
1103	14	-0.8374258859893899
1103	15	0.11544919062716019
1103	16	0.27036919845870244
1103	17	0.6200635583162963
1103	18	0.8507967450575009
1103	19	0.6534928097263375
1103	20	0.7159070181890641
1103	21	0.8354021754274967
1103	22	0.23002059003139408
1103	23	1.1366799415834237
1103	24	0.9660267385896617
1103	25	1.0689759494198512
1104	1	-0.03782971384148226
1104	2	0.0509391627663085
1104	3	-0.27207456095272065
1104	4	-0.3076359263352799
1104	5	-0.12817845201493583
1104	6	-0.12210795348092773
1104	7	0.024846057639834385
1104	8	0.06454418899949843
1104	9	-2.454780599296072
1104	10	-0.7657498533879235
1104	11	-0.36846575623872013
1104	12	0.0829189129749821
1104	13	0.0881112114954945
1104	14	-1.8204439713951643
1104	15	-0.7481863314204418
1104	16	-0.3517887563055666
1104	17	0.11413136747897568
1104	18	0.2746780716168331
1104	19	0.249472472902319
1104	20	0.37807208269515513
1104	21	0.652826227091623
1104	22	0.754130761405797
1104	23	1.0672933049356672
1104	24	1.0818747974820226
1104	25	0.8902474082500049
1105	1	-0.370941415969142
1105	2	-0.15928843406216586
1105	3	-1.5059727270584737
1105	4	-0.5361775098159711
1105	5	-0.7367789311661568
1105	6	-0.20042457831693383
1105	7	-0.19834785599886118
1105	8	-0.20961658334663724
1105	9	-0.09065954231130036
1105	10	0.34710380584429146
1105	11	-0.49621624109411755
1105	12	-0.373804177550376
1105	13	-0.44341686733628904
1105	14	0.555992769235722
1105	15	0.10120724281947702
1105	16	-0.387188548102948
1105	17	0.09407760557149203
1105	18	0.054981534054796555
1105	19	0.0026721620244458677
1105	20	-0.8272926996903323
1105	21	0.07919772615043286
1105	22	-0.056390600752671105
1105	23	0.11773965711181233
1105	24	-0.0150035893755458
1105	25	0.5102669450704909
1106	1	-1.5690797138414823
1106	2	-0.9821793296584076
1106	3	-1.134791999695782
1106	4	-1.5079581876982076
1106	5	-0.9487408312156376
1106	6	-0.6260183437795774
1106	7	-0.5350314284877271
1106	8	-0.7543036194673647
1106	9	-0.1157554106932892
1106	10	-1.9634788265159244
1106	11	-1.347883113929349
1106	12	-0.9177026189160316
1106	13	-0.8580785447237176
1106	14	-0.018521703797432565
1106	15	-0.813145181722831
1106	16	-1.3028552972370449
1106	17	-0.49665717918852437
1106	18	-0.32796626406494056
1106	19	-0.24824013386867705
1106	20	-0.32658141814653874
1106	21	-0.26084123970805706
1106	22	-0.16648461361758393
1106	23	-0.4107270672820533
1106	24	-0.7898675181236854
1106	25	-1.4079047521091184
1107	1	-0.39260912560618877
1107	2	-0.22834611032416063
1107	3	-0.44576726189368543
1107	4	-0.21560752650349713
1107	5	-0.3981394860263947
1107	6	-0.2159778741033378
1107	7	-0.3268548439004064
1107	8	-0.29142457249974285
1107	9	0.24728893495194626
1107	10	-0.18821377863631028
1107	11	-0.28856083850755
1107	12	-0.37539470737932845
1107	13	-0.13179035502923853
1107	14	-1.2953969887951544
1107	15	-1.0096083600174026
1107	16	-0.4380724654996939
1107	17	-0.22647934061123465
1107	18	-0.15829287145208326
1107	19	-0.1093037453597887
1107	20	-0.10362770177626857
1107	21	0.0049903836389617595
1107	22	-0.5818260408861704
1107	23	0.24590767963996996
1107	24	0.1646610956474035
1107	25	-0.3994118200525776
1108	1	-0.1844629093524084
1108	2	0.0829512009807516
1108	3	-0.05152677627238589
1108	4	-0.8266374449682274
1108	5	-0.29968658596501285
1108	6	-0.2789020544074674
1108	7	-0.06077182327337027
1108	8	-0.2663230153381875
1108	9	0.14958305549443196
1108	10	0.26482272982413235
1108	11	-0.15437255746566336
1108	12	-0.21729850965628827
1108	13	-0.2872611227811106
1108	14	0.0017322660373224608
1108	15	-0.3096612739768432
1108	16	-0.1083106434643284
1108	17	0.1950856675538759
1108	18	-0.07446261016860234
1108	19	0.1869246692192803
1108	20	0.4164361713807878
1108	21	0.331001539725726
1108	22	0.24842836595630757
1108	23	0.049038434304506495
1108	24	0.20026799197947298
1108	25	-0.9413933645900627
1109	1	-0.7932679735983612
1109	2	-0.55686333775256
1109	3	-0.8110797736546296
1109	4	-0.6858394052434775
1109	5	-0.921460258673271
1109	6	-1.0526348497534812
1109	7	-1.0094117198177557
1109	8	-1.5774895023104385
1109	9	-0.774288866034091
1109	10	-0.7581808009538733
1109	11	-0.9446391287487899
1109	12	-0.9693693413788012
1109	13	-0.9022755644680984
1109	14	-0.6095023574301308
1109	15	-1.1369875986610367
1109	16	-1.1494071913286064
1109	17	-1.2989855503888137
1109	18	-1.7593417122936046
1109	19	-1.9409905467598065
1109	20	-1.9716652238868448
1109	21	-1.6712485140314328
1109	22	-1.7662740763383553
1109	23	-0.9333537802222835
1109	24	-2.1440295351154575
1109	25	-1.0138810722448142
1110	1	-0.08526859626290317
1110	2	0.2568374421422277
1110	3	-0.2554332266497852
1110	4	0.1467587966264503
1110	5	0.0777908308034796
1110	6	0.02204542019692468
1110	7	0.03248555989789992
1110	8	0.04454306542853459
1110	9	0.11313770461114814
1110	10	0.1648579360798479
1110	11	0.17980254002197427
1110	12	-0.011862099924236702
1110	13	-0.010187202708934251
1110	14	-2.5919634762748913
1110	15	-0.6113861054578393
1110	16	-0.32736747337479244
1110	17	-0.5031733418438549
1110	18	-2.9635704685211834
1110	19	-0.8720066146332215
1110	20	-2.105256933212736
1110	21	-0.4311193483795748
1110	22	-1.9350161673442017
1110	23	-0.62707432748185
1110	24	0.1039279985668955
1110	25	0.3449971484242198
1111	1	-0.017092386652542757
1111	2	-1.2808812372781695
1111	3	-0.3720656038110981
1111	4	0.1178020470033232
1111	5	-0.16512974987386997
1111	6	-0.01901279775021436
1111	7	0.022599050560607736
1111	8	-0.05461976993355222
1111	9	-1.8155866184952387
1111	10	-0.47376711287323303
1111	11	-0.16672621756239892
1111	12	-0.315814423652931
1111	13	-0.04697175016793409
1111	14	0.8525230663236698
1111	15	0.36543964305479193
1111	16	0.17245884379821774
1111	17	-0.15791401125368765
1111	18	0.15489329010931385
1111	19	-0.22545988523508953
1111	20	0.3275754246355831
1111	21	-0.5011624807325958
1111	22	-0.061820054620696396
1111	23	0.022007232364288143
1111	24	-0.5769214244574172
1111	25	-2.1702225637048755
1112	1	0.2836786660467858
1112	2	0.4998997271169614
1112	3	0.12563089234007307
1112	4	0.3260461321666739
1112	5	0.07732127090207541
1112	6	0.301314545657867
1112	7	0.2851939840106468
1112	8	0.13136612199034658
1112	9	-0.4008632449144187
1112	10	0.5762476903088114
1112	11	0.21706751618628317
1112	12	0.27325246098522626
1112	13	-0.0026072572090996005
1112	14	0.5312290107666868
1112	15	0.35290327871629756
1112	16	0.05296434028407049
1112	17	-3.760446642615836
1112	18	-1.158200446213833
1112	19	-1.1817158386887385
1112	20	0.04311619131168953
1112	21	-0.6726721478540867
1112	22	-1.5732989568682072
1112	23	-1.3096316729725785
1112	24	-0.24457389723243494
1112	25	0.044614086399391795
1113	1	-0.4753978708752875
1113	2	-0.24278805122265723
1113	3	-0.13974685754874017
1113	4	-0.6629829461369994
1113	5	-0.3915650050889852
1113	6	-0.5179442713492577
1113	7	-0.7305977341862899
1113	8	-0.5203125971752627
1113	9	-0.21149543757569234
1113	10	0.01607089324770783
1113	11	-0.39921645571627096
1113	12	-0.6133616498815186
1113	13	-0.854593025715829
1113	14	-1.8187621554725055
1113	15	-2.2299714098174803
1113	16	-0.9190606623051352
1113	17	-0.6579975585854498
1113	18	-1.0274333711634185
1113	19	-0.6637491876588442
1113	20	-0.18504461968847608
1113	21	-2.006395182037693
1113	22	-2.5911187847012513
1113	23	-1.1410859536912241
1113	24	-2.905908830796456
1113	25	-0.9764279318408029
1114	1	0.17545607591023327
1114	2	0.21495991652397706
1114	3	-0.1104228428265337
1114	4	0.47941535190301465
1114	5	0.17736022653691919
1114	6	0.1443531441993926
1114	7	0.09175299146829019
1114	8	0.027677731654883075
1114	9	0.47028833677289583
1114	10	0.2891670905100838
1114	11	0.2650160003103821
1114	12	0.2568261682926547
1114	13	0.22626447546080186
1114	14	1.0673665595718944
1114	15	0.5371923531363194
1114	16	0.39210294856449934
1114	17	-0.41657403934651427
1114	18	-0.2392266910972154
1114	19	-0.3703661735006136
1114	20	-1.5598712927150231
1114	21	-0.5668270319168016
1114	22	-1.3280437236803664
1114	23	-2.862474401401638
1114	24	-1.9682987642077947
1114	25	-1.3359252130484407
1115	1	0.01301774378563536
1115	2	0.14838826986859388
1115	3	0.0982465300637756
1115	4	0.16589380284180066
1115	5	0.16416318928101248
1115	6	-0.11893566739014005
1115	7	0.0923458872395102
1115	8	0.08565252952750768
1115	9	0.173956847879829
1115	10	0.4975726121912061
1115	11	0.21918675205409954
1115	12	-0.06278046676782623
1115	13	0.0887459527141985
1115	14	0.7634057695963392
1115	15	0.1273972897001059
1115	16	0.09367603898684251
1115	17	0.12720959258916764
1115	18	0.37214705875728527
1115	19	-0.49533315712449166
1115	20	0.41797415930714266
1115	21	-0.6444882637522014
1115	22	0.5079463087316469
1115	23	0.41938724095602
1115	24	0.4641480514466707
1115	25	0.20794319822591945
1116	1	0.5500081239963563
1116	2	0.5277775189612335
1116	3	0.42429638206701625
1116	4	0.6325119447777396
1116	5	0.533950267754534
1116	6	0.3631439513420176
1116	7	0.15654498692589414
1116	8	-0.07171405315686741
1116	9	0.33418536192190373
1116	10	0.236510294984825
1116	11	0.09935012109458086
1116	12	0.16357639041530092
1116	13	-0.3183629570430142
1116	14	0.5391147004959507
1116	15	0.3072012380421896
1116	16	0.31209568908022334
1116	17	0.49365793467310226
1116	18	0.4788104366071053
1116	19	0.5520940778027423
1116	20	0.3103637783916824
1116	21	-0.09069848962344196
1116	22	0.08372086914603472
1116	23	-1.0593349215585093
1116	24	-0.5680126131292482
1116	25	-1.1844210940324373
1201	1	0.4345003832458971
1201	2	0.7485301984517747
1201	3	0.6494200120450166
1201	4	0.26191603146337883
1201	5	0.5197847953366335
1201	6	0.09942522888542982
1201	7	0.2711117942111514
1201	8	0.26584389313894263
1201	9	0.6233109699795492
1201	10	0.9920058577619386
1201	11	0.6898812422082301
1201	12	0.7966022467421254
1201	13	0.5849738749345974
1201	14	1.4326048672870897
1201	15	0.8317953616654421
1201	16	0.2897544905254321
1201	17	0.40930259352164455
1201	18	0.351352807207725
1201	19	0.7718593995098146
1201	20	0.8212200042964497
1201	21	0.8117757919770483
1201	22	0.7943530472539422
1201	23	0.5628801239575054
1201	24	0.8763532239550358
1201	25	0.9886830781008626
1202	1	0.38056071925483437
1202	2	0.6595888713608211
1202	3	0.36221458469110246
1202	4	0.47808052564043635
1202	5	0.29449814594946844
1202	6	0.11036612503477983
1202	7	0.35281413907740244
1202	8	0.1782815791917045
1202	9	0.9046836235966325
1202	10	-0.1330061186492344
1202	11	0.34040517097316325
1202	12	0.23234069447372008
1202	13	0.2572017802864295
1202	14	0.9872401045037051
1202	15	0.723254977625345
1202	16	0.4947814452953878
1202	17	-1.355479017405031
1202	18	-0.06939096453547222
1202	19	0.5534305791392455
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2210	21	0.9301224650513689
2210	22	1.1576886881050186
2210	23	1.3537114596670108
2210	24	1.2014218296994201
2210	25	1.0893450114956025
2211	1	0.03181838000016057
2211	2	-0.4816039017105105
2211	3	0.053206704525727844
2211	4	0.2551816029312661
2211	5	0.12067879641854518
2211	6	0.10824945612494474
2211	7	0.18811015236202877
2211	8	0.1217312983529979
2211	9	0.6131523844373135
2211	10	0.4591717224849603
2211	11	0.48040407150337217
2211	12	0.08398658179323348
2211	13	0.10692435372816789
2211	14	-0.852053808414496
2211	15	-0.0385611948170137
2211	16	0.23880781740525237
2211	17	0.15101979687366907
2211	18	0.3336113462467267
2211	19	0.6277240276262432
2211	20	0.6103781058253617
2211	21	0.5510159859619961
2211	22	-0.17441955189761416
2211	23	0.31947267015230807
2211	24	-0.30889670723914975
2211	25	0.24129817362231165
2212	1	-0.030415052397721354
2212	2	-1.038632752961071
2212	3	-0.38422426892705275
2212	4	0.05049083365757667
2212	5	0.21215634607253264
2212	6	0.022197208464667995
2212	7	-0.0318446531984975
2212	8	0.00813440190143666
2212	9	-2.325596185800329
2212	10	-0.15271536690452336
2212	11	-0.22191357943604118
2212	12	-0.12753160018905163
2212	13	-0.1316881736978317
2212	14	0.529138806216757
2212	15	0.17966952657091895
2212	16	0.1422338316134386
2212	17	0.23628893681725316
2212	18	-0.06765357292349705
2212	19	-0.7536221890215273
2212	20	0.12745435051862586
2212	21	0.16711958198869326
2212	22	-1.8417523743182542
2212	23	-0.41475120478676786
2212	24	0.2274165489759099
2212	25	-0.7618572319050614
2213	1	-0.050532114734838984
2213	2	0.2801635540105849
2213	3	-0.06344203280707461
2213	4	0.33449901108641455
2213	5	0.27703273948021945
2213	6	0.13618941659854045
2213	7	-0.07148793583064617
2213	8	0.08524944300260273
2213	9	0.43172520946174586
2213	10	0.4842426620639273
2213	11	-0.10501340362070266
2213	12	0.038784029442034296
2213	13	-0.36019750515897186
2213	14	-2.01153384135862
2213	15	-0.8679102863155634
2213	16	-0.6919685800594237
2213	17	-2.7729473834397567
2213	18	-1.4496218611719272
2213	19	-0.9103596786208641
2213	20	0.033491718856303976
2213	21	-0.6456309829981492
2213	22	0.03338748589808205
2213	23	0.4087469987847143
2213	24	0.1301800712443395
2213	25	-1.6806484771944952
2214	1	0.37128571226423546
2214	2	0.20807712401811784
2214	3	0.37466273049161813
2214	4	0.6210314575690958
2214	5	0.5609929938263889
2214	6	0.428982941938294
2214	7	0.4285012224743667
2214	8	0.34275867199546894
2214	9	0.7801610239169818
2214	10	0.6517685330585934
2214	11	0.20563097252106388
2214	12	0.1315537030528091
2214	13	0.3914463668439794
2214	14	1.2272111240292674
2214	15	0.6530467101012576
2214	16	0.4600889026074171
2214	17	0.31243096461118114
2214	18	0.39633662597795194
2214	19	-1.001064009335927
2214	20	0.1368618597441298
2214	21	0.5191112828076854
2214	22	0.881300188570016
2214	23	1.3863968445616024
2214	24	0.9737642271912819
2214	25	0.9283810284784195
2215	1	-0.020645678364765274
2215	2	0.551637640382328
2215	3	0.3696361444799514
2215	4	0.4590547164424592
2215	5	0.5332887670930333
2215	6	0.3465764713599029
2215	7	0.3603367455957027
2215	8	0.2996978243525703
2215	9	0.4047481215090514
2215	10	0.8678585116988913
2215	11	0.626237702860216
2215	12	0.3957239396360208
2215	13	0.11866886413514521
2215	14	0.9956216082223719
2215	15	0.5033027634120089
2215	16	0.5371949141794445
2215	17	0.6960702224512403
2215	18	0.6211229691635438
2215	19	-0.21997284670320738
2215	20	-1.551298455221776
2215	21	-1.7291964451945674
2215	22	-0.13036333274304468
2215	23	0.7999175596451877
2215	24	0.8921118043122434
2215	25	-0.002078551933799133
2216	1	0.14653009166905306
2216	2	-0.005987170800214159
2216	3	0.0632828938250043
2216	4	0.47487675067576873
2216	5	0.5509450708109256
2216	6	0.13379132855341425
2216	7	-0.06967172348854689
2216	8	-0.09191496719895298
2216	9	0.3209742640268516
2216	10	-1.7460869076285874
2216	11	-0.6493088046307918
2216	12	-0.24101583427416529
2216	13	-0.13988017928956964
2216	14	-0.23937301923974275
2216	15	-0.36406203370729884
2216	16	0.02235035841106736
2216	17	0.3185817551398671
2216	18	0.04488923202139716
2216	19	-1.172028186838162
2216	20	-0.9349946894141112
2216	21	-0.8859221199362182
2216	22	-0.6661673746644112
2216	23	-4.27041596894426
2216	24	-2.0142952316160816
2216	25	-1.1329441714047874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	9.0
6	18.0
7	69.0
8	49.0
9	164.0
10	446.0
11	1022.0
12	2306.0
13	3913.0
14	6388.0
15	9199.0
16	12100.0
17	13137.0
18	12916.0
19	12321.0
20	11589.0
21	10617.0
22	9863.0
23	9783.0
24	9669.0
25	9840.0
26	10705.0
27	11944.0
28	13724.0
29	16984.0
30	20724.0
31	26497.0
32	35132.0
33	47447.0
34	65663.0
35	92246.0
36	130966.0
37	194403.0
38	251127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.18978830573309	19.64047613390672	11.982513245741169	20.18722231461902
2	14.602674356534012	24.008419340928217	37.31922257872711	24.069683723810662
3	21.222101368392554	30.796004540726425	28.99149326253093	18.990400828350094
4	12.811263827086842	22.262227305717794	31.379459722375273	33.54704914482009
5	10.874641828366434	44.60357905617499	31.26828860463172	13.253490510826854
6	28.2542643492896	34.65570348109952	21.66666508367233	15.423367085938548
7	22.847294645520012	39.19748337351046	18.6023870718952	19.352834909074332
8	23.01668653724665	36.65157769238442	22.20511188482347	18.12662388554546
9	26.920519294965693	22.1858381354628	17.51499537361612	33.37864719595538
10	16.324886827972822	30.376834830579146	26.933327761008037	26.36495058044
11	28.13079993237172	30.05165204869996	19.038120693683787	22.779427325244537
12	24.794631010171333	30.62180097847674	23.405083861972283	21.178484149379642
13	28.941304310657628	23.695888813111115	27.650063735649983	19.712743140581278
14	26.838858736698707	28.014398317346284	22.769215569106887	22.37752737684812
15	30.39242975090415	26.162141724623734	22.796151036387315	20.6492774880848
16	22.939221985178317	27.882030657818994	27.537704861975225	21.64104249502746
17	20.405395136778115	33.28324468085106	24.66498860182371	21.646371580547115
18	20.78287731350429	25.62921997241463	33.18451419734557	20.403388516735504
19	22.807190843044378	33.223218364821214	23.457052787748175	20.512538004386236
20	22.43952616322142	28.697385767420304	28.201209666695792	20.66187840266248
21	24.22584064341371	30.969033625736547	23.465263029925424	21.33986270092432
22	22.193927224336697	29.568663284564835	22.043470868553005	26.193938622545467
23	25.232735848966996	25.961267207635235	23.818416170926564	24.9875807724712
24	21.305552072766236	29.85298196421719	21.901868928073434	26.93959703494314
25	24.40419546780721	29.89693857858707	23.50619880083316	22.192667152772565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10367.0
1	10367.0
2	7488.0
3	4609.0
4	4609.0
5	4609.0
6	6098.5
7	7588.0
8	7588.0
9	7588.0
10	7549.5
11	7511.0
12	7511.0
13	7511.0
14	8009.0
15	8507.0
16	8507.0
17	8507.0
18	10946.0
19	13385.0
20	13385.0
21	13385.0
22	16861.5
23	20338.0
24	20338.0
25	20338.0
26	26889.0
27	33440.0
28	33440.0
29	33440.0
30	38766.0
31	44092.0
32	44092.0
33	44092.0
34	48769.5
35	53447.0
36	53447.0
37	53447.0
38	68436.5
39	83426.0
40	83426.0
41	83426.0
42	157923.5
43	232421.0
44	232421.0
45	232421.0
46	201399.0
47	170377.0
48	170377.0
49	170377.0
50	147339.5
51	124302.0
52	124302.0
53	124302.0
54	103672.0
55	83042.0
56	83042.0
57	83042.0
58	71531.0
59	60020.0
60	60020.0
61	60020.0
62	53469.5
63	46919.0
64	46919.0
65	46919.0
66	35831.5
67	24744.0
68	24744.0
69	24744.0
70	19951.0
71	15158.0
72	15158.0
73	15158.0
74	10623.5
75	6089.0
76	6089.0
77	6089.0
78	4199.5
79	2310.0
80	2310.0
81	2310.0
82	1513.0
83	716.0
84	716.0
85	716.0
86	421.0
87	126.0
88	126.0
89	126.0
90	80.0
91	34.0
92	34.0
93	34.0
94	23.0
95	12.0
96	12.0
97	12.0
98	8.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07208086732561939
2	0.01614459478966442
3	0.027445811142429513
4	0.03228918957932884
5	0.03950677313235529
6	0.012060961989925772
7	0.013865357878182384
8	0.03219422137468375
9	0.030959634714297652
10	0.015384849152503741
11	0.01614459478966442
12	0.011681089171345433
13	0.018803704519726794
14	0.03428352187687562
15	0.032004284965393585
16	0.019183577338307136
17	0.017474149654695608
18	0.023931987570561374
19	0.0137703896735373
20	0.012535803013151197
21	0.009496820464508483
22	0.017759054268630864
23	0.017189245040760352
24	0.017854022473275947
25	0.1032304384492072
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1052984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.839399952971792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57034859457549	19.019615132791323
2	8.214816098819492	4.081022052373776
3	3.763341184408732	2.804374105170572
4	2.2289760177348645	2.214657071603946
5	1.5301127131184604	1.9003540827138115
6	1.1026875640419955	1.6434058455844398
7	0.8772721626611943	1.5253639881164955
8	0.6867301435661053	1.3646371756640185
9	0.582618063657666	1.302469479271687
>10	3.893013843283881	18.46030952987151
>50	0.30509160861616985	5.225313486586094
>100	0.1837440041369915	9.641261490406242
>500	0.029475600663642385	5.19507636188642
>1k	0.02641320059469253	12.388083474851936
>5k	0.004210800094806055	7.067114274852592
>10k+	0.0011484000258561967	6.166942448255135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	32246	3.0623447269854056	No Hit
GCTTATGTACTCTGCGTTGATACCA	19238	1.8269983209621417	No Hit
CTTATGTACTCTGCGTTGATACCAC	13373	1.2700098007187193	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9758	0.9266997409267378	No Hit
GAGTACATAAGCAGTGGTATCAACG	8790	0.8347705188302957	No Hit
TATGTACTCTGCGTTGATACCACTG	8385	0.7963083959490364	No Hit
CCCATGTACTCTGCGTTGATACCAC	7728	0.7339142854972156	No Hit
GCGTTGATACCACTGCTTATGTACT	6879	0.6532862797535386	No Hit
CATAAGCAGTGGTATCAACGCAGAG	5753	0.5463520813231729	No Hit
CATGTACTCTGCGTTGATACCACTG	5750	0.5460671767092378	No Hit
ACTCTGCGTTGATACCACTGCTTAT	5728	0.5439778762070459	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5501	0.5224200937526117	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	5033	0.4779749739787119	No Hit
CTGCTTATGTACTCTGCGTTGATAC	5019	0.47664541911368075	No Hit
ATCATGTACTCTGCGTTGATACCAC	4964	0.4714221678582011	No Hit
GTACATGATAAGCAGTGGTATCAAC	4888	0.46420458430517464	No Hit
GTGGTATCAACGCAGAGTACATGGG	4872	0.46268509303085326	No Hit
GTATCAACGCAGAGTACATAAGCAG	4155	0.3945928903003274	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3972	0.37721370885027694	No Hit
TATCAACGCAGAGTACATAAGCAGT	3897	0.37009109350189556	No Hit
GGTATCAACGCAGAGTACATAAGCA	3782	0.35916974996771084	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	3280	0.3114957112358782	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3233	0.30703220561755923	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3196	0.3035183820456911	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3056	0.29022283339537924	No Hit
GTGGTATCAACGCAGAGTACATAAG	2890	0.2744581114242951	No Hit
GTACTCTGCGTTGATACCACTGCTT	2439	0.23162745112936192	No Hit
GCAGTGGTATCAACGCAGAGTACAT	2434	0.23115261010613644	No Hit
GGATACCACGTGTCCCGCCCTACTC	2342	0.22241553527878868	No Hit
ACATAAGCAGTGGTATCAACGCAGA	2315	0.2198513937533714	No Hit
ATACAGGGTGACAGCCCCGTACACA	2202	0.2091199866284768	No Hit
GGTATCAACGCAGAGTACATGGGGG	2179	0.2069357179216398	No Hit
GTACATGGGATAAGCAGTGGTATCA	2103	0.1997181343686134	No Hit
GATACCACTGCTTATGTACTCTGCG	2074	0.19696405643390594	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2050	0.1946848195224239	No Hit
CCACTGCTTATGTACTCTGCGTTGA	2013	0.19117099595055576	No Hit
GTTAATGATAGTGTGTCGAAACACA	1949	0.18509303085327034	No Hit
GCTTATCCCATGTACTCTGCGTTGA	1938	0.18404838060217438	No Hit
GAGTACATGATAAGCAGTGGTATCA	1894	0.17986977959779066	No Hit
GCTTATCATGTACTCTGCGTTGATA	1729	0.16420002583135165	No Hit
GTATCAACGCAGAGTACATGGGGGT	1722	0.16353524839883607	No Hit
GTTGATACCACTGCTTATGTACTCT	1713	0.1626805345570303	No Hit
ATACCACTGCTTATGTACTCTGCGT	1707	0.1621107253291598	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1663	0.15793212432477605	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1561	0.1482453674509774	No Hit
ATCATTAACTGAATCCATAGGTTAA	1505	0.14292714799085265	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1470	0.13960326082827468	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1425	0.13532969161924588	No Hit
GCGTTGATACCACTGCTTATCATGT	1393	0.13229070907060317	No Hit
GGTATCAACGCAGAGTACATGGGAA	1393	0.13229070907060317	No Hit
CAGTGGTATCAACGCAGAGTACATA	1391	0.13210077266131298	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	1378	0.1308661860009269	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1366	0.12972656754518586	No Hit
CCCATATTCAGACAGGATACCACGT	1357	0.1288718537033801	No Hit
TGATACCACTGCTTATGTACTCTGC	1347	0.12792217165692926	No Hit
TACCACTGCTTATGTACTCTGCGTT	1340	0.12725739422441365	No Hit
GATAAGCAGTGGTATCAACGCAGAG	1307	0.12412344347112586	No Hit
GTACATGGGGGTTAAGCGACTAAGC	1304	0.12383853885719061	No Hit
GGTATCAACGCAGAGTACATGGGAT	1292	0.1226989204014496	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	1267	0.12032471528532249	No Hit
GGATAAGCAGTGGTATCAACGCAGA	1254	0.11909012862493637	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1245	0.1182354147831306	No Hit
ACGCAGAGTACATGATAAGCAGTGG	1229	0.11671592350880926	No Hit
GTATCAACGCAGAGTACATGGGATA	1174	0.11149267225332958	No Hit
GTATCAACGCAGAGTACATGGGAAG	1173	0.11139770404868449	No Hit
CCCCATGTACTCTGCGTTGATACCA	1156	0.10978324456971805	No Hit
CAGTGGTATCAACGCAGAGTACATG	1132	0.10750400765823602	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	1131	0.10740903945359094	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	1130	0.10731407124894585	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	1129	0.10721910304430077	No Hit
GCAGAGTACATAAGCAGTGGTATCA	1119	0.1062694209978499	No Hit
GCGTTGATACCACTGCTTCCCATGT	1119	0.1062694209978499	No Hit
TATCAACGCAGAGTACATGATAAGC	1102	0.10465496151888347	No Hit
GGGATAAGCAGTGGTATCAACGCAG	1090	0.10351534306314246	No Hit
GTGGATGCCCTGGCAGTCAGAGGCG	1059	0.10057132871914483	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	1058	0.10047636051449974	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.496820464508483E-5	0.0
3	0.0	0.0	0.0	9.496820464508483E-5	0.0
4	0.0	0.0	0.0	9.496820464508483E-5	0.0
5	9.496820464508483E-5	0.0	0.0	9.496820464508483E-5	0.0
6	1.8993640929016966E-4	0.0	0.0	9.496820464508483E-5	0.0
7	1.8993640929016966E-4	0.0	0.0	9.496820464508483E-5	0.0
8	1.8993640929016966E-4	0.0	0.0	9.496820464508483E-5	0.0
9	1.8993640929016966E-4	0.0	0.0	9.496820464508483E-5	0.0
10	1.8993640929016966E-4	0.0	0.0	1.8993640929016966E-4	0.0
11	1.8993640929016966E-4	0.0	0.0	1.8993640929016966E-4	0.0
12	1.8993640929016966E-4	0.0	0.0	1.8993640929016966E-4	0.0
13	1.8993640929016966E-4	0.0	0.0	1.8993640929016966E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	25	0.0060290694	19.00095	2
TTATAAG	25	0.0060290694	19.00095	3
AGTTGTA	90	0.0	17.943636	11
CAGTTGT	90	7.2759576E-12	16.888128	10
TACCCGA	35	0.0021702263	16.28498	11
GCAGTTG	105	8.913048E-11	14.47554	9
ATATGCC	40	0.0052758544	14.250712	3
TCGGAAC	40	0.0052758544	14.250712	3
ATGAGTC	40	0.005282511	14.248004	7
AAATCGC	170	0.0	13.968632	17
CCGAATG	130	1.8189894E-12	13.882671	18
TACACAG	55	1.9583743E-4	13.818873	5
CGGAACT	50	0.0014984212	13.300665	4
AGTGGTC	50	0.0015000317	13.298771	12
AGCAGTT	115	3.765308E-10	13.216796	8
CCTACAC	65	5.446669E-5	13.154504	3
AATCGCC	175	0.0	13.026747	18
CGAATGG	150	1.8189894E-12	12.67513	19
TAGGACT	75	1.47675E-5	12.6673	4
TTGTATC	135	2.7284841E-11	12.666098	13
>>END_MODULE