FastQCFastQC Report
Wed 25 May 2016
SRR1295312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052984
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA353133.3536122106318804No Hit
GCTTATGTACTCTGCGTTGATACCA212642.019403903573084No Hit
GTATCAACGCAGAGTACTTTTTTTT186721.773246317133024No Hit
CTTATGTACTCTGCGTTGATACCAC154821.4702977443152032No Hit
GGTATCAACGCAGAGTACTTTTTTT135971.2912826785592184No Hit
TATCAACGCAGAGTACTTTTTTTTT119121.1312612537322504No Hit
GAGTACATAAGCAGTGGTATCAACG96640.9177727296900997No Hit
CCCATGTACTCTGCGTTGATACCAC94840.9006784528539844No Hit
TATGTACTCTGCGTTGATACCACTG76370.7252721788745129No Hit
GCGTTGATACCACTGCTTATGTACT75600.7179596271168414No Hit
CATAAGCAGTGGTATCAACGCAGAG72110.6848157236957066No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71640.6803522180773877No Hit
ACTCTGCGTTGATACCACTGCTTAT66020.6269800870668499No Hit
CATGTACTCTGCGTTGATACCACTG65040.6176732030116318No Hit
ACGCAGAGTACATAAGCAGTGGTAT61570.5847192359997873No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60760.5770268114235354No Hit
CTGCTTATGTACTCTGCGTTGATAC57590.5469218905510436No Hit
ATCATGTACTCTGCGTTGATACCAC55610.5281181860313168No Hit
GTGGTATCAACGCAGAGTACATGGG52650.5000075974563716No Hit
GTACATGATAAGCAGTGGTATCAAC50480.4793994970483882No Hit
GTATCAACGCAGAGTACATAAGCAG46550.44207699262286987No Hit
TATCAACGCAGAGTACATAAGCAGT43540.4134915630246993No Hit
GGTATCAACGCAGAGTACATAAGCA41700.39601741337000373No Hit
AAGCAGTGGTATCAACGCAGAGTAC38300.3637282237906749No Hit
GTACTGGTTCACTATCGGTCAGTCA32680.3103560927801372No Hit
GTACATGGGAAGCAGTGGTATCAAC31880.3027586364085304No Hit
ACATAAGCAGTGGTATCAACGCAGA30020.2850945503445447No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29690.28196059959125686No Hit
GTGGTATCAACGCAGAGTACATAAG29320.2784467760193887No Hit
GCAGTGGTATCAACGCAGAGTACAT28630.2718939698988778No Hit
ATACAGGGTGACAGCCCCGTACACA26290.24967141001192802No Hit
GGATACCACGTGTCCCGCCCTACTC25380.24102930338922526No Hit
GTACTCTGCGTTGATACCACTGCTT24770.2352362429058751No Hit
GGTATCAACGCAGAGTACATGGGGG24260.2303928644689758No Hit
CCACTGCTTATGTACTCTGCGTTGA23570.22384005834846493No Hit
GCAGAGTACTTTTTTTTTTTTTTTT23010.21852183888834018No Hit
GATACCACTGCTTATGTACTCTGCG22800.2165275065907934No Hit
GTTAATGATAGTGTGTCGAAACACA21630.20541622664731846No Hit
GTACATGGGATAAGCAGTGGTATCA21520.20437157639622253No Hit
GCTTATCCCATGTACTCTGCGTTGA20720.19677412002461575No Hit
GAGTACATGATAAGCAGTGGTATCA20320.19297539183881235No Hit
ATACCACTGCTTATGTACTCTGCGT19290.18319366676036863No Hit
GCTTATCATGTACTCTGCGTTGATA18870.17920500216527507No Hit
GTGGTATCAACGCAGAGTACTTTTT18700.17759054268630864No Hit
GTTGATACCACTGCTTATGTACTCT18610.17673582884450287No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18430.17502640116089133No Hit
GTATCAACGCAGAGTACATGGGGGT18310.17388678270515032No Hit
ATCATTAACTGAATCCATAGGTTAA17540.16657423094747878No Hit
CCCATATTCAGACAGGATACCACGT17530.1664792627428337No Hit
ATCAACGCAGAGTACTTTTTTTTTT17110.16249059814774014No Hit
CAGTGGTATCAACGCAGAGTACATA16660.1582170289387113No Hit
GCGTTGATACCACTGCTTATCATGT16240.15422836434361775No Hit
ACTTAGATGTTTCAGTTCCCCCGGT16090.1528038412739415No Hit
GATAAGCAGTGGTATCAACGCAGAG15700.14910008129278318No Hit
GGTTAATGAGGCGAACCGGGGGAAC15560.14777052642775199No Hit
GGATAAGCAGTGGTATCAACGCAGA15550.1476755582231069No Hit
ACGCAGAGTACATGATAAGCAGTGG15260.14492148028839943No Hit
TACCACTGCTTATGTACTCTGCGTT15050.14292714799085265No Hit
GAGTACATGGGAAGCAGTGGTATCA14250.13532969161924588No Hit
TGATACCACTGCTTATGTACTCTGC14230.13513975520995572No Hit
GCAGAGTACATAAGCAGTGGTATCA14170.13456994598208522No Hit
GTATTTAGCCTTGGAGGATGGTCCC14080.13371523214027944No Hit
GGTATCAACGCAGAGTACATGGGAA14020.1331454229124089No Hit
GCCTTGGAGGATGGTCCCCCCATAT13740.13048631318234655No Hit
CCCCATGTACTCTGCGTTGATACCA13700.1301064403637662No Hit
GGTATCAACGCAGAGTACATGGGAT13470.12792217165692926No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG13440.127637267042994No Hit
CAGTGGTATCAACGCAGAGTACATG13230.12564293474544722No Hit
AGCGTACACGGTGGATGCCCTGGCA13130.12469325269899637No Hit
GCCCAGAGCCTGAATCAGTGTGTGT13110.12450331628970622No Hit
CACACACACTGATTCAGGCTCTGGG13060.12402847526648078No Hit
TCTAAGTACCCCGAGGAAAAGAAAT12960.12307879322002993No Hit
GTATCAACGCAGAGTACATGGGAAG12920.1226989204014496No Hit
GGGATAAGCAGTGGTATCAACGCAG12860.12212911117357908No Hit
CTATCGGTCAGTCAGGAGTATTTAG12470.11842535119242079No Hit
TATCAACGCAGAGTACATGATAAGC12450.1182354147831306No Hit
GTATCAACGCAGAGTACATGGGATA12230.11614611428093875No Hit
GCGTTGATACCACTGCTTCCCATGT11990.11386687736945669No Hit
GAGTACATGGGATAAGCAGTGGTAT11930.1132970681415862No Hit
ACGCAGAGTACATGGGAAGCAGTGG11850.11253732250442552No Hit
GTTCACTATCGGTCAGTCAGGAGTA11780.11187254507190993No Hit
GGTATCAACGCAGAGTACATGGGGA11680.11092286302545908No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11650.11063795841152382No Hit
GTACATGGGGGTTAAGCGACTAAGC11630.11044802200223364No Hit
TCATTAACCTATGGATTCAGTTAAT11580.10997318097900823No Hit
GTCAGGAGTATTTAGCCTTGGAGGA11470.10892853072791228No Hit
GTGGATGCCCTGGCAGTCAGAGGCG11430.10854865790933196No Hit
TATTCAGACAGGATACCACGTGTCC11330.10759897586288111No Hit
GGTATCAACGCAGAGTACATGATAA11030.10474992972352856No Hit
ATGATAAGCAGTGGTATCAACGCAG10740.10199585178882112No Hit
CCCCAGTAGCGGCGAGCGAACGGGG10700.10161597897024076No Hit
GTATCAACGCAGAGTACATGATAAG10650.10114113794701535No Hit
TATCAACGCAGAGTACATGGGAAGC10650.10114113794701535No Hit
ATCAACGCAGAGTACATAAGCAGTG10550.1001914559005645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA307.638913E-419.0300221
CTCTAAC307.638913E-419.0300221
ACTGATA250.006043714518.9929718
GTCATAT350.002146810316.3114471
GGTTAGA508.5931606E-515.2240181
TCTTAAG456.722817E-414.7884632
ATCGCCG1500.014.57027319
GAATATA400.005222605514.2725161
CTTATAC602.5287713E-514.2725161
CTATTAA400.005222605514.2725161
TGTAGGA400.005252354314.2603042
AACGGGT400.00526894314.25352919
AAATCGC1650.013.8130717
TCACGTT500.001503171913.2950813
AATCGCC1650.013.23752618
AAACTGT802.0038315E-613.0589086
GTACAAA1403.6379788E-1212.913231
TGCTAAC604.0652108E-412.6758262
TACACTA604.0652108E-412.6758265
ACTATGT604.099577E-412.6631836
Produced by FastQC (version 0.11.5)