FastQCFastQC Report
Wed 25 May 2016
SRR1295311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449903
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG47550.13782996217574814No Hit
GTATCAACGCAGAGTACTTTTTTTT37680.10922046214052974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.010.5830151
TATACCG1002.3978899E-510.451115
GTGGTAT12250.010.0891411
TGGTATC12150.09.8527552
GCGTCAG1856.6211214E-99.2501271
CGTCAGA1752.6690032E-89.2294172
GTATTAA6200.09.0470551
TAGACAG4950.09.0211595
CTAGACT3700.08.9874394
CTAGACA4550.08.9789754
TGGACCG850.00743212228.9421255
TAACGCT850.00743212228.9421254
TACACTG6750.08.7268525
TAGACTC2751.8189894E-128.637285
AACCGTG1107.158917E-48.6350267
TAGACTG4650.08.5815545
TTAGACT3800.08.5009024
CTACACT5600.08.4830444
GTCTTAC3400.08.3885961
TCAGGAC4100.08.3423483
Produced by FastQC (version 0.11.5)