##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295311_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3449903
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.490346830041307	31.0	30.0	33.0	27.0	34.0
2	30.1807679810128	31.0	30.0	34.0	26.0	34.0
3	30.64699674164752	31.0	30.0	34.0	27.0	34.0
4	34.00384155728437	35.0	35.0	37.0	28.0	37.0
5	34.28558426135459	37.0	35.0	37.0	30.0	37.0
6	34.431471261655766	37.0	35.0	37.0	30.0	37.0
7	34.28299172469487	37.0	35.0	37.0	30.0	37.0
8	34.352634262470566	37.0	35.0	37.0	30.0	37.0
9	35.31922143897959	39.0	35.0	39.0	28.0	39.0
10	35.23176796565005	38.0	35.0	39.0	28.0	39.0
11	35.68356269727004	39.0	35.0	39.0	30.0	39.0
12	35.67465230181834	39.0	35.0	39.0	30.0	39.0
13	35.599616279066396	39.0	35.0	39.0	29.0	39.0
14	35.79536902921618	39.0	35.0	41.0	26.0	41.0
15	36.448735225309235	39.0	36.0	41.0	27.0	41.0
16	36.54285120480199	39.0	36.0	41.0	29.0	41.0
17	36.33330908144374	39.0	36.0	41.0	27.0	41.0
18	36.29707183071524	39.0	36.0	41.0	27.0	41.0
19	36.375957816785	39.0	36.0	41.0	27.0	41.0
20	36.371067244499336	39.0	36.0	41.0	27.0	41.0
21	36.360935365429114	39.0	36.0	41.0	27.0	41.0
22	36.08683548493972	39.0	35.0	41.0	27.0	41.0
23	35.766097191718146	39.0	34.0	41.0	26.0	41.0
24	36.110085124132475	39.0	35.0	41.0	27.0	41.0
25	34.83860618689859	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4554903076334824
1101	2	0.5902694714843086
1101	3	0.4827418108804089
1101	4	0.44415932959936555
1101	5	0.29479695058368804
1101	6	0.2487304004667834
1101	7	0.28939263392410197
1101	8	0.27784579619024186
1101	9	0.09969949908612818
1101	10	0.5819349922818304
1101	11	0.504889862464097
1101	12	0.570091583697014
1101	13	0.5341694953079497
1101	14	1.065013095267524
1101	15	0.8244287884130443
1101	16	0.6430732417333473
1101	17	0.9636811369584777
1101	18	0.8760962433502257
1101	19	0.9489130026538959
1101	20	0.97474114216201
1101	21	0.936433943878022
1101	22	0.7560272205702248
1101	23	-1.3114219900169672
1101	24	0.24258512361816287
1101	25	0.598605947565936
1102	1	0.30439734958599374
1102	2	0.3519017452376829
1102	3	0.28318763871298813
1102	4	0.44643746483082936
1102	5	0.335918877846197
1102	6	0.13997292389333893
1102	7	0.07834486408057018
1102	8	0.21374745866555145
1102	9	0.3802762962882227
1102	10	0.8267260667265717
1102	11	0.5362204167068967
1102	12	0.47572353455935
1102	13	0.4532317284203913
1102	14	1.020285474205302
1102	15	0.8350324838282077
1102	16	0.491839118105446
1102	17	0.8132234405643857
1102	18	0.8205729456534527
1102	19	0.8780933374647759
1102	20	0.9663451811451509
1102	21	0.906246476847393
1102	22	0.7728271817968349
1102	23	0.8122696933718316
1102	24	0.8577437000061252
1102	25	0.6642131059544099
1103	1	-0.2864494197350709
1103	2	-1.535761097782796
1103	3	-0.3091948107797826
1103	4	0.0144565053804655
1103	5	-0.11100940217474431
1103	6	0.006126570311081991
1103	7	0.027705611719923695
1103	8	0.19455190606380768
1103	9	0.8076993774162702
1103	10	0.7992525287174033
1103	11	0.5227248013846975
1103	12	0.498349471769842
1103	13	0.27360116773403576
1103	14	-0.8722169497389487
1103	15	-0.032452246042268484
1103	16	0.03005015374102271
1103	17	0.4960230386355846
1103	18	0.6580024930304162
1103	19	0.6678951134894504
1103	20	0.6352894922846488
1103	21	0.7536756214324356
1103	22	0.17354443912908835
1103	23	1.105283231496088
1103	24	0.8897997154157977
1103	25	0.7087837944984301
1104	1	0.1441366055165183
1104	2	0.24445811164427056
1104	3	-0.13979369681374365
1104	4	-0.10726546945504367
1104	5	0.0241295214934496
1104	6	0.12304367066057154
1104	7	-0.022732811670643116
1104	8	0.17546963516264924
1104	9	-1.922685547841489
1104	10	-0.47623461594394456
1104	11	-0.31074621263469027
1104	12	0.16922923547621593
1104	13	0.23346520635539747
1104	14	-1.4390141975768884
1104	15	-0.3220762711632119
1104	16	-0.008800863508298562
1104	17	0.33593385693518485
1104	18	0.5154897139038042
1104	19	0.5137425324415688
1104	20	0.6355049727652897
1104	21	0.7048056691193949
1104	22	0.8183046796688132
1104	23	1.1942105515177133
1104	24	1.0242605560346405
1104	25	0.6733473489918538
1105	1	-0.1131733629366316
1105	2	0.06007624518345267
1105	3	-1.2717135045454455
1105	4	-0.4112411707464858
1105	5	-0.3144604717978865
1105	6	-0.12103997055717741
1105	7	-0.09651285420064681
1105	8	-0.007538260479819314
1105	9	0.4386627116188677
1105	10	0.5476813877289146
1105	11	-0.10326115510558509
1105	12	-0.06721655831403694
1105	13	-0.17328860439638305
1105	14	0.6830074412559668
1105	15	0.3903468251308624
1105	16	-0.12281071169079638
1105	17	0.309776852378036
1105	18	0.21890533533981937
1105	19	0.2187450255836012
1105	20	-0.3357580503307105
1105	21	0.19252034059284995
1105	22	0.3229240785192289
1105	23	0.42693540992046763
1105	24	0.3374512412800712
1105	25	0.593231951169173
1106	1	-1.3550262650539615
1106	2	-0.9459777079965015
1106	3	-0.9159840180742371
1106	4	-1.2483264217768735
1106	5	-0.7327118971102848
1106	6	-0.6093198615357238
1106	7	-0.5325572868644741
1106	8	-0.5319903093378642
1106	9	-0.17196176215908565
1106	10	-1.6741237608353359
1106	11	-1.0015492074276722
1106	12	-0.6767995324569966
1106	13	-0.7290493955727655
1106	14	-0.017142475648206812
1106	15	-0.6830302010592462
1106	16	-1.2037816521015543
1106	17	-0.49669934424999695
1106	18	-0.4004809654369197
1106	19	-0.2617306410848741
1106	20	-0.03571763260886485
1106	21	-0.05160917560184686
1106	22	-0.1300328851228585
1106	23	-0.38278534866100244
1106	24	-0.5466057442172811
1106	25	-1.2877433039416744
1107	1	0.10535672196335355
1107	2	0.18347268681793594
1107	3	0.09154612052562427
1107	4	0.21206212379828315
1107	5	-0.023443847609421198
1107	6	0.054808926437814875
1107	7	0.0692686437999015
1107	8	-0.062072688356153094
1107	9	0.4455296932456534
1107	10	0.0036781265253367224
1107	11	0.03200423288486576
1107	12	0.01625253626154688
1107	13	0.12336811453557317
1107	14	-1.1015930890330807
1107	15	-0.4466905256893412
1107	16	-0.13306111332165926
1107	17	0.14966349879193785
1107	18	0.4581830534504263
1107	19	0.3851055131570078
1107	20	0.3858577667070122
1107	21	0.5097975032197937
1107	22	-0.09134079631251524
1107	23	0.6608027973014856
1107	24	0.5088066453872813
1107	25	0.1347063771142345
1108	1	-0.13571941075034744
1108	2	0.2022663389081636
1108	3	-0.08688211735859852
1108	4	-0.3096367028322362
1108	5	-0.38323911166860825
1108	6	-0.2696144616013356
1108	7	-0.10994392573307721
1108	8	-0.23021806831308567
1108	9	0.2799973042739481
1108	10	0.31326183789180817
1108	11	0.04583426529604395
1108	12	-0.013575048212857155
1108	13	-0.17628381945441163
1108	14	0.19795272015556264
1108	15	-0.021057658507743326
1108	16	-0.15592805829850676
1108	17	0.37151324694811905
1108	18	0.2894184608850594
1108	19	0.2105748496047184
1108	20	0.3077587944709279
1108	21	0.23215158729047403
1108	22	0.18377870753945302
1108	23	0.08379330603603563
1108	24	0.16177173797743905
1108	25	-0.8518549945601706
1109	1	-0.7117744599798783
1109	2	-0.6173949039019391
1109	3	-0.6642991632614219
1109	4	-0.4447892852305273
1109	5	-0.6806178069335402
1109	6	-0.9603063320523617
1109	7	-0.7566470451623033
1109	8	-1.0780990905087364
1109	9	-0.7280345041165575
1109	10	-0.9162537158743262
1109	11	-1.0357507181721033
1109	12	-0.9561175504530226
1109	13	-1.0157956859199047
1109	14	-0.3083148775545155
1109	15	-1.1419653987211476
1109	16	-1.155551058920743
1109	17	-1.1428050701244885
1109	18	-1.7145542666464664
1109	19	-1.7430915066237702
1109	20	-1.5589859562092556
1109	21	-1.413071867195086
1109	22	-1.4858104837894217
1109	23	-0.9827300710433278
1109	24	-1.9097737561276205
1109	25	-0.9916288356022633
1110	1	0.10855622587812874
1110	2	0.21659997930736807
1110	3	0.08922235529576028
1110	4	0.33335521653646794
1110	5	0.2685484727705898
1110	6	0.20796461727005777
1110	7	0.10987182847119925
1110	8	0.11445669121732749
1110	9	0.4259450541460552
1110	10	0.2352580420683381
1110	11	0.22933767673828243
1110	12	0.22986655485291152
1110	13	0.2229312649761539
1110	14	-2.548581089553302
1110	15	-0.6545706834014098
1110	16	-0.11958582444604815
1110	17	-0.27845446251926376
1110	18	-2.6955763685522385
1110	19	-0.3317235839202226
1110	20	-1.9311127337479235
1110	21	-0.21852039766925913
1110	22	-1.7323583724366216
1110	23	-0.5368858709772439
1110	24	0.252680486720088
1110	25	0.0891292660399543
1111	1	-0.1166463042552941
1111	2	-1.3568170254978291
1111	3	-0.3815684565587638
1111	4	0.10231816865281473
1111	5	0.06512615177248193
1111	6	0.11491214052860244
1111	7	0.004078732922351946
1111	8	0.0320587788582003
1111	9	-1.714892619845834
1111	10	-0.30145905095932335
1111	11	-0.20515903716916029
1111	12	-0.0016074330040822815
1111	13	0.08961523452076392
1111	14	0.9796355839377426
1111	15	0.3580155738512474
1111	16	0.3543986058194122
1111	17	0.008258751638912543
1111	18	0.16724910151677364
1111	19	-0.0654946398754177
1111	20	0.2919509131857936
1111	21	-0.5512688281549174
1111	22	-0.18664861626282914
1111	23	0.02495474698933009
1111	24	-0.4537095263887494
1111	25	-1.8049628111827047
1112	1	0.20405544372563966
1112	2	0.39111362915556214
1112	3	0.010274928909918657
1112	4	0.27168607698273206
1112	5	0.14493741473086175
1112	6	0.19863979157483413
1112	7	0.041716743321238425
1112	8	0.15584467725423679
1112	9	-0.40221813679986695
1112	10	0.42716889057107466
1112	11	0.08180369884221506
1112	12	0.08840573093539916
1112	13	0.07837024225403866
1112	14	0.5446600167282369
1112	15	0.14349460049793805
1112	16	0.20157636511867594
1112	17	-3.5805602743768787
1112	18	-0.9427181991999021
1112	19	-1.1673895522153614
1112	20	-0.14294254152927266
1112	21	-0.559840104378118
1112	22	-1.4246282069525407
1112	23	-1.3719437449621878
1112	24	-0.3024605533953988
1112	25	0.04784327282791878
1113	1	-0.43358377879820154
1113	2	-0.28507507717942815
1113	3	-0.18689686645591408
1113	4	-0.6096746646438973
1113	5	-0.2694798841479127
1113	6	-0.2692058431499049
1113	7	-0.3600601137697552
1113	8	-0.2963406364234231
1113	9	0.12407277235553238
1113	10	0.30357989564105736
1113	11	-0.1700297891001199
1113	12	-0.21658205932384078
1113	13	-0.5805107116017965
1113	14	-1.628968552025647
1113	15	-1.8598220824403597
1113	16	-0.6257973327549422
1113	17	-0.4413988720802067
1113	18	-0.7770587078856863
1113	19	-0.2890335836428619
1113	20	0.17533057029958599
1113	21	-1.7339337035654552
1113	22	-2.1497468619188354
1113	23	-0.8052859520034659
1113	24	-2.4321660096300306
1113	25	-0.7498853366272087
1114	1	-0.040807426594906104
1114	2	0.17366240673066713
1114	3	-0.11030368470271767
1114	4	0.2993095669363086
1114	5	-0.02195982285256548
1114	6	0.11287716113171342
1114	7	0.08433834848226951
1114	8	0.008934864947647725
1114	9	0.3499343143089604
1114	10	0.12083562076139032
1114	11	0.11435287866944321
1114	12	0.1214891337618127
1114	13	0.06120826806755275
1114	14	0.8007620687997417
1114	15	0.2458174359519134
1114	16	0.2736029222543408
1114	17	-0.6421029183472626
1114	18	-0.23450754471896573
1114	19	-0.6635570227104921
1114	20	-1.8356162560392946
1114	21	-0.9782259161669842
1114	22	-1.629856049312707
1114	23	-2.9800031783891114
1114	24	-2.3224880429836645
1114	25	-1.6737723531931934
1115	1	0.13720893343992913
1115	2	0.24441782736576556
1115	3	0.09811229987336034
1115	4	0.226473052330185
1115	5	0.19960355526738027
1115	6	0.11000665828136391
1115	7	0.30134074680999134
1115	8	0.17050722396398754
1115	9	0.3875120654118973
1115	10	0.6096097537519185
1115	11	0.2897181886073241
1115	12	0.1969476746817307
1115	13	0.059851184407705205
1115	14	0.7121028395725588
1115	15	0.25594825716908076
1115	16	0.29541264765513375
1115	17	0.24081064109528683
1115	18	0.4550678854952537
1115	19	-0.2342260690738982
1115	20	0.40733124982281055
1115	21	-0.6334596837290647
1115	22	0.4367766633299155
1115	23	0.5622649554868318
1115	24	0.5646287686181424
1115	25	0.5579689939488688
1116	1	0.06145240967728682
1116	2	0.23266958481528377
1116	3	0.0382799729197707
1116	4	0.3775535114897579
1116	5	0.2699795043407036
1116	6	0.18202095437516874
1116	7	0.16190899883302734
1116	8	0.04486088661082732
1116	9	0.3781708505095054
1116	10	0.23668045200809473
1116	11	0.17683633815858002
1116	12	0.2186701111640872
1116	13	-0.3072893628630595
1116	14	0.6182405861798657
1116	15	0.3351730760175684
1116	16	0.3120930678621505
1116	17	0.4696464086471721
1116	18	0.49000813821892564
1116	19	0.5761877322718618
1116	20	0.4215106756646705
1116	21	-0.11934342932082131
1116	22	-0.038388932457642966
1116	23	-0.9455536318380382
1116	24	-0.4812633492436902
1116	25	-1.0809138771074274
1201	1	0.4676278623837007
1201	2	0.7206837667822832
1201	3	0.6476526394023985
1201	4	0.4719261100882406
1201	5	0.49000564943303715
1201	6	0.37539662586247147
1201	7	0.3725675202646954
1201	8	0.5429614236713718
1201	9	0.7180065579560093
1201	10	1.0294430973690538
1201	11	0.6088651106904592
1201	12	0.6810808515990487
1201	13	0.5743945905541992
1201	14	1.2212081576020637
1201	15	0.8651115988457079
1201	16	0.6393565029419648
1201	17	0.7089110488321282
1201	18	0.7605250210049945
1201	19	0.9781786125222993
1201	20	1.0728444697481478
1201	21	1.0110715108797166
1201	22	1.0434174710211295
1201	23	0.7101966398601647
1201	24	1.079216184334065
1201	25	1.1636046077443893
1202	1	0.4928114233772263
1202	2	0.8502635489389796
1202	3	0.6338271283691803
1202	4	0.5687402515516382
1202	5	0.4351189901587347
1202	6	0.3998256357730341
1202	7	0.34961466035133526
1202	8	0.511399407823788
1202	9	0.9266070472019479
1202	10	0.16904703641236551
1202	11	0.5039713921507385
1202	12	0.4724901408422042
1202	13	0.6160346789303048
1202	14	1.3123710691056658
1202	15	1.044362020057477
1202	16	0.8629730677560659
1202	17	-1.1717163711764798
1202	18	0.3415709469515562
1202	19	0.7678502106730107
1202	20	0.9942608477261317
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2210	23	1.4544141556846384
2210	24	1.157690051421163
2210	25	1.1531196257826082
2211	1	0.050085366520907115
2211	2	-0.6278793645135039
2211	3	-0.1526127560544559
2211	4	0.2001700904214303
2211	5	0.12020582791831913
2211	6	0.10247916901984411
2211	7	0.1705699727203509
2211	8	0.1871349366579409
2211	9	0.6846420948754712
2211	10	0.7240984389758083
2211	11	0.3052815269304716
2211	12	0.0648380043247343
2211	13	0.08648614544295441
2211	14	-0.9789080638642957
2211	15	-0.11363039481180692
2211	16	0.09629922917438449
2211	17	0.2497938188345259
2211	18	0.16993849328748922
2211	19	0.4786086280603925
2211	20	0.5385321842618893
2211	21	0.5388052778093524
2211	22	-0.16893815759524244
2211	23	0.38356799407414144
2211	24	-0.19735358618911647
2211	25	0.37140512977561
2212	1	-0.08258578638384506
2212	2	-1.0674888847283377
2212	3	-0.39991282080026025
2212	4	0.10595550134443954
2212	5	0.10140303461737687
2212	6	0.06393092080249829
2212	7	0.07778045879496176
2212	8	-0.007809815311226487
2212	9	-2.2933495585706325
2212	10	-0.1118300159671719
2212	11	0.014434462663366787
2212	12	-0.015730849455415807
2212	13	-0.016309057196544074
2212	14	0.5490024652692327
2212	15	0.16499802435158983
2212	16	0.2389838790791785
2212	17	0.2629833361351075
2212	18	0.0594414870724691
2212	19	-0.6541305954660217
2212	20	0.21068083437927498
2212	21	0.27381950841044755
2212	22	-1.7924989299253937
2212	23	-0.34022938191359486
2212	24	0.15075485024822655
2212	25	-0.35792599104212286
2213	1	0.06646613143875513
2213	2	0.3238338341567477
2213	3	0.009714759281006735
2213	4	0.1654742211422544
2213	5	0.18225198536958231
2213	6	0.16004110340524846
2213	7	0.09800485911915757
2213	8	0.11778941338378957
2213	9	0.4974307129957083
2213	10	0.6148023239257938
2213	11	-0.08674766427716207
2213	12	0.07133520941712135
2213	13	0.004295198825630564
2213	14	-1.8577227158711835
2213	15	-0.5022031104442419
2213	16	-0.12589508017936168
2213	17	-2.17744991885084
2213	18	-0.9655225581115019
2213	19	-0.744327391921459
2213	20	-0.050792768104130914
2213	21	-0.3586340379797477
2213	22	0.22744835614649617
2213	23	0.7544390749430363
2213	24	0.43698313798855537
2213	25	-1.181916137368333
2214	1	0.1508133406385781
2214	2	0.20339786597024911
2214	3	0.1083561669452564
2214	4	0.39137212055166515
2214	5	0.3325437198369343
2214	6	0.18286647505564702
2214	7	0.3291239296530435
2214	8	0.16212262566538982
2214	9	0.6134832766974441
2214	10	0.6730939664035844
2214	11	0.020841000763738293
2214	12	-0.12658138601223357
2214	13	0.02538351997124977
2214	14	0.9176906813145251
2214	15	0.3606179619982015
2214	16	0.41964899403394895
2214	17	0.2751428683849042
2214	18	0.30372440520708466
2214	19	-0.9647855018559284
2214	20	0.09496339103768747
2214	21	0.43169470988166125
2214	22	0.5128948660761381
2214	23	1.0964795555038904
2214	24	0.7734335031142834
2214	25	0.7893254186052374
2215	1	-0.3360125301579373
2215	2	0.1719720005504719
2215	3	-0.011893830707244035
2215	4	0.2627058907126596
2215	5	0.18801290245598068
2215	6	0.09647891759500027
2215	7	0.11206155192395073
2215	8	-0.007506551901066416
2215	9	0.1942388316184278
2215	10	0.4399711297198863
2215	11	0.14113749818027088
2215	12	-0.00146077925913346
2215	13	-0.1014111426018971
2215	14	0.7542131717003926
2215	15	0.2128057276594575
2215	16	0.2833293725355972
2215	17	0.374501769771058
2215	18	0.22153189181058508
2215	19	-0.59566960322001
2215	20	-1.6888900450504778
2215	21	-2.0507806352745774
2215	22	-0.3777956046108102
2215	23	0.30712477285202056
2215	24	0.4231051170214002
2215	25	-0.3059678083922037
2216	1	-0.2756902911888517
2216	2	-0.4406856487080937
2216	3	-0.3337522403078559
2216	4	0.024925488684388597
2216	5	0.04613167741441515
2216	6	-0.12925785799532719
2216	7	-0.06681810892620632
2216	8	-0.1858024896648942
2216	9	0.17587615674752044
2216	10	-1.8324663414694697
2216	11	-0.6855434896428605
2216	12	-0.38828829348965144
2216	13	-0.33342255291209
2216	14	-0.8449317024650682
2216	15	-0.8839032550463841
2216	16	-0.3451452559689443
2216	17	-0.14507380087393784
2216	18	-0.20619171763878796
2216	19	-1.7480461900714772
2216	20	-1.278963256459754
2216	21	-1.211090961527617
2216	22	-1.0977430591741566
2216	23	-5.125841208574499
2216	24	-2.503543628776775
2216	25	-1.6662396772901786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	14.0
4	21.0
5	53.0
6	78.0
7	360.0
8	154.0
9	471.0
10	1227.0
11	2449.0
12	4445.0
13	7221.0
14	10443.0
15	13330.0
16	16078.0
17	17746.0
18	18673.0
19	19535.0
20	20006.0
21	21347.0
22	22221.0
23	23793.0
24	26327.0
25	29033.0
26	33489.0
27	38605.0
28	45587.0
29	56518.0
30	70278.0
31	87797.0
32	116128.0
33	156826.0
34	213698.0
35	295755.0
36	420788.0
37	627614.0
38	1031795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.053347072171256	21.227171801035173	13.68197955303522	23.037501573758355
2	15.889595630443102	21.35235350735797	40.29974023865574	22.45831062354319
3	18.01761862913577	26.217706597606295	30.42392742398222	25.34074734927571
4	12.570238748679602	17.54299282672417	38.873796564749256	31.012971859846967
5	13.449517080309919	37.49215239192195	35.381120445596245	13.677210082171886
6	30.75533921191449	38.734911553108	17.765105117206808	12.7446441177707
7	26.098782977985636	32.50817518599472	24.000357188727563	17.39268464729208
8	23.275308104973178	37.09989850659707	21.723908945918517	17.900884442511238
9	24.336914215986205	17.17028689011854	22.49811367151728	35.994685222377974
10	14.921405401769508	29.965277818046026	34.1133550511265	20.99996172905797
11	31.739306664068863	25.878847130699807	23.96905363310162	18.412792572129714
12	20.77336898916641	27.552886522141485	31.921982377730725	19.751762110961383
13	26.12992724475574	24.373079312871326	27.17963467838575	22.317358763987183
14	20.893880701843926	23.525447439132677	28.32761377588808	27.25305808313532
15	22.512097158882156	30.960114390537203	24.560415039769037	21.9673734108116
16	21.92350335808649	31.39419447095786	25.77448805272735	20.907814118228305
17	19.9991185881638	30.281048601744498	28.799029983079212	20.920802827012487
18	20.083025546723544	30.178107397954896	29.480912011013626	20.257955044307934
19	20.83902670397401	28.81134017958435	29.774001815500288	20.575631300941353
20	22.260320736856904	28.533287719486438	28.88661141786101	20.319780125795646
21	22.706348012207414	29.08674286009163	27.6361919058474	20.570717221853556
22	21.43496858146769	28.59806922369711	29.50950094213557	20.457461252699634
23	21.741594843644855	28.994205160008267	28.63622802798293	20.627971968363948
24	21.409411150734975	28.101591719579023	29.076222782754925	21.412774346931084
25	22.12833289227498	28.457433226086515	28.284462906992403	21.12977097464611
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1539.0
1	1539.0
2	1961.5
3	2384.0
4	2384.0
5	2384.0
6	3746.0
7	5108.0
8	5108.0
9	5108.0
10	8482.0
11	11856.0
12	11856.0
13	11856.0
14	20287.5
15	28719.0
16	28719.0
17	28719.0
18	47420.5
19	66122.0
20	66122.0
21	66122.0
22	96995.0
23	127868.0
24	127868.0
25	127868.0
26	169061.0
27	210254.0
28	210254.0
29	210254.0
30	255393.5
31	300533.0
32	300533.0
33	300533.0
34	339805.0
35	379077.0
36	379077.0
37	379077.0
38	404135.0
39	429193.0
40	429193.0
41	429193.0
42	425479.0
43	421765.0
44	421765.0
45	421765.0
46	405214.5
47	388664.0
48	388664.0
49	388664.0
50	367635.5
51	346607.0
52	346607.0
53	346607.0
54	313222.5
55	279838.0
56	279838.0
57	279838.0
58	240946.0
59	202054.0
60	202054.0
61	202054.0
62	164867.5
63	127681.0
64	127681.0
65	127681.0
66	98069.0
67	68457.0
68	68457.0
69	68457.0
70	49873.5
71	31290.0
72	31290.0
73	31290.0
74	22175.5
75	13061.0
76	13061.0
77	13061.0
78	9076.5
79	5092.0
80	5092.0
81	5092.0
82	3458.5
83	1825.0
84	1825.0
85	1825.0
86	1244.5
87	664.0
88	664.0
89	664.0
90	427.5
91	191.0
92	191.0
93	191.0
94	116.5
95	42.0
96	42.0
97	42.0
98	30.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07945150921634608
2	0.01689902585666901
3	0.027247142890684174
4	0.03275454411326927
5	0.04058085111378494
6	0.01907300002347892
7	0.021623796379202545
8	0.040667810080457334
9	0.040725782724905596
10	0.023507907323771133
11	0.024319524346046832
12	0.018290369323427355
13	0.027305115535132436
14	0.043508469658422276
15	0.03959531615816445
16	0.02652248483508087
17	0.0258268131017017
18	0.03145015961318333
19	0.020754206712478584
20	0.01907300002347892
21	0.01518883284544522
22	0.02388472951268485
23	0.023189057779305678
24	0.023276016745978075
25	0.10571311715140977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3449903.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.26635926609973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.84648173413468	76.5530226786976
2	7.208086477209666	12.147984094193296
3	1.0238970961770284	2.588402416739092
4	0.32141091290872636	1.083365098368473
5	0.14997688354512606	0.6319002975211796
6	0.09328558990249484	0.47165022198442064
7	0.07040156808086948	0.4152738680159528
8	0.048197766533434945	0.3249160248424022
9	0.036012908399345574	0.27312090096567554
>10	0.1827979145463063	2.7899138991403376
>50	0.011574287616675508	0.6725701727732464
>100	0.007048409818896053	1.0671292232319973
>500	4.832631601090418E-4	0.2708649748115368
>1k	3.4518796649168324E-4	0.7098861287148239
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4755	0.13782996217574814	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3768	0.10922046214052974	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.8986322224132098E-5	0.0	0.0	0.0
5	2.8986322224132098E-5	2.8986322224132098E-5	0.0	0.0	0.0
6	2.8986322224132098E-5	2.8986322224132098E-5	0.0	0.0	0.0
7	2.8986322224132098E-5	2.8986322224132098E-5	0.0	0.0	0.0
8	5.7972644448264196E-5	2.8986322224132098E-5	0.0	2.8986322224132098E-5	0.0
9	5.7972644448264196E-5	2.8986322224132098E-5	0.0	5.7972644448264196E-5	0.0
10	5.7972644448264196E-5	2.8986322224132098E-5	0.0	5.7972644448264196E-5	0.0
11	5.7972644448264196E-5	2.8986322224132098E-5	0.0	5.7972644448264196E-5	0.0
12	5.7972644448264196E-5	2.8986322224132098E-5	0.0	5.7972644448264196E-5	0.0
13	5.7972644448264196E-5	2.8986322224132098E-5	0.0	5.7972644448264196E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2695	0.0	10.583015	1
TATACCG	100	2.3978899E-5	10.45111	5
GTGGTAT	1225	0.0	10.089141	1
TGGTATC	1215	0.0	9.852755	2
GCGTCAG	185	6.6211214E-9	9.250127	1
CGTCAGA	175	2.6690032E-8	9.229417	2
GTATTAA	620	0.0	9.047055	1
TAGACAG	495	0.0	9.021159	5
CTAGACT	370	0.0	8.987439	4
CTAGACA	455	0.0	8.978975	4
TGGACCG	85	0.0074321222	8.942125	5
TAACGCT	85	0.0074321222	8.942125	4
TACACTG	675	0.0	8.726852	5
TAGACTC	275	1.8189894E-12	8.63728	5
AACCGTG	110	7.158917E-4	8.635026	7
TAGACTG	465	0.0	8.581554	5
TTAGACT	380	0.0	8.500902	4
CTACACT	560	0.0	8.483044	4
GTCTTAC	340	0.0	8.388596	1
TCAGGAC	410	0.0	8.342348	3
>>END_MODULE