FastQCFastQC Report
Wed 25 May 2016
SRR1295311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449903
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT108630.314878418320747No Hit
GGTATCAACGCAGAGTACTTTTTTT89080.2582101583725687No Hit
TATCAACGCAGAGTACTTTTTTTTT63740.184758817856618No Hit
GTGGTATCAACGCAGAGTACATGGG53790.15591742724360658No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41170.11933668859675185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2250.015.6333533
CAGCGTC2700.013.3798972
TACACCG655.419911E-513.1632185
ACCGTCT655.4762437E-513.1487198
GCAGCGT2700.012.6911591
CCGTGTA550.003053419512.0995242
CCGGTCG550.003078392212.0863719
GGTATCA35550.010.9775851
GTGGTAT16400.010.73718551
TGGTATC16000.010.5168632
ACCATCG750.002657552210.1293848
ATAGGAC2750.010.0253193
GTCTAAG2750.09.691431
GCGTCAG3700.09.5067694
CGGGGCT1054.463094E-49.0540664
CAGCTCG950.00183087468.9966239
TAGACGG850.00739526478.9475475
GTATTAG5600.08.8384861
ACCGTGT1201.7723073E-48.7049398
TACACTG7000.08.6919035
Produced by FastQC (version 0.11.5)