FastQCFastQC Report
Wed 25 May 2016
SRR1295310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5666787
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG70640.12465617641884194No Hit
ATTCCATTCCATTCCATTCCATTCC60320.1064447984369273No Hit
GTATCAACGCAGAGTACTTTTTTTT59520.1050330637096471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC1001.9278577E-611.4022093
CTACGCT950.00182179289.0017454
GTCCTAG6350.08.9841471
GTGTTAG8150.08.9832281
TAGACAG7850.08.95714955
GTACTAG4400.08.8599341
CTTAGAC3400.08.6634443
GGTATCA49750.08.4475051
TTAGACT5400.08.4460814
TAATACG1252.7398928E-48.361624
CTAGTAC3650.08.3303813
GTCTTAG5700.08.1737351
GTATTAG8500.08.1658851
CGAATTA1050.0045239338.141215
TCAGTAC5050.08.0906773
AATACTG10150.08.0508215
GTCCTAA5200.08.0454181
GTTCTAT7150.07.9789271
TATTCCG2158.628376E-87.95502955
AGGACCG1200.00168880527.9182015
Produced by FastQC (version 0.11.5)