##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295310_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5666787
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.800009952729827	31.0	30.0	33.0	25.0	34.0
2	29.785489378725547	31.0	30.0	33.0	25.0	34.0
3	30.17056896615313	31.0	30.0	34.0	26.0	34.0
4	33.564827123376965	35.0	33.0	37.0	28.0	37.0
5	33.74794623478878	35.0	35.0	37.0	28.0	37.0
6	33.91689594120972	35.0	35.0	37.0	28.0	37.0
7	33.828804223627955	35.0	35.0	37.0	28.0	37.0
8	33.837080341999794	35.0	35.0	37.0	28.0	37.0
9	34.837646094691756	38.0	34.0	39.0	27.0	39.0
10	34.72258653801528	38.0	34.0	39.0	27.0	39.0
11	35.125296751051344	38.0	35.0	39.0	27.0	39.0
12	35.11576489463959	38.0	35.0	39.0	27.0	39.0
13	35.025612044356	38.0	35.0	39.0	27.0	39.0
14	35.15209800544824	38.0	33.0	40.0	24.0	41.0
15	35.77235018715191	39.0	34.0	41.0	27.0	41.0
16	35.880390951698026	39.0	34.0	41.0	27.0	41.0
17	35.688057094787574	39.0	34.0	40.0	27.0	41.0
18	35.6589908884876	39.0	34.0	40.0	27.0	41.0
19	35.70845383812732	39.0	34.0	41.0	26.0	41.0
20	35.672270194732924	39.0	34.0	41.0	26.0	41.0
21	35.661799005327005	39.0	34.0	40.0	26.0	41.0
22	35.39556136484396	39.0	34.0	40.0	25.0	41.0
23	35.061809452163985	38.0	33.0	40.0	24.0	41.0
24	35.388062053505806	39.0	34.0	40.0	25.0	41.0
25	33.9476306414905	38.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14926451239355032
1101	2	0.4644736435232062
1101	3	0.22078594330690393
1101	4	0.22211352512324822
1101	5	0.1031118926811061
1101	6	0.15224808270395584
1101	7	0.017426089305601522
1101	8	0.13316573916876706
1101	9	-0.07828853941786207
1101	10	0.2890077365535788
1101	11	0.2536537755008865
1101	12	0.32111704168987387
1101	13	0.24471681715591131
1101	14	0.7287892347676177
1101	15	0.48004846590882977
1101	16	0.3831887658006039
1101	17	0.6293679713949132
1101	18	0.6145456047374935
1101	19	0.5862664036039575
1101	20	0.7311375552092088
1101	21	0.7095883807496577
1101	22	0.42335381109921144
1101	23	-1.8319542163490112
1101	24	-0.1559921929429322
1101	25	0.31739909197942495
1102	1	0.20408229602983496
1102	2	0.27397620267130307
1102	3	0.3266926284407674
1102	4	0.3963923026934779
1102	5	0.16170781085846642
1102	6	0.10263936100498228
1102	7	0.13641258898797304
1102	8	0.17922493710748455
1102	9	0.4481477190987917
1102	10	0.9035725397745935
1102	11	0.6513291266012544
1102	12	0.4871843235839606
1102	13	0.4429722614531002
1102	14	1.0747786269245552
1102	15	0.7054175890408203
1102	16	0.4400574991053645
1102	17	0.7899722323375897
1102	18	0.7663835269001922
1102	19	0.8136581419025148
1102	20	0.8661789220397793
1102	21	0.878139032422169
1102	22	0.6770953743645762
1102	23	0.7344455367861187
1102	24	0.8094622028795442
1102	25	0.6172546855620027
1103	1	-0.4134228403744764
1103	2	-1.5281903149970972
1103	3	-0.36726188149456007
1103	4	-0.020404273880501478
1103	5	-0.2669287830281064
1103	6	-0.17402052984015626
1103	7	-0.11992825795917383
1103	8	0.027891566251888378
1103	9	0.6826151408354306
1103	10	0.7076920909211424
1103	11	0.49093888640209116
1103	12	0.26783915677789594
1103	13	0.06935904698678286
1103	14	-1.1239930427242584
1103	15	-0.33208093297493235
1103	16	-0.1826474367730242
1103	17	0.28892422697277453
1103	18	0.3626984922161043
1103	19	0.41275398639980665
1103	20	0.43379049512549983
1103	21	0.5780134637527325
1103	22	0.1179792765745944
1103	23	0.9662229181078033
1103	24	0.7083852704723981
1103	25	0.5328582288847201
1104	1	-0.13423906921973838
1104	2	-0.026258132162404024
1104	3	-0.4425499974942859
1104	4	-0.3631173006742401
1104	5	-0.3355756666380145
1104	6	-0.2327798217986441
1104	7	-0.21150109072804923
1104	8	-0.13294387263062646
1104	9	-2.4761178441544516
1104	10	-0.7851376523854725
1104	11	-0.7093767491389897
1104	12	-0.16699291647147874
1104	13	8.565001031897168E-4
1104	14	-1.804918453002081
1104	15	-0.6703197015611764
1104	16	-0.2974137359895792
1104	17	0.08133904501443823
1104	18	0.2516609368396914
1104	19	0.27698313241742767
1104	20	0.4122509959363754
1104	21	0.4131103917394938
1104	22	0.5938358863462057
1104	23	0.9951670145674072
1104	24	0.6399908484152235
1104	25	0.4135934500570855
1105	1	-0.3646280327174303
1105	2	-0.24685514109443218
1105	3	-1.5770916288314432
1105	4	-0.6854943476070687
1105	5	-0.6218420998239935
1105	6	-0.26939661835354656
1105	7	-0.2927615238280268
1105	8	-0.2673793468711594
1105	9	0.058751398321398085
1105	10	0.25250234275392813
1105	11	-0.41780935346699266
1105	12	-0.3747637957444141
1105	13	-0.3816039651810428
1105	14	0.42559231021874666
1105	15	0.1667687111931997
1105	16	-0.3122107167229302
1105	17	0.23550862157423325
1105	18	0.13533213242631348
1105	19	0.10651473314508308
1105	20	-0.5181900197495466
1105	21	-0.14083645719274074
1105	22	-0.011410671564256347
1105	23	0.17650900208896303
1105	24	0.17747097111693932
1105	25	0.3003528057786582
1106	1	-1.8096455687637487
1106	2	-1.2238561211451575
1106	3	-1.2778454878620842
1106	4	-1.5448533303480758
1106	5	-1.085743753611041
1106	6	-1.01546926250969
1106	7	-0.8213174281481486
1106	8	-0.9662534537238798
1106	9	-0.5380942495154386
1106	10	-2.136099016758976
1106	11	-1.4279790789621671
1106	12	-1.199002443921735
1106	13	-1.2344553043031894
1106	14	-0.5505440210513441
1106	15	-1.3283961723076416
1106	16	-1.7375610330708753
1106	17	-0.983869389298242
1106	18	-0.8876795751016928
1106	19	-0.7474022169422412
1106	20	-0.6038818617658919
1106	21	-0.6386935348549656
1106	22	-0.5520549416336067
1106	23	-0.927103057713552
1106	24	-1.0526143852162946
1106	25	-1.6934005522220943
1107	1	-0.12140105465716644
1107	2	0.06490186772510143
1107	3	-0.032779207577281255
1107	4	0.052664530700262446
1107	5	-0.1670033220297924
1107	6	-0.06749946833700449
1107	7	-0.19609246399175362
1107	8	-0.16802522111713358
1107	9	0.39923242541348003
1107	10	-0.0825945454217063
1107	11	-0.10755983034123062
1107	12	-0.17075671477785193
1107	13	-0.009176939257379502
1107	14	-1.350363162748998
1107	15	-0.6609320159080383
1107	16	-0.3690111193385235
1107	17	-0.14890304583149572
1107	18	0.21536190627178797
1107	19	0.08717440331000148
1107	20	0.22621995835998376
1107	21	0.36209362706090786
1107	22	-0.3548376200618364
1107	23	0.4667282586578665
1107	24	0.29933623566776646
1107	25	-0.09446802324345072
1108	1	-0.10571545537957405
1108	2	0.10073669332162538
1108	3	-0.09825390032411718
1108	4	-0.3749682041972733
1108	5	-0.33429061713057706
1108	6	-0.2780403563483915
1108	7	-0.19473549933160683
1108	8	-0.2834985363902618
1108	9	0.2731452160221721
1108	10	0.28081133486052323
1108	11	0.04476779412882337
1108	12	-0.07191654764437772
1108	13	-0.2849381465365681
1108	14	0.035345394423814014
1108	15	-0.028175078797367803
1108	16	-0.13236297204066716
1108	17	0.2434948297979318
1108	18	0.28082068154460416
1108	19	0.27823842522713704
1108	20	0.40655055205115787
1108	21	0.3646923077471911
1108	22	0.3210806192206519
1108	23	-0.01262310427584623
1108	24	0.10682372361487324
1108	25	-0.5556319885311112
1109	1	-0.8548339869029853
1109	2	-0.6670739593496506
1109	3	-0.8142145086621007
1109	4	-0.7164956081998426
1109	5	-1.0218527123229393
1109	6	-1.2735257409150051
1109	7	-0.957872861849026
1109	8	-1.369430642528208
1109	9	-0.9481825790280922
1109	10	-1.124806987210178
1109	11	-1.1848267823453256
1109	12	-1.159701504677983
1109	13	-1.186795986876426
1109	14	-0.7027984907524427
1109	15	-1.3223837373541087
1109	16	-1.4293529858759797
1109	17	-1.4873159237017006
1109	18	-2.149564202102596
1109	19	-2.404059546459848
1109	20	-2.0268118863733875
1109	21	-1.7041778741908118
1109	22	-1.8110332107910594
1109	23	-1.2141692082995235
1109	24	-2.176327443031525
1109	25	-1.1811214997838704
1110	1	0.10132854216675113
1110	2	0.2075116596455402
1110	3	0.02897390409191658
1110	4	0.32455739804765926
1110	5	0.18033866332292803
1110	6	0.14761935907876733
1110	7	0.11581699558286829
1110	8	0.1269329975493676
1110	9	0.4227416062787839
1110	10	0.27092791925088733
1110	11	0.22438917988980478
1110	12	0.27007800959567874
1110	13	0.2261813977101994
1110	14	-2.3393728423836677
1110	15	-0.6177349794502547
1110	16	-0.11955705875433864
1110	17	-0.2173357069540529
1110	18	-2.8661488385738494
1110	19	-0.5182544130275772
1110	20	-2.0229044131655485
1110	21	-0.2404600219758919
1110	22	-1.7687838131231786
1110	23	-0.45902949482474753
1110	24	0.09428227333362571
1110	25	0.17526715277952576
1111	1	-0.12426373864507667
1111	2	-1.2166331099441763
1111	3	-0.29904067636143594
1111	4	-0.014728981756775283
1111	5	-0.08507856605785236
1111	6	0.06770258978701094
1111	7	-0.04897132218821554
1111	8	0.07373263613981607
1111	9	-1.7491127870333614
1111	10	-0.31497912181716714
1111	11	-0.10078977954214707
1111	12	-0.11237344389004278
1111	13	0.08942757618923736
1111	14	0.9592161065943401
1111	15	0.36396621612267666
1111	16	0.3466725428242654
1111	17	0.0032636189485941713
1111	18	0.15705812294078214
1111	19	-0.05844290183543421
1111	20	0.37047778731835734
1111	21	-0.4929836280526487
1111	22	-0.2185456013923215
1111	23	-0.054234471488172176
1111	24	-0.42788455485610655
1111	25	-1.9017034409870348
1112	1	0.303675924117222
1112	2	0.4614921714475777
1112	3	0.07565215472619968
1112	4	0.3345790161461366
1112	5	0.2511662813382358
1112	6	0.2111185465001455
1112	7	0.19342945272063616
1112	8	0.11020310383717202
1112	9	-0.2852463396417022
1112	10	0.5460905140401238
1112	11	0.18686151943414586
1112	12	0.1300261111487231
1112	13	0.16387353518069148
1112	14	0.6064631718918889
1112	15	0.28250542383079846
1112	16	0.34494144058673015
1112	17	-3.5751201813377804
1112	18	-0.9686455807304668
1112	19	-1.2224056751430368
1112	20	-0.07311657480215672
1112	21	-0.5124411598805096
1112	22	-1.6982035986160184
1112	23	-1.3950033314405843
1112	24	-0.34952629485937337
1112	25	0.18779341720203746
1113	1	-0.17994689131929675
1113	2	0.015403382778469421
1113	3	-0.07882812112926985
1113	4	-0.40154940491176916
1113	5	-0.08819607568427301
1113	6	-0.12678930056554094
1113	7	-0.1870577797058388
1113	8	-0.12991468339914292
1113	9	0.3577608443599303
1113	10	0.4648863634663698
1113	11	-0.008236674135993383
1113	12	-0.04924302891571131
1113	13	-0.38694358505157567
1113	14	-1.4292313710994193
1113	15	-1.6314303641040127
1113	16	-0.39904149237992925
1113	17	-0.22892773292439728
1113	18	-0.5896020707253129
1113	19	-0.09336093953830016
1113	20	0.34853960942987783
1113	21	-1.5992247932530432
1113	22	-2.159589417613276
1113	23	-0.7113488831093875
1113	24	-2.447064558925547
1113	25	-0.522053438425651
1114	1	0.297294387090254
1114	2	0.40714526656660865
1114	3	0.13418906988628265
1114	4	0.4378369444933625
1114	5	0.21683736698948763
1114	6	0.31560593470371145
1114	7	0.3782598552896488
1114	8	0.3176290657646277
1114	9	0.6826194441262317
1114	10	0.5458780766833868
1114	11	0.47592944848006624
1114	12	0.4338895846520856
1114	13	0.3890254307183696
1114	14	1.2228218086419602
1114	15	0.737649943496443
1114	16	0.660185448753829
1114	17	-0.15584087135578528
1114	18	0.10581727227795312
1114	19	-0.25873383814349893
1114	20	-1.4239706194378883
1114	21	-0.5272196499139667
1114	22	-1.3305100179577565
1114	23	-2.8207236582481414
1114	24	-1.8475217624410547
1114	25	-1.2652093972547362
1115	1	0.5216147431693798
1115	2	0.5860625302495492
1115	3	0.4342375883464271
1115	4	0.637877052785754
1115	5	0.6246659090320037
1115	6	0.47933269113725174
1115	7	0.6423943584748883
1115	8	0.681438284370671
1115	9	0.8679996140642174
1115	10	1.1339251727599162
1115	11	0.6836692928891921
1115	12	0.6050712506137614
1115	13	0.5375017598970047
1115	14	1.285252044287013
1115	15	0.8185716900751245
1115	16	0.8079753533330916
1115	17	0.7558438512503898
1115	18	0.9658739093698046
1115	19	0.41494662970056595
1115	20	0.9663755393481921
1115	21	-0.14767191230918542
1115	22	0.9320136715289067
1115	23	1.0206966860555156
1115	24	1.0950212288274557
1115	25	1.2491844245725474
1116	1	0.7096489852503041
1116	2	0.8821917673025794
1116	3	0.7070135892373379
1116	4	0.9331292159723716
1116	5	0.9389861802767214
1116	6	0.7211864711569547
1116	7	0.7462675780350949
1116	8	0.728166569217592
1116	9	0.8530712533514162
1116	10	0.8976203686983268
1116	11	0.7727205590835169
1116	12	0.8385903359023743
1116	13	0.3249789388647599
1116	14	1.3442532069790474
1116	15	1.0345866684531941
1116	16	0.9937426763803288
1116	17	1.1238539064103108
1116	18	1.1608684605662347
1116	19	1.3075637378698701
1116	20	1.1659551260840857
1116	21	0.588369922024448
1116	22	0.6494102932510231
1116	23	-0.36180431891368414
1116	24	0.153722179220118
1116	25	-0.3404916159978768
1201	1	0.24775758575570705
1201	2	0.5087362803567252
1201	3	0.46144676570871823
1201	4	0.372417497400896
1201	5	0.3134904158486478
1201	6	0.18334134338297048
1201	7	0.16583093322289244
1201	8	0.2528482982718856
1201	9	0.48030619024698495
1201	10	0.8153408172115633
1201	11	0.45132898549870504
1201	12	0.5099471849405433
1201	13	0.3640483546733435
1201	14	0.9686060252023125
1201	15	0.651650136771579
1201	16	0.34873314723171944
1201	17	0.46401538770673767
1201	18	0.3530641514313828
1201	19	0.6871308478713232
1201	20	0.8096545375432243
1201	21	0.7533388195266397
1201	22	0.7414859721935159
1201	23	0.28688270240834157
1201	24	0.7572785562138762
1201	25	0.871753994441697
1202	1	0.23220673384638246
1202	2	0.6308529120451603
1202	3	0.46648956229310556
1202	4	0.3897853069126782
1202	5	0.16878274142903393
1202	6	0.17260997849349025
1202	7	0.08403847101887152
1202	8	0.21356188157822942
1202	9	0.7497459159787425
1202	10	-0.045443487911967395
1202	11	0.2075704864033341
1202	12	0.23601731035397222
1202	13	0.2754815992715862
1202	14	0.8896108151198305
1202	15	0.6501870731250179
1202	16	0.46943263631174403
1202	17	-1.565320207797967
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2210	15	-0.08239774069502204
2210	16	0.17039982850337765
2210	17	0.6930947504115466
2210	18	0.6258492988197659
2210	19	0.8700620796294203
2210	20	0.9606576607169472
2210	21	1.0340690838499143
2210	22	1.195681319417865
2210	23	1.5450239696336325
2210	24	1.225758526820286
2210	25	1.1880384484058197
2211	1	0.07114481128965267
2211	2	-0.6213017533598695
2211	3	-0.07109467643963896
2211	4	0.20986445841409562
2211	5	0.2411484487929485
2211	6	0.1429950894938088
2211	7	0.23801504661587103
2211	8	0.25942868704179034
2211	9	0.7421343383613674
2211	10	0.8223813403661566
2211	11	0.42303619166087003
2211	12	0.29683126162372275
2211	13	0.11440423772700825
2211	14	-0.8951584837259148
2211	15	0.00535465896611953
2211	16	0.26419642854164493
2211	17	0.2536689485512653
2211	18	0.22886996881437938
2211	19	0.6392784200864128
2211	20	0.6953742102546769
2211	21	0.6757953186324173
2211	22	-0.04299281694750334
2211	23	0.46662231425044354
2211	24	-0.1372206840381196
2211	25	0.2892314093854651
2212	1	0.04280142897674466
2212	2	-1.0583559718902578
2212	3	-0.36751586093225086
2212	4	0.09568993233048673
2212	5	0.0495834136874862
2212	6	0.13238194209474585
2212	7	0.11287201992176676
2212	8	0.045417888105617976
2212	9	-2.3741099039506537
2212	10	-0.05142177908145129
2212	11	0.05062309300407719
2212	12	-0.03236147294874314
2212	13	0.060831874421445775
2212	14	0.6753423266533147
2212	15	0.3325682910511176
2212	16	0.37739703301047456
2212	17	0.33875871556701753
2212	18	0.058787388136885
2212	19	-0.6332089005430959
2212	20	0.4076936662442776
2212	21	0.3480922776562849
2212	22	-1.722614384773081
2212	23	-0.3492602410108603
2212	24	0.22965200850583045
2212	25	-0.3917507964522997
2213	1	0.17169806930995435
2213	2	0.42834105052684635
2213	3	0.16916753615817726
2213	4	0.33354182483613926
2213	5	0.3441861871972023
2213	6	0.25026984901079885
2213	7	0.1949335022810459
2213	8	0.20127864925930794
2213	9	0.6639028950694481
2213	10	0.6740287340139233
2213	11	0.05408789945673931
2213	12	0.08450990888596266
2213	13	0.04886604677390238
2213	14	-1.749182133971921
2213	15	-0.37344102788757283
2213	16	-0.04851741541192922
2213	17	-2.157260793491268
2213	18	-0.9412717995526947
2213	19	-0.5620236175980367
2213	20	0.2068887247187874
2213	21	-0.22276761668236134
2213	22	0.3005881621876014
2213	23	0.7737346958719016
2213	24	0.6373357864201381
2213	25	-0.9045664012673171
2214	1	0.4974815799068715
2214	2	0.3744642947200205
2214	3	0.31593119737738107
2214	4	0.6247489676506035
2214	5	0.5924344304058877
2214	6	0.5712823404897875
2214	7	0.4986734266078656
2214	8	0.466144139979221
2214	9	0.8758717734743868
2214	10	0.8742750322984776
2214	11	0.22491881919991386
2214	12	0.18965151954187576
2214	13	0.39070176090458375
2214	14	1.2721293183959403
2214	15	0.9078129205248118
2214	16	0.7006014056302519
2214	17	0.6122315140466341
2214	18	0.6171480012321595
2214	19	-0.6219009824954966
2214	20	0.39776954953152455
2214	21	0.8160309641919881
2214	22	0.9386143632404256
2214	23	1.4756299889154505
2214	24	1.2167017666932196
2214	25	1.231266709410896
2215	1	-0.022006737007270516
2215	2	0.36211194635505706
2215	3	0.2976946525720372
2215	4	0.5485938346620145
2215	5	0.5008132427441723
2215	6	0.39911632290353083
2215	7	0.3993897887115949
2215	8	0.376011668295007
2215	9	0.5038924915137102
2215	10	0.8429261475432099
2215	11	0.5610644527455264
2215	12	0.3911485131922845
2215	13	0.2972141206630923
2215	14	1.25057320987095
2215	15	0.7354383344988236
2215	16	0.697488783733391
2215	17	0.8054052895113202
2215	18	0.6871843434694753
2215	19	0.03452968494519837
2215	20	-1.1899145021760944
2215	21	-1.6274440968784418
2215	22	0.07307539998100054
2215	23	0.7892634246413053
2215	24	0.8976572993235123
2215	25	0.3825509415645598
2216	1	0.22693990842225276
2216	2	-0.10558634368235076
2216	3	0.03113768013271212
2216	4	0.3073890633927405
2216	5	0.45670660448917033
2216	6	0.20555496949729957
2216	7	0.34865960966011755
2216	8	0.20953358333282068
2216	9	0.5060300790068482
2216	10	-1.336985631662074
2216	11	-0.258992352682192
2216	12	0.11700459536844221
2216	13	0.28538287864446943
2216	14	-0.3320854538428506
2216	15	-0.22865551206315615
2216	16	0.22535018665238482
2216	17	0.5594632420026926
2216	18	0.4229274055064991
2216	19	-1.182635349375417
2216	20	-0.7263445135715543
2216	21	-0.6024525011603785
2216	22	-0.47978162626721144
2216	23	-4.2276987254801135
2216	24	-1.8529903405500505
2216	25	-0.8070332800807876
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	25.0
4	45.0
5	68.0
6	120.0
7	438.0
8	272.0
9	727.0
10	1990.0
11	4588.0
12	8950.0
13	14935.0
14	21497.0
15	27825.0
16	32459.0
17	36372.0
18	38833.0
19	40657.0
20	42410.0
21	44359.0
22	47039.0
23	50696.0
24	55077.0
25	60855.0
26	68461.0
27	78934.0
28	91960.0
29	112277.0
30	136526.0
31	167425.0
32	216660.0
33	284078.0
34	375025.0
35	502931.0
36	687984.0
37	973920.0
38	1440369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.69370378736328	21.40981446156732	13.94089338970877	22.955588361360633
2	15.557094605506375	21.326590458984583	40.627953559520726	22.488361375988315
3	17.889432635931268	26.550991171386933	30.328834769936396	25.2307414227454
4	12.49452103085646	17.61895254643421	39.058187490809544	30.82833893189979
5	13.69715734465177	37.37285252578964	35.28643081766836	13.643559311890236
6	30.44763917486107	38.77862805598022	17.798928247054548	12.974804522104154
7	25.629879004139095	32.83032674865785	24.007687622303283	17.53210662489977
8	23.205701843735955	37.12797196106268	21.846105014675107	17.820221180526254
9	24.125351075867695	17.38529462127939	22.5881551283115	35.90119917454142
10	15.080435863112887	29.961441194909945	33.77014250131318	21.18798044066399
11	31.700267227715432	26.104176447954135	23.885886352324036	18.309669972006397
12	20.685276903249854	27.61263231316254	31.839311362650403	19.862779420937205
13	25.943786626620412	24.538090449671333	27.345934982381802	22.172187941326452
14	20.93467753979712	23.407294346685365	28.30830599692194	27.349722116595576
15	22.499382145051158	31.009345497426192	24.4131437165917	22.07812864093095
16	21.935627012847274	31.522984275653865	25.784708167813434	20.756680543685427
17	20.082756862927834	30.439296571960313	28.782623706383465	20.69532285872839
18	20.12491401416055	30.295981926072212	29.4724772059196	20.106626853847636
19	20.85310850732839	28.89347510154835	29.712957135247542	20.54045925587572
20	22.122960105853966	28.630271629368508	28.867391150491205	20.379377114286314
21	22.596527442326558	29.153861671281554	27.84154414963565	20.40806673675624
22	21.273854704152427	28.685390953983426	29.541214482917592	20.49953985894656
23	21.71966538126457	28.91646392519722	28.780645967503943	20.583224726034263
24	21.28275524891474	28.024823079957535	29.0994132229144	21.59300844821333
25	22.127139817831615	28.403732687087047	28.358331035279555	21.110796459801783
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3695.0
1	3695.0
2	4309.0
3	4923.0
4	4923.0
5	4923.0
6	7240.5
7	9558.0
8	9558.0
9	9558.0
10	15412.5
11	21267.0
12	21267.0
13	21267.0
14	36578.0
15	51889.0
16	51889.0
17	51889.0
18	83118.0
19	114347.0
20	114347.0
21	114347.0
22	164189.5
23	214032.0
24	214032.0
25	214032.0
26	280169.0
27	346306.0
28	346306.0
29	346306.0
30	419970.5
31	493635.0
32	493635.0
33	493635.0
34	555471.5
35	617308.0
36	617308.0
37	617308.0
38	661626.0
39	705944.0
40	705944.0
41	705944.0
42	703923.5
43	701903.0
44	701903.0
45	701903.0
46	667436.0
47	632969.0
48	632969.0
49	632969.0
50	595564.5
51	558160.0
52	558160.0
53	558160.0
54	503036.0
55	447912.0
56	447912.0
57	447912.0
58	386077.5
59	324243.0
60	324243.0
61	324243.0
62	267725.0
63	211207.0
64	211207.0
65	211207.0
66	163310.5
67	115414.0
68	115414.0
69	115414.0
70	85171.5
71	54929.0
72	54929.0
73	54929.0
74	39324.5
75	23720.0
76	23720.0
77	23720.0
78	16298.5
79	8877.0
80	8877.0
81	8877.0
82	6055.5
83	3234.0
84	3234.0
85	3234.0
86	2101.5
87	969.0
88	969.0
89	969.0
90	621.0
91	273.0
92	273.0
93	273.0
94	169.0
95	65.0
96	65.0
97	65.0
98	36.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07958654525042144
2	0.01683493662281642
3	0.027722940706964988
4	0.03430515387290894
5	0.04235204181840609
6	0.016393769520541356
7	0.01863489840009868
8	0.03693450980246831
9	0.036299229175192224
10	0.020381920125107932
11	0.021299547697840062
12	0.015405555211445217
13	0.023611263313761396
14	0.038487418002476535
15	0.03576982865246214
16	0.023028922738758312
17	0.022499522216028234
18	0.02841116138651409
19	0.01787609098418557
20	0.015317321790990202
21	0.012423265600065788
22	0.019993693075105876
23	0.01967605276146783
24	0.01927017902737477
25	0.10940944136421574
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5666787.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.51802536063431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.30946093709356	66.90538981784296
2	10.141499029666653	15.722979579530955
3	1.976926804909292	4.597423865972288
4	0.6057608367455731	1.878295356212896
5	0.2634461226914608	1.021091160997872
6	0.15231881200411335	0.7084472119101929
7	0.11041901514696616	0.5991624811521151
8	0.07204961286649449	0.44681149739270426
9	0.05632600905598424	0.3929652898618594
>10	0.281391451196632	3.9174186147650927
>50	0.01866665044872023	0.9809107900023295
>100	0.010592274218017735	1.4668831339885031
>500	7.083195513678195E-4	0.3893992367988509
>1k	3.655784465328354E-4	0.635447129513766
>5k	6.854595870755016E-5	0.3373748340576619
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	7064	0.12465617641884194	No Hit
ATTCCATTCCATTCCATTCCATTCC	6032	0.1064447984369273	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5952	0.1050330637096471	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.7646684091002538E-5	0.0	0.0	0.0
6	0.0	1.7646684091002538E-5	1.7646684091002538E-5	0.0	0.0
7	0.0	1.7646684091002538E-5	1.7646684091002538E-5	0.0	0.0
8	0.0	1.7646684091002538E-5	1.7646684091002538E-5	0.0	0.0
9	0.0	1.7646684091002538E-5	1.7646684091002538E-5	3.5293368182005075E-5	0.0
10	3.5293368182005075E-5	1.7646684091002538E-5	1.7646684091002538E-5	5.2940052273007616E-5	0.0
11	3.5293368182005075E-5	1.7646684091002538E-5	1.7646684091002538E-5	8.823342045501269E-5	0.0
12	3.5293368182005075E-5	1.7646684091002538E-5	1.7646684091002538E-5	8.823342045501269E-5	0.0
13	3.5293368182005075E-5	1.7646684091002538E-5	1.7646684091002538E-5	1.0588010454601523E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTACGC	100	1.9278577E-6	11.402209	3
CTACGCT	95	0.0018217928	9.001745	4
GTCCTAG	635	0.0	8.984147	1
GTGTTAG	815	0.0	8.983228	1
TAGACAG	785	0.0	8.9571495	5
GTACTAG	440	0.0	8.859934	1
CTTAGAC	340	0.0	8.663444	3
GGTATCA	4975	0.0	8.447505	1
TTAGACT	540	0.0	8.446081	4
TAATACG	125	2.7398928E-4	8.36162	4
CTAGTAC	365	0.0	8.330381	3
GTCTTAG	570	0.0	8.173735	1
GTATTAG	850	0.0	8.165885	1
CGAATTA	105	0.004523933	8.1412	15
TCAGTAC	505	0.0	8.090677	3
AATACTG	1015	0.0	8.050821	5
GTCCTAA	520	0.0	8.045418	1
GTTCTAT	715	0.0	7.978927	1
TATTCCG	215	8.628376E-8	7.9550295	5
AGGACCG	120	0.0016888052	7.918201	5
>>END_MODULE